Rroxscaffold_30G00449560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000030
OG0017764
Physical Location & Seq
Reverse (-)
41886 .. 42424
539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_30G00449560.1

Sequence Viewer

Length: 375 bp
ATGCCGAAGCACGCCGAGACGGCGCATGCTGCCTACCATGATCGCGTCGACGACGTCTCCACGGGCATATCAACAGCCGGGCTTTGGCCGCCGCCGCAATCCGTAACGGTCCACGCCCGAGTCGACCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACGGCGCCTCGTGGTGCGACAGGGTCCGGGCACAACGGGGCTCTCACCCTCTATGGCGCCCCCTTCCGGGGGACTTGTTCCCGGTCCGCCTCGAGGACGCTTCTCCGGACTACAATTCGGACGCCTTACGACGCCGATTCTCAAGCTGGGCTATTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

13.22

Weight (kDa)

9.23

Isoelectric Point (pI)

54.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 57
AbsI CCTCGAGG 1 cut(s) 274
AccB1I GGYRCC 2 cut(s) 187, 239
AccI GTMKAC 2 cut(s) 48, 123
AccII CGCG 1 cut(s) 45
AccIII TCCGGA 1 cut(s) 288
AciI CCGC 5 cut(s) 89, 92, 95, 143, 270
AcoI YGGCCR 1 cut(s) 86
AcsI RAATTY 1 cut(s) 136
AcyI GRCGYC 5 cut(s) 54, 188, 240, 305, 315
AfiI CCNNNNNNNGG 7 cut(s) 84, 152, 249, 250, 251, 252, 276
AluBI AGCT 1 cut(s) 329
AluI AGCT 1 cut(s) 329
Alw26I GTCTC 2 cut(s) 11, 61
Ama87I CYCGRG 2 cut(s) 117, 274
Aor13HI TCCGGA 1 cut(s) 288
AoxI GGCC 1 cut(s) 86
ApeKI GCWGC 2 cut(s) 29, 156
ApoI RAATTY 1 cut(s) 136
AspLEI GCGC 3 cut(s) 25, 190, 242
AspS9I GGNCC 3 cut(s) 109, 207, 267
AsuC2I CCSGG 5 cut(s) 79, 211, 251, 265, 341
AsuHPI GGTGA 1 cut(s) 220
AvaI CYCGRG 2 cut(s) 117, 274
AvaII GGWCC 3 cut(s) 109, 207, 267
BaeGI GKGCMC 1 cut(s) 216
BanI GGYRCC 2 cut(s) 187, 239
BanII GRGCYC 1 cut(s) 226
BauI CACGAG 1 cut(s) 192
BbvI GCAGC 2 cut(s) 16, 143
BceAI ACGGC 3 cut(s) 36, 201, 337
BcnI CCSGG 5 cut(s) 79, 211, 251, 265, 341
BcoDI GTCTC 2 cut(s) 11, 61
BfoI RGCGCY 2 cut(s) 191, 243
BglI GCCNNNNNGGC 1 cut(s) 20
BisI GCNGC 5 cut(s) 30, 89, 92, 95, 157
BlsI GCNGC 5 cut(s) 31, 90, 93, 96, 158
Bme1390I CCNGG 5 cut(s) 79, 211, 251, 265, 341
Bme18I GGWCC 3 cut(s) 109, 207, 267
BmeT110I CYCGRG 2 cut(s) 117, 274
BmgT120I GGNCC 3 cut(s) 109, 207, 267
BmiI GGNNCC 3 cut(s) 189, 208, 241
BmrFI CCNGG 5 cut(s) 79, 211, 251, 265, 341
BpuEI CTTGAG 1 cut(s) 309
BpuMI CCSGG 5 cut(s) 79, 211, 251, 265, 341
BsaHI GRCGYC 5 cut(s) 54, 188, 240, 305, 315
BsaJI CCNNGG 3 cut(s) 60, 127, 250
BsaWI WCCGGW 1 cut(s) 288
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 7 cut(s) 84, 152, 249, 250, 251, 252, 276
BseAI TCCGGA 1 cut(s) 288
BseDI CCNNGG 3 cut(s) 60, 127, 250
BseLI CCNNNNNNNGG 7 cut(s) 84, 152, 249, 250, 251, 252, 276
BseSI GKGCMC 1 cut(s) 216
BseXI GCAGC 2 cut(s) 16, 143
BseYI CCCAGC 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 1 cut(s) 88
BshNI GGYRCC 2 cut(s) 187, 239
BsiHKCI CYCGRG 2 cut(s) 117, 274
BsiSI CCGG 6 cut(s) 78, 210, 250, 265, 289, 341
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 7 cut(s) 84, 152, 249, 250, 251, 252, 276
BsmAI GTCTC 2 cut(s) 11, 61
BsmBI CGTCTC 2 cut(s) 11, 61
BsmFI GGGAC 1 cut(s) 268
BsmI GAATGC 1 cut(s) 159
BsnI GGCC 1 cut(s) 88
BsoBI CYCGRG 2 cut(s) 117, 274
Bsp1286I GDGCHC 2 cut(s) 216, 226
Bsp13I TCCGGA 1 cut(s) 288
Bsp143I GATC 1 cut(s) 40
BspACI CCGC 5 cut(s) 89, 92, 95, 143, 270
BspANI GGCC 1 cut(s) 88
BspEI TCCGGA 1 cut(s) 288
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 3 cut(s) 189, 208, 241
BspT107I GGYRCC 2 cut(s) 187, 239
BssECI CCNNGG 3 cut(s) 60, 127, 250
BssMI GATC 1 cut(s) 40
BssNI GRCGYC 5 cut(s) 54, 188, 240, 305, 315
BssSI CACGAG 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 127
Bst2BI CACGAG 1 cut(s) 192
Bst4CI ACNGT 1 cut(s) 109
BstACI GRCGYC 5 cut(s) 54, 188, 240, 305, 315
BstC8I GCNNGC 3 cut(s) 12, 27, 350
BstDEI CTNAG 1 cut(s) 359
BstDSI CCRYGG 1 cut(s) 60
BstFNI CGCG 1 cut(s) 45
BstH2I RGCGCY 2 cut(s) 191, 243
BstHHI GCGC 3 cut(s) 25, 190, 242
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 2 cut(s) 11, 61
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 5 cut(s) 20, 29, 88, 94, 187
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 5 cut(s) 77, 209, 249, 263, 339
BstSLI GKGCMC 1 cut(s) 216
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 2 cut(s) 16, 143
BsuRI GGCC 1 cut(s) 88
BtgI CCRYGG 1 cut(s) 60
Cac8I GCNNGC 3 cut(s) 12, 27, 350
CfoI GCGC 3 cut(s) 25, 190, 242
Cfr13I GGNCC 3 cut(s) 109, 207, 267
CpoI CGGWCCG 1 cut(s) 267
CseI GACGC 4 cut(s) 34, 289, 313, 323
CspI CGGWCCG 1 cut(s) 267
CviAII CATG 2 cut(s) 26, 38
CviJI RGCY 7 cut(s) 77, 82, 88, 156, 224, 329, 334
CviKI_1 RGCY 7 cut(s) 77, 82, 88, 156, 224, 329, 334
DdeI CTNAG 1 cut(s) 359
DinI GGCGCC 2 cut(s) 189, 241
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
EaeI YGGCCR 1 cut(s) 86
EciI GGCGGA 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 127
Eco24I GRGCYC 1 cut(s) 226
Eco47I GGWCC 3 cut(s) 109, 207, 267
Eco88I CYCGRG 2 cut(s) 117, 274
EcoT14I CCWWGG 1 cut(s) 127
EcoT38I GRGCYC 1 cut(s) 226
EgeI GGCGCC 2 cut(s) 189, 241
EheI GGCGCC 2 cut(s) 189, 241
ErhI CCWWGG 1 cut(s) 127
Esp3I CGTCTC 2 cut(s) 11, 61
FaeI CATG 2 cut(s) 29, 41
FaiI YATR 4 cut(s) 27, 39, 68, 237
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 25, 37
FblI GTMKAC 2 cut(s) 48, 123
Fnu4HI GCNGC 5 cut(s) 30, 89, 92, 95, 157
FriOI GRGCYC 1 cut(s) 226
Fsp4HI GCNGC 5 cut(s) 30, 89, 92, 95, 157
GlaI GCGC 3 cut(s) 24, 189, 241
GluI GCNGC 5 cut(s) 30, 89, 92, 95, 157
GsaI CCCAGC 1 cut(s) 333
HaeII RGCGCY 2 cut(s) 191, 243
HaeIII GGCC 1 cut(s) 88
HapII CCGG 6 cut(s) 78, 210, 250, 265, 289, 341
HgaI GACGC 4 cut(s) 34, 289, 313, 323
HhaI GCGC 3 cut(s) 25, 190, 242
Hin1I GRCGYC 5 cut(s) 54, 188, 240, 305, 315
Hin1II CATG 2 cut(s) 29, 41
Hin6I GCGC 3 cut(s) 23, 188, 240
HinP1I GCGC 3 cut(s) 23, 188, 240
HincII GTYRAC 2 cut(s) 49, 124
HindII GTYRAC 2 cut(s) 49, 124
HinfI GANTC 4 cut(s) 120, 176, 320, 365
HpaII CCGG 6 cut(s) 78, 210, 250, 265, 289, 341
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 3 cut(s) 49, 112, 124
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 1 cut(s) 289
Hpy8I GTNNAC 3 cut(s) 49, 112, 124
Hpy99I CGWCG 5 cut(s) 50, 53, 56, 187, 317
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 1 cut(s) 159
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 29, 88, 94, 187
HpyF3I CTNAG 1 cut(s) 359
HpySE526I ACGT 1 cut(s) 54
Hsp92I GRCGYC 5 cut(s) 54, 188, 240, 305, 315
Hsp92II CATG 2 cut(s) 29, 41
HspAI GCGC 3 cut(s) 23, 188, 240
KasI GGCGCC 2 cut(s) 187, 239
Kpn2I TCCGGA 1 cut(s) 288
Kzo9I GATC 1 cut(s) 40
LpnPI CCDG 8 cut(s) 91, 189, 223, 263, 278, 302, 315, 354
Lsp1109I GCAGC 2 cut(s) 16, 143
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 2 cut(s) 103, 354
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MhlI GDGCHC 2 cut(s) 216, 226
MluCI AATT 2 cut(s) 136, 297
Mly113I GGCGCC 2 cut(s) 188, 240
MlyI GAGTC 2 cut(s) 129, 170
MnlI CCTC 4 cut(s) 201, 242, 270, 283
MroI TCCGGA 1 cut(s) 288
MspI CCGG 6 cut(s) 78, 210, 250, 265, 289, 341
MspR9I CCNGG 5 cut(s) 79, 211, 251, 265, 341
Mva1269I GAATGC 1 cut(s) 159
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 5 cut(s) 20, 29, 88, 94, 187
NarI GGCGCC 2 cut(s) 188, 240
NciI CCSGG 5 cut(s) 79, 211, 251, 265, 341
NdeII GATC 1 cut(s) 40
NlaIII CATG 2 cut(s) 29, 41
NlaIV GGNNCC 3 cut(s) 189, 208, 241
NmeAIII GCCGAG 1 cut(s) 40
NspI RCATGY 1 cut(s) 29
PaeI GCATGC 1 cut(s) 29
PaeR7I CTCGAG 1 cut(s) 274
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 320, 365
PflFI GACNNNGTC 2 cut(s) 53, 205
PkrI GCNGC 5 cut(s) 31, 90, 93, 96, 158
PleI GAGTC 2 cut(s) 128, 170
PluTI GGCGCC 2 cut(s) 191, 243
PpsI GAGTC 2 cut(s) 128, 170
PspFI CCCAGC 1 cut(s) 329
PspN4I GGNNCC 3 cut(s) 189, 208, 241
PspPI GGNCC 3 cut(s) 109, 207, 267
PspXI VCTCGAGB 1 cut(s) 274
PsyI GACNNNGTC 2 cut(s) 53, 205
Rsr2I CGGWCCG 1 cut(s) 267
RsrII CGGWCCG 1 cut(s) 267
SalI GTCGAC 2 cut(s) 47, 122
SatI GCNGC 5 cut(s) 30, 89, 92, 95, 157
Sau3AI GATC 1 cut(s) 40
Sau96I GGNCC 3 cut(s) 109, 207, 267
SchI GAGTC 2 cut(s) 129, 170
ScrFI CCNGG 5 cut(s) 79, 211, 251, 265, 341
SduI GDGCHC 2 cut(s) 216, 226
SetI ASST 3 cut(s) 57, 129, 331
SfoI GGCGCC 2 cut(s) 189, 241
Sfr274I CTCGAG 1 cut(s) 274
SgrDI CGTCGACG 1 cut(s) 47
SinI GGWCC 3 cut(s) 109, 207, 267
SlaI CTCGAG 1 cut(s) 274
SmlI CTYRAG 2 cut(s) 274, 324
SmoI CTYRAG 2 cut(s) 274, 324
SphI GCATGC 1 cut(s) 29
Sse9I AATT 2 cut(s) 136, 297
SsiI CCGC 5 cut(s) 89, 92, 95, 143, 270
SspDI GGCGCC 2 cut(s) 187, 239
StyD4I CCNGG 5 cut(s) 77, 209, 249, 263, 339
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 1 cut(s) 57
TaqI TCGA 3 cut(s) 48, 123, 275
TasI AATT 2 cut(s) 136, 297
TauI GCSGC 3 cut(s) 91, 94, 97
TfiI GAWTC 2 cut(s) 320, 365
TseI GCWGC 2 cut(s) 29, 156
TspGWI ACGGA 2 cut(s) 91, 148
Tth111I GACNNNGTC 2 cut(s) 53, 205
VpaK11BI GGWCC 3 cut(s) 109, 207, 267
XapI RAATTY 1 cut(s) 136
XceI RCATGY 1 cut(s) 29
XhoI CTCGAG 1 cut(s) 274
XmiI GTMKAC 2 cut(s) 48, 123
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.