Rroxscaffold_80G00450290

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000080
OG0017764
Physical Location & Seq
Reverse (-)
14701 .. 15267
567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_80G00450290.1

Sequence Viewer

Length: 567 bp
ATGGGAAGCCCGCAGGCCGTTACCAGGAGCACGCAGATGCCGAAGCACGCCGAGACGGCGCATGCTGCCTACCATGATCGCGTCGACGACGTCTCCACGGGCATATCAACAGCCCGGGCTTTGGCCGCCGCCGCGATCCGTAACGGTCCACGCCCCGAGTCGAGTGGCGGACCGGCTTGTGACCGTTCCACATCCGACCGAGGCGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTACTCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACGGCGCCTCGTGGTGCGACGGGGTCCGGGCACAACGGGGCTCTCACCCTCTATGGCGCCCCCTTCCGGGGGACTTGTTCCCGGTCCGCCTCGAGGACGCTTCTCCGGTACTACAATTCGGACGCCTTACGACGCCGCATTCTCAAGCTGGGCTATTCCCGGTTCGCTCGCCGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

20.19

Weight (kDa)

10.68

Isoelectric Point (pI)

56.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AbsI CCTCGAGG 1 cut(s) 478
AccB1I GGYRCC 2 cut(s) 391, 443
AccI GTMKAC 1 cut(s) 84
AccII CGCG 3 cut(s) 81, 134, 291
AclWI GGATC 1 cut(s) 130
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 1 cut(s) 340
AcyI GRCGYC 5 cut(s) 90, 392, 444, 510, 520
AfaI GTAC 2 cut(s) 295, 497
AfiI CCNNNNNNNGG 8 cut(s) 24, 121, 356, 453, 454, 455, 456, 480
AgsI TTSAA 2 cut(s) 244, 267
AjnI CCWGG 1 cut(s) 23
AluBI AGCT 1 cut(s) 535
AluI AGCT 1 cut(s) 535
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 3 cut(s) 47, 97, 317
AlwI GGATC 1 cut(s) 130
Ama87I CYCGRG 3 cut(s) 114, 155, 478
AoxI GGCC 3 cut(s) 15, 123, 213
ApeKI GCWGC 2 cut(s) 65, 360
ApoI RAATTY 1 cut(s) 340
AspLEI GCGC 4 cut(s) 61, 206, 394, 446
AspS9I GGNCC 5 cut(s) 146, 170, 214, 411, 471
AsuC2I CCSGG 6 cut(s) 115, 116, 415, 455, 469, 547
AsuHPI GGTGA 1 cut(s) 424
AvaI CYCGRG 3 cut(s) 114, 155, 478
AvaII GGWCC 4 cut(s) 146, 170, 411, 471
BaeGI GKGCMC 1 cut(s) 420
BanI GGYRCC 2 cut(s) 391, 443
BanII GRGCYC 1 cut(s) 430
BauI CACGAG 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 52, 347
BccI CCATC 1 cut(s) 227
BceAI ACGGC 4 cut(s) 2, 72, 405, 543
BciT130I CCWGG 1 cut(s) 25
BcnI CCSGG 6 cut(s) 115, 116, 415, 455, 469, 547
BcoDI GTCTC 3 cut(s) 47, 97, 317
BfoI RGCGCY 2 cut(s) 395, 447
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 6 cut(s) 66, 126, 129, 132, 361, 523
BlsI GCNGC 6 cut(s) 67, 127, 130, 133, 362, 524
Bme1390I CCNGG 7 cut(s) 25, 115, 116, 415, 455, 469, 547
Bme18I GGWCC 4 cut(s) 146, 170, 411, 471
BmeT110I CYCGRG 3 cut(s) 114, 155, 478
BmgT120I GGNCC 5 cut(s) 146, 170, 214, 411, 471
BmiI GGNNCC 4 cut(s) 216, 393, 412, 445
BmrFI CCNGG 7 cut(s) 25, 115, 116, 415, 455, 469, 547
BmsI GCATC 2 cut(s) 27, 215
BpuEI CTTGAG 1 cut(s) 515
BpuMI CCSGG 6 cut(s) 115, 116, 415, 455, 469, 547
BsaHI GRCGYC 5 cut(s) 90, 392, 444, 510, 520
BsaI GGTCTC 1 cut(s) 317
BsaJI CCNNGG 5 cut(s) 96, 114, 199, 331, 454
BsaWI WCCGGW 1 cut(s) 492
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 8 cut(s) 24, 121, 356, 453, 454, 455, 456, 480
Bse118I RCCGGY 2 cut(s) 172, 211
BseBI CCWGG 1 cut(s) 25
BseDI CCNNGG 5 cut(s) 96, 114, 199, 331, 454
BseGI GGATG 2 cut(s) 191, 219
BseLI CCNNNNNNNGG 8 cut(s) 24, 121, 356, 453, 454, 455, 456, 480
BseSI GKGCMC 1 cut(s) 420
BseXI GCAGC 2 cut(s) 52, 347
BseYI CCCAGC 1 cut(s) 535
Bsh1236I CGCG 3 cut(s) 81, 134, 291
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 3 cut(s) 17, 125, 215
BshNI GGYRCC 2 cut(s) 391, 443
BsiEI CGRYCG 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 3 cut(s) 114, 155, 478
BsiSI CCGG 8 cut(s) 115, 173, 212, 414, 454, 469, 493, 547
BslFI GGGAC 1 cut(s) 472
BslI CCNNNNNNNGG 8 cut(s) 24, 121, 356, 453, 454, 455, 456, 480
BsmAI GTCTC 3 cut(s) 47, 97, 317
BsmBI CGTCTC 2 cut(s) 47, 97
BsmFI GGGAC 1 cut(s) 472
BsmI GAATGC 2 cut(s) 363, 525
BsnI GGCC 3 cut(s) 17, 125, 215
Bso31I GGTCTC 1 cut(s) 317
BsoBI CYCGRG 3 cut(s) 114, 155, 478
Bsp1286I GDGCHC 3 cut(s) 32, 420, 430
Bsp143I GATC 2 cut(s) 76, 135
BspANI GGCC 3 cut(s) 17, 125, 215
BspFNI CGCG 3 cut(s) 81, 134, 291
BspLI GGNNCC 4 cut(s) 216, 393, 412, 445
BspPI GGATC 1 cut(s) 130
BspT107I GGYRCC 2 cut(s) 391, 443
BspTNI GGTCTC 1 cut(s) 317
BsrFI RCCGGY 2 cut(s) 172, 211
BssAI RCCGGY 2 cut(s) 172, 211
BssECI CCNNGG 5 cut(s) 96, 114, 199, 331, 454
BssMI GATC 2 cut(s) 76, 135
BssNI GRCGYC 5 cut(s) 90, 392, 444, 510, 520
BssSI CACGAG 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 331
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 25
Bst4CI ACNGT 2 cut(s) 146, 185
BstACI GRCGYC 5 cut(s) 90, 392, 444, 510, 520
BstC8I GCNNGC 7 cut(s) 11, 15, 32, 48, 63, 213, 556
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 2 cut(s) 191, 219
BstFNI CGCG 3 cut(s) 81, 134, 291
BstH2I RGCGCY 2 cut(s) 395, 447
BstHHI GCGC 4 cut(s) 61, 206, 394, 446
BstKTI GATC 2 cut(s) 79, 138
BstMAI GTCTC 3 cut(s) 47, 97, 317
BstMBI GATC 2 cut(s) 76, 135
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 6 cut(s) 56, 65, 125, 131, 212, 391
BstNI CCWGG 1 cut(s) 25
BstNSI RCATGY 1 cut(s) 65
BstSCI CCNGG 7 cut(s) 23, 113, 114, 413, 453, 467, 545
BstSLI GKGCMC 1 cut(s) 420
BstUI CGCG 3 cut(s) 81, 134, 291
BstV1I GCAGC 2 cut(s) 52, 347
BsuRI GGCC 3 cut(s) 17, 125, 215
BtgI CCRYGG 1 cut(s) 96
BtgZI GCGATG 1 cut(s) 192
BtsCI GGATG 2 cut(s) 191, 219
Cac8I GCNNGC 7 cut(s) 11, 15, 32, 48, 63, 213, 556
CfoI GCGC 4 cut(s) 61, 206, 394, 446
Cfr10I RCCGGY 2 cut(s) 172, 211
Cfr13I GGNCC 5 cut(s) 146, 170, 214, 411, 471
Cfr9I CCCGGG 1 cut(s) 114
CpoI CGGWCCG 2 cut(s) 170, 471
CseI GACGC 4 cut(s) 70, 493, 518, 528
Csp6I GTAC 2 cut(s) 294, 496
CspI CGGWCCG 2 cut(s) 170, 471
CviAII CATG 2 cut(s) 62, 74
CviQI GTAC 2 cut(s) 294, 496
DinI GGCGCC 2 cut(s) 393, 445
DpnI GATC 2 cut(s) 78, 137
DpnII GATC 2 cut(s) 76, 135
EaeI YGGCCR 1 cut(s) 123
EciI GGCGGA 2 cut(s) 183, 463
Eco130I CCWWGG 1 cut(s) 331
Eco24I GRGCYC 1 cut(s) 430
Eco31I GGTCTC 1 cut(s) 317
Eco47I GGWCC 4 cut(s) 146, 170, 411, 471
Eco88I CYCGRG 3 cut(s) 114, 155, 478
EcoRII CCWGG 1 cut(s) 23
EcoT14I CCWWGG 1 cut(s) 331
EcoT38I GRGCYC 1 cut(s) 430
EgeI GGCGCC 2 cut(s) 393, 445
EheI GGCGCC 2 cut(s) 393, 445
ErhI CCWWGG 1 cut(s) 331
Esp3I CGTCTC 2 cut(s) 47, 97
FaeI CATG 2 cut(s) 65, 77
FaiI YATR 4 cut(s) 63, 75, 104, 441
FaqI GGGAC 1 cut(s) 472
FatI CATG 2 cut(s) 61, 73
FauI CCCGC 1 cut(s) 18
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 6 cut(s) 66, 126, 129, 132, 361, 523
FokI GGATG 2 cut(s) 178, 206
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 6 cut(s) 66, 126, 129, 132, 361, 523
GlaI GCGC 4 cut(s) 60, 205, 393, 445
GluI GCNGC 6 cut(s) 66, 126, 129, 132, 361, 523
GsaI CCCAGC 1 cut(s) 539
HaeII RGCGCY 2 cut(s) 395, 447
HaeIII GGCC 3 cut(s) 17, 125, 215
HapII CCGG 8 cut(s) 115, 173, 212, 414, 454, 469, 493, 547
HgaI GACGC 4 cut(s) 70, 493, 518, 528
HhaI GCGC 4 cut(s) 61, 206, 394, 446
Hin1I GRCGYC 5 cut(s) 90, 392, 444, 510, 520
Hin1II CATG 2 cut(s) 65, 77
Hin6I GCGC 4 cut(s) 59, 204, 392, 444
HinP1I GCGC 4 cut(s) 59, 204, 392, 444
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 3 cut(s) 158, 256, 380
HpaII CCGG 8 cut(s) 115, 173, 212, 414, 454, 469, 493, 547
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 2 cut(s) 85, 149
Hpy188I TCNGA 2 cut(s) 196, 508
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 2 cut(s) 85, 149
Hpy99I CGWCG 6 cut(s) 86, 89, 92, 391, 409, 522
HpyAV CCTTC 1 cut(s) 460
HpyCH4III ACNGT 2 cut(s) 146, 185
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 1 cut(s) 363
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 65, 125, 131, 212, 391
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 5 cut(s) 90, 392, 444, 510, 520
Hsp92II CATG 2 cut(s) 65, 77
HspAI GCGC 4 cut(s) 59, 204, 392, 444
KasI GGCGCC 2 cut(s) 391, 443
KroI GCCGGC 1 cut(s) 211
KroNI GCCGGC 1 cut(s) 213
Kzo9I GATC 2 cut(s) 76, 135
LmnI GCTCC 1 cut(s) 27
Lsp1109I GCAGC 2 cut(s) 52, 347
LweI GCATC 2 cut(s) 27, 215
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 4 cut(s) 19, 140, 179, 560
MalI GATC 2 cut(s) 78, 137
MboI GATC 2 cut(s) 76, 135
MhlI GDGCHC 3 cut(s) 32, 420, 430
MluCI AATT 3 cut(s) 238, 340, 502
Mly113I GGCGCC 2 cut(s) 392, 444
MlyI GAGTC 3 cut(s) 167, 250, 374
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 7 cut(s) 194, 240, 297, 405, 446, 474, 487
MroNI GCCGGC 1 cut(s) 211
MslI CAYNNNNRTG 1 cut(s) 35
MspI CCGG 8 cut(s) 115, 173, 212, 414, 454, 469, 493, 547
MspR9I CCNGG 7 cut(s) 25, 115, 116, 415, 455, 469, 547
Mva1269I GAATGC 2 cut(s) 363, 525
MvaI CCWGG 1 cut(s) 25
MvnI CGCG 3 cut(s) 81, 134, 291
MwoI GCNNNNNNNGC 6 cut(s) 56, 65, 125, 131, 212, 391
NaeI GCCGGC 1 cut(s) 213
NarI GGCGCC 2 cut(s) 392, 444
NciI CCSGG 6 cut(s) 115, 116, 415, 455, 469, 547
NdeII GATC 2 cut(s) 76, 135
NgoMIV GCCGGC 1 cut(s) 211
NlaIII CATG 2 cut(s) 65, 77
NlaIV GGNNCC 4 cut(s) 216, 393, 412, 445
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 179
NspI RCATGY 1 cut(s) 65
PaeI GCATGC 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PctI GAATGC 2 cut(s) 363, 525
PdiI GCCGGC 1 cut(s) 213
PflFI GACNNNGTC 2 cut(s) 89, 409
PkrI GCNGC 6 cut(s) 67, 127, 130, 133, 362, 524
PleI GAGTC 3 cut(s) 166, 250, 374
PluTI GGCGCC 2 cut(s) 395, 447
PpsI GAGTC 3 cut(s) 166, 250, 374
Psp6I CCWGG 1 cut(s) 23
PspFI CCCAGC 1 cut(s) 535
PspGI CCWGG 1 cut(s) 23
PspN4I GGNNCC 4 cut(s) 216, 393, 412, 445
PspPI GGNCC 5 cut(s) 146, 170, 214, 411, 471
PspXI VCTCGAGB 1 cut(s) 478
PsyI GACNNNGTC 2 cut(s) 89, 409
RsaI GTAC 2 cut(s) 295, 497
RsaNI GTAC 2 cut(s) 294, 496
RseI CAYNNNNRTG 1 cut(s) 35
Rsr2I CGGWCCG 2 cut(s) 170, 471
RsrII CGGWCCG 2 cut(s) 170, 471
SalI GTCGAC 1 cut(s) 83
SatI GCNGC 6 cut(s) 66, 126, 129, 132, 361, 523
Sau3AI GATC 2 cut(s) 76, 135
Sau96I GGNCC 5 cut(s) 146, 170, 214, 411, 471
SchI GAGTC 3 cut(s) 167, 250, 374
ScrFI CCNGG 7 cut(s) 25, 115, 116, 415, 455, 469, 547
SduI GDGCHC 3 cut(s) 32, 420, 430
SetI ASST 2 cut(s) 93, 537
SfaNI GCATC 2 cut(s) 27, 215
SfoI GGCGCC 2 cut(s) 393, 445
Sfr274I CTCGAG 1 cut(s) 478
SgrDI CGTCGACG 1 cut(s) 83
SinI GGWCC 4 cut(s) 146, 170, 411, 471
SlaI CTCGAG 1 cut(s) 478
SmaI CCCGGG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 478, 530
SmoI CTYRAG 2 cut(s) 478, 530
SphI GCATGC 1 cut(s) 65
SrfI GCCCGGGC 1 cut(s) 116
Sse9I AATT 3 cut(s) 238, 340, 502
SspDI GGCGCC 2 cut(s) 391, 443
StyD4I CCNGG 7 cut(s) 23, 113, 114, 413, 453, 467, 545
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 2 cut(s) 146, 185
TaiI ACGT 1 cut(s) 93
TaqI TCGA 3 cut(s) 84, 161, 479
TaqII GACCGA 2 cut(s) 213, 299
TasI AATT 3 cut(s) 238, 340, 502
TauI GCSGC 4 cut(s) 128, 131, 134, 525
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 2 cut(s) 65, 360
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 1 cut(s) 267
TspGWI ACGGA 2 cut(s) 128, 352
TspMI CCCGGG 1 cut(s) 114
Tth111I GACNNNGTC 2 cut(s) 89, 409
VpaK11BI GGWCC 4 cut(s) 146, 170, 411, 471
XapI RAATTY 1 cut(s) 340
XceI RCATGY 1 cut(s) 65
XhoI CTCGAG 1 cut(s) 478
XmaI CCCGGG 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 84
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.