Rroxscaffold_51G00447860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000051
OG0017764
Physical Location & Seq
Reverse (-)
24974 .. 25575
602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_51G00447860.1

Sequence Viewer

Length: 495 bp
ATGTCGGACTCCTTGGTCCGTGTTTCAAGACGGGCCGAATGGGGAGCCCGCGGGCCGTTACCGAGGAGCACGCAGATGCCGAAGCACGCCGAGACGGCGCATGCTGCCTACCATGATCGCGTCGACGACGTCTCCACGGGCATATCAACAGCCGGGCTTTGGCCGCCGCCGCGATCCGTAACGGTCCACGCCCGAGTCGATCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGGTGCGACGGGGTCCGGGCACAACGGGGCTCTCACCCTCTATGGCGCCCCCTTCCGGGGGACTTGTTCCCGGTCCGCCTGCGAGGACGCTTCTCCGGACTACAATTCGGACGCCTTACGACGCCGGATTCTCAAGCTGGGCTATTCCCGGTTCGCTCGCCGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

17.91

Weight (kDa)

9.95

Isoelectric Point (pI)

55.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
AatII GACGTC 1 cut(s) 132
AccB1I GGYRCC 1 cut(s) 371
AccI GTMKAC 1 cut(s) 123
AccII CGCG 4 cut(s) 51, 120, 172, 221
AccIII TCCGGA 1 cut(s) 421
AciI CCGC 8 cut(s) 49, 51, 164, 167, 170, 221, 275, 402
AclWI GGATC 2 cut(s) 168, 194
AcoI YGGCCR 1 cut(s) 161
AcsI RAATTY 1 cut(s) 268
AcyI GRCGYC 4 cut(s) 129, 372, 438, 448
AfaI GTAC 1 cut(s) 225
AfiI CCNNNNNNNGG 6 cut(s) 159, 284, 381, 382, 383, 384
AgsI TTSAA 1 cut(s) 27
AluBI AGCT 1 cut(s) 463
AluI AGCT 1 cut(s) 463
Alw21I GWGCWC 1 cut(s) 71
Alw26I GTCTC 3 cut(s) 86, 136, 246
AlwI GGATC 2 cut(s) 168, 194
Ama87I CYCGRG 1 cut(s) 192
Aor13HI TCCGGA 1 cut(s) 421
AoxI GGCC 3 cut(s) 33, 53, 161
ApeKI GCWGC 2 cut(s) 104, 288
ApoI RAATTY 1 cut(s) 268
AspLEI GCGC 3 cut(s) 100, 322, 374
AspS9I GGNCC 6 cut(s) 16, 33, 53, 184, 339, 399
AsuC2I CCSGG 5 cut(s) 154, 343, 383, 397, 475
AsuHPI GGTGA 1 cut(s) 352
AvaI CYCGRG 1 cut(s) 192
AvaII GGWCC 4 cut(s) 16, 184, 339, 399
BaeGI GKGCMC 1 cut(s) 348
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 2 cut(s) 49, 358
BauI CACGAG 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 71
BbvI GCAGC 2 cut(s) 91, 275
BceAI ACGGC 4 cut(s) 40, 111, 233, 471
BcnI CCSGG 5 cut(s) 154, 343, 383, 397, 475
BcoDI GTCTC 3 cut(s) 86, 136, 246
BfoI RGCGCY 2 cut(s) 323, 375
BglI GCCNNNNNGGC 1 cut(s) 95
BisI GCNGC 5 cut(s) 105, 164, 167, 170, 289
BlsI GCNGC 5 cut(s) 106, 165, 168, 171, 290
Bme1390I CCNGG 5 cut(s) 154, 343, 383, 397, 475
Bme18I GGWCC 4 cut(s) 16, 184, 339, 399
BmeT110I CYCGRG 1 cut(s) 192
BmgT120I GGNCC 6 cut(s) 16, 33, 53, 184, 339, 399
BmiI GGNNCC 3 cut(s) 46, 340, 373
BmrFI CCNGG 5 cut(s) 154, 343, 383, 397, 475
BmsI GCATC 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 5 cut(s) 154, 343, 383, 397, 475
BsaHI GRCGYC 4 cut(s) 129, 372, 438, 448
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 6 cut(s) 12, 49, 62, 135, 259, 382
BsaWI WCCGGW 1 cut(s) 421
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 6 cut(s) 159, 284, 381, 382, 383, 384
BseAI TCCGGA 1 cut(s) 421
BseDI CCNNGG 6 cut(s) 12, 49, 62, 135, 259, 382
BseLI CCNNNNNNNGG 6 cut(s) 159, 284, 381, 382, 383, 384
BseRI GAGGAG 1 cut(s) 79
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 2 cut(s) 91, 275
BseYI CCCAGC 1 cut(s) 463
Bsh1236I CGCG 4 cut(s) 51, 120, 172, 221
BshFI GGCC 3 cut(s) 35, 55, 163
BshNI GGYRCC 1 cut(s) 371
BsiHKAI GWGCWC 1 cut(s) 71
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 7 cut(s) 153, 342, 382, 397, 422, 451, 475
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 6 cut(s) 159, 284, 381, 382, 383, 384
BsmAI GTCTC 3 cut(s) 86, 136, 246
BsmBI CGTCTC 2 cut(s) 86, 136
BsmFI GGGAC 1 cut(s) 400
BsmI GAATGC 1 cut(s) 291
BsnI GGCC 3 cut(s) 35, 55, 163
Bso31I GGTCTC 1 cut(s) 246
BsoBI CYCGRG 1 cut(s) 192
Bsp1286I GDGCHC 4 cut(s) 49, 71, 348, 358
Bsp13I TCCGGA 1 cut(s) 421
Bsp143I GATC 3 cut(s) 115, 173, 199
BspACI CCGC 8 cut(s) 49, 51, 164, 167, 170, 221, 275, 402
BspANI GGCC 3 cut(s) 35, 55, 163
BspEI TCCGGA 1 cut(s) 421
BspFNI CGCG 4 cut(s) 51, 120, 172, 221
BspLI GGNNCC 3 cut(s) 46, 340, 373
BspPI GGATC 2 cut(s) 168, 194
BspT107I GGYRCC 1 cut(s) 371
BspTNI GGTCTC 1 cut(s) 246
BssECI CCNNGG 6 cut(s) 12, 49, 62, 135, 259, 382
BssMI GATC 3 cut(s) 115, 173, 199
BssNI GRCGYC 4 cut(s) 129, 372, 438, 448
BssSI CACGAG 1 cut(s) 324
BssT1I CCWWGG 2 cut(s) 12, 259
Bst2BI CACGAG 1 cut(s) 324
Bst4CI ACNGT 1 cut(s) 184
BstACI GRCGYC 4 cut(s) 129, 372, 438, 448
BstC8I GCNNGC 7 cut(s) 49, 53, 71, 87, 102, 406, 484
BstDSI CCRYGG 2 cut(s) 49, 135
BstFNI CGCG 4 cut(s) 51, 120, 172, 221
BstH2I RGCGCY 2 cut(s) 323, 375
BstHHI GCGC 3 cut(s) 100, 322, 374
BstKTI GATC 3 cut(s) 118, 176, 202
BstMAI GTCTC 3 cut(s) 86, 136, 246
BstMBI GATC 3 cut(s) 115, 173, 199
BstMWI GCNNNNNNNGC 5 cut(s) 95, 104, 163, 169, 319
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 5 cut(s) 152, 341, 381, 395, 473
BstSLI GKGCMC 1 cut(s) 348
BstUI CGCG 4 cut(s) 51, 120, 172, 221
BstV1I GCAGC 2 cut(s) 91, 275
BsuRI GGCC 3 cut(s) 35, 55, 163
BtgI CCRYGG 2 cut(s) 49, 135
Cac8I GCNNGC 7 cut(s) 49, 53, 71, 87, 102, 406, 484
CfoI GCGC 3 cut(s) 100, 322, 374
Cfr13I GGNCC 6 cut(s) 16, 33, 53, 184, 339, 399
Cfr42I CCGCGG 1 cut(s) 52
CpoI CGGWCCG 1 cut(s) 399
CseI GACGC 4 cut(s) 109, 422, 446, 456
Csp6I GTAC 1 cut(s) 224
CspI CGGWCCG 1 cut(s) 399
CviAII CATG 2 cut(s) 101, 113
CviQI GTAC 1 cut(s) 224
DinI GGCGCC 1 cut(s) 373
DpnI GATC 3 cut(s) 117, 175, 201
DpnII GATC 3 cut(s) 115, 173, 199
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
EaeI YGGCCR 1 cut(s) 161
EciI GGCGGA 1 cut(s) 391
Eco130I CCWWGG 2 cut(s) 12, 259
Eco24I GRGCYC 2 cut(s) 49, 358
Eco31I GGTCTC 1 cut(s) 246
Eco47I GGWCC 4 cut(s) 16, 184, 339, 399
Eco88I CYCGRG 1 cut(s) 192
EcoT14I CCWWGG 2 cut(s) 12, 259
EcoT38I GRGCYC 2 cut(s) 49, 358
EgeI GGCGCC 1 cut(s) 373
EheI GGCGCC 1 cut(s) 373
ErhI CCWWGG 2 cut(s) 12, 259
Esp3I CGTCTC 2 cut(s) 86, 136
FaeI CATG 2 cut(s) 104, 116
FaiI YATR 4 cut(s) 102, 114, 143, 369
FaqI GGGAC 1 cut(s) 400
FatI CATG 2 cut(s) 100, 112
FauI CCCGC 2 cut(s) 44, 56
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 5 cut(s) 105, 164, 167, 170, 289
FriOI GRGCYC 2 cut(s) 49, 358
Fsp4HI GCNGC 5 cut(s) 105, 164, 167, 170, 289
GlaI GCGC 3 cut(s) 99, 321, 373
GluI GCNGC 5 cut(s) 105, 164, 167, 170, 289
GsaI CCCAGC 1 cut(s) 467
HaeII RGCGCY 2 cut(s) 323, 375
HaeIII GGCC 3 cut(s) 35, 55, 163
HapII CCGG 7 cut(s) 153, 342, 382, 397, 422, 451, 475
HgaI GACGC 4 cut(s) 109, 422, 446, 456
HhaI GCGC 3 cut(s) 100, 322, 374
Hin1I GRCGYC 4 cut(s) 129, 372, 438, 448
Hin1II CATG 2 cut(s) 104, 116
Hin6I GCGC 3 cut(s) 98, 320, 372
HinP1I GCGC 3 cut(s) 98, 320, 372
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 4 cut(s) 8, 195, 308, 454
HpaII CCGG 7 cut(s) 153, 342, 382, 397, 422, 451, 475
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 2 cut(s) 124, 187
Hpy188I TCNGA 2 cut(s) 7, 436
Hpy188III TCNNGA 2 cut(s) 27, 422
Hpy8I GTNNAC 2 cut(s) 124, 187
Hpy99I CGWCG 5 cut(s) 125, 128, 131, 337, 450
HpyAV CCTTC 1 cut(s) 388
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 1 cut(s) 291
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 104, 163, 169, 319
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 4 cut(s) 129, 372, 438, 448
Hsp92II CATG 2 cut(s) 104, 116
HspAI GCGC 3 cut(s) 98, 320, 372
KasI GGCGCC 1 cut(s) 371
Kpn2I TCCGGA 1 cut(s) 421
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 3 cut(s) 115, 173, 199
LmnI GCTCC 2 cut(s) 44, 66
LpnPI CCDG 9 cut(s) 166, 355, 395, 410, 418, 435, 449, 464, 488
Lsp1109I GCAGC 2 cut(s) 91, 275
LweI GCATC 1 cut(s) 66
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 3 cut(s) 57, 178, 488
MalI GATC 3 cut(s) 117, 175, 201
MboI GATC 3 cut(s) 115, 173, 199
MhlI GDGCHC 4 cut(s) 49, 71, 348, 358
MluCI AATT 2 cut(s) 268, 430
Mly113I GGCGCC 1 cut(s) 372
MlyI GAGTC 3 cut(s) 2, 204, 302
MnlI CCTC 5 cut(s) 57, 227, 333, 374, 403
MroI TCCGGA 1 cut(s) 421
MslI CAYNNNNRTG 1 cut(s) 74
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 7 cut(s) 153, 342, 382, 397, 422, 451, 475
MspR9I CCNGG 5 cut(s) 154, 343, 383, 397, 475
Mva1269I GAATGC 1 cut(s) 291
MvnI CGCG 4 cut(s) 51, 120, 172, 221
MwoI GCNNNNNNNGC 5 cut(s) 95, 104, 163, 169, 319
NarI GGCGCC 1 cut(s) 372
NciI CCSGG 5 cut(s) 154, 343, 383, 397, 475
NdeII GATC 3 cut(s) 115, 173, 199
NlaIII CATG 2 cut(s) 104, 116
NlaIV GGNNCC 3 cut(s) 46, 340, 373
NmeAIII GCCGAG 1 cut(s) 115
NspI RCATGY 1 cut(s) 104
PaeI GCATGC 1 cut(s) 104
PcsI WCGNNNNNNNCGW 2 cut(s) 77, 195
PctI GAATGC 1 cut(s) 291
PfeI GAWTC 1 cut(s) 454
PflFI GACNNNGTC 2 cut(s) 128, 337
PkrI GCNGC 5 cut(s) 106, 165, 168, 171, 290
PleI GAGTC 3 cut(s) 2, 203, 302
PluTI GGCGCC 1 cut(s) 375
PpsI GAGTC 3 cut(s) 2, 203, 302
PspFI CCCAGC 1 cut(s) 463
PspN4I GGNNCC 3 cut(s) 46, 340, 373
PspPI GGNCC 6 cut(s) 16, 33, 53, 184, 339, 399
PsyI GACNNNGTC 2 cut(s) 128, 337
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
RseI CAYNNNNRTG 1 cut(s) 74
Rsr2I CGGWCCG 1 cut(s) 399
RsrII CGGWCCG 1 cut(s) 399
SacII CCGCGG 1 cut(s) 52
SalI GTCGAC 1 cut(s) 122
SatI GCNGC 5 cut(s) 105, 164, 167, 170, 289
Sau3AI GATC 3 cut(s) 115, 173, 199
Sau96I GGNCC 6 cut(s) 16, 33, 53, 184, 339, 399
SchI GAGTC 3 cut(s) 2, 204, 302
ScrFI CCNGG 5 cut(s) 154, 343, 383, 397, 475
SduI GDGCHC 4 cut(s) 49, 71, 348, 358
SetI ASST 2 cut(s) 132, 465
SfaNI GCATC 1 cut(s) 66
SfoI GGCGCC 1 cut(s) 373
Sfr303I CCGCGG 1 cut(s) 52
SgrBI CCGCGG 1 cut(s) 52
SgrDI CGTCGACG 1 cut(s) 122
SinI GGWCC 4 cut(s) 16, 184, 339, 399
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
SphI GCATGC 1 cut(s) 104
Sse9I AATT 2 cut(s) 268, 430
SsiI CCGC 8 cut(s) 49, 51, 164, 167, 170, 221, 275, 402
SspDI GGCGCC 1 cut(s) 371
StyD4I CCNGG 5 cut(s) 152, 341, 381, 395, 473
StyI CCWWGG 2 cut(s) 12, 259
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 1 cut(s) 132
TaqI TCGA 2 cut(s) 123, 198
TaqII GACCGA 1 cut(s) 228
TasI AATT 2 cut(s) 268, 430
TauI GCSGC 3 cut(s) 166, 169, 172
TfiI GAWTC 1 cut(s) 454
TseI GCWGC 2 cut(s) 104, 288
TspDTI ATGAA 1 cut(s) 197
TspGWI ACGGA 3 cut(s) 8, 166, 280
Tth111I GACNNNGTC 2 cut(s) 128, 337
VpaK11BI GGWCC 4 cut(s) 16, 184, 339, 399
XapI RAATTY 1 cut(s) 268
XceI RCATGY 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 123
ZraI GACGTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.