Rroxscaffold_49G00438800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000049
OG0017764
Physical Location & Seq
Reverse (-)
173100 .. 173664
565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_49G00438800.1

Sequence Viewer

Length: 396 bp
ATGGGGAGCCCGCAGGCCGTTACCAGGAGCACGCAGATGCCGAAGCACGCCGAGACGGCGCATGCTGCCTACCATGATCGCGTCGACGACGTCTCCACGGGCATATCAACAGCCGGGCTTTGGCCGCCGCCGCAATCCGTAACGGTCCACGCCCGAGTCGACCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACAACGGGGCTCTCACCCTCTATGGCGCCCCCTTCCGGGGGACTTGTTCCCGGTCCGCCTCGAGGACGCTTCTCCGGACTACAATTCGGACGCCTTACGACGCCGATTCTCAAGCTGGGCTATTCCGGTTCGCTCGCCGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

131

Amino Acids

14.07

Weight (kDa)

10.01

Isoelectric Point (pI)

50.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AbsI CCTCGAGG 1 cut(s) 310
AccB1I GGYRCC 1 cut(s) 275
AccI GTMKAC 2 cut(s) 84, 159
AccII CGCG 1 cut(s) 81
AccIII TCCGGA 1 cut(s) 324
AciI CCGC 6 cut(s) 11, 125, 128, 131, 179, 306
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 1 cut(s) 172
AcyI GRCGYC 4 cut(s) 90, 276, 341, 351
AfiI CCNNNNNNNGG 8 cut(s) 24, 120, 188, 285, 286, 287, 288, 312
AjnI CCWGG 1 cut(s) 23
AluBI AGCT 1 cut(s) 365
AluI AGCT 1 cut(s) 365
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 47, 97
Ama87I CYCGRG 2 cut(s) 153, 310
Aor13HI TCCGGA 1 cut(s) 324
AoxI GGCC 2 cut(s) 15, 122
ApeKI GCWGC 2 cut(s) 65, 192
ApoI RAATTY 1 cut(s) 172
AspLEI GCGC 3 cut(s) 61, 226, 278
AspS9I GGNCC 3 cut(s) 145, 243, 303
AsuC2I CCSGG 4 cut(s) 115, 247, 287, 301
AsuHPI GGTGA 1 cut(s) 256
AvaI CYCGRG 2 cut(s) 153, 310
AvaII GGWCC 3 cut(s) 145, 243, 303
BaeGI GKGCMC 1 cut(s) 252
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 2 cut(s) 11, 262
BauI CACGAG 1 cut(s) 228
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 52, 179
BceAI ACGGC 3 cut(s) 2, 72, 372
BciT130I CCWGG 1 cut(s) 25
BcnI CCSGG 4 cut(s) 115, 247, 287, 301
BcoDI GTCTC 2 cut(s) 47, 97
BfoI RGCGCY 2 cut(s) 227, 279
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 5 cut(s) 66, 125, 128, 131, 193
BlsI GCNGC 5 cut(s) 67, 126, 129, 132, 194
Bme1390I CCNGG 5 cut(s) 25, 115, 247, 287, 301
Bme18I GGWCC 3 cut(s) 145, 243, 303
BmeT110I CYCGRG 2 cut(s) 153, 310
BmgT120I GGNCC 3 cut(s) 145, 243, 303
BmiI GGNNCC 3 cut(s) 8, 244, 277
BmrFI CCNGG 5 cut(s) 25, 115, 247, 287, 301
BmsI GCATC 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 345
BpuMI CCSGG 4 cut(s) 115, 247, 287, 301
BsaHI GRCGYC 4 cut(s) 90, 276, 341, 351
BsaJI CCNNGG 3 cut(s) 96, 163, 286
BsaWI WCCGGW 2 cut(s) 324, 375
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 8 cut(s) 24, 120, 188, 285, 286, 287, 288, 312
BseAI TCCGGA 1 cut(s) 324
BseBI CCWGG 1 cut(s) 25
BseDI CCNNGG 3 cut(s) 96, 163, 286
BseLI CCNNNNNNNGG 8 cut(s) 24, 120, 188, 285, 286, 287, 288, 312
BseSI GKGCMC 1 cut(s) 252
BseXI GCAGC 2 cut(s) 52, 179
BseYI CCCAGC 1 cut(s) 365
Bsh1236I CGCG 1 cut(s) 81
BshFI GGCC 2 cut(s) 17, 124
BshNI GGYRCC 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 2 cut(s) 153, 310
BsiSI CCGG 6 cut(s) 114, 246, 286, 301, 325, 376
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 8 cut(s) 24, 120, 188, 285, 286, 287, 288, 312
BsmAI GTCTC 2 cut(s) 47, 97
BsmBI CGTCTC 2 cut(s) 47, 97
BsmFI GGGAC 1 cut(s) 304
BsmI GAATGC 1 cut(s) 195
BsnI GGCC 2 cut(s) 17, 124
BsoBI CYCGRG 2 cut(s) 153, 310
Bsp1286I GDGCHC 4 cut(s) 11, 32, 252, 262
Bsp13I TCCGGA 1 cut(s) 324
Bsp143I GATC 1 cut(s) 76
BspACI CCGC 6 cut(s) 11, 125, 128, 131, 179, 306
BspANI GGCC 2 cut(s) 17, 124
BspEI TCCGGA 1 cut(s) 324
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 3 cut(s) 8, 244, 277
BspT107I GGYRCC 1 cut(s) 275
BssECI CCNNGG 3 cut(s) 96, 163, 286
BssMI GATC 1 cut(s) 76
BssNI GRCGYC 4 cut(s) 90, 276, 341, 351
BssSI CACGAG 1 cut(s) 228
BssT1I CCWWGG 1 cut(s) 163
Bst2BI CACGAG 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 25
Bst4CI ACNGT 1 cut(s) 145
BstACI GRCGYC 4 cut(s) 90, 276, 341, 351
BstC8I GCNNGC 6 cut(s) 11, 15, 32, 48, 63, 385
BstDSI CCRYGG 1 cut(s) 96
BstFNI CGCG 1 cut(s) 81
BstH2I RGCGCY 2 cut(s) 227, 279
BstHHI GCGC 3 cut(s) 61, 226, 278
BstKTI GATC 1 cut(s) 79
BstMAI GTCTC 2 cut(s) 47, 97
BstMBI GATC 1 cut(s) 76
BstMWI GCNNNNNNNGC 5 cut(s) 56, 65, 124, 130, 223
BstNI CCWGG 1 cut(s) 25
BstNSI RCATGY 1 cut(s) 65
BstSCI CCNGG 5 cut(s) 23, 113, 245, 285, 299
BstSLI GKGCMC 1 cut(s) 252
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 2 cut(s) 52, 179
BsuRI GGCC 2 cut(s) 17, 124
BtgI CCRYGG 1 cut(s) 96
Cac8I GCNNGC 6 cut(s) 11, 15, 32, 48, 63, 385
CfoI GCGC 3 cut(s) 61, 226, 278
Cfr13I GGNCC 3 cut(s) 145, 243, 303
CpoI CGGWCCG 1 cut(s) 303
CseI GACGC 4 cut(s) 70, 325, 349, 359
CspI CGGWCCG 1 cut(s) 303
CviAII CATG 2 cut(s) 62, 74
CviJI RGCY 9 cut(s) 9, 17, 113, 118, 124, 192, 260, 365, 370
CviKI_1 RGCY 9 cut(s) 9, 17, 113, 118, 124, 192, 260, 365, 370
DinI GGCGCC 1 cut(s) 277
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 122
EciI GGCGGA 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 163
Eco24I GRGCYC 2 cut(s) 11, 262
Eco47I GGWCC 3 cut(s) 145, 243, 303
Eco88I CYCGRG 2 cut(s) 153, 310
EcoRII CCWGG 1 cut(s) 23
EcoT14I CCWWGG 1 cut(s) 163
EcoT38I GRGCYC 2 cut(s) 11, 262
EgeI GGCGCC 1 cut(s) 277
EheI GGCGCC 1 cut(s) 277
ErhI CCWWGG 1 cut(s) 163
Esp3I CGTCTC 2 cut(s) 47, 97
FaeI CATG 2 cut(s) 65, 77
FaiI YATR 4 cut(s) 63, 75, 104, 273
FaqI GGGAC 1 cut(s) 304
FatI CATG 2 cut(s) 61, 73
FauI CCCGC 1 cut(s) 18
FblI GTMKAC 2 cut(s) 84, 159
Fnu4HI GCNGC 5 cut(s) 66, 125, 128, 131, 193
FriOI GRGCYC 2 cut(s) 11, 262
Fsp4HI GCNGC 5 cut(s) 66, 125, 128, 131, 193
GlaI GCGC 3 cut(s) 60, 225, 277
GluI GCNGC 5 cut(s) 66, 125, 128, 131, 193
GsaI CCCAGC 1 cut(s) 369
HaeII RGCGCY 2 cut(s) 227, 279
HaeIII GGCC 2 cut(s) 17, 124
HapII CCGG 6 cut(s) 114, 246, 286, 301, 325, 376
HgaI GACGC 4 cut(s) 70, 325, 349, 359
HhaI GCGC 3 cut(s) 61, 226, 278
Hin1I GRCGYC 4 cut(s) 90, 276, 341, 351
Hin1II CATG 2 cut(s) 65, 77
Hin6I GCGC 3 cut(s) 59, 224, 276
HinP1I GCGC 3 cut(s) 59, 224, 276
HincII GTYRAC 2 cut(s) 85, 160
HindII GTYRAC 2 cut(s) 85, 160
HinfI GANTC 3 cut(s) 156, 212, 356
HpaII CCGG 6 cut(s) 114, 246, 286, 301, 325, 376
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 3 cut(s) 85, 148, 160
Hpy188I TCNGA 1 cut(s) 339
Hpy188III TCNNGA 1 cut(s) 325
Hpy8I GTNNAC 3 cut(s) 85, 148, 160
Hpy99I CGWCG 4 cut(s) 86, 89, 92, 353
HpyAV CCTTC 1 cut(s) 292
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 65, 124, 130, 223
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 4 cut(s) 90, 276, 341, 351
Hsp92II CATG 2 cut(s) 65, 77
HspAI GCGC 3 cut(s) 59, 224, 276
KasI GGCGCC 1 cut(s) 275
Kpn2I TCCGGA 1 cut(s) 324
Kzo9I GATC 1 cut(s) 76
LmnI GCTCC 2 cut(s) 6, 27
Lsp1109I GCAGC 2 cut(s) 52, 179
LweI GCATC 1 cut(s) 27
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 3 cut(s) 19, 139, 389
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MhlI GDGCHC 4 cut(s) 11, 32, 252, 262
MluCI AATT 2 cut(s) 172, 333
Mly113I GGCGCC 1 cut(s) 276
MlyI GAGTC 2 cut(s) 165, 206
MnlI CCTC 4 cut(s) 237, 278, 306, 319
MroI TCCGGA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 35
MspI CCGG 6 cut(s) 114, 246, 286, 301, 325, 376
MspR9I CCNGG 5 cut(s) 25, 115, 247, 287, 301
Mva1269I GAATGC 1 cut(s) 195
MvaI CCWGG 1 cut(s) 25
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 5 cut(s) 56, 65, 124, 130, 223
NarI GGCGCC 1 cut(s) 276
NciI CCSGG 4 cut(s) 115, 247, 287, 301
NdeII GATC 1 cut(s) 76
NlaIII CATG 2 cut(s) 65, 77
NlaIV GGNNCC 3 cut(s) 8, 244, 277
NmeAIII GCCGAG 1 cut(s) 76
NspI RCATGY 1 cut(s) 65
PaeI GCATGC 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 310
PcsI WCGNNNNNNNCGW 2 cut(s) 38, 156
PctI GAATGC 1 cut(s) 195
PfeI GAWTC 1 cut(s) 356
PflFI GACNNNGTC 2 cut(s) 89, 241
PkrI GCNGC 5 cut(s) 67, 126, 129, 132, 194
PleI GAGTC 2 cut(s) 164, 206
PluTI GGCGCC 1 cut(s) 279
PpsI GAGTC 2 cut(s) 164, 206
Psp6I CCWGG 1 cut(s) 23
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 1 cut(s) 23
PspN4I GGNNCC 3 cut(s) 8, 244, 277
PspPI GGNCC 3 cut(s) 145, 243, 303
PspXI VCTCGAGB 1 cut(s) 310
PsyI GACNNNGTC 2 cut(s) 89, 241
RseI CAYNNNNRTG 1 cut(s) 35
Rsr2I CGGWCCG 1 cut(s) 303
RsrII CGGWCCG 1 cut(s) 303
SalI GTCGAC 2 cut(s) 83, 158
SatI GCNGC 5 cut(s) 66, 125, 128, 131, 193
Sau3AI GATC 1 cut(s) 76
Sau96I GGNCC 3 cut(s) 145, 243, 303
SchI GAGTC 2 cut(s) 165, 206
ScrFI CCNGG 5 cut(s) 25, 115, 247, 287, 301
SduI GDGCHC 4 cut(s) 11, 32, 252, 262
SetI ASST 3 cut(s) 93, 165, 367
SfaNI GCATC 1 cut(s) 27
SfoI GGCGCC 1 cut(s) 277
Sfr274I CTCGAG 1 cut(s) 310
SgrDI CGTCGACG 1 cut(s) 83
SinI GGWCC 3 cut(s) 145, 243, 303
SlaI CTCGAG 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 310, 360
SmoI CTYRAG 2 cut(s) 310, 360
SphI GCATGC 1 cut(s) 65
Sse9I AATT 2 cut(s) 172, 333
SsiI CCGC 6 cut(s) 11, 125, 128, 131, 179, 306
SspDI GGCGCC 1 cut(s) 275
StyD4I CCNGG 5 cut(s) 23, 113, 245, 285, 299
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 1 cut(s) 93
TaqI TCGA 3 cut(s) 84, 159, 311
TasI AATT 2 cut(s) 172, 333
TauI GCSGC 3 cut(s) 127, 130, 133
TfiI GAWTC 1 cut(s) 356
TseI GCWGC 2 cut(s) 65, 192
TspGWI ACGGA 2 cut(s) 127, 184
Tth111I GACNNNGTC 2 cut(s) 89, 241
VpaK11BI GGWCC 3 cut(s) 145, 243, 303
XapI RAATTY 1 cut(s) 172
XceI RCATGY 1 cut(s) 65
XhoI CTCGAG 1 cut(s) 310
XmiI GTMKAC 2 cut(s) 84, 159
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.