Rroxscaffold_93G00440890

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000093
OG0017764
Physical Location & Seq
Forward (+)
44779 .. 45894
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_93G00440890.1

Sequence Viewer

Length: 480 bp
ATGGAACCTTTCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGCTCACGCCCAGGTTTTGCAGCCGACCGCCGCGCCCCTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGTATAGGTCACGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGACGGGCCGAATGGGGAGCCCGCAGCCGTTACCAGGAGCACGCAGATGCCGAAGCACGCCGAGACGGCGCATGCTGCCTACCATGATCGCGTCGACGACGTCTCCACGGGCATATCAACAGCCCGGGCTTTGGCCGCCGCCGCAATCCGTAACGGTCCACGCCCCGAGTCGAGTGGCGGACCGGCTTGTGACCGTTCCACATCCGACCGAGGCGCATCGCCGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

16.36

Weight (kDa)

9.84

Isoelectric Point (pI)

59.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 318
AccBSI CCGCTC 1 cut(s) 80
AccI GTMKAC 1 cut(s) 309
AccII CGCG 3 cut(s) 120, 176, 306
AcoI YGGCCR 3 cut(s) 75, 159, 348
AcyI GRCGYC 1 cut(s) 315
AfiI CCNNNNNNNGG 4 cut(s) 161, 197, 249, 346
AflII CTTAAG 1 cut(s) 179
AgsI TTSAA 3 cut(s) 27, 41, 468
AjnI CCWGG 3 cut(s) 97, 141, 248
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 2 cut(s) 272, 322
Ama87I CYCGRG 2 cut(s) 339, 380
AoxI GGCC 6 cut(s) 20, 75, 139, 159, 221, 348
ApeKI GCWGC 3 cut(s) 107, 239, 290
AspLEI GCGC 5 cut(s) 122, 178, 186, 286, 431
AspS9I GGNCC 4 cut(s) 139, 221, 371, 395
AsuC2I CCSGG 3 cut(s) 87, 340, 341
AvaI CYCGRG 2 cut(s) 339, 380
AvaII GGWCC 2 cut(s) 371, 395
BanII GRGCYC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 257
BbvI GCAGC 3 cut(s) 119, 251, 277
BccI CCATC 2 cut(s) 195, 451
BceAI ACGGC 4 cut(s) 90, 174, 227, 297
BciT130I CCWGG 3 cut(s) 99, 143, 250
BcnI CCSGG 3 cut(s) 87, 340, 341
BcoDI GTCTC 2 cut(s) 272, 322
BfaI CTAG 1 cut(s) 203
BfoI RGCGCY 1 cut(s) 187
BfrI CTTAAG 1 cut(s) 179
BglI GCCNNNNNGGC 2 cut(s) 158, 281
BglII AGATCT 1 cut(s) 62
BisI GCNGC 9 cut(s) 78, 108, 118, 240, 291, 351, 354, 357, 437
BlsI GCNGC 9 cut(s) 79, 109, 119, 241, 292, 352, 355, 358, 438
Bme1390I CCNGG 6 cut(s) 87, 99, 143, 250, 340, 341
Bme18I GGWCC 2 cut(s) 371, 395
BmeT110I CYCGRG 2 cut(s) 339, 380
BmgT120I GGNCC 4 cut(s) 139, 221, 371, 395
BmiI GGNNCC 3 cut(s) 6, 140, 234
BmrFI CCNGG 6 cut(s) 87, 99, 143, 250, 340, 341
BmsI GCATC 2 cut(s) 252, 440
BpuMI CCSGG 3 cut(s) 87, 340, 341
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 4 cut(s) 97, 321, 339, 424
BsaXI ACNNNNNCTCC 4 cut(s) 64, 94, 302, 332
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 197, 249, 346
Bse118I RCCGGY 2 cut(s) 161, 397
BseBI CCWGG 3 cut(s) 99, 143, 250
BseDI CCNNGG 4 cut(s) 97, 321, 339, 424
BseGI GGATG 3 cut(s) 187, 416, 443
BseLI CCNNNNNNNGG 4 cut(s) 161, 197, 249, 346
BseX3I CGGCCG 2 cut(s) 75, 159
BseXI GCAGC 3 cut(s) 119, 251, 277
BsgI GTGCAG 1 cut(s) 51
Bsh1236I CGCG 3 cut(s) 120, 176, 306
Bsh1285I CGRYCG 4 cut(s) 78, 115, 162, 424
BshFI GGCC 6 cut(s) 22, 77, 141, 161, 223, 350
BsiEI CGRYCG 4 cut(s) 78, 115, 162, 424
BsiHKAI GWGCWC 1 cut(s) 257
BsiHKCI CYCGRG 2 cut(s) 339, 380
BsiSI CCGG 4 cut(s) 87, 162, 340, 398
BslI CCNNNNNNNGG 4 cut(s) 161, 197, 249, 346
BsmAI GTCTC 2 cut(s) 272, 322
BsmBI CGTCTC 2 cut(s) 272, 322
BsnI GGCC 6 cut(s) 22, 77, 141, 161, 223, 350
BsoBI CYCGRG 2 cut(s) 339, 380
Bsp1286I GDGCHC 2 cut(s) 237, 257
Bsp143I GATC 2 cut(s) 62, 301
BspANI GGCC 6 cut(s) 22, 77, 141, 161, 223, 350
BspFNI CGCG 3 cut(s) 120, 176, 306
BspLI GGNNCC 3 cut(s) 6, 140, 234
BspTI CTTAAG 1 cut(s) 179
BsrBI CCGCTC 1 cut(s) 80
BsrFI RCCGGY 2 cut(s) 161, 397
BssAI RCCGGY 2 cut(s) 161, 397
BssECI CCNNGG 4 cut(s) 97, 321, 339, 424
BssMI GATC 2 cut(s) 62, 301
BssNI GRCGYC 1 cut(s) 315
Bst2UI CCWGG 3 cut(s) 99, 143, 250
Bst4CI ACNGT 2 cut(s) 371, 410
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 315
BstAFI CTTAAG 1 cut(s) 179
BstC8I GCNNGC 4 cut(s) 237, 257, 273, 288
BstDSI CCRYGG 1 cut(s) 321
BstF5I GGATG 3 cut(s) 187, 416, 443
BstFNI CGCG 3 cut(s) 120, 176, 306
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 5 cut(s) 122, 178, 186, 286, 431
BstKTI GATC 2 cut(s) 65, 304
BstMAI GTCTC 2 cut(s) 272, 322
BstMBI GATC 2 cut(s) 62, 301
BstMCI CGRYCG 4 cut(s) 78, 115, 162, 424
BstMWI GCNNNNNNNGC 6 cut(s) 74, 158, 281, 290, 350, 356
BstNI CCWGG 3 cut(s) 99, 143, 250
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 6 cut(s) 85, 97, 141, 248, 338, 339
BstUI CGCG 3 cut(s) 120, 176, 306
BstV1I GCAGC 3 cut(s) 119, 251, 277
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BstZI CGGCCG 2 cut(s) 75, 159
BsuRI GGCC 6 cut(s) 22, 77, 141, 161, 223, 350
BtgI CCRYGG 1 cut(s) 321
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 3 cut(s) 187, 416, 443
Cac8I GCNNGC 4 cut(s) 237, 257, 273, 288
CfoI GCGC 5 cut(s) 122, 178, 186, 286, 431
Cfr10I RCCGGY 2 cut(s) 161, 397
Cfr13I GGNCC 4 cut(s) 139, 221, 371, 395
Cfr9I CCCGGG 1 cut(s) 339
CpoI CGGWCCG 1 cut(s) 395
CseI GACGC 1 cut(s) 295
CspI CGGWCCG 1 cut(s) 395
CviAII CATG 2 cut(s) 287, 299
DpnI GATC 2 cut(s) 64, 303
DpnII GATC 2 cut(s) 62, 301
EaeI YGGCCR 3 cut(s) 75, 159, 348
EagI CGGCCG 2 cut(s) 75, 159
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
EciI GGCGGA 2 cut(s) 72, 408
EclXI CGGCCG 2 cut(s) 75, 159
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 2 cut(s) 371, 395
Eco52I CGGCCG 2 cut(s) 75, 159
Eco88I CYCGRG 2 cut(s) 339, 380
EcoO109I RGGNCCY 1 cut(s) 139
EcoRII CCWGG 3 cut(s) 97, 141, 248
EcoT38I GRGCYC 1 cut(s) 237
Esp3I CGTCTC 2 cut(s) 272, 322
FaeI CATG 2 cut(s) 290, 302
FaiI YATR 4 cut(s) 167, 288, 300, 329
FatI CATG 2 cut(s) 286, 298
FauI CCCGC 1 cut(s) 244
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 9 cut(s) 78, 108, 118, 240, 291, 351, 354, 357, 437
FokI GGATG 3 cut(s) 174, 403, 430
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 9 cut(s) 78, 108, 118, 240, 291, 351, 354, 357, 437
FspBI CTAG 1 cut(s) 203
GlaI GCGC 5 cut(s) 121, 177, 185, 285, 430
GluI GCNGC 9 cut(s) 78, 108, 118, 240, 291, 351, 354, 357, 437
HaeII RGCGCY 1 cut(s) 187
HaeIII GGCC 6 cut(s) 22, 77, 141, 161, 223, 350
HapII CCGG 4 cut(s) 87, 162, 340, 398
HgaI GACGC 1 cut(s) 295
HhaI GCGC 5 cut(s) 122, 178, 186, 286, 431
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 2 cut(s) 290, 302
Hin6I GCGC 5 cut(s) 120, 176, 184, 284, 429
HinP1I GCGC 5 cut(s) 120, 176, 184, 284, 429
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 2 cut(s) 209, 383
HpaII CCGG 4 cut(s) 87, 162, 340, 398
Hpy166II GTNNAC 2 cut(s) 310, 374
Hpy188I TCNGA 1 cut(s) 421
Hpy188III TCNNGA 1 cut(s) 457
Hpy8I GTNNAC 2 cut(s) 310, 374
Hpy99I CGWCG 5 cut(s) 76, 160, 311, 314, 317
HpyAV CCTTC 1 cut(s) 33
HpyCH4III ACNGT 2 cut(s) 371, 410
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 2 cut(s) 68, 107
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 158, 281, 290, 350, 356
HpySE526I ACGT 1 cut(s) 315
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 2 cut(s) 290, 302
HspAI GCGC 5 cut(s) 120, 176, 184, 284, 429
Kzo9I GATC 2 cut(s) 62, 301
LmnI GCTCC 3 cut(s) 85, 232, 252
Lsp1109I GCAGC 3 cut(s) 119, 251, 277
LweI GCATC 2 cut(s) 252, 440
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 4 cut(s) 170, 244, 365, 404
MalI GATC 2 cut(s) 64, 303
MbiI CCGCTC 1 cut(s) 80
MboI GATC 2 cut(s) 62, 301
MboII GAAGA 1 cut(s) 8
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 2 cut(s) 237, 257
MluCI AATT 1 cut(s) 462
MlyI GAGTC 1 cut(s) 392
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 4 cut(s) 24, 135, 419, 464
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 1 cut(s) 260
MspCI CTTAAG 1 cut(s) 179
MspI CCGG 4 cut(s) 87, 162, 340, 398
MspR9I CCNGG 6 cut(s) 87, 99, 143, 250, 340, 341
MvaI CCWGG 3 cut(s) 99, 143, 250
MvnI CGCG 3 cut(s) 120, 176, 306
MwoI GCNNNNNNNGC 6 cut(s) 74, 158, 281, 290, 350, 356
NciI CCSGG 3 cut(s) 87, 340, 341
NdeII GATC 2 cut(s) 62, 301
NlaIII CATG 2 cut(s) 290, 302
NlaIV GGNNCC 3 cut(s) 6, 140, 234
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 2 cut(s) 170, 404
NspI RCATGY 1 cut(s) 290
PaeI GCATGC 1 cut(s) 290
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PfeI GAWTC 1 cut(s) 209
PflFI GACNNNGTC 1 cut(s) 314
PkrI GCNGC 9 cut(s) 79, 109, 119, 241, 292, 352, 355, 358, 438
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
Psp6I CCWGG 3 cut(s) 97, 141, 248
PspGI CCWGG 3 cut(s) 97, 141, 248
PspN4I GGNNCC 3 cut(s) 6, 140, 234
PspPI GGNCC 4 cut(s) 139, 221, 371, 395
PsuI RGATCY 1 cut(s) 62
PsyI GACNNNGTC 1 cut(s) 314
RseI CAYNNNNRTG 1 cut(s) 260
Rsr2I CGGWCCG 1 cut(s) 395
RsrII CGGWCCG 1 cut(s) 395
SalI GTCGAC 1 cut(s) 308
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 9 cut(s) 78, 108, 118, 240, 291, 351, 354, 357, 437
Sau3AI GATC 2 cut(s) 62, 301
Sau96I GGNCC 4 cut(s) 139, 221, 371, 395
SchI GAGTC 1 cut(s) 392
ScrFI CCNGG 6 cut(s) 87, 99, 143, 250, 340, 341
SduI GDGCHC 2 cut(s) 237, 257
SetI ASST 4 cut(s) 10, 103, 172, 318
SfaNI GCATC 2 cut(s) 252, 440
SgrDI CGTCGACG 1 cut(s) 308
SinI GGWCC 2 cut(s) 371, 395
SmaI CCCGGG 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SphI GCATGC 1 cut(s) 290
SrfI GCCCGGGC 1 cut(s) 341
Sse9I AATT 1 cut(s) 462
SspI AATATT 1 cut(s) 44
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 6 cut(s) 85, 97, 141, 248, 338, 339
TaaI ACNGT 2 cut(s) 371, 410
TaiI ACGT 1 cut(s) 318
TaqI TCGA 2 cut(s) 309, 386
TaqII GACCGA 1 cut(s) 438
TasI AATT 1 cut(s) 462
TauI GCSGC 6 cut(s) 80, 120, 353, 356, 359, 439
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TseFI GTSAC 2 cut(s) 170, 404
TseI GCWGC 3 cut(s) 107, 239, 290
Tsp45I GTSAC 2 cut(s) 170, 404
TspGWI ACGGA 1 cut(s) 353
TspMI CCCGGG 1 cut(s) 339
Tth111I GACNNNGTC 1 cut(s) 314
Vha464I CTTAAG 1 cut(s) 179
VpaK11BI GGWCC 2 cut(s) 371, 395
XceI RCATGY 1 cut(s) 290
XmaI CCCGGG 1 cut(s) 339
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 1 cut(s) 203
ZraI GACGTC 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.