Rroxscaffold_15G00447470

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000015
OG0017764
Physical Location & Seq
Forward (+)
4037 .. 4681
645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_15G00447470.1

Sequence Viewer

Length: 456 bp
ATGGGAAGCCCGCAGGCCGTTACCAGGAGCACGCAGATGCCGAAGCACGCCGAGACGGCGCATGCTGCCTACCATGATCGCGTCGACGACGTCTCCACGGGCATATCAACAGCCGGGCTTTGGCCGCCGCCGCAATCCGTAACGGTCCACGCCCGAGTCGATCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGGTGCGACAGGGGTCCGGGCACAACGGGGCTCTCACCCTCTATGGCGCCCCCTTCCAGGGGACTTGTTCCGGTCCGCCTCTGAGGACGCTTCTCCAGACTACAATTCGGACGCCTTACGACGCCAGATTCTCAAGCTGGGCTATTCCCGGTTCGCTCGCCGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

16.58

Weight (kDa)

9.82

Isoelectric Point (pI)

55.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 333
AccI GTMKAC 1 cut(s) 84
AccII CGCG 2 cut(s) 81, 182
AciI CCGC 7 cut(s) 11, 125, 128, 131, 182, 236, 363
AclWI GGATC 1 cut(s) 155
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 1 cut(s) 229
AcyI GRCGYC 4 cut(s) 90, 334, 399, 409
AfaI GTAC 1 cut(s) 186
AfiI CCNNNNNNNGG 6 cut(s) 24, 120, 245, 344, 345, 346
AjnI CCWGG 2 cut(s) 23, 343
AluBI AGCT 1 cut(s) 424
AluI AGCT 1 cut(s) 424
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 3 cut(s) 47, 97, 207
AlwI GGATC 1 cut(s) 155
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 2 cut(s) 15, 122
ApeKI GCWGC 2 cut(s) 65, 249
ApoI RAATTY 1 cut(s) 229
AspLEI GCGC 3 cut(s) 61, 283, 336
AspS9I GGNCC 3 cut(s) 145, 301, 360
AsuC2I CCSGG 3 cut(s) 115, 305, 436
AsuHPI GGTGA 1 cut(s) 314
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 3 cut(s) 145, 301, 360
BaeGI GKGCMC 1 cut(s) 310
BanI GGYRCC 1 cut(s) 333
BanII GRGCYC 1 cut(s) 320
BauI CACGAG 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 52, 236
BceAI ACGGC 4 cut(s) 2, 72, 194, 432
BciT130I CCWGG 2 cut(s) 25, 345
BcnI CCSGG 3 cut(s) 115, 305, 436
BcoDI GTCTC 3 cut(s) 47, 97, 207
BfoI RGCGCY 2 cut(s) 284, 337
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 5 cut(s) 66, 125, 128, 131, 250
BlsI GCNGC 5 cut(s) 67, 126, 129, 132, 251
Bme1390I CCNGG 5 cut(s) 25, 115, 305, 345, 436
Bme18I GGWCC 3 cut(s) 145, 301, 360
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 145, 301, 360
BmiI GGNNCC 2 cut(s) 302, 335
BmrFI CCNGG 5 cut(s) 25, 115, 305, 345, 436
BmsI GCATC 1 cut(s) 27
BoxI GACNNNNGTC 1 cut(s) 299
BpmI CTGGAG 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 3 cut(s) 115, 305, 436
BsaHI GRCGYC 4 cut(s) 90, 334, 399, 409
BsaI GGTCTC 1 cut(s) 207
BsaJI CCNNGG 3 cut(s) 96, 220, 344
BsaWI WCCGGW 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 6 cut(s) 24, 120, 245, 344, 345, 346
BseBI CCWGG 2 cut(s) 25, 345
BseDI CCNNGG 3 cut(s) 96, 220, 344
BseLI CCNNNNNNNGG 6 cut(s) 24, 120, 245, 344, 345, 346
BseMII CTCAG 1 cut(s) 360
BseSI GKGCMC 1 cut(s) 310
BseXI GCAGC 2 cut(s) 52, 236
BseYI CCCAGC 1 cut(s) 424
Bsh1236I CGCG 2 cut(s) 81, 182
BshFI GGCC 2 cut(s) 17, 124
BshNI GGYRCC 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 4 cut(s) 114, 304, 358, 436
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 6 cut(s) 24, 120, 245, 344, 345, 346
BsmAI GTCTC 3 cut(s) 47, 97, 207
BsmBI CGTCTC 2 cut(s) 47, 97
BsmFI GGGAC 1 cut(s) 362
BsmI GAATGC 1 cut(s) 252
BsnI GGCC 2 cut(s) 17, 124
Bso31I GGTCTC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 153
Bsp1286I GDGCHC 3 cut(s) 32, 310, 320
Bsp143I GATC 2 cut(s) 76, 160
BspACI CCGC 7 cut(s) 11, 125, 128, 131, 182, 236, 363
BspANI GGCC 2 cut(s) 17, 124
BspCNI CTCAG 1 cut(s) 361
BspFNI CGCG 2 cut(s) 81, 182
BspLI GGNNCC 2 cut(s) 302, 335
BspPI GGATC 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 333
BspTNI GGTCTC 1 cut(s) 207
BssECI CCNNGG 3 cut(s) 96, 220, 344
BssMI GATC 2 cut(s) 76, 160
BssNI GRCGYC 4 cut(s) 90, 334, 399, 409
BssSI CACGAG 1 cut(s) 285
BssT1I CCWWGG 1 cut(s) 220
Bst2BI CACGAG 1 cut(s) 285
Bst2UI CCWGG 2 cut(s) 25, 345
Bst4CI ACNGT 1 cut(s) 145
BstACI GRCGYC 4 cut(s) 90, 334, 399, 409
BstC8I GCNNGC 6 cut(s) 11, 15, 32, 48, 63, 445
BstDEI CTNAG 1 cut(s) 369
BstDSI CCRYGG 1 cut(s) 96
BstFNI CGCG 2 cut(s) 81, 182
BstH2I RGCGCY 2 cut(s) 284, 337
BstHHI GCGC 3 cut(s) 61, 283, 336
BstKTI GATC 2 cut(s) 79, 163
BstMAI GTCTC 3 cut(s) 47, 97, 207
BstMBI GATC 2 cut(s) 76, 160
BstMWI GCNNNNNNNGC 5 cut(s) 56, 65, 124, 130, 280
BstNI CCWGG 2 cut(s) 25, 345
BstNSI RCATGY 1 cut(s) 65
BstPAI GACNNNNGTC 1 cut(s) 299
BstSCI CCNGG 5 cut(s) 23, 113, 303, 343, 434
BstSLI GKGCMC 1 cut(s) 310
BstUI CGCG 2 cut(s) 81, 182
BstV1I GCAGC 2 cut(s) 52, 236
BsuRI GGCC 2 cut(s) 17, 124
BtgI CCRYGG 1 cut(s) 96
Cac8I GCNNGC 6 cut(s) 11, 15, 32, 48, 63, 445
CfoI GCGC 3 cut(s) 61, 283, 336
Cfr13I GGNCC 3 cut(s) 145, 301, 360
CpoI CGGWCCG 1 cut(s) 360
CseI GACGC 4 cut(s) 70, 383, 407, 417
Csp6I GTAC 1 cut(s) 185
CspI CGGWCCG 1 cut(s) 360
CviAII CATG 2 cut(s) 62, 74
CviQI GTAC 1 cut(s) 185
DdeI CTNAG 1 cut(s) 369
DinI GGCGCC 1 cut(s) 335
DpnI GATC 2 cut(s) 78, 162
DpnII GATC 2 cut(s) 76, 160
EaeI YGGCCR 1 cut(s) 122
EciI GGCGGA 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 320
Eco31I GGTCTC 1 cut(s) 207
Eco47I GGWCC 3 cut(s) 145, 301, 360
Eco88I CYCGRG 1 cut(s) 153
EcoRII CCWGG 2 cut(s) 23, 343
EcoT14I CCWWGG 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 320
EgeI GGCGCC 1 cut(s) 335
EheI GGCGCC 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 220
Esp3I CGTCTC 2 cut(s) 47, 97
FaeI CATG 2 cut(s) 65, 77
FaiI YATR 4 cut(s) 63, 75, 104, 331
FaqI GGGAC 1 cut(s) 362
FatI CATG 2 cut(s) 61, 73
FauI CCCGC 1 cut(s) 18
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 5 cut(s) 66, 125, 128, 131, 250
FriOI GRGCYC 1 cut(s) 320
Fsp4HI GCNGC 5 cut(s) 66, 125, 128, 131, 250
GlaI GCGC 3 cut(s) 60, 282, 335
GluI GCNGC 5 cut(s) 66, 125, 128, 131, 250
GsaI CCCAGC 1 cut(s) 428
GsuI CTGGAG 1 cut(s) 366
HaeII RGCGCY 2 cut(s) 284, 337
HaeIII GGCC 2 cut(s) 17, 124
HapII CCGG 4 cut(s) 114, 304, 358, 436
HgaI GACGC 4 cut(s) 70, 383, 407, 417
HhaI GCGC 3 cut(s) 61, 283, 336
Hin1I GRCGYC 4 cut(s) 90, 334, 399, 409
Hin1II CATG 2 cut(s) 65, 77
Hin6I GCGC 3 cut(s) 59, 281, 334
HinP1I GCGC 3 cut(s) 59, 281, 334
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 3 cut(s) 156, 269, 415
HpaII CCGG 4 cut(s) 114, 304, 358, 436
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 2 cut(s) 85, 148
Hpy188I TCNGA 2 cut(s) 370, 397
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 2 cut(s) 85, 148
Hpy99I CGWCG 4 cut(s) 86, 89, 92, 411
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 1 cut(s) 252
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 65, 124, 130, 280
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 4 cut(s) 90, 334, 399, 409
Hsp92II CATG 2 cut(s) 65, 77
HspAI GCGC 3 cut(s) 59, 281, 334
KasI GGCGCC 1 cut(s) 333
Kzo9I GATC 2 cut(s) 76, 160
LmnI GCTCC 1 cut(s) 27
Lsp1109I GCAGC 2 cut(s) 52, 236
LweI GCATC 1 cut(s) 27
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 3 cut(s) 19, 139, 449
MalI GATC 2 cut(s) 78, 162
MboI GATC 2 cut(s) 76, 160
MhlI GDGCHC 3 cut(s) 32, 310, 320
MluCI AATT 2 cut(s) 229, 391
Mly113I GGCGCC 1 cut(s) 334
MlyI GAGTC 2 cut(s) 165, 263
MnlI CCTC 5 cut(s) 188, 294, 336, 364, 376
MslI CAYNNNNRTG 1 cut(s) 35
MspI CCGG 4 cut(s) 114, 304, 358, 436
MspR9I CCNGG 5 cut(s) 25, 115, 305, 345, 436
Mva1269I GAATGC 1 cut(s) 252
MvaI CCWGG 2 cut(s) 25, 345
MvnI CGCG 2 cut(s) 81, 182
MwoI GCNNNNNNNGC 5 cut(s) 56, 65, 124, 130, 280
NarI GGCGCC 1 cut(s) 334
NciI CCSGG 3 cut(s) 115, 305, 436
NdeII GATC 2 cut(s) 76, 160
NlaIII CATG 2 cut(s) 65, 77
NlaIV GGNNCC 2 cut(s) 302, 335
NmeAIII GCCGAG 1 cut(s) 76
NspI RCATGY 1 cut(s) 65
PaeI GCATGC 1 cut(s) 65
PcsI WCGNNNNNNNCGW 2 cut(s) 38, 156
PctI GAATGC 1 cut(s) 252
PfeI GAWTC 1 cut(s) 415
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 5 cut(s) 67, 126, 129, 132, 251
PleI GAGTC 2 cut(s) 164, 263
PluTI GGCGCC 1 cut(s) 337
PpsI GAGTC 2 cut(s) 164, 263
PshAI GACNNNNGTC 1 cut(s) 299
Psp6I CCWGG 2 cut(s) 23, 343
PspFI CCCAGC 1 cut(s) 424
PspGI CCWGG 2 cut(s) 23, 343
PspN4I GGNNCC 2 cut(s) 302, 335
PspPI GGNCC 3 cut(s) 145, 301, 360
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
RseI CAYNNNNRTG 1 cut(s) 35
Rsr2I CGGWCCG 1 cut(s) 360
RsrII CGGWCCG 1 cut(s) 360
SalI GTCGAC 1 cut(s) 83
SatI GCNGC 5 cut(s) 66, 125, 128, 131, 250
Sau3AI GATC 2 cut(s) 76, 160
Sau96I GGNCC 3 cut(s) 145, 301, 360
SchI GAGTC 2 cut(s) 165, 263
ScrFI CCNGG 5 cut(s) 25, 115, 305, 345, 436
SduI GDGCHC 3 cut(s) 32, 310, 320
SetI ASST 2 cut(s) 93, 426
SfaNI GCATC 1 cut(s) 27
SfoI GGCGCC 1 cut(s) 335
SgrDI CGTCGACG 1 cut(s) 83
SinI GGWCC 3 cut(s) 145, 301, 360
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
SphI GCATGC 1 cut(s) 65
Sse9I AATT 2 cut(s) 229, 391
SsiI CCGC 7 cut(s) 11, 125, 128, 131, 182, 236, 363
SspDI GGCGCC 1 cut(s) 333
StyD4I CCNGG 5 cut(s) 23, 113, 303, 343, 434
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 1 cut(s) 93
TaqI TCGA 2 cut(s) 84, 159
TaqII GACCGA 1 cut(s) 189
TasI AATT 2 cut(s) 229, 391
TauI GCSGC 3 cut(s) 127, 130, 133
TfiI GAWTC 1 cut(s) 415
TseI GCWGC 2 cut(s) 65, 249
TspDTI ATGAA 1 cut(s) 158
TspGWI ACGGA 2 cut(s) 127, 241
Tth111I GACNNNGTC 1 cut(s) 89
VpaK11BI GGWCC 3 cut(s) 145, 301, 360
XapI RAATTY 1 cut(s) 229
XceI RCATGY 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 84
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.