Rroxscaffold_16G00446270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000016
OG0017764
Physical Location & Seq
Forward (+)
83915 .. 85145
1231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_16G00446270.1

Sequence Viewer

Length: 651 bp
ATGTCGGACTCCTTGGTCGTGTTTCAAGACGGGCCGAATGGGGAGCGCGCGGCCGTTACCGGGAGCACGCAGATGCCGAAGCACGCCGAGACGGCGCGTGCTGCCTACCATGATCGCGTCGACGACGTCTCCACGGGCATATCAACAGCCGGGCTTTGGCCGCCGCCGCGATCCGTAACGGTCCACGCCCGAGTCGACCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACGGCGCCTCGTGGTGCGACAGGGTCCGGGCACAACGGGGCTCTCACCCTCTATGGCGCCCCCTTCCGGGGGACTTGTTCCCGGTCCCGCCTGCGAGGACGCTTCTCCGGACTACAATTCGGACGCCTTACGACGCCGGATTCTCAAAGCTGGGCTATTCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTAGCCGAGATATCCGTTGCCGAGAGTCGTTTAGACATATTGAAAGACGACGAAACCACCCGCACGATCACCGTCTCCGGGACGGCGGGGAACGCTCTTTCATTCAAGTTCCTTGGCGCAATTCACGCCGGTGTTCAGTACGCCGGGAGGGTCGATCAAGCGCAAGCACCGAAATGCAACACGCAAAGACACTTGCCTCCCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

216

Amino Acids

23.02

Weight (kDa)

9.45

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
AatII GACGTC 1 cut(s) 129
AccB1I GGYRCC 2 cut(s) 259, 311
AccI GTMKAC 2 cut(s) 120, 195
AccII CGCG 5 cut(s) 48, 50, 97, 117, 169
AccIII TCCGGA 1 cut(s) 362
AciI CCGC 8 cut(s) 50, 161, 164, 167, 215, 343, 505, 530
AclWI GGATC 1 cut(s) 165
AcoI YGGCCR 2 cut(s) 51, 158
AcsI RAATTY 1 cut(s) 208
AcyI GRCGYC 5 cut(s) 126, 260, 312, 379, 389
AfaI GTAC 1 cut(s) 584
AfiI CCNNNNNNNGG 8 cut(s) 60, 156, 224, 321, 322, 323, 324, 522
AgsI TTSAA 3 cut(s) 26, 487, 550
AleI CACNNNNGTG 1 cut(s) 573
AluBI AGCT 1 cut(s) 405
AluI AGCT 1 cut(s) 405
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 3 cut(s) 83, 133, 523
AlwI GGATC 1 cut(s) 165
Ama87I CYCGRG 1 cut(s) 189
Aor13HI TCCGGA 1 cut(s) 362
AoxI GGCC 3 cut(s) 32, 51, 158
ApeKI GCWGC 2 cut(s) 101, 228
ApoI RAATTY 1 cut(s) 208
AspLEI GCGC 7 cut(s) 48, 50, 97, 262, 314, 563, 607
AspS9I GGNCC 4 cut(s) 32, 181, 279, 339
AsuC2I CCSGG 7 cut(s) 61, 151, 283, 323, 337, 523, 589
AsuHPI GGTGA 2 cut(s) 292, 505
AvaI CYCGRG 1 cut(s) 189
AvaII GGWCC 3 cut(s) 181, 279, 339
BaeGI GKGCMC 1 cut(s) 288
BanI GGYRCC 2 cut(s) 259, 311
BanII GRGCYC 1 cut(s) 298
BauI CACGAG 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 68
BbvI GCAGC 2 cut(s) 88, 215
BceAI ACGGC 5 cut(s) 38, 108, 273, 412, 543
BcnI CCSGG 7 cut(s) 61, 151, 283, 323, 337, 523, 589
BcoDI GTCTC 3 cut(s) 83, 133, 523
BfaI CTAG 1 cut(s) 649
BfoI RGCGCY 2 cut(s) 263, 315
BglI GCCNNNNNGGC 1 cut(s) 92
BisI GCNGC 6 cut(s) 51, 102, 161, 164, 167, 229
BlsI GCNGC 6 cut(s) 52, 103, 162, 165, 168, 230
Bme1390I CCNGG 7 cut(s) 61, 151, 283, 323, 337, 523, 589
Bme18I GGWCC 3 cut(s) 181, 279, 339
BmeT110I CYCGRG 1 cut(s) 189
BmgT120I GGNCC 4 cut(s) 32, 181, 279, 339
BmiI GGNNCC 4 cut(s) 261, 280, 313, 341
BmrFI CCNGG 7 cut(s) 61, 151, 283, 323, 337, 523, 589
BmsI GCATC 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 445
BpuMI CCSGG 7 cut(s) 61, 151, 283, 323, 337, 523, 589
BsaHI GRCGYC 5 cut(s) 126, 260, 312, 379, 389
BsaJI CCNNGG 5 cut(s) 12, 132, 199, 322, 556
BsaWI WCCGGW 2 cut(s) 362, 415
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 8 cut(s) 60, 156, 224, 321, 322, 323, 324, 522
Bse118I RCCGGY 1 cut(s) 572
BseAI TCCGGA 1 cut(s) 362
BseDI CCNNGG 5 cut(s) 12, 132, 199, 322, 556
BseLI CCNNNNNNNGG 8 cut(s) 60, 156, 224, 321, 322, 323, 324, 522
BsePI GCGCGC 1 cut(s) 46
BseSI GKGCMC 1 cut(s) 288
BseX3I CGGCCG 1 cut(s) 51
BseXI GCAGC 2 cut(s) 88, 215
BseYI CCCAGC 1 cut(s) 405
Bsh1236I CGCG 5 cut(s) 48, 50, 97, 117, 169
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 3 cut(s) 34, 53, 160
BshNI GGYRCC 2 cut(s) 259, 311
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 68
BsiHKCI CYCGRG 1 cut(s) 189
BslFI GGGAC 3 cut(s) 325, 340, 538
BslI CCNNNNNNNGG 8 cut(s) 60, 156, 224, 321, 322, 323, 324, 522
BsmAI GTCTC 3 cut(s) 83, 133, 523
BsmBI CGTCTC 3 cut(s) 83, 133, 523
BsmFI GGGAC 3 cut(s) 325, 340, 538
BsmI GAATGC 1 cut(s) 231
BsnI GGCC 3 cut(s) 34, 53, 160
BsoBI CYCGRG 1 cut(s) 189
Bsp1286I GDGCHC 3 cut(s) 68, 288, 298
Bsp13I TCCGGA 1 cut(s) 362
Bsp143I GATC 4 cut(s) 112, 170, 510, 598
BspACI CCGC 8 cut(s) 50, 161, 164, 167, 215, 343, 505, 530
BspANI GGCC 3 cut(s) 34, 53, 160
BspEI TCCGGA 1 cut(s) 362
BspFNI CGCG 5 cut(s) 48, 50, 97, 117, 169
BspLI GGNNCC 4 cut(s) 261, 280, 313, 341
BspPI GGATC 1 cut(s) 165
BspT107I GGYRCC 2 cut(s) 259, 311
BsrFI RCCGGY 1 cut(s) 572
BssAI RCCGGY 1 cut(s) 572
BssECI CCNNGG 5 cut(s) 12, 132, 199, 322, 556
BssHII GCGCGC 1 cut(s) 46
BssMI GATC 4 cut(s) 112, 170, 510, 598
BssNI GRCGYC 5 cut(s) 126, 260, 312, 379, 389
BssSI CACGAG 1 cut(s) 264
BssT1I CCWWGG 3 cut(s) 12, 199, 556
Bst2BI CACGAG 1 cut(s) 264
Bst4CI ACNGT 2 cut(s) 181, 517
BstACI GRCGYC 5 cut(s) 126, 260, 312, 379, 389
BstC8I GCNNGC 7 cut(s) 48, 68, 84, 99, 347, 425, 609
BstDEI CTNAG 2 cut(s) 434, 445
BstDSI CCRYGG 1 cut(s) 132
BstFNI CGCG 5 cut(s) 48, 50, 97, 117, 169
BstH2I RGCGCY 2 cut(s) 263, 315
BstHHI GCGC 7 cut(s) 48, 50, 97, 262, 314, 563, 607
BstKTI GATC 4 cut(s) 115, 173, 513, 601
BstMAI GTCTC 3 cut(s) 83, 133, 523
BstMBI GATC 4 cut(s) 112, 170, 510, 598
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 7 cut(s) 92, 101, 160, 166, 259, 536, 569
BstSCI CCNGG 7 cut(s) 59, 149, 281, 321, 335, 521, 587
BstSLI GKGCMC 1 cut(s) 288
BstUI CGCG 5 cut(s) 48, 50, 97, 117, 169
BstV1I GCAGC 2 cut(s) 88, 215
BstZI CGGCCG 1 cut(s) 51
BsuRI GGCC 3 cut(s) 34, 53, 160
BtgI CCRYGG 1 cut(s) 132
Cac8I GCNNGC 7 cut(s) 48, 68, 84, 99, 347, 425, 609
CfoI GCGC 7 cut(s) 48, 50, 97, 262, 314, 563, 607
Cfr10I RCCGGY 1 cut(s) 572
Cfr13I GGNCC 4 cut(s) 32, 181, 279, 339
CseI GACGC 4 cut(s) 106, 363, 387, 397
Csp6I GTAC 1 cut(s) 583
CviAII CATG 1 cut(s) 110
CviQI GTAC 1 cut(s) 583
DdeI CTNAG 2 cut(s) 434, 445
DinI GGCGCC 2 cut(s) 261, 313
DpnI GATC 4 cut(s) 114, 172, 512, 600
DpnII GATC 4 cut(s) 112, 170, 510, 598
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
EaeI YGGCCR 2 cut(s) 51, 158
EagI CGGCCG 1 cut(s) 51
EclXI CGGCCG 1 cut(s) 51
Eco130I CCWWGG 3 cut(s) 12, 199, 556
Eco24I GRGCYC 1 cut(s) 298
Eco32I GATATC 1 cut(s) 456
Eco47I GGWCC 3 cut(s) 181, 279, 339
Eco52I CGGCCG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 189
EcoRV GATATC 1 cut(s) 456
EcoT14I CCWWGG 3 cut(s) 12, 199, 556
EcoT38I GRGCYC 1 cut(s) 298
EgeI GGCGCC 2 cut(s) 261, 313
EheI GGCGCC 2 cut(s) 261, 313
ErhI CCWWGG 3 cut(s) 12, 199, 556
Esp3I CGTCTC 3 cut(s) 83, 133, 523
FaeI CATG 1 cut(s) 113
FaiI YATR 4 cut(s) 111, 140, 309, 482
FaqI GGGAC 3 cut(s) 325, 340, 538
FatI CATG 1 cut(s) 109
FauI CCCGC 3 cut(s) 350, 512, 523
FblI GTMKAC 2 cut(s) 120, 195
Fnu4HI GCNGC 6 cut(s) 51, 102, 161, 164, 167, 229
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 6 cut(s) 51, 102, 161, 164, 167, 229
FspBI CTAG 1 cut(s) 649
GlaI GCGC 7 cut(s) 47, 49, 96, 261, 313, 562, 606
GluI GCNGC 6 cut(s) 51, 102, 161, 164, 167, 229
GsaI CCCAGC 1 cut(s) 409
HaeII RGCGCY 2 cut(s) 263, 315
HaeIII GGCC 3 cut(s) 34, 53, 160
HgaI GACGC 4 cut(s) 106, 363, 387, 397
HhaI GCGC 7 cut(s) 48, 50, 97, 262, 314, 563, 607
Hin1I GRCGYC 5 cut(s) 126, 260, 312, 379, 389
Hin1II CATG 1 cut(s) 113
Hin6I GCGC 7 cut(s) 46, 48, 95, 260, 312, 561, 605
HinP1I GCGC 7 cut(s) 46, 48, 95, 260, 312, 561, 605
HincII GTYRAC 2 cut(s) 121, 196
HindII GTYRAC 2 cut(s) 121, 196
HinfI GANTC 6 cut(s) 8, 192, 248, 395, 440, 469
HphI GGTGA 2 cut(s) 292, 505
Hpy166II GTNNAC 3 cut(s) 121, 184, 196
Hpy188I TCNGA 2 cut(s) 7, 377
Hpy188III TCNNGA 2 cut(s) 26, 363
Hpy8I GTNNAC 3 cut(s) 121, 184, 196
Hpy99I CGWCG 6 cut(s) 122, 125, 128, 259, 391, 497
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 2 cut(s) 181, 517
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 2 cut(s) 231, 621
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 101, 160, 166, 259, 536, 569
HpyF3I CTNAG 2 cut(s) 434, 445
HpySE526I ACGT 1 cut(s) 126
Hsp92I GRCGYC 5 cut(s) 126, 260, 312, 379, 389
Hsp92II CATG 1 cut(s) 113
HspAI GCGC 7 cut(s) 46, 48, 95, 260, 312, 561, 605
KasI GGCGCC 2 cut(s) 259, 311
Kpn2I TCCGGA 1 cut(s) 362
Kzo9I GATC 4 cut(s) 112, 170, 510, 598
LmnI GCTCC 2 cut(s) 43, 63
Lsp1109I GCAGC 2 cut(s) 88, 215
LweI GCATC 1 cut(s) 63
MaeI CTAG 1 cut(s) 649
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 3 cut(s) 55, 175, 429
MalI GATC 4 cut(s) 114, 172, 512, 600
MboI GATC 4 cut(s) 112, 170, 510, 598
MhlI GDGCHC 3 cut(s) 68, 288, 298
MluCI AATT 3 cut(s) 208, 371, 564
Mly113I GGCGCC 2 cut(s) 260, 312
MlyI GAGTC 4 cut(s) 2, 201, 242, 478
MnlI CCTC 5 cut(s) 273, 314, 344, 585, 651
MroI TCCGGA 1 cut(s) 362
MslI CAYNNNNRTG 3 cut(s) 71, 573, 616
MspR9I CCNGG 7 cut(s) 61, 151, 283, 323, 337, 523, 589
Mva1269I GAATGC 1 cut(s) 231
MvnI CGCG 5 cut(s) 48, 50, 97, 117, 169
MwoI GCNNNNNNNGC 7 cut(s) 92, 101, 160, 166, 259, 536, 569
NarI GGCGCC 2 cut(s) 260, 312
NciI CCSGG 7 cut(s) 61, 151, 283, 323, 337, 523, 589
NdeII GATC 4 cut(s) 112, 170, 510, 598
NlaIII CATG 1 cut(s) 113
NlaIV GGNNCC 4 cut(s) 261, 280, 313, 341
NmeAIII GCCGAG 3 cut(s) 112, 475, 490
OliI CACNNNNGTG 1 cut(s) 573
PauI GCGCGC 1 cut(s) 46
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 192
PctI GAATGC 1 cut(s) 231
PfeI GAWTC 2 cut(s) 395, 440
PflFI GACNNNGTC 2 cut(s) 125, 277
PfoI TCCNGGA 1 cut(s) 521
PkrI GCNGC 6 cut(s) 52, 103, 162, 165, 168, 230
PleI GAGTC 4 cut(s) 2, 200, 242, 477
PluTI GGCGCC 2 cut(s) 263, 315
PpsI GAGTC 4 cut(s) 2, 200, 242, 477
PspFI CCCAGC 1 cut(s) 405
PspN4I GGNNCC 4 cut(s) 261, 280, 313, 341
PspPI GGNCC 4 cut(s) 32, 181, 279, 339
PsyI GACNNNGTC 2 cut(s) 125, 277
PteI GCGCGC 1 cut(s) 46
RsaI GTAC 1 cut(s) 584
RsaNI GTAC 1 cut(s) 583
RseI CAYNNNNRTG 3 cut(s) 71, 573, 616
SalI GTCGAC 2 cut(s) 119, 194
SatI GCNGC 6 cut(s) 51, 102, 161, 164, 167, 229
Sau3AI GATC 4 cut(s) 112, 170, 510, 598
Sau96I GGNCC 4 cut(s) 32, 181, 279, 339
SchI GAGTC 4 cut(s) 2, 201, 242, 478
ScrFI CCNGG 7 cut(s) 61, 151, 283, 323, 337, 523, 589
SduI GDGCHC 3 cut(s) 68, 288, 298
SetI ASST 3 cut(s) 129, 201, 407
SfaNI GCATC 1 cut(s) 63
SfoI GGCGCC 2 cut(s) 261, 313
SgrAI CRCCGGYG 1 cut(s) 572
SgrDI CGTCGACG 1 cut(s) 119
SinI GGWCC 3 cut(s) 181, 279, 339
SmiMI CAYNNNNRTG 3 cut(s) 71, 573, 616
Sse9I AATT 3 cut(s) 208, 371, 564
SsiI CCGC 8 cut(s) 50, 161, 164, 167, 215, 343, 505, 530
SspDI GGCGCC 2 cut(s) 259, 311
SspMI CTAG 1 cut(s) 649
StyD4I CCNGG 7 cut(s) 59, 149, 281, 321, 335, 521, 587
StyI CCWWGG 3 cut(s) 12, 199, 556
TaaI ACNGT 2 cut(s) 181, 517
TaiI ACGT 1 cut(s) 129
TaqI TCGA 3 cut(s) 120, 195, 597
TasI AATT 3 cut(s) 208, 371, 564
TauI GCSGC 4 cut(s) 53, 163, 166, 169
TfiI GAWTC 2 cut(s) 395, 440
TseI GCWGC 2 cut(s) 101, 228
TspDTI ATGAA 1 cut(s) 534
TspGWI ACGGA 3 cut(s) 163, 220, 448
Tth111I GACNNNGTC 2 cut(s) 125, 277
VpaK11BI GGWCC 3 cut(s) 181, 279, 339
XapI RAATTY 1 cut(s) 208
XmiI GTMKAC 2 cut(s) 120, 195
XspI CTAG 1 cut(s) 649
ZraI GACGTC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.