Rroxscaffold_68G00446980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000068
OG0017764
Physical Location & Seq
Forward (+)
5445 .. 6079
635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_68G00446980.1

Sequence Viewer

Length: 528 bp
ATGTCGGACTCCTTGGTCCGTGTTTCAAGACGGGCCGAATGGGAAGCCCGCGGCCGTTACCGGGAGCACGCAGATGCCGAAGCACGCCGAGACGGCGCGTGCGCCTACCATGATCGCGTCGACGACGTCTCCACGGGCATATCAACAGCCGGGCTTTGGCCGCCGCCGCAATCCGTAACGGTCCACGCCCGAGTCGATCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACGGCGCCTCGTGGTGCGACGGGGTCCGGGCACAACGGGGCTCTCACCCTCTATGGCGCCCCCTTCCGGGGGACTTGTTCCCGGTCCGCCTGCGAGGACGCTTCTCCGGACTACAATTCGGACGCCTTACGACGCCGGATTCTCAGGCGGGCTATTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTAAGTTTCTTTTCCTCCGCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

18.99

Weight (kDa)

9.16

Isoelectric Point (pI)

56.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
AatII GACGTC 1 cut(s) 129
AccB1I GGYRCC 2 cut(s) 316, 368
AccI GTMKAC 1 cut(s) 120
AccII CGCG 4 cut(s) 51, 98, 117, 218
AccIII TCCGGA 1 cut(s) 418
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 2 cut(s) 52, 158
AcsI RAATTY 1 cut(s) 265
AcyI GRCGYC 5 cut(s) 126, 317, 369, 435, 445
AfaI GTAC 1 cut(s) 222
AfiI CCNNNNNNNGG 7 cut(s) 61, 156, 281, 378, 379, 380, 381
AgsI TTSAA 1 cut(s) 27
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 3 cut(s) 84, 133, 243
AlwI GGATC 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 189
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 3 cut(s) 33, 52, 158
ApeKI GCWGC 1 cut(s) 285
ApoI RAATTY 1 cut(s) 265
AspLEI GCGC 4 cut(s) 98, 104, 319, 371
AspS9I GGNCC 5 cut(s) 16, 33, 181, 336, 396
AsuC2I CCSGG 6 cut(s) 62, 151, 340, 380, 394, 471
AsuHPI GGTGA 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 189
AvaII GGWCC 4 cut(s) 16, 181, 336, 396
BaeGI GKGCMC 1 cut(s) 345
BanI GGYRCC 2 cut(s) 316, 368
BanII GRGCYC 1 cut(s) 355
BauI CACGAG 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 272
BceAI ACGGC 5 cut(s) 39, 109, 230, 330, 467
BcnI CCSGG 6 cut(s) 62, 151, 340, 380, 394, 471
BcoDI GTCTC 3 cut(s) 84, 133, 243
BfoI RGCGCY 2 cut(s) 320, 372
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 5 cut(s) 52, 161, 164, 167, 286
BlsI GCNGC 5 cut(s) 53, 162, 165, 168, 287
Bme1390I CCNGG 6 cut(s) 62, 151, 340, 380, 394, 471
Bme18I GGWCC 4 cut(s) 16, 181, 336, 396
BmeT110I CYCGRG 1 cut(s) 189
BmgT120I GGNCC 5 cut(s) 16, 33, 181, 336, 396
BmiI GGNNCC 3 cut(s) 318, 337, 370
BmrFI CCNGG 6 cut(s) 62, 151, 340, 380, 394, 471
BmsI GCATC 1 cut(s) 64
BpuMI CCSGG 6 cut(s) 62, 151, 340, 380, 394, 471
BsaHI GRCGYC 5 cut(s) 126, 317, 369, 435, 445
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 5 cut(s) 12, 49, 132, 256, 379
BsaWI WCCGGW 1 cut(s) 418
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 7 cut(s) 61, 156, 281, 378, 379, 380, 381
BseAI TCCGGA 1 cut(s) 418
BseDI CCNNGG 5 cut(s) 12, 49, 132, 256, 379
BseLI CCNNNNNNNGG 7 cut(s) 61, 156, 281, 378, 379, 380, 381
BseMII CTCAG 1 cut(s) 469
BseSI GKGCMC 1 cut(s) 345
BseX3I CGGCCG 1 cut(s) 52
BseXI GCAGC 1 cut(s) 272
Bsh1236I CGCG 4 cut(s) 51, 98, 117, 218
Bsh1285I CGRYCG 1 cut(s) 55
BshFI GGCC 3 cut(s) 35, 54, 160
BshNI GGYRCC 2 cut(s) 316, 368
BsiEI CGRYCG 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 69
BsiHKCI CYCGRG 1 cut(s) 189
BsiSI CCGG 8 cut(s) 61, 150, 339, 379, 394, 419, 448, 471
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 7 cut(s) 61, 156, 281, 378, 379, 380, 381
BsmAI GTCTC 3 cut(s) 84, 133, 243
BsmBI CGTCTC 2 cut(s) 84, 133
BsmFI GGGAC 1 cut(s) 397
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 3 cut(s) 35, 54, 160
Bso31I GGTCTC 1 cut(s) 243
BsoBI CYCGRG 1 cut(s) 189
Bsp1286I GDGCHC 3 cut(s) 69, 345, 355
Bsp13I TCCGGA 1 cut(s) 418
Bsp143I GATC 2 cut(s) 112, 196
BspANI GGCC 3 cut(s) 35, 54, 160
BspCNI CTCAG 1 cut(s) 468
BspEI TCCGGA 1 cut(s) 418
BspFNI CGCG 4 cut(s) 51, 98, 117, 218
BspLI GGNNCC 3 cut(s) 318, 337, 370
BspPI GGATC 1 cut(s) 191
BspT107I GGYRCC 2 cut(s) 316, 368
BspTNI GGTCTC 1 cut(s) 243
BssECI CCNNGG 5 cut(s) 12, 49, 132, 256, 379
BssMI GATC 2 cut(s) 112, 196
BssNI GRCGYC 5 cut(s) 126, 317, 369, 435, 445
BssSI CACGAG 1 cut(s) 321
BssT1I CCWWGG 2 cut(s) 12, 256
Bst2BI CACGAG 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 181
BstACI GRCGYC 5 cut(s) 126, 317, 369, 435, 445
BstC8I GCNNGC 7 cut(s) 49, 69, 85, 100, 403, 462, 480
BstDEI CTNAG 2 cut(s) 455, 489
BstDSI CCRYGG 2 cut(s) 49, 132
BstFNI CGCG 4 cut(s) 51, 98, 117, 218
BstH2I RGCGCY 2 cut(s) 320, 372
BstHHI GCGC 4 cut(s) 98, 104, 319, 371
BstKTI GATC 2 cut(s) 115, 199
BstMAI GTCTC 3 cut(s) 84, 133, 243
BstMBI GATC 2 cut(s) 112, 196
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 4 cut(s) 93, 160, 166, 316
BstSCI CCNGG 6 cut(s) 60, 149, 338, 378, 392, 469
BstSLI GKGCMC 1 cut(s) 345
BstUI CGCG 4 cut(s) 51, 98, 117, 218
BstV1I GCAGC 1 cut(s) 272
BstZI CGGCCG 1 cut(s) 52
BsuRI GGCC 3 cut(s) 35, 54, 160
BtgI CCRYGG 2 cut(s) 49, 132
Cac8I GCNNGC 7 cut(s) 49, 69, 85, 100, 403, 462, 480
CfoI GCGC 4 cut(s) 98, 104, 319, 371
Cfr13I GGNCC 5 cut(s) 16, 33, 181, 336, 396
Cfr42I CCGCGG 1 cut(s) 52
CpoI CGGWCCG 1 cut(s) 396
CseI GACGC 4 cut(s) 106, 419, 443, 453
Csp6I GTAC 1 cut(s) 221
CspI CGGWCCG 1 cut(s) 396
CviAII CATG 1 cut(s) 110
CviQI GTAC 1 cut(s) 221
DdeI CTNAG 2 cut(s) 455, 489
DinI GGCGCC 2 cut(s) 318, 370
DpnI GATC 2 cut(s) 114, 198
DpnII GATC 2 cut(s) 112, 196
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
EaeI YGGCCR 2 cut(s) 52, 158
EagI CGGCCG 1 cut(s) 52
EciI GGCGGA 1 cut(s) 388
EclXI CGGCCG 1 cut(s) 52
Eco130I CCWWGG 2 cut(s) 12, 256
Eco24I GRGCYC 1 cut(s) 355
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 4 cut(s) 16, 181, 336, 396
Eco52I CGGCCG 1 cut(s) 52
Eco88I CYCGRG 1 cut(s) 189
EcoT14I CCWWGG 2 cut(s) 12, 256
EcoT38I GRGCYC 1 cut(s) 355
EgeI GGCGCC 2 cut(s) 318, 370
EheI GGCGCC 2 cut(s) 318, 370
ErhI CCWWGG 2 cut(s) 12, 256
Esp3I CGTCTC 2 cut(s) 84, 133
FaeI CATG 1 cut(s) 113
FaiI YATR 3 cut(s) 111, 140, 366
FaqI GGGAC 1 cut(s) 397
FatI CATG 1 cut(s) 109
FauI CCCGC 2 cut(s) 56, 453
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 5 cut(s) 52, 161, 164, 167, 286
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 5 cut(s) 52, 161, 164, 167, 286
GlaI GCGC 4 cut(s) 97, 103, 318, 370
GluI GCNGC 5 cut(s) 52, 161, 164, 167, 286
HaeII RGCGCY 2 cut(s) 320, 372
HaeIII GGCC 3 cut(s) 35, 54, 160
HapII CCGG 8 cut(s) 61, 150, 339, 379, 394, 419, 448, 471
HgaI GACGC 4 cut(s) 106, 419, 443, 453
HhaI GCGC 4 cut(s) 98, 104, 319, 371
Hin1I GRCGYC 5 cut(s) 126, 317, 369, 435, 445
Hin1II CATG 1 cut(s) 113
Hin6I GCGC 4 cut(s) 96, 102, 317, 369
HinP1I GCGC 4 cut(s) 96, 102, 317, 369
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 5 cut(s) 8, 192, 305, 451, 495
HpaII CCGG 8 cut(s) 61, 150, 339, 379, 394, 419, 448, 471
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 2 cut(s) 121, 184
Hpy188I TCNGA 2 cut(s) 7, 433
Hpy188III TCNNGA 2 cut(s) 27, 419
Hpy8I GTNNAC 2 cut(s) 121, 184
Hpy99I CGWCG 6 cut(s) 122, 125, 128, 316, 334, 447
HpyAV CCTTC 1 cut(s) 385
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 1 cut(s) 288
HpyF10VI GCNNNNNNNGC 4 cut(s) 93, 160, 166, 316
HpyF3I CTNAG 2 cut(s) 455, 489
HpySE526I ACGT 1 cut(s) 126
Hsp92I GRCGYC 5 cut(s) 126, 317, 369, 435, 445
Hsp92II CATG 1 cut(s) 113
HspAI GCGC 4 cut(s) 96, 102, 317, 369
KasI GGCGCC 2 cut(s) 316, 368
Kpn2I TCCGGA 1 cut(s) 418
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 2 cut(s) 112, 196
LmnI GCTCC 1 cut(s) 64
Lsp1109I GCAGC 1 cut(s) 272
LweI GCATC 1 cut(s) 64
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 3 cut(s) 56, 175, 484
MalI GATC 2 cut(s) 114, 198
MboI GATC 2 cut(s) 112, 196
MhlI GDGCHC 3 cut(s) 69, 345, 355
MluCI AATT 2 cut(s) 265, 427
Mly113I GGCGCC 2 cut(s) 317, 369
MlyI GAGTC 3 cut(s) 2, 201, 299
MnlI CCTC 5 cut(s) 224, 330, 371, 400, 526
MroI TCCGGA 1 cut(s) 418
MslI CAYNNNNRTG 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 8 cut(s) 61, 150, 339, 379, 394, 419, 448, 471
MspR9I CCNGG 6 cut(s) 62, 151, 340, 380, 394, 471
Mva1269I GAATGC 1 cut(s) 288
MvnI CGCG 4 cut(s) 51, 98, 117, 218
MwoI GCNNNNNNNGC 4 cut(s) 93, 160, 166, 316
NarI GGCGCC 2 cut(s) 317, 369
NciI CCSGG 6 cut(s) 62, 151, 340, 380, 394, 471
NdeII GATC 2 cut(s) 112, 196
NlaIII CATG 1 cut(s) 113
NlaIV GGNNCC 3 cut(s) 318, 337, 370
NmeAIII GCCGAG 1 cut(s) 113
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 192
PctI GAATGC 1 cut(s) 288
PfeI GAWTC 2 cut(s) 451, 495
PflFI GACNNNGTC 2 cut(s) 125, 334
PkrI GCNGC 5 cut(s) 53, 162, 165, 168, 287
PleI GAGTC 3 cut(s) 2, 200, 299
PluTI GGCGCC 2 cut(s) 320, 372
PpsI GAGTC 3 cut(s) 2, 200, 299
PspN4I GGNNCC 3 cut(s) 318, 337, 370
PspPI GGNCC 5 cut(s) 16, 33, 181, 336, 396
PsyI GACNNNGTC 2 cut(s) 125, 334
RsaI GTAC 1 cut(s) 222
RsaNI GTAC 1 cut(s) 221
RseI CAYNNNNRTG 1 cut(s) 72
Rsr2I CGGWCCG 1 cut(s) 396
RsrII CGGWCCG 1 cut(s) 396
SacII CCGCGG 1 cut(s) 52
SalI GTCGAC 1 cut(s) 119
SatI GCNGC 5 cut(s) 52, 161, 164, 167, 286
Sau3AI GATC 2 cut(s) 112, 196
Sau96I GGNCC 5 cut(s) 16, 33, 181, 336, 396
SchI GAGTC 3 cut(s) 2, 201, 299
ScrFI CCNGG 6 cut(s) 62, 151, 340, 380, 394, 471
SduI GDGCHC 3 cut(s) 69, 345, 355
SetI ASST 1 cut(s) 129
SfaNI GCATC 1 cut(s) 64
SfoI GGCGCC 2 cut(s) 318, 370
Sfr303I CCGCGG 1 cut(s) 52
SgrBI CCGCGG 1 cut(s) 52
SgrDI CGTCGACG 1 cut(s) 119
SinI GGWCC 4 cut(s) 16, 181, 336, 396
SmiMI CAYNNNNRTG 1 cut(s) 72
Sse9I AATT 2 cut(s) 265, 427
SspDI GGCGCC 2 cut(s) 316, 368
StyD4I CCNGG 6 cut(s) 60, 149, 338, 378, 392, 469
StyI CCWWGG 2 cut(s) 12, 256
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 1 cut(s) 129
TaqI TCGA 2 cut(s) 120, 195
TaqII GACCGA 1 cut(s) 225
TasI AATT 2 cut(s) 265, 427
TauI GCSGC 4 cut(s) 54, 163, 166, 169
TfiI GAWTC 2 cut(s) 451, 495
TseI GCWGC 1 cut(s) 285
TspDTI ATGAA 1 cut(s) 194
TspGWI ACGGA 3 cut(s) 8, 163, 277
Tth111I GACNNNGTC 2 cut(s) 125, 334
VpaK11BI GGWCC 4 cut(s) 16, 181, 336, 396
XapI RAATTY 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 120
ZraI GACGTC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.