pycom395g00050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000395
OG0011893
Physical Location & Seq
Forward (+)
113446 .. 115293
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1137 bp
ATGAGTAGTGATAATTCAACCAATGAGTCTTGCAAATCCCGACGGGTTCTCGGTGATTTGACCAACCGGCCCATAAAAAGGGGGTTTTTGATGATTTCAGCTGGTTCGGGTGCTAAATCTCGAGATGAGTTCGGTAAAGATGTTGATTTGGAAAATAGGGGTTCGAAATTTGCAAAGCAATTGTCTTTAGGGGTTGAGGATTTAGTAGGAGAGAAATGCAAGACCAAAACTGGAGCAGATTGTAACCCTGAAGCTTTGTCTTCGCCAAAGGGCACACAGGAATCAGTGTTTTCGCCTACTTATAGTGACATTGATGATTCGTCGAATGACGACAGCGATTCTGTTACCTTGACTATGTCAAATGATATGGGGGAAAAGTCTAGTGACAGTGGAGCTAATGCGCGCAGTGCTCTAGAAGCCGGTGATGCATTGATGGATAATTGCTCGTCCATTGCTTCAATTTCAAAATGTTCAGGAATGTGTAAGAATGACTGTTGTGGTGATGGGGGGAAATGTCAATATGATAAACCCAGACACATTTCAGAAGTTAATCAAAGTGTTCCTCTATATGAAGGATTAGTCGCTGTTGTTTGCGGTAATCACGAAAAGGATCTTGGAGTTGGTAAAGTGGCAACAACCAAATATGGTTCTAATGAGTGGTCGAGGTTGCCTAGGTCGCAAAGTTGCTCGAAATCCCACGAATTAAAAAAGTTGGAAAGATGCACGACTCTTAAAGGTGGTGGCAGTGCTGATTTGAATGTAGGTGAATACTTGCTCAAAGATTGCTCTTGTTCGTTTTGCTTGAAAGCTGCTCACATCTTGTCGGATCTCCAATACCAGGATATCAAGGGTCGAATTGCTGCATTGAAGAGGAGTAAAAAAGAAGCGAGCATATTGGTTGAGAAATGTGTCAGGGGAAAGGAGATTAATACTAAGGACCGACTACATCCAAACAAAGCGTCAAAAAGTGAATATGATCTCTCGAGTCAGTGGAGATCACTCTTTCTTAACATGGAGGATGTGCTTGTTCATGAAAGCAACCATCTTGAAGACAGTTATGTCACACTAAAAAATTTGAGGGACAACTGCAAGACGGAACTTGAAATTACCAGTGGAATGCCTTCCGAGAAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

41.15

Weight (kDa)

6.22

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10110 AT5G65120 AT5G65120
fragaria_vesca FvH4_1g21240
malus_domestica MD01G1027000.v1.1 MD15G1327000.v1.1
prunus_persica Prupe.6G182000_v2.0.a1 Prupe.6G182000_v2.0.a1
pyrus_communis pycom01g05490 pycom395g00050
rosa_chinensis RchiOBHm_Chr2g0115081
rosa_laevigata RLG00000018155
rosa_multiflora Rmu_sc0002784.1_g000001
rosa_roxburghii Rroxscaffold_2G00130550
rosa_rugosa Rorug02G0193200
rosa_samantha Rh2AG251700 Rh2BG261200 Rh2DG259800
rosa_wichuraiana Rw2G019630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1058
AccII CGCG 1 cut(s) 403
AciI CCGC 1 cut(s) 594
AclWI GGATC 2 cut(s) 618, 834
AcsI RAATTY 2 cut(s) 167, 1072
AcuI CTGAAG 1 cut(s) 270
AfiI CCNNNNNNNGG 2 cut(s) 78, 838
AgsI TTSAA 8 cut(s) 18, 459, 465, 757, 805, 868, 1049, 1103
AjnI CCWGG 1 cut(s) 837
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
AluBI AGCT 4 cut(s) 101, 254, 395, 809
AluI AGCT 4 cut(s) 101, 254, 395, 809
Alw21I GWGCWC 1 cut(s) 412
AlwI GGATC 2 cut(s) 618, 834
Ama87I CYCGRG 2 cut(s) 120, 982
AoxI GGCC 1 cut(s) 68
ApeKI GCWGC 2 cut(s) 809, 860
ApoI RAATTY 2 cut(s) 167, 1072
ArsI GACNNNNNNTTYG 4 cut(s) 167, 199, 944, 976
AseI ATTAAT 1 cut(s) 927
Asp700I GAANNNNTTC 1 cut(s) 1120
AspA2I CCTAGG 1 cut(s) 671
AspLEI GCGC 2 cut(s) 403, 405
AspS9I GGNCC 2 cut(s) 69, 937
AsuHPI GGTGA 4 cut(s) 65, 434, 512, 776
AsuII TTCGAA 1 cut(s) 164
AvaI CYCGRG 2 cut(s) 120, 982
AvaII GGWCC 1 cut(s) 937
AvrII CCTAGG 1 cut(s) 671
BaeGI GKGCMC 1 cut(s) 275
BbsI GAAGAC 2 cut(s) 252, 1056
Bbv12I GWGCWC 1 cut(s) 412
BbvI GCAGC 2 cut(s) 796, 847
BccI CCATC 3 cut(s) 427, 497, 1050
BciT130I CCWGG 1 cut(s) 839
BfaI CTAG 3 cut(s) 381, 413, 672
BisI GCNGC 2 cut(s) 810, 861
BlnI CCTAGG 1 cut(s) 671
BlsI GCNGC 2 cut(s) 811, 862
Bme1390I CCNGG 1 cut(s) 839
Bme18I GGWCC 1 cut(s) 937
BmeT110I CYCGRG 2 cut(s) 120, 982
BmgT120I GGNCC 2 cut(s) 69, 937
BmrFI CCNGG 1 cut(s) 839
BmsI GCATC 2 cut(s) 415, 710
BpiI GAAGAC 2 cut(s) 252, 1056
BpmI CTGGAG 1 cut(s) 252
Bpu14I TTCGAA 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 671
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 838
Bse118I RCCGGY 2 cut(s) 66, 419
Bse1I ACTGG 2 cut(s) 235, 1110
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 1 cut(s) 839
BseDI CCNNGG 1 cut(s) 671
BseGI GGATG 2 cut(s) 946, 1024
BseLI CCNNNNNNNGG 2 cut(s) 78, 838
BseMI GCAATG 1 cut(s) 450
BseNI ACTGG 2 cut(s) 235, 1110
BsePI GCGCGC 1 cut(s) 401
BseRI GAGGAG 1 cut(s) 886
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 2 cut(s) 796, 847
Bsh1236I CGCG 1 cut(s) 403
BshFI GGCC 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 412
BsiHKCI CYCGRG 2 cut(s) 120, 982
BsiSI CCGG 2 cut(s) 67, 420
BslFI GGGAC 1 cut(s) 1094
BslI CCNNNNNNNGG 2 cut(s) 78, 838
BsmFI GGGAC 1 cut(s) 1094
BsmI GAATGC 1 cut(s) 1122
BsnI GGCC 1 cut(s) 70
BsoBI CYCGRG 2 cut(s) 120, 982
Bsp119I TTCGAA 1 cut(s) 164
Bsp1286I GDGCHC 2 cut(s) 275, 412
Bsp143I GATC 4 cut(s) 610, 826, 976, 995
BspACI CCGC 1 cut(s) 594
BspANI GGCC 1 cut(s) 70
BspFNI CGCG 1 cut(s) 403
BspHI TCATGA 1 cut(s) 1030
BspPI GGATC 2 cut(s) 618, 834
BspT104I TTCGAA 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 2 cut(s) 66, 419
BsrI ACTGG 2 cut(s) 235, 1110
BssAI RCCGGY 2 cut(s) 66, 419
BssECI CCNNGG 1 cut(s) 671
BssHII GCGCGC 1 cut(s) 401
BssMI GATC 4 cut(s) 610, 826, 976, 995
BssT1I CCWWGG 1 cut(s) 671
Bst2UI CCWGG 1 cut(s) 839
Bst4CI ACNGT 3 cut(s) 389, 494, 1055
Bst6I CTCTTC 1 cut(s) 863
BstBI TTCGAA 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 403, 889
BstDEI CTNAG 1 cut(s) 933
BstF5I GGATG 2 cut(s) 946, 1024
BstFNI CGCG 1 cut(s) 403
BstHHI GCGC 2 cut(s) 403, 405
BstKTI GATC 4 cut(s) 613, 829, 979, 998
BstMBI GATC 4 cut(s) 610, 826, 976, 995
BstMWI GCNNNNNNNGC 4 cut(s) 407, 416, 425, 676
BstNI CCWGG 1 cut(s) 839
BstSCI CCNGG 1 cut(s) 837
BstSLI GKGCMC 1 cut(s) 275
BstUI CGCG 1 cut(s) 403
BstV1I GCAGC 2 cut(s) 796, 847
BstV2I GAAGAC 2 cut(s) 252, 1056
BstX2I RGATCY 2 cut(s) 610, 826
BstYI RGATCY 2 cut(s) 610, 826
BsuRI GGCC 1 cut(s) 70
BtsCI GGATG 2 cut(s) 946, 1024
BtsI GCAGTG 2 cut(s) 412, 751
BtsIMutI CAGTG 6 cut(s) 291, 394, 412, 751, 995, 1117
Cac8I GCNNGC 2 cut(s) 403, 889
CciI TCATGA 1 cut(s) 1030
CfoI GCGC 2 cut(s) 403, 405
Cfr10I RCCGGY 2 cut(s) 66, 419
Cfr13I GGNCC 2 cut(s) 69, 937
CseI GACGC 1 cut(s) 948
CviAII CATG 2 cut(s) 1012, 1031
CviJI RGCY 6 cut(s) 70, 101, 254, 395, 419, 809
CviKI_1 RGCY 6 cut(s) 70, 101, 254, 395, 419, 809
DdeI CTNAG 1 cut(s) 933
DpnI GATC 4 cut(s) 612, 828, 978, 997
DpnII GATC 4 cut(s) 610, 826, 976, 995
DrdI GACNNNNNNGTC 1 cut(s) 1058
DseDI GACNNNNNNGTC 1 cut(s) 1058
Eam1104I CTCTTC 1 cut(s) 863
EarI CTCTTC 1 cut(s) 863
Eco130I CCWWGG 1 cut(s) 671
Eco32I GATATC 1 cut(s) 844
Eco47I GGWCC 1 cut(s) 937
Eco57I CTGAAG 1 cut(s) 270
Eco88I CYCGRG 2 cut(s) 120, 982
EcoRII CCWGG 1 cut(s) 837
EcoRV GATATC 1 cut(s) 844
EcoT14I CCWWGG 1 cut(s) 671
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 671
FaeI CATG 2 cut(s) 1015, 1034
FaqI GGGAC 1 cut(s) 1094
FatI CATG 2 cut(s) 1011, 1030
Fnu4HI GCNGC 2 cut(s) 810, 861
FokI GGATG 2 cut(s) 933, 1031
Fsp4HI GCNGC 2 cut(s) 810, 861
FspBI CTAG 3 cut(s) 381, 413, 672
GlaI GCGC 2 cut(s) 402, 404
GluI GCNGC 2 cut(s) 810, 861
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 1 cut(s) 70
HapII CCGG 2 cut(s) 67, 420
HgaI GACGC 1 cut(s) 948
HhaI GCGC 2 cut(s) 403, 405
Hin1II CATG 2 cut(s) 1015, 1034
Hin6I GCGC 2 cut(s) 401, 403
HinP1I GCGC 2 cut(s) 401, 403
HindIII AAGCTT 1 cut(s) 252
HinfI GANTC 6 cut(s) 26, 281, 317, 338, 727, 985
HpaII CCGG 2 cut(s) 67, 420
HphI GGTGA 4 cut(s) 65, 434, 512, 776
Hpy188I TCNGA 3 cut(s) 544, 826, 1126
Hpy188III TCNNGA 9 cut(s) 39, 120, 122, 413, 474, 602, 982, 1031, 1046
Hpy99I CGWCG 2 cut(s) 45, 325
HpyAV CCTTC 2 cut(s) 566, 1131
HpyCH4III ACNGT 3 cut(s) 389, 494, 1055
HpyCH4V TGCA 7 cut(s) 33, 173, 219, 428, 723, 863, 1089
HpyF10VI GCNNNNNNNGC 4 cut(s) 407, 416, 425, 676
HpyF3I CTNAG 1 cut(s) 933
Hsp92II CATG 2 cut(s) 1015, 1034
HspAI GCGC 2 cut(s) 401, 403
Kzo9I GATC 4 cut(s) 610, 826, 976, 995
LmnI GCTCC 2 cut(s) 233, 392
Lsp1109I GCAGC 2 cut(s) 796, 847
LweI GCATC 2 cut(s) 415, 710
MaeI CTAG 3 cut(s) 381, 413, 672
MaeIII GTNAC 5 cut(s) 242, 305, 343, 383, 1060
MalI GATC 4 cut(s) 612, 828, 978, 997
MboI GATC 4 cut(s) 610, 826, 976, 995
MboII GAAGA 3 cut(s) 252, 880, 1061
MfeI CAATTG 1 cut(s) 179
MflI RGATCY 2 cut(s) 610, 826
MhlI GDGCHC 2 cut(s) 275, 412
MluCI AATT 9 cut(s) 13, 167, 179, 439, 459, 701, 855, 1072, 1104
MlyI GAGTC 3 cut(s) 35, 721, 994
MmeI TCCRAC 2 cut(s) 693, 804
MnlI CCTC 6 cut(s) 190, 573, 657, 864, 1009, 1071
Mph1103I ATGCAT 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 1120
MseI TTAA 5 cut(s) 549, 704, 732, 927, 1008
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 2 cut(s) 67, 420
MspR9I CCNGG 1 cut(s) 839
MunI CAATTG 1 cut(s) 179
Mva1269I GAATGC 1 cut(s) 1122
MvaI CCWGG 1 cut(s) 839
MvnI CGCG 1 cut(s) 403
MwoI GCNNNNNNNGC 4 cut(s) 407, 416, 425, 676
NdeII GATC 4 cut(s) 610, 826, 976, 995
NlaIII CATG 2 cut(s) 1015, 1034
NmuCI GTSAC 3 cut(s) 305, 383, 1060
NsiI ATGCAT 1 cut(s) 430
NspV TTCGAA 1 cut(s) 164
PaeR7I CTCGAG 2 cut(s) 120, 982
PagI TCATGA 1 cut(s) 1030
PauI GCGCGC 1 cut(s) 401
PctI GAATGC 1 cut(s) 1122
PdmI GAANNNNTTC 1 cut(s) 1120
PfeI GAWTC 3 cut(s) 281, 317, 338
PflFI GACNNNGTC 1 cut(s) 355
PkrI GCNGC 2 cut(s) 811, 862
PleI GAGTC 3 cut(s) 34, 721, 993
PpsI GAGTC 3 cut(s) 34, 721, 993
PshBI ATTAAT 1 cut(s) 927
Psp6I CCWGG 1 cut(s) 837
PspGI CCWGG 1 cut(s) 837
PspPI GGNCC 2 cut(s) 69, 937
PsuI RGATCY 2 cut(s) 610, 826
PsyI GACNNNGTC 1 cut(s) 355
PteI GCGCGC 1 cut(s) 401
PvuII CAGCTG 1 cut(s) 101
SaqAI TTAA 5 cut(s) 549, 704, 732, 927, 1008
SatI GCNGC 2 cut(s) 810, 861
Sau3AI GATC 4 cut(s) 610, 826, 976, 995
Sau96I GGNCC 2 cut(s) 69, 937
SchI GAGTC 3 cut(s) 35, 721, 994
ScrFI CCNGG 1 cut(s) 839
SduI GDGCHC 2 cut(s) 275, 412
SetI ASST 9 cut(s) 103, 256, 350, 397, 668, 677, 739, 766, 811
SfaNI GCATC 2 cut(s) 415, 710
Sfr274I CTCGAG 2 cut(s) 120, 982
SfuI TTCGAA 1 cut(s) 164
SinI GGWCC 1 cut(s) 937
SlaI CTCGAG 2 cut(s) 120, 982
SmlI CTYRAG 2 cut(s) 120, 982
SmoI CTYRAG 2 cut(s) 120, 982
Sse9I AATT 9 cut(s) 13, 167, 179, 439, 459, 701, 855, 1072, 1104
SsiI CCGC 1 cut(s) 594
SspMI CTAG 3 cut(s) 381, 413, 672
StyD4I CCNGG 1 cut(s) 837
StyI CCWWGG 1 cut(s) 671
TaaI ACNGT 3 cut(s) 389, 494, 1055
TaqI TCGA 7 cut(s) 121, 164, 323, 662, 689, 853, 983
TaqII GACCGA 1 cut(s) 954
TasI AATT 9 cut(s) 13, 167, 179, 439, 459, 701, 855, 1072, 1104
TfiI GAWTC 3 cut(s) 281, 317, 338
Tru1I TTAA 5 cut(s) 549, 704, 732, 927, 1008
Tru9I TTAA 5 cut(s) 549, 704, 732, 927, 1008
TscAI CASTG 6 cut(s) 291, 394, 412, 751, 995, 1117
TseFI GTSAC 3 cut(s) 305, 383, 1060
TseI GCWGC 2 cut(s) 809, 860
Tsp45I GTSAC 3 cut(s) 305, 383, 1060
TspDTI ATGAA 3 cut(s) 585, 1019, 1047
TspGWI ACGGA 1 cut(s) 1109
TspRI CASTG 6 cut(s) 291, 394, 412, 751, 995, 1117
Tth111I GACNNNGTC 1 cut(s) 355
VpaK11BI GGWCC 1 cut(s) 937
VspI ATTAAT 1 cut(s) 927
XapI RAATTY 2 cut(s) 167, 1072
XbaI TCTAGA 1 cut(s) 412
XhoI CTCGAG 2 cut(s) 120, 982
XmaJI CCTAGG 1 cut(s) 671
XmnI GAANNNNTTC 1 cut(s) 1120
XspI CTAG 3 cut(s) 381, 413, 672
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.