pycom420g00660

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0005480
Physical Location & Seq
Reverse (-)
808438 .. 809660
1223 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1128 bp
ATGACATTGGCTGGTTTTGATGGTTTGCGAATTCAGTGTTCTGATCAGAGTGTTCTTTTCCTCAGTGATGGGTCTGACGATGGTATGGTGCCTCCCGGGTCACTTTGCATGATCTCGCACAAGGATAAGGAGGTGATGAATGCTTTGGATGGTGCTGGTTCTCAAGCAACTGAAGAAGAGGTTCGGCAAGAGGTGGTTGCAATGTCTCACACTAACATATTCAGCCCTGGGATTGATGTGACTCAGGCAGTACTTTTACCTCAGATGACCTGGAGGTGGCAGGAGAAAACAGAAATGGCTGCCCTATCAAATCTAGAAGAGTCCCCTGGGGCTATGACTGATGACGAGCTCTTCTCATCCTGCAATGAAAATGAAACAGAACGTGAGCAGTTTAATGATATTCTGACAGAGGATGAGACGAGGCAACCTGAAAATGCACTTAAATTGGATTCGTCAGAATTGTCAAACGAAAATGGTGATGAGATTATTGAGCATCGTCATAGTTGTTATGATCACAGGGAGATTCGTATTGAAGATGTTAGCAGTGGTAGAAATGGAGAGAATAAGCAGGAAAAGAAAGGATGGTTTGGTGGATGGAGGAAATGGGGTACTAAAAATGAAGGGCAGAAAAAGATTGTGCCGCCTGAAGTTCTCTGTGTAGATGAGAAGAGTAATGTGGACATTATTGTGAAGGGTGATGATCACCGGAGAGGAAGGGATATGAAAGCATCTTCTTCCACAAGTTCTGATAAGAGGAATCGTCACAAGGGTGCTAGCAAGGAGAATGAGTATAAGAAGGGATCGAGGCCAATACTCTGGCTTTCCCCAAACTTTCCATTACAAACAGAGGAACTGTTGCTGTTACTTGACATTTTAGCAAACAAGGTTAAGGCAATTCGTCGTTTGAGGGAGCTGCTTACAACCAAACTTCCAATGGGTACATTTCCAGTCAAGGTATGCCACCTTCCCATTATCATTTTGTTACCCAGTATGGTTTATGGTTTCCTAGATGCACCTTTGTTATCTTTTCCTTTTTATCAAACTTGTTCAGAAAACATTTTGTTATTTTCTTTTTTAACCATGCCTGGTTCTGTTGTAACTGTAAGAGTAGGATGTGCTCTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_5 GPCR_chapero_1

Protein Analysis

376

Amino Acids

42.1

Weight (kDa)

5.12

Isoelectric Point (pI)

60.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANKRD13_C PF11904 1 - 325 7.7e-67 ANKRD13 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 88
AciI CCGC 1 cut(s) 641
AclWI GGATC 1 cut(s) 808
AcsI RAATTY 1 cut(s) 30
AcuI CTGAAG 2 cut(s) 192, 666
AfaI GTAC 3 cut(s) 252, 610, 940
AfiI CCNNNNNNNGG 1 cut(s) 276
AgsI TTSAA 1 cut(s) 533
AjnI CCWGG 4 cut(s) 226, 269, 325, 1084
AleI CACNNNNGTG 1 cut(s) 768
AluBI AGCT 2 cut(s) 349, 913
AluI AGCT 2 cut(s) 349, 913
Alw21I GWGCWC 2 cut(s) 351, 1120
Alw26I GTCTC 2 cut(s) 210, 410
AlwI GGATC 1 cut(s) 808
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 1 cut(s) 806
ApeKI GCWGC 2 cut(s) 299, 913
ApoI RAATTY 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 180
AsuC2I CCSGG 2 cut(s) 96, 97
AsuHPI GGTGA 4 cut(s) 145, 488, 695, 707
AsuNHI GCTAGC 1 cut(s) 773
AvaI CYCGRG 1 cut(s) 95
BanI GGYRCC 1 cut(s) 88
BanII GRGCYC 1 cut(s) 351
BarI GAAGNNNNNNTAC 2 cut(s) 948, 980
Bbv12I GWGCWC 2 cut(s) 351, 1120
BbvI GCAGC 2 cut(s) 286, 900
BccI CCATC 6 cut(s) 14, 62, 74, 143, 576, 588
BciT130I CCWGG 4 cut(s) 228, 271, 327, 1086
BclI TGATCA 3 cut(s) 43, 511, 700
BcnI CCSGG 2 cut(s) 96, 97
BcoDI GTCTC 2 cut(s) 210, 410
BfaI CTAG 3 cut(s) 314, 774, 1007
BisI GCNGC 3 cut(s) 300, 641, 914
BlsI GCNGC 3 cut(s) 301, 642, 915
BmcAI AGTACT 1 cut(s) 252
Bme1390I CCNGG 6 cut(s) 96, 97, 228, 271, 327, 1086
BmeT110I CYCGRG 1 cut(s) 95
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 6 cut(s) 96, 97, 228, 271, 327, 1086
BmrI ACTGGG 1 cut(s) 981
BmsI GCATC 3 cut(s) 502, 737, 1000
BmtI GCTAGC 1 cut(s) 777
BmuI ACTGGG 1 cut(s) 981
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpmI CTGGAG 1 cut(s) 292
BpuEI CTTGAG 1 cut(s) 147
BpuMI CCSGG 2 cut(s) 96, 97
BsaJI CCNNGG 5 cut(s) 95, 226, 227, 325, 326
BsaWI WCCGGW 1 cut(s) 705
BsaXI ACNNNNNCTCC 2 cut(s) 773, 803
Bsc4I CCNNNNNNNGG 1 cut(s) 276
Bse1I ACTGG 2 cut(s) 947, 987
Bse3DI GCAATG 2 cut(s) 207, 370
BseBI CCWGG 4 cut(s) 228, 271, 327, 1086
BseDI CCNNGG 5 cut(s) 95, 226, 227, 325, 326
BseGI GGATG 6 cut(s) 154, 356, 418, 587, 599, 1118
BseLI CCNNNNNNNGG 1 cut(s) 276
BseMI GCAATG 2 cut(s) 207, 370
BseMII CTCAG 3 cut(s) 76, 257, 275
BseNI ACTGG 2 cut(s) 947, 987
BseXI GCAGC 2 cut(s) 286, 900
BshFI GGCC 1 cut(s) 808
BshNI GGYRCC 1 cut(s) 88
BsiHKAI GWGCWC 2 cut(s) 351, 1120
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 2 cut(s) 96, 706
BslFI GGGAC 1 cut(s) 307
BslI CCNNNNNNNGG 1 cut(s) 276
BsmAI GTCTC 2 cut(s) 210, 410
BsmBI CGTCTC 1 cut(s) 410
BsmFI GGGAC 1 cut(s) 307
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 1 cut(s) 808
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 351, 1120
Bsp143I GATC 5 cut(s) 43, 111, 511, 700, 800
BspACI CCGC 1 cut(s) 641
BspANI GGCC 1 cut(s) 808
BspCNI CTCAG 3 cut(s) 75, 256, 274
BspLI GGNNCC 1 cut(s) 90
BspOI GCTAGC 1 cut(s) 777
BspPI GGATC 1 cut(s) 808
BspQI GCTCTTC 1 cut(s) 356
BspT107I GGYRCC 1 cut(s) 88
BsrDI GCAATG 2 cut(s) 207, 370
BsrI ACTGG 2 cut(s) 947, 987
BssECI CCNNGG 5 cut(s) 95, 226, 227, 325, 326
BssMI GATC 5 cut(s) 43, 111, 511, 700, 800
Bst2UI CCWGG 4 cut(s) 228, 271, 327, 1086
Bst4CI ACNGT 2 cut(s) 855, 1102
Bst6I CTCTTC 4 cut(s) 171, 312, 356, 662
BstC8I GCNNGC 1 cut(s) 775
BstDEI CTNAG 3 cut(s) 62, 243, 261
BstF5I GGATG 6 cut(s) 154, 356, 418, 587, 599, 1118
BstKTI GATC 5 cut(s) 46, 114, 514, 703, 803
BstMAI GTCTC 2 cut(s) 210, 410
BstMBI GATC 5 cut(s) 43, 111, 511, 700, 800
BstNI CCWGG 4 cut(s) 228, 271, 327, 1086
BstSCI CCNGG 6 cut(s) 94, 95, 226, 269, 325, 1084
BstV1I GCAGC 2 cut(s) 286, 900
BstXI CCANNNNNNTGG 1 cut(s) 816
BsuRI GGCC 1 cut(s) 808
BtsCI GGATG 6 cut(s) 154, 356, 418, 587, 599, 1118
BtsI GCAGTG 1 cut(s) 550
BtsIMutI CAGTG 3 cut(s) 41, 70, 550
Cac8I GCNNGC 1 cut(s) 775
Cfr9I CCCGGG 1 cut(s) 95
Csp6I GTAC 3 cut(s) 251, 609, 939
CviAII CATG 2 cut(s) 109, 1081
CviJI RGCY 8 cut(s) 11, 225, 299, 332, 349, 808, 820, 913
CviKI_1 RGCY 8 cut(s) 11, 225, 299, 332, 349, 808, 820, 913
CviQI GTAC 3 cut(s) 251, 609, 939
DdeI CTNAG 3 cut(s) 62, 243, 261
DpnI GATC 5 cut(s) 45, 113, 513, 702, 802
DpnII GATC 5 cut(s) 43, 111, 511, 700, 800
Eam1104I CTCTTC 4 cut(s) 171, 312, 356, 662
EarI CTCTTC 4 cut(s) 171, 312, 356, 662
Ecl136II GAGCTC 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 349
Eco57I CTGAAG 2 cut(s) 192, 666
Eco88I CYCGRG 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 349
EcoRI GAATTC 1 cut(s) 30
EcoRII CCWGG 4 cut(s) 226, 269, 325, 1084
EcoT38I GRGCYC 1 cut(s) 351
Esp3I CGTCTC 1 cut(s) 410
FaeI CATG 2 cut(s) 112, 1084
FaqI GGGAC 1 cut(s) 307
FatI CATG 2 cut(s) 108, 1080
FbaI TGATCA 3 cut(s) 43, 511, 700
Fnu4HI GCNGC 3 cut(s) 300, 641, 914
FokI GGATG 5 cut(s) 161, 343, 425, 594, 606
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 3 cut(s) 300, 641, 914
FspBI CTAG 3 cut(s) 314, 774, 1007
GluI GCNGC 3 cut(s) 300, 641, 914
GsuI CTGGAG 1 cut(s) 292
HaeIII GGCC 1 cut(s) 808
HapII CCGG 2 cut(s) 96, 706
Hin1II CATG 2 cut(s) 112, 1084
HinfI GANTC 5 cut(s) 241, 320, 449, 523, 757
HpaII CCGG 2 cut(s) 96, 706
HphI GGTGA 4 cut(s) 145, 488, 695, 707
Hpy166II GTNNAC 1 cut(s) 679
Hpy188I TCNGA 8 cut(s) 43, 48, 76, 264, 405, 457, 748, 1051
Hpy188III TCNNGA 1 cut(s) 314
Hpy8I GTNNAC 1 cut(s) 679
Hpy99I CGWCG 1 cut(s) 903
HpyAV CCTTC 5 cut(s) 614, 685, 708, 790, 974
HpyCH4III ACNGT 2 cut(s) 855, 1102
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 5 cut(s) 108, 200, 363, 437, 1013
HpyF3I CTNAG 3 cut(s) 62, 243, 261
HpySE526I ACGT 1 cut(s) 382
Hsp92II CATG 2 cut(s) 112, 1084
Ksp22I TGATCA 3 cut(s) 43, 511, 700
Kzo9I GATC 5 cut(s) 43, 111, 511, 700, 800
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 1 cut(s) 910
Lsp1109I GCAGC 2 cut(s) 286, 900
LweI GCATC 3 cut(s) 502, 737, 1000
MaeI CTAG 3 cut(s) 314, 774, 1007
MaeII ACGT 1 cut(s) 382
MaeIII GTNAC 6 cut(s) 99, 238, 761, 861, 981, 1096
MalI GATC 5 cut(s) 45, 113, 513, 702, 802
MboI GATC 5 cut(s) 43, 111, 511, 700, 800
MboII GAAGA 8 cut(s) 185, 188, 329, 343, 545, 679, 723, 726
MhlI GDGCHC 2 cut(s) 351, 1120
MluCI AATT 4 cut(s) 30, 443, 458, 894
MlyI GAGTC 2 cut(s) 235, 329
MroXI GAANNNNTTC 1 cut(s) 180
MseI TTAA 4 cut(s) 393, 441, 888, 1076
MslI CAYNNNNRTG 2 cut(s) 686, 768
MspI CCGG 2 cut(s) 96, 706
MspR9I CCNGG 6 cut(s) 96, 97, 228, 271, 327, 1086
Mva1269I GAATGC 1 cut(s) 145
MvaI CCWGG 4 cut(s) 228, 271, 327, 1086
NciI CCSGG 2 cut(s) 96, 97
NdeII GATC 5 cut(s) 43, 111, 511, 700, 800
NheI GCTAGC 1 cut(s) 773
NlaIII CATG 2 cut(s) 112, 1084
NlaIV GGNNCC 1 cut(s) 90
NmuCI GTSAC 3 cut(s) 99, 238, 761
OliI CACNNNNGTG 1 cut(s) 768
PasI CCCWGGG 2 cut(s) 227, 326
PciSI GCTCTTC 1 cut(s) 356
PctI GAATGC 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 180
PfeI GAWTC 3 cut(s) 449, 523, 757
PkrI GCNGC 3 cut(s) 301, 642, 915
PleI GAGTC 2 cut(s) 235, 328
PpsI GAGTC 2 cut(s) 235, 328
Psp124BI GAGCTC 1 cut(s) 351
Psp6I CCWGG 4 cut(s) 226, 269, 325, 1084
PspGI CCWGG 4 cut(s) 226, 269, 325, 1084
PspN4I GGNNCC 1 cut(s) 90
RsaI GTAC 3 cut(s) 252, 610, 940
RsaNI GTAC 3 cut(s) 251, 609, 939
RseI CAYNNNNRTG 2 cut(s) 686, 768
SacI GAGCTC 1 cut(s) 351
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 4 cut(s) 393, 441, 888, 1076
SatI GCNGC 3 cut(s) 300, 641, 914
Sau3AI GATC 5 cut(s) 43, 111, 511, 700, 800
ScaI AGTACT 1 cut(s) 252
SchI GAGTC 2 cut(s) 235, 329
ScrFI CCNGG 6 cut(s) 96, 97, 228, 271, 327, 1086
SduI GDGCHC 2 cut(s) 351, 1120
SfaNI GCATC 3 cut(s) 502, 737, 1000
SmaI CCCGGG 1 cut(s) 97
SmiMI CAYNNNNRTG 2 cut(s) 686, 768
SmlI CTYRAG 1 cut(s) 162
SmoI CTYRAG 1 cut(s) 162
Sse9I AATT 4 cut(s) 30, 443, 458, 894
SsiI CCGC 1 cut(s) 641
SspMI CTAG 3 cut(s) 314, 774, 1007
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 6 cut(s) 94, 95, 226, 269, 325, 1084
TaaI ACNGT 2 cut(s) 855, 1102
TaiI ACGT 1 cut(s) 385
TaqI TCGA 1 cut(s) 803
TasI AATT 4 cut(s) 30, 443, 458, 894
TatI WGTACW 1 cut(s) 250
TauI GCSGC 1 cut(s) 643
TfiI GAWTC 3 cut(s) 449, 523, 757
Tru1I TTAA 4 cut(s) 393, 441, 888, 1076
Tru9I TTAA 4 cut(s) 393, 441, 888, 1076
TscAI CASTG 3 cut(s) 41, 70, 550
TseFI GTSAC 3 cut(s) 99, 238, 761
TseI GCWGC 2 cut(s) 299, 913
Tsp45I GTSAC 3 cut(s) 99, 238, 761
TspDTI ATGAA 5 cut(s) 152, 381, 387, 633, 737
TspMI CCCGGG 1 cut(s) 95
TspRI CASTG 3 cut(s) 41, 70, 550
XapI RAATTY 1 cut(s) 30
XbaI TCTAGA 1 cut(s) 313
XcmI CCANNNNNNNNNTGG 1 cut(s) 931
XmaI CCCGGG 1 cut(s) 95
XmnI GAANNNNTTC 1 cut(s) 180
XspI CTAG 3 cut(s) 314, 774, 1007
ZrmI AGTACT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.