pycom461g00360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0017129
Physical Location & Seq
Forward (+)
292977 .. 294160
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom461g00360.2

Sequence Viewer

Length: 900 bp
ATGACACGGTTATTCGATGTTGTACTCCGACTTAAGCCACTACAGTGGCAGAAGATTTTAGAAGATCTTCGTAGCATCAAGGGGATTCTTTCTAAGGTCCAATTGATACTATTCGACGGTTATACTAAGGAGTACTGTATTGCCACTCACCTATTCGCTCGAAGAGTACTGAGCTTAGTTAGACGTTCAGGATTTCTGTCCACTGCCTTATATTTGAAGCAGTGTTCATCTTCACTGATGATCGCTTATGGTGGCGATCGTCGTGAACCTGAACTATTGTCTGTTCCAGTATCTCTGACTCGTCGAGGGTACCCTCGTATAATTCCCGCGTTCCATTGTCGCGTGATCTACGGTGGTGGTCCTCGGGCTGATGAATGCGTTCGGTTTTATCTTTCTGTATTTTCTTTGTATCGGATTGTTATCCTTGCAAAGAAGATATCAAAGAATACTTTTGAACCGTTCATCACTCCGATTAAGGATATGGATCGTGTGCTTAGTTCCATCCAAGCTCGGTTTTGTCTGAATCTTCGCGGAAGAAGAAGAAGCGGTTCACCAGCCACTACATCTGTGGATCCCACCAAATCATTAAACCTGGCGGCTGCTCGCTCTTTGATCAGTGATTCACCGGCCACTACATCGATGAAGAATCTCTCCAAAATCTGCTTTTTGATCAGTGATTCACCGGCCACTACATTCAGGGATCCCACCTTATTCTCAAACCTGGCGGCTCGGTTGATTGGCACTCCGGTAGGCTCATCTTGCATACAAATTCTGGGGGTCCCAGGTCCTGCATCCACCAAGAACATTTCTTCCCTTAAGCGTAAAACTTCAGATTGTAAGGCATTTCCACTACATAAGAAAAAACTTGAATTAGATCTTGGAAATGCTCGACTCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

300

Amino Acids

33.32

Weight (kDa)

10.27

Isoelectric Point (pI)

53.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07749 ATMG00490 ATMG01110 ATMG01410
pyrus_communis pycom12401g00030 pycom461g00360
rosa_chinensis RchiOBHm_MTg0498601
rosa_laevigata RLG00000000451
rosa_multiflora Rmu_co8289347.1_g000001
rosa_roxburghii Rroxscaffold_2G00119420 Rroxscaffold_5G00338830
rosa_samantha Rh4AG240200 Rh4BG243400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 309
AccB1I GGYRCC 1 cut(s) 309
AccII CGCG 3 cut(s) 329, 342, 531
AciI CCGC 5 cut(s) 327, 531, 546, 596, 725
AclWI GGATC 5 cut(s) 492, 566, 579, 695, 708
AcoI YGGCCR 2 cut(s) 627, 684
AcsI RAATTY 1 cut(s) 768
AcuI CTGAAG 1 cut(s) 813
AfaI GTAC 4 cut(s) 24, 134, 168, 311
AflII CTTAAG 2 cut(s) 32, 815
AgsI TTSAA 3 cut(s) 217, 455, 869
AjnI CCWGG 3 cut(s) 591, 720, 781
AjuI GAANNNNNNNTTGG 2 cut(s) 861, 893
AleI CACNNNNGTG 1 cut(s) 43
AloI GAACNNNNNNTCC 2 cut(s) 794, 826
AluBI AGCT 2 cut(s) 174, 509
AluI AGCT 2 cut(s) 174, 509
AlwI GGATC 5 cut(s) 492, 566, 579, 695, 708
AlwNI CAGNNNCTG 1 cut(s) 788
Ama87I CYCGRG 1 cut(s) 363
AoxI GGCC 2 cut(s) 627, 684
ApeKI GCWGC 1 cut(s) 599
ApoI RAATTY 1 cut(s) 768
Asp700I GAANNNNTTC 3 cut(s) 66, 378, 547
Asp718I GGTACC 1 cut(s) 309
AspS9I GGNCC 4 cut(s) 97, 359, 778, 785
AsuHPI GGTGA 4 cut(s) 140, 543, 615, 672
AvaI CYCGRG 1 cut(s) 363
AvaII GGWCC 4 cut(s) 97, 359, 778, 785
BaeI ACNNNNGTAYC 2 cut(s) 301, 334
BamHI GGATCC 2 cut(s) 571, 700
BanI GGYRCC 1 cut(s) 309
BbvI GCAGC 1 cut(s) 586
BccI CCATC 1 cut(s) 509
BciT130I CCWGG 3 cut(s) 593, 722, 783
BclI TGATCA 2 cut(s) 612, 669
BfmI CTRYAG 1 cut(s) 41
BfrI CTTAAG 2 cut(s) 32, 815
BglII AGATCT 2 cut(s) 64, 874
BisI GCNGC 3 cut(s) 597, 600, 726
BlsI GCNGC 3 cut(s) 598, 601, 727
BmcAI AGTACT 2 cut(s) 134, 168
Bme1390I CCNGG 3 cut(s) 593, 722, 783
Bme18I GGWCC 4 cut(s) 97, 359, 778, 785
BmeT110I CYCGRG 1 cut(s) 363
BmgT120I GGNCC 4 cut(s) 97, 359, 778, 785
BmiI GGNNCC 5 cut(s) 311, 573, 702, 779, 780
BmrFI CCNGG 3 cut(s) 593, 722, 783
BmsI GCATC 2 cut(s) 84, 800
Bsa29I ATCGAT 1 cut(s) 638
BsaBI GATNNNNATC 2 cut(s) 419, 483
BsaJI CCNNGG 2 cut(s) 362, 781
BsaWI WCCGGW 1 cut(s) 745
Bse118I RCCGGY 2 cut(s) 625, 682
Bse1I ACTGG 1 cut(s) 287
Bse8I GATNNNNATC 2 cut(s) 419, 483
BseBI CCWGG 3 cut(s) 593, 722, 783
BseCI ATCGAT 1 cut(s) 638
BseDI CCNNGG 2 cut(s) 362, 781
BseGI GGATG 2 cut(s) 501, 791
BseJI GATNNNNATC 2 cut(s) 419, 483
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 1 cut(s) 287
BseXI GCAGC 1 cut(s) 586
Bsh1236I CGCG 3 cut(s) 329, 342, 531
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 2 cut(s) 629, 686
BshNI GGYRCC 1 cut(s) 309
BshVI ATCGAT 1 cut(s) 638
BsiEI CGRYCG 1 cut(s) 259
BsiHKCI CYCGRG 1 cut(s) 363
BsiSI CCGG 3 cut(s) 626, 683, 746
BslFI GGGAC 1 cut(s) 764
BsmFI GGGAC 1 cut(s) 764
BsmI GAATGC 1 cut(s) 380
BsnI GGCC 2 cut(s) 629, 686
BsoBI CYCGRG 1 cut(s) 363
BspACI CCGC 5 cut(s) 327, 531, 546, 596, 725
BspANI GGCC 2 cut(s) 629, 686
BspCNI CTCAG 1 cut(s) 162
BspDI ATCGAT 1 cut(s) 638
BspFNI CGCG 3 cut(s) 329, 342, 531
BspLI GGNNCC 5 cut(s) 311, 573, 702, 779, 780
BspPI GGATC 5 cut(s) 492, 566, 579, 695, 708
BspT107I GGYRCC 1 cut(s) 309
BspTI CTTAAG 2 cut(s) 32, 815
BsrFI RCCGGY 2 cut(s) 625, 682
BsrI ACTGG 1 cut(s) 287
BssAI RCCGGY 2 cut(s) 625, 682
BssECI CCNNGG 2 cut(s) 362, 781
Bst2UI CCWGG 3 cut(s) 593, 722, 783
Bst4CI ACNGT 6 cut(s) 9, 45, 119, 137, 353, 459
Bst6I CTCTTC 1 cut(s) 157
BstAFI CTTAAG 2 cut(s) 32, 815
BstC8I GCNNGC 1 cut(s) 604
BstDEI CTNAG 5 cut(s) 93, 126, 170, 175, 494
BstF5I GGATG 2 cut(s) 501, 791
BstFNI CGCG 3 cut(s) 329, 342, 531
BstMCI CGRYCG 1 cut(s) 259
BstMWI GCNNNNNNNGC 1 cut(s) 759
BstNI CCWGG 3 cut(s) 593, 722, 783
BstSCI CCNGG 3 cut(s) 591, 720, 781
BstSFI CTRYAG 1 cut(s) 41
BstUI CGCG 3 cut(s) 329, 342, 531
BstV1I GCAGC 1 cut(s) 586
BstX2I RGATCY 4 cut(s) 64, 571, 700, 874
BstXI CCANNNNNNTGG 1 cut(s) 45
BstYI RGATCY 4 cut(s) 64, 571, 700, 874
Bsu15I ATCGAT 1 cut(s) 638
BsuRI GGCC 2 cut(s) 629, 686
BsuTUI ATCGAT 1 cut(s) 638
BtsCI GGATG 2 cut(s) 501, 791
BtsI GCAGTG 2 cut(s) 201, 227
BtsIMutI CAGTG 6 cut(s) 50, 201, 227, 233, 622, 679
Cac8I GCNNGC 1 cut(s) 604
CaiI CAGNNNCTG 1 cut(s) 788
Cfr10I RCCGGY 2 cut(s) 625, 682
Cfr13I GGNCC 4 cut(s) 97, 359, 778, 785
ClaI ATCGAT 1 cut(s) 638
Csp6I GTAC 4 cut(s) 23, 133, 167, 310
CviQI GTAC 4 cut(s) 23, 133, 167, 310
DdeI CTNAG 5 cut(s) 93, 126, 170, 175, 494
EaeI YGGCCR 2 cut(s) 627, 684
Eam1104I CTCTTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 157
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 4 cut(s) 97, 359, 778, 785
Eco57I CTGAAG 1 cut(s) 813
Eco88I CYCGRG 1 cut(s) 363
EcoO109I RGGNCCY 2 cut(s) 778, 785
EcoRII CCWGG 3 cut(s) 591, 720, 781
EcoRV GATATC 1 cut(s) 438
FaiI YATR 7 cut(s) 123, 211, 249, 320, 482, 764, 855
FaqI GGGAC 1 cut(s) 764
FauI CCCGC 1 cut(s) 334
FbaI TGATCA 2 cut(s) 612, 669
Fnu4HI GCNGC 3 cut(s) 597, 600, 726
FokI GGATG 2 cut(s) 488, 778
Fsp4HI GCNGC 3 cut(s) 597, 600, 726
GluI GCNGC 3 cut(s) 597, 600, 726
HaeIII GGCC 2 cut(s) 629, 686
HapII CCGG 3 cut(s) 626, 683, 746
HinfI GANTC 7 cut(s) 85, 298, 523, 620, 646, 677, 891
HpaII CCGG 3 cut(s) 626, 683, 746
HphI GGTGA 4 cut(s) 140, 543, 615, 672
Hpy166II GTNNAC 3 cut(s) 201, 266, 551
Hpy188I TCNGA 6 cut(s) 29, 297, 414, 471, 522, 832
Hpy188III TCNNGA 2 cut(s) 189, 263
Hpy8I GTNNAC 3 cut(s) 201, 266, 551
Hpy99I CGWCG 3 cut(s) 119, 264, 306
HpyCH4III ACNGT 6 cut(s) 9, 45, 119, 137, 353, 459
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 3 cut(s) 428, 762, 791
HpyF10VI GCNNNNNNNGC 1 cut(s) 759
HpyF3I CTNAG 5 cut(s) 93, 126, 170, 175, 494
HpySE526I ACGT 1 cut(s) 184
KflI GGGWCCC 1 cut(s) 778
KpnI GGTACC 1 cut(s) 313
Ksp22I TGATCA 2 cut(s) 612, 669
Lsp1109I GCAGC 1 cut(s) 586
LweI GCATC 2 cut(s) 84, 800
MaeII ACGT 1 cut(s) 184
MfeI CAATTG 1 cut(s) 101
MflI RGATCY 4 cut(s) 64, 571, 700, 874
MluCI AATT 4 cut(s) 101, 321, 768, 869
MlyI GAGTC 2 cut(s) 292, 885
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 3 cut(s) 299, 324, 372
MroXI GAANNNNTTC 3 cut(s) 66, 378, 547
MseI TTAA 4 cut(s) 33, 474, 587, 816
MslI CAYNNNNRTG 1 cut(s) 43
MspCI CTTAAG 2 cut(s) 32, 815
MspI CCGG 3 cut(s) 626, 683, 746
MspR9I CCNGG 3 cut(s) 593, 722, 783
MunI CAATTG 1 cut(s) 101
Mva1269I GAATGC 1 cut(s) 380
MvaI CCWGG 3 cut(s) 593, 722, 783
MvnI CGCG 3 cut(s) 329, 342, 531
MwoI GCNNNNNNNGC 1 cut(s) 759
NlaIV GGNNCC 5 cut(s) 311, 573, 702, 779, 780
OliI CACNNNNGTG 1 cut(s) 43
PctI GAATGC 1 cut(s) 380
PdmI GAANNNNTTC 3 cut(s) 66, 378, 547
PfeI GAWTC 5 cut(s) 85, 523, 620, 646, 677
PkrI GCNGC 3 cut(s) 598, 601, 727
Ple19I CGATCG 1 cut(s) 259
PleI GAGTC 2 cut(s) 292, 885
PpsI GAGTC 2 cut(s) 292, 885
PpuMI RGGWCCY 2 cut(s) 778, 785
Psp5II RGGWCCY 2 cut(s) 778, 785
Psp6I CCWGG 3 cut(s) 591, 720, 781
PspGI CCWGG 3 cut(s) 591, 720, 781
PspN4I GGNNCC 5 cut(s) 311, 573, 702, 779, 780
PspPI GGNCC 4 cut(s) 97, 359, 778, 785
PspPPI RGGWCCY 2 cut(s) 778, 785
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 4 cut(s) 64, 571, 700, 874
PvuI CGATCG 1 cut(s) 259
RsaI GTAC 4 cut(s) 24, 134, 168, 311
RsaNI GTAC 4 cut(s) 23, 133, 167, 310
RseI CAYNNNNRTG 1 cut(s) 43
SaqAI TTAA 4 cut(s) 33, 474, 587, 816
SatI GCNGC 3 cut(s) 597, 600, 726
Sau96I GGNCC 4 cut(s) 97, 359, 778, 785
ScaI AGTACT 2 cut(s) 134, 168
SchI GAGTC 2 cut(s) 292, 885
ScrFI CCNGG 3 cut(s) 593, 722, 783
SfaNI GCATC 2 cut(s) 84, 800
SfcI CTRYAG 1 cut(s) 41
SinI GGWCC 4 cut(s) 97, 359, 778, 785
SmiMI CAYNNNNRTG 1 cut(s) 43
SmlI CTYRAG 2 cut(s) 32, 815
SmoI CTYRAG 2 cut(s) 32, 815
Sse9I AATT 4 cut(s) 101, 321, 768, 869
SsiI CCGC 5 cut(s) 327, 531, 546, 596, 725
StyD4I CCNGG 3 cut(s) 591, 720, 781
TaaI ACNGT 6 cut(s) 9, 45, 119, 137, 353, 459
TaiI ACGT 1 cut(s) 187
TaqI TCGA 6 cut(s) 15, 114, 160, 304, 638, 889
TasI AATT 4 cut(s) 101, 321, 768, 869
TatI WGTACW 3 cut(s) 22, 132, 166
TauI GCSGC 2 cut(s) 599, 728
TfiI GAWTC 5 cut(s) 85, 523, 620, 646, 677
Tru1I TTAA 4 cut(s) 33, 474, 587, 816
Tru9I TTAA 4 cut(s) 33, 474, 587, 816
TscAI CASTG 6 cut(s) 50, 208, 227, 240, 622, 679
TseI GCWGC 1 cut(s) 599
TspDTI ATGAA 4 cut(s) 216, 387, 451, 656
TspRI CASTG 6 cut(s) 50, 208, 227, 240, 622, 679
Vha464I CTTAAG 2 cut(s) 32, 815
VpaK11BI GGWCC 4 cut(s) 97, 359, 778, 785
XapI RAATTY 1 cut(s) 768
XcmI CCANNNNNNNNNTGG 1 cut(s) 565
XmnI GAANNNNTTC 3 cut(s) 66, 378, 547
ZrmI AGTACT 2 cut(s) 134, 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.