RLG00000000451

Mitovirus RNA-dependent RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017129
Physical Location & Seq
Forward (+)
1018324 .. 1019753
1430 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000451

Sequence Viewer

Length: 837 bp
ATGCGAAATGGCGGGGATCCTAATAAACCGACTCTTTTACCGGTTCCTATATCTTTGACTCGTCGGGGGCTCCCGCGGACCATTCCACCTTTCTATAGGTCGATGATCCGATCTGGAGGTCCGAAGGTGGTGAAATGTTACTTATCTTTCTTCTCTTTGTATAGGATCATTCTCCTAGCAAAACTAATTGATAAGACAACATTTTCATCGATTGTGACTCCTGTTCAGGACATGCATCGTGTCTATTCAACGATATCACGGATGAAGGAAAGTGTGGTGCCCTTGTTAGACCGGTACATCCCATGGTTTCGACGCATACTATTATACCAAGGTATGTCGTGGGAACCAACCTGGAAGACCGTTCCAACTATGTCACAGGCTAACGAGAAGCTTAAGTCAGGAGCCTCTCAACTTGGGCTGGGCCCAAGTTTCATGGACCTTATAAGTCCTCCACTGGGGTACTACTCCTCTTGGCCTCTGTTCGCACTCACTCATCACTTTGTGGTGTGGTGGTGTGCGCAGCAGGTTCATCCTGGTCGACGCTTTGATAAGTACGCAGTGCTTGGCGATGACGTCATAATTTGTGACGATCTCGTCGCTGAGAAATACAGGGAGGTCCTGGATTTCCTTGAGGCCAAGATTTCTGTACATAAATCGTTGATCTCGCATTCTGGGACTGGTGAGTTTGCTAAGAGATTTCGTGTTCGGGGTCTGGCGGTCGATTTGTCGCCGGCCTCAGCACAAGCCCTAACTAACTTTTATCATCCTTATGGCCTTATAGCTATAAATGATAGGTATCCGGTACGCAGGGTTTGGTTTCAACCCTGTGCAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mitovir_RNA_pol

Protein Analysis

279

Amino Acids

31.73

Weight (kDa)

9.8

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mitovir_RNA_pol PF05919 151 - 257 4.3e-30 Mitovirus RNA-dependent RNA polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07749 ATMG00490 ATMG01110 ATMG01410
pyrus_communis pycom12401g00030 pycom461g00360
rosa_chinensis RchiOBHm_MTg0498601
rosa_laevigata RLG00000000451
rosa_multiflora Rmu_co8289347.1_g000001
rosa_roxburghii Rroxscaffold_2G00119420 Rroxscaffold_5G00338830
rosa_samantha Rh4AG240200 Rh4BG243400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 443
AasI GACNNNNNNGTC 1 cut(s) 593
AatII GACGTC 1 cut(s) 576
Acc16I TGCGCA 1 cut(s) 519
Acc36I ACCTGC 1 cut(s) 514
AccB1I GGYRCC 1 cut(s) 277
AccI GTMKAC 1 cut(s) 538
AccII CGCG 1 cut(s) 76
AciI CCGC 4 cut(s) 12, 74, 76, 716
AclWI GGATC 4 cut(s) 11, 24, 100, 173
AcyI GRCGYC 1 cut(s) 573
AdeI CACNNNGTG 1 cut(s) 502
AfaI GTAC 5 cut(s) 296, 461, 554, 648, 804
AfiI CCNNNNNNNGG 2 cut(s) 455, 830
AflII CTTAAG 1 cut(s) 392
AgeI ACCGGT 2 cut(s) 40, 291
AgsI TTSAA 2 cut(s) 249, 821
AjnI CCWGG 3 cut(s) 350, 532, 618
AluBI AGCT 2 cut(s) 391, 782
AluI AGCT 2 cut(s) 391, 782
AlwI GGATC 4 cut(s) 11, 24, 100, 173
AoxI GGCC 5 cut(s) 421, 473, 633, 732, 772
ApaI GGGCCC 1 cut(s) 425
ApeKI GCWGC 1 cut(s) 520
AsiGI ACCGGT 2 cut(s) 40, 291
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 6 cut(s) 78, 119, 421, 422, 436, 616
AsuHPI GGTGA 2 cut(s) 142, 692
AvaII GGWCC 4 cut(s) 78, 119, 436, 616
BaeGI GKGCMC 2 cut(s) 282, 425
BamHI GGATCC 1 cut(s) 16
BanI GGYRCC 1 cut(s) 277
BanII GRGCYC 2 cut(s) 72, 425
BbsI GAAGAC 1 cut(s) 362
BbvCI CCTCAGC 1 cut(s) 736
BbvI GCAGC 1 cut(s) 532
BciT130I CCWGG 3 cut(s) 352, 534, 620
BciVI GTATCC 1 cut(s) 807
BfaI CTAG 1 cut(s) 176
BfmI CTRYAG 1 cut(s) 94
BfrI CTTAAG 1 cut(s) 392
BfuAI ACCTGC 1 cut(s) 514
BfuI GTATCC 1 cut(s) 807
BisI GCNGC 1 cut(s) 521
BlsI GCNGC 1 cut(s) 522
Bme1390I CCNGG 3 cut(s) 352, 534, 620
Bme18I GGWCC 4 cut(s) 78, 119, 436, 616
BmgT120I GGNCC 6 cut(s) 78, 119, 421, 422, 436, 616
BmiI GGNNCC 7 cut(s) 18, 45, 71, 279, 345, 403, 423
BmrFI CCNGG 3 cut(s) 352, 534, 620
BmrI ACTGGG 1 cut(s) 464
BmsI GCATC 1 cut(s) 244
BmuI ACTGGG 1 cut(s) 464
BpiI GAAGAC 1 cut(s) 362
BpmI CTGGAG 1 cut(s) 135
Bpu10I CCTNAGC 1 cut(s) 736
BpuEI CTTGAG 1 cut(s) 650
Bsa29I ATCGAT 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 795
BsaHI GRCGYC 1 cut(s) 573
BsaJI CCNNGG 3 cut(s) 74, 302, 328
BsaWI WCCGGW 3 cut(s) 40, 291, 799
Bsc4I CCNNNNNNNGG 2 cut(s) 455, 830
Bse118I RCCGGY 3 cut(s) 40, 291, 730
Bse1I ACTGG 2 cut(s) 459, 682
Bse8I GATNNNNATC 1 cut(s) 795
BseBI CCWGG 3 cut(s) 352, 534, 620
BseCI ATCGAT 1 cut(s) 209
BseDI CCNNGG 3 cut(s) 74, 302, 328
BseGI GGATG 4 cut(s) 267, 297, 529, 763
BseJI GATNNNNATC 1 cut(s) 795
BseLI CCNNNNNNNGG 2 cut(s) 455, 830
BseMII CTCAG 2 cut(s) 591, 750
BseNI ACTGG 2 cut(s) 459, 682
BseRI GAGGAG 1 cut(s) 457
BseSI GKGCMC 2 cut(s) 282, 425
BseXI GCAGC 1 cut(s) 532
BseYI CCCAGC 1 cut(s) 418
Bsh1236I CGCG 1 cut(s) 76
Bsh1285I CGRYCG 1 cut(s) 720
BshFI GGCC 5 cut(s) 423, 475, 635, 734, 774
BshNI GGYRCC 1 cut(s) 277
BshTI ACCGGT 2 cut(s) 40, 291
BshVI ATCGAT 1 cut(s) 209
BsiEI CGRYCG 1 cut(s) 720
BsiSI CCGG 4 cut(s) 41, 292, 731, 800
BslFI GGGAC 1 cut(s) 688
BslI CCNNNNNNNGG 2 cut(s) 455, 830
BsmFI GGGAC 1 cut(s) 688
BsmI GAATGC 1 cut(s) 667
BsnI GGCC 5 cut(s) 423, 475, 635, 734, 774
Bsp120I GGGCCC 1 cut(s) 421
Bsp1286I GDGCHC 3 cut(s) 72, 282, 425
Bsp1407I TGTACA 1 cut(s) 646
Bsp143I GATC 6 cut(s) 16, 105, 110, 165, 589, 660
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 4 cut(s) 12, 74, 76, 716
BspANI GGCC 5 cut(s) 423, 475, 635, 734, 774
BspCNI CTCAG 2 cut(s) 592, 749
BspDI ATCGAT 1 cut(s) 209
BspFNI CGCG 1 cut(s) 76
BspLI GGNNCC 7 cut(s) 18, 45, 71, 279, 345, 403, 423
BspMI ACCTGC 1 cut(s) 514
BspPI GGATC 4 cut(s) 11, 24, 100, 173
BspT107I GGYRCC 1 cut(s) 277
BspTI CTTAAG 1 cut(s) 392
BsrFI RCCGGY 3 cut(s) 40, 291, 730
BsrGI TGTACA 1 cut(s) 646
BsrI ACTGG 2 cut(s) 459, 682
BssAI RCCGGY 3 cut(s) 40, 291, 730
BssECI CCNNGG 3 cut(s) 74, 302, 328
BssMI GATC 6 cut(s) 16, 105, 110, 165, 589, 660
BssNI GRCGYC 1 cut(s) 573
BssT1I CCWWGG 2 cut(s) 302, 328
Bst2UI CCWGG 3 cut(s) 352, 534, 620
Bst4CI ACNGT 1 cut(s) 361
BstACI GRCGYC 1 cut(s) 573
BstAFI CTTAAG 1 cut(s) 392
BstAUI TGTACA 1 cut(s) 646
BstC8I GCNNGC 1 cut(s) 732
BstDEI CTNAG 3 cut(s) 600, 690, 736
BstDSI CCRYGG 2 cut(s) 74, 302
BstF5I GGATG 4 cut(s) 267, 297, 529, 763
BstFNI CGCG 1 cut(s) 76
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 6 cut(s) 19, 108, 113, 168, 592, 663
BstMBI GATC 6 cut(s) 16, 105, 110, 165, 589, 660
BstMCI CGRYCG 1 cut(s) 720
BstNI CCWGG 3 cut(s) 352, 534, 620
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 3 cut(s) 350, 532, 618
BstSFI CTRYAG 1 cut(s) 94
BstSLI GKGCMC 2 cut(s) 282, 425
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 1 cut(s) 532
BstV2I GAAGAC 1 cut(s) 362
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
Bsu15I ATCGAT 1 cut(s) 209
BsuI GTATCC 1 cut(s) 807
BsuRI GGCC 5 cut(s) 423, 475, 635, 734, 774
BsuTUI ATCGAT 1 cut(s) 209
BtgI CCRYGG 2 cut(s) 74, 302
BtgZI GCGATG 1 cut(s) 582
BtsCI GGATG 4 cut(s) 267, 297, 529, 763
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 2 cut(s) 452, 564
BveI ACCTGC 1 cut(s) 514
Cac8I GCNNGC 1 cut(s) 732
CfoI GCGC 1 cut(s) 520
Cfr10I RCCGGY 3 cut(s) 40, 291, 730
Cfr13I GGNCC 6 cut(s) 78, 119, 421, 422, 436, 616
Cfr42I CCGCGG 1 cut(s) 77
ClaI ATCGAT 1 cut(s) 209
CseI GACGC 2 cut(s) 321, 549
Csp6I GTAC 5 cut(s) 295, 460, 553, 647, 803
CspAI ACCGGT 2 cut(s) 40, 291
CviAII CATG 3 cut(s) 232, 303, 433
CviQI GTAC 5 cut(s) 295, 460, 553, 647, 803
DdeI CTNAG 3 cut(s) 600, 690, 736
DpnI GATC 6 cut(s) 18, 107, 112, 167, 591, 662
DpnII GATC 6 cut(s) 16, 105, 110, 165, 589, 660
DraIII CACNNNGTG 1 cut(s) 502
DrdI GACNNNNNNGTC 1 cut(s) 593
DseDI GACNNNNNNGTC 1 cut(s) 593
Eco130I CCWWGG 2 cut(s) 302, 328
Eco24I GRGCYC 2 cut(s) 72, 425
Eco32I GATATC 1 cut(s) 255
Eco47I GGWCC 4 cut(s) 78, 119, 436, 616
EcoO109I RGGNCCY 1 cut(s) 616
EcoRII CCWGG 3 cut(s) 350, 532, 618
EcoRV GATATC 1 cut(s) 255
EcoT14I CCWWGG 2 cut(s) 302, 328
EcoT22I ATGCAT 1 cut(s) 237
EcoT38I GRGCYC 2 cut(s) 72, 425
ErhI CCWWGG 2 cut(s) 302, 328
FaeI CATG 3 cut(s) 235, 306, 436
FaqI GGGAC 1 cut(s) 688
FatI CATG 3 cut(s) 231, 302, 432
FauI CCCGC 2 cut(s) 5, 81
FblI GTMKAC 1 cut(s) 538
Fnu4HI GCNGC 1 cut(s) 521
FokI GGATG 4 cut(s) 274, 284, 516, 750
FriOI GRGCYC 2 cut(s) 72, 425
Fsp4HI GCNGC 1 cut(s) 521
FspBI CTAG 1 cut(s) 176
FspI TGCGCA 1 cut(s) 519
GlaI GCGC 1 cut(s) 519
GluI GCNGC 1 cut(s) 521
GsaI CCCAGC 1 cut(s) 422
GsuI CTGGAG 1 cut(s) 135
HaeIII GGCC 5 cut(s) 423, 475, 635, 734, 774
HapII CCGG 4 cut(s) 41, 292, 731, 800
HgaI GACGC 2 cut(s) 321, 549
HhaI GCGC 1 cut(s) 520
Hin1I GRCGYC 1 cut(s) 573
Hin1II CATG 3 cut(s) 235, 306, 436
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 1 cut(s) 539
HindII GTYRAC 1 cut(s) 539
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 3 cut(s) 31, 58, 217
HpaII CCGG 4 cut(s) 41, 292, 731, 800
HphI GGTGA 2 cut(s) 142, 692
Hpy166II GTNNAC 1 cut(s) 539
Hpy188I TCNGA 2 cut(s) 110, 123
Hpy188III TCNNGA 3 cut(s) 114, 227, 399
Hpy8I GTNNAC 1 cut(s) 539
Hpy99I CGWCG 4 cut(s) 66, 315, 543, 599
HpyAV CCTTC 2 cut(s) 118, 259
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 1 cut(s) 573
HpyCH4V TGCA 2 cut(s) 235, 830
HpyF3I CTNAG 3 cut(s) 600, 690, 736
HpySE526I ACGT 1 cut(s) 573
Hsp92I GRCGYC 1 cut(s) 573
Hsp92II CATG 3 cut(s) 235, 306, 436
HspAI GCGC 1 cut(s) 518
KroI GCCGGC 1 cut(s) 730
KroNI GCCGGC 1 cut(s) 732
KspI CCGCGG 1 cut(s) 77
Kzo9I GATC 6 cut(s) 16, 105, 110, 165, 589, 660
LmnI GCTCC 2 cut(s) 75, 401
Lsp1109I GCAGC 1 cut(s) 532
LweI GCATC 1 cut(s) 244
MaeI CTAG 1 cut(s) 176
MaeII ACGT 1 cut(s) 573
MaeIII GTNAC 4 cut(s) 137, 214, 372, 584
MalI GATC 6 cut(s) 18, 107, 112, 167, 591, 662
MboI GATC 6 cut(s) 16, 105, 110, 165, 589, 660
MboII GAAGA 2 cut(s) 142, 367
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 3 cut(s) 72, 282, 425
MluCI AATT 2 cut(s) 186, 579
MlyI GAGTC 3 cut(s) 25, 52, 211
MmeI TCCRAC 1 cut(s) 389
MnlI CCTC 8 cut(s) 110, 415, 459, 478, 486, 607, 625, 745
Mph1103I ATGCAT 1 cut(s) 237
MroNI GCCGGC 1 cut(s) 730
MseI TTAA 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 768
MspA1I CMGCKG 1 cut(s) 76
MspCI CTTAAG 1 cut(s) 392
MspI CCGG 4 cut(s) 41, 292, 731, 800
MspR9I CCNGG 3 cut(s) 352, 534, 620
Mva1269I GAATGC 1 cut(s) 667
MvaI CCWGG 3 cut(s) 352, 534, 620
MvnI CGCG 1 cut(s) 76
NaeI GCCGGC 1 cut(s) 732
NcoI CCATGG 1 cut(s) 302
NdeII GATC 6 cut(s) 16, 105, 110, 165, 589, 660
NgoMIV GCCGGC 1 cut(s) 730
NlaIII CATG 3 cut(s) 235, 306, 436
NlaIV GGNNCC 7 cut(s) 18, 45, 71, 279, 345, 403, 423
NmuCI GTSAC 3 cut(s) 214, 372, 584
NsbI TGCGCA 1 cut(s) 519
NsiI ATGCAT 1 cut(s) 237
NspI RCATGY 1 cut(s) 235
PctI GAATGC 1 cut(s) 667
PdiI GCCGGC 1 cut(s) 732
PfoI TCCNGGA 1 cut(s) 618
PinAI ACCGGT 2 cut(s) 40, 291
PkrI GCNGC 1 cut(s) 522
PleI GAGTC 3 cut(s) 25, 52, 211
PpsI GAGTC 3 cut(s) 25, 52, 211
PpuMI RGGWCCY 1 cut(s) 616
PsiI TTATAA 1 cut(s) 443
Psp5II RGGWCCY 1 cut(s) 616
Psp6I CCWGG 3 cut(s) 350, 532, 618
PspFI CCCAGC 1 cut(s) 418
PspGI CCWGG 3 cut(s) 350, 532, 618
PspN4I GGNNCC 7 cut(s) 18, 45, 71, 279, 345, 403, 423
PspOMI GGGCCC 1 cut(s) 421
PspPI GGNCC 6 cut(s) 78, 119, 421, 422, 436, 616
PspPPI RGGWCCY 1 cut(s) 616
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 5 cut(s) 296, 461, 554, 648, 804
RsaNI GTAC 5 cut(s) 295, 460, 553, 647, 803
RseI CAYNNNNRTG 1 cut(s) 768
SacII CCGCGG 1 cut(s) 77
SalI GTCGAC 1 cut(s) 537
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 1 cut(s) 521
Sau3AI GATC 6 cut(s) 16, 105, 110, 165, 589, 660
Sau96I GGNCC 6 cut(s) 78, 119, 421, 422, 436, 616
SchI GAGTC 3 cut(s) 25, 52, 211
ScrFI CCNGG 3 cut(s) 352, 534, 620
SduI GDGCHC 3 cut(s) 72, 282, 425
SfaNI GCATC 1 cut(s) 244
SfcI CTRYAG 1 cut(s) 94
Sfr303I CCGCGG 1 cut(s) 77
SgrBI CCGCGG 1 cut(s) 77
SinI GGWCC 4 cut(s) 78, 119, 436, 616
SmiMI CAYNNNNRTG 1 cut(s) 768
SmlI CTYRAG 2 cut(s) 392, 629
SmoI CTYRAG 2 cut(s) 392, 629
Sse9I AATT 2 cut(s) 186, 579
SsiI CCGC 4 cut(s) 12, 74, 76, 716
SspMI CTAG 1 cut(s) 176
StyD4I CCNGG 3 cut(s) 350, 532, 618
StyI CCWWGG 2 cut(s) 302, 328
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 1 cut(s) 576
TaqI TCGA 5 cut(s) 101, 209, 310, 538, 720
TasI AATT 2 cut(s) 186, 579
TatI WGTACW 1 cut(s) 646
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 2 cut(s) 459, 564
TseFI GTSAC 3 cut(s) 214, 372, 584
TseI GCWGC 1 cut(s) 520
Tsp45I GTSAC 3 cut(s) 214, 372, 584
TspDTI ATGAA 4 cut(s) 195, 278, 421, 518
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 2 cut(s) 459, 564
Vha464I CTTAAG 1 cut(s) 392
VpaK11BI GGWCC 4 cut(s) 78, 119, 436, 616
XceI RCATGY 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 538
XspI CTAG 1 cut(s) 176
ZraI GACGTC 1 cut(s) 574
Zsp2I ATGCAT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.