Rroxscaffold_5G00338830

BEST Arabidopsis thaliana protein match is Mitovirus RNA-dependent RNA polymerase (TAIR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017129
Physical Location & Seq
Reverse (-)
7104847 .. 7105330
484 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00338830.1

Sequence Viewer

Length: 291 bp
ATGGATCGGCTTCGAAGGTCTTGCGACCTTTTTGCGGACGGTACCTTTAACCAGCAGCGACCTCTTGATCGTCTAATTGGCCACCGGGGTACTACTCCTCTTGGCCTCTCGTTCGCACTCACTCATCACTTTGTGGTGTGGTGGTGTGCGCAGCGAGTTCATCCCGGTCGACGCTTTGATAAGTACGCGGTGCTTGGCGATGACGTCATAATTTGTGACGATCTCGTCGCCGAGAAATACAAGGAGGTCCTGGATTTCCTTGAGGCCAAGATTTCGTACATAAATCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mitovir_RNA_pol

Protein Analysis

96

Amino Acids

11.21

Weight (kDa)

7.77

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mitovir_RNA_pol PF05919 38 - 92 8.1e-17 Mitovirus RNA-dependent RNA polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07749 ATMG00490 ATMG01110 ATMG01410
pyrus_communis pycom12401g00030 pycom461g00360
rosa_chinensis RchiOBHm_MTg0498601
rosa_laevigata RLG00000000451
rosa_multiflora Rmu_co8289347.1_g000001
rosa_roxburghii Rroxscaffold_2G00119420 Rroxscaffold_5G00338830
rosa_samantha Rh4AG240200 Rh4BG243400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
AatII GACGTC 1 cut(s) 207
Acc16I TGCGCA 1 cut(s) 150
Acc65I GGTACC 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 169
AccII CGCG 1 cut(s) 188
AciI CCGC 2 cut(s) 35, 188
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 79
AcyI GRCGYC 1 cut(s) 204
AdeI CACNNNGTG 1 cut(s) 133
AfaI GTAC 4 cut(s) 43, 91, 185, 278
AfiI CCNNNNNNNGG 1 cut(s) 34
AjnI CCWGG 1 cut(s) 249
AlwI GGATC 1 cut(s) 12
AoxI GGCC 3 cut(s) 79, 103, 264
ApeKI GCWGC 2 cut(s) 55, 151
Asp718I GGTACC 1 cut(s) 41
AspLEI GCGC 1 cut(s) 151
AspS9I GGNCC 1 cut(s) 247
AsuC2I CCSGG 2 cut(s) 86, 165
AsuII TTCGAA 1 cut(s) 13
AvaII GGWCC 1 cut(s) 247
BalI TGGCCA 1 cut(s) 81
BanI GGYRCC 1 cut(s) 41
BbvI GCAGC 2 cut(s) 67, 163
BcgI CGANNNNNNTGC 3 cut(s) 14, 37, 48
BciT130I CCWGG 1 cut(s) 251
BcnI CCSGG 2 cut(s) 86, 165
BisI GCNGC 2 cut(s) 56, 152
BlsI GCNGC 2 cut(s) 57, 153
Bme1390I CCNGG 3 cut(s) 86, 165, 251
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 3 cut(s) 86, 165, 251
Bpu14I TTCGAA 1 cut(s) 13
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 2 cut(s) 86, 165
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 85
Bsc4I CCNNNNNNNGG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 1 cut(s) 85
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 1 cut(s) 34
BseRI GAGGAG 1 cut(s) 87
BseXI GCAGC 2 cut(s) 67, 163
Bsh1236I CGCG 1 cut(s) 188
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 3 cut(s) 81, 105, 266
BshNI GGYRCC 1 cut(s) 41
BsiEI CGRYCG 1 cut(s) 169
BsiSI CCGG 2 cut(s) 85, 165
BslI CCNNNNNNNGG 1 cut(s) 34
BsnI GGCC 3 cut(s) 81, 105, 266
Bsp119I TTCGAA 1 cut(s) 13
Bsp143I GATC 3 cut(s) 4, 67, 220
BspACI CCGC 2 cut(s) 35, 188
BspANI GGCC 3 cut(s) 81, 105, 266
BspFNI CGCG 1 cut(s) 188
BspLI GGNNCC 1 cut(s) 43
BspPI GGATC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 13
BspT107I GGYRCC 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 3 cut(s) 4, 67, 220
BssNI GRCGYC 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 13
BstF5I GGATG 1 cut(s) 160
BstFNI CGCG 1 cut(s) 188
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 3 cut(s) 7, 70, 223
BstMBI GATC 3 cut(s) 4, 67, 220
BstMCI CGRYCG 1 cut(s) 169
BstNI CCWGG 1 cut(s) 251
BstSCI CCNGG 3 cut(s) 84, 163, 249
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 2 cut(s) 67, 163
BsuRI GGCC 3 cut(s) 81, 105, 266
BtgZI GCGATG 1 cut(s) 213
BtsCI GGATG 1 cut(s) 160
CfoI GCGC 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 247
CseI GACGC 1 cut(s) 180
Csp6I GTAC 4 cut(s) 42, 90, 184, 277
CviJI RGCY 4 cut(s) 10, 81, 105, 266
CviKI_1 RGCY 4 cut(s) 10, 81, 105, 266
CviQI GTAC 4 cut(s) 42, 90, 184, 277
DpnI GATC 3 cut(s) 6, 69, 222
DpnII GATC 3 cut(s) 4, 67, 220
DraIII CACNNNGTG 1 cut(s) 133
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 249
FaiI YATR 2 cut(s) 209, 281
FblI GTMKAC 1 cut(s) 169
Fnu4HI GCNGC 2 cut(s) 56, 152
FokI GGATG 1 cut(s) 147
Fsp4HI GCNGC 2 cut(s) 56, 152
FspI TGCGCA 1 cut(s) 150
GlaI GCGC 1 cut(s) 150
GluI GCNGC 2 cut(s) 56, 152
HaeIII GGCC 3 cut(s) 81, 105, 266
HapII CCGG 2 cut(s) 85, 165
HgaI GACGC 1 cut(s) 180
HhaI GCGC 1 cut(s) 151
Hin1I GRCGYC 1 cut(s) 204
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HincII GTYRAC 1 cut(s) 170
HindII GTYRAC 1 cut(s) 170
HpaII CCGG 2 cut(s) 85, 165
Hpy166II GTNNAC 1 cut(s) 170
Hpy188III TCNNGA 1 cut(s) 65
Hpy8I GTNNAC 1 cut(s) 170
Hpy99I CGWCG 2 cut(s) 174, 230
HpyAV CCTTC 1 cut(s) 9
HpyCH4III ACNGT 1 cut(s) 41
HpyCH4IV ACGT 1 cut(s) 204
HpySE526I ACGT 1 cut(s) 204
Hsp92I GRCGYC 1 cut(s) 204
HspAI GCGC 1 cut(s) 149
KpnI GGTACC 1 cut(s) 45
Kzo9I GATC 3 cut(s) 4, 67, 220
LpnPI CCDG 5 cut(s) 65, 98, 178, 236, 263
Lsp1109I GCAGC 2 cut(s) 67, 163
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 215
MalI GATC 3 cut(s) 6, 69, 222
MboI GATC 3 cut(s) 4, 67, 220
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 2 cut(s) 75, 210
MluNI TGGCCA 1 cut(s) 81
MnlI CCTC 5 cut(s) 72, 108, 116, 238, 256
Mox20I TGGCCA 1 cut(s) 81
MscI TGGCCA 1 cut(s) 81
MseI TTAA 1 cut(s) 48
Msp20I TGGCCA 1 cut(s) 81
MspI CCGG 2 cut(s) 85, 165
MspR9I CCNGG 3 cut(s) 86, 165, 251
MvaI CCWGG 1 cut(s) 251
MvnI CGCG 1 cut(s) 188
NciI CCSGG 2 cut(s) 86, 165
NdeII GATC 3 cut(s) 4, 67, 220
NlaIV GGNNCC 1 cut(s) 43
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 215
NsbI TGCGCA 1 cut(s) 150
NspV TTCGAA 1 cut(s) 13
PfoI TCCNGGA 1 cut(s) 249
PkrI GCNGC 2 cut(s) 57, 153
PpuMI RGGWCCY 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 249
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 1 cut(s) 43
PspPI GGNCC 1 cut(s) 247
PspPPI RGGWCCY 1 cut(s) 247
RsaI GTAC 4 cut(s) 43, 91, 185, 278
RsaNI GTAC 4 cut(s) 42, 90, 184, 277
SalI GTCGAC 1 cut(s) 168
SaqAI TTAA 1 cut(s) 48
SatI GCNGC 2 cut(s) 56, 152
Sau3AI GATC 3 cut(s) 4, 67, 220
Sau96I GGNCC 1 cut(s) 247
ScrFI CCNGG 3 cut(s) 86, 165, 251
SetI ASST 6 cut(s) 20, 30, 47, 64, 207, 249
SfuI TTCGAA 1 cut(s) 13
SinI GGWCC 1 cut(s) 247
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 2 cut(s) 75, 210
SsiI CCGC 2 cut(s) 35, 188
StyD4I CCNGG 3 cut(s) 84, 163, 249
TaaI ACNGT 1 cut(s) 41
TaiI ACGT 1 cut(s) 207
TaqI TCGA 2 cut(s) 13, 169
TasI AATT 2 cut(s) 75, 210
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 2 cut(s) 55, 151
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 247
XmiI GTMKAC 1 cut(s) 169
ZraI GACGTC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.