RchiOBHm_Chr7g0204931

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0010832
Physical Location & Seq
Forward (+)
22600280 .. 22601786
1507 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 273 bp
ATGCACAGATCCGCGAGTGCTCCTCGAGTCTCCGGGGACTTCCCGGTCAACCCGTCATCAACTTCACCGCAGCAACAACAGCAATCTTCAACCACCACGACCCACAACTCAGAGCACCACCACGATCAAGATCTTCCCACATACGATCCCCTCTCTCATGCCGCCAAGAGAGAACGGTCTCGCATCAAGTCCGCAGAGAACGCCATCCACTTAATACCTCTCGTCCTCGCTCTCTGTGCTATCATCCTCTGGTTCTTCTCCAATCCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

90

Amino Acids

9.98

Weight (kDa)

7.06

Isoelectric Point (pI)

80.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35658 AT2G35658 AT2G35658 AT4G16840
fragaria_vesca FvH4_5g20070 FvH4_5g20070
malus_domestica MD04G1050500.v1.1 MD06G1049300.v1.1
prunus_persica Prupe.5G059800_v2.0.a1
pyrus_communis pycom04g04440
rosa_chinensis RchiOBHm_Chr7g0204931
rosa_multiflora Rmu_sc0004124.1_g000005
rosa_roxburghii Rroxscaffold_3G00252660
rosa_rugosa Rorug07G0086600 Rorug07G0086600
rosa_samantha Rh7AG217300 Rh7BG214200 Rh7CG230600 Rh7DG224900
rosa_wichuraiana Rw7G018850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AccII CGCG 1 cut(s) 14
AciI CCGC 4 cut(s) 12, 68, 162, 192
AclWI GGATC 2 cut(s) 3, 140
AgsI TTSAA 1 cut(s) 90
Alw21I GWGCWC 2 cut(s) 22, 117
Alw26I GTCTC 2 cut(s) 34, 183
AlwI GGATC 2 cut(s) 3, 140
Ama87I CYCGRG 1 cut(s) 24
ApeKI GCWGC 1 cut(s) 70
AsuC2I CCSGG 2 cut(s) 34, 44
AsuHPI GGTGA 1 cut(s) 57
AvaI CYCGRG 1 cut(s) 24
Bbv12I GWGCWC 2 cut(s) 22, 117
BbvI GCAGC 1 cut(s) 82
BccI CCATC 1 cut(s) 212
BcnI CCSGG 2 cut(s) 34, 44
BcoDI GTCTC 2 cut(s) 34, 183
BglII AGATCT 1 cut(s) 130
BisI GCNGC 2 cut(s) 71, 162
BlsI GCNGC 2 cut(s) 72, 163
Bme1390I CCNGG 2 cut(s) 34, 44
BmeT110I CYCGRG 1 cut(s) 24
BmrFI CCNGG 2 cut(s) 34, 44
BmsI GCATC 1 cut(s) 192
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpuMI CCSGG 2 cut(s) 34, 44
BsaBI GATNNNNATC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 33
Bse8I GATNNNNATC 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 2 cut(s) 204, 243
BseJI GATNNNNATC 1 cut(s) 129
BseMII CTCAG 1 cut(s) 123
BseRI GAGGAG 1 cut(s) 12
BseXI GCAGC 1 cut(s) 82
Bsh1236I CGCG 1 cut(s) 14
BsiHKAI GWGCWC 2 cut(s) 22, 117
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 2 cut(s) 33, 44
BslFI GGGAC 1 cut(s) 50
BsmAI GTCTC 2 cut(s) 34, 183
BsmFI GGGAC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 24
Bsp1286I GDGCHC 2 cut(s) 22, 117
Bsp143I GATC 4 cut(s) 8, 124, 130, 145
BspACI CCGC 4 cut(s) 12, 68, 162, 192
BspCNI CTCAG 1 cut(s) 122
BspFNI CGCG 1 cut(s) 14
BspPI GGATC 2 cut(s) 3, 140
BspTNI GGTCTC 1 cut(s) 183
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 4 cut(s) 8, 124, 130, 145
Bst4CI ACNGT 1 cut(s) 177
BstDEI CTNAG 1 cut(s) 109
BstF5I GGATG 2 cut(s) 204, 243
BstFNI CGCG 1 cut(s) 14
BstKTI GATC 4 cut(s) 11, 127, 133, 148
BstMAI GTCTC 2 cut(s) 34, 183
BstMBI GATC 4 cut(s) 8, 124, 130, 145
BstMWI GCNNNNNNNGC 3 cut(s) 79, 200, 236
BstSCI CCNGG 2 cut(s) 32, 42
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 82
BstX2I RGATCY 2 cut(s) 8, 130
BstYI RGATCY 2 cut(s) 8, 130
BtsCI GGATG 2 cut(s) 204, 243
CviAII CATG 2 cut(s) 158, 270
DdeI CTNAG 1 cut(s) 109
DpnI GATC 4 cut(s) 10, 126, 132, 147
DpnII GATC 4 cut(s) 8, 124, 130, 145
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 24
FaeI CATG 2 cut(s) 161, 273
FaiI YATR 3 cut(s) 142, 159, 271
FaqI GGGAC 1 cut(s) 50
FatI CATG 2 cut(s) 157, 269
Fnu4HI GCNGC 2 cut(s) 71, 162
FokI GGATG 2 cut(s) 191, 230
Fsp4HI GCNGC 2 cut(s) 71, 162
GluI GCNGC 2 cut(s) 71, 162
HapII CCGG 2 cut(s) 33, 44
Hin1II CATG 2 cut(s) 161, 273
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 1 cut(s) 27
HpaII CCGG 2 cut(s) 33, 44
HphI GGTGA 1 cut(s) 57
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 1 cut(s) 49
HpyCH4III ACNGT 1 cut(s) 177
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 200, 236
HpyF3I CTNAG 1 cut(s) 109
Hsp92II CATG 2 cut(s) 161, 273
Kzo9I GATC 4 cut(s) 8, 124, 130, 145
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 3 cut(s) 46, 57, 235
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 1 cut(s) 192
MalI GATC 4 cut(s) 10, 126, 132, 147
MboI GATC 4 cut(s) 8, 124, 130, 145
MboII GAAGA 3 cut(s) 78, 125, 247
MflI RGATCY 2 cut(s) 8, 130
MhlI GDGCHC 2 cut(s) 22, 117
MlyI GAGTC 1 cut(s) 36
MnlI CCTC 5 cut(s) 33, 161, 228, 236, 257
MseI TTAA 1 cut(s) 212
MspI CCGG 2 cut(s) 33, 44
MspR9I CCNGG 2 cut(s) 34, 44
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 3 cut(s) 79, 200, 236
NciI CCSGG 2 cut(s) 34, 44
NdeII GATC 4 cut(s) 8, 124, 130, 145
NlaIII CATG 2 cut(s) 161, 273
PaeR7I CTCGAG 1 cut(s) 24
PkrI GCNGC 2 cut(s) 72, 163
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PspXI VCTCGAGB 1 cut(s) 24
PsuI RGATCY 2 cut(s) 8, 130
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 2 cut(s) 71, 162
Sau3AI GATC 4 cut(s) 8, 124, 130, 145
SchI GAGTC 1 cut(s) 36
ScrFI CCNGG 2 cut(s) 34, 44
SduI GDGCHC 2 cut(s) 22, 117
SetI ASST 1 cut(s) 220
SfaNI GCATC 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 24
SlaI CTCGAG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 24
SmoI CTYRAG 1 cut(s) 24
SsiI CCGC 4 cut(s) 12, 68, 162, 192
StyD4I CCNGG 2 cut(s) 32, 42
TaaI ACNGT 1 cut(s) 177
TaqI TCGA 1 cut(s) 25
TauI GCSGC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TseI GCWGC 1 cut(s) 70
XhoI CTCGAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.