RchiOBHm_Chr6g0272151

Putative rRNA methylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008049
Physical Location & Seq
Reverse (-)
32224438 .. 32230565
6128 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 810 bp
ATGTTGGCAGTTGTGCCACGGAGGTTATCATGGGGGCTCCCTCATTTGCTGAGCACACAGAAGCAGAAACCTCCAACGTTCCGAAACTTTTCGGGTAATTCTGCCGGAAACGGCTGGTTGGGCAAGTTTGAACTGAACGCTGCAACTTCCAACGACTTGGTTTCGGTTTCTGTGCCCAAACACTCTCCTCTCTCTGGATTGGAGGATCTACTTTTGAGCTTTATCTCGGGCAAGAAGAAGGCCACAGAAGTTGCTCACTCGGTTTGGAAACGTGTGGTCCAAAAAGGAGATACGGTAATTGACGCCACTTGTGGCAATGGCCATGATACCTTAGCAATGCTCAAGATGGTAGCTGATGAATCAGGTAAAGGTTCAGTTTATGGAATGGATATTCAGGAAGTTGCTTTACAGAAAACTTCTTCTTTGCTGGAAGAGTCGGTCAGTCCAAAAGAGAAAGAACTTGTTAAGCTCTTCTCCAAATGTCACAGTAAAATGCGTGAAGTTCTTCTGCAGGATACATGTGTTAGGCTTGTTGCTTTCAACCTAGGCTATCTTCCTGGTGGCGACAAAACAATAATCACTGAATCAGGAACAACACTGAAAGCACTGGAAGTTGCAAAAGATATCCTGCTGCCAGGAGGGCTTATCAGCTTAGTGGTTTATGTGGGGCATCCTGGTGGATGGGAAGAACTGCAGATGGTACAACACTTTGTTTCTAAACTATCCGTTGAGAAATGGATCTGCTGTGAGTATCAGTCATCAAACCGATCATGGTCTCCAGTACTTGTTTTCTTATTCAAAAGATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
rRNA_methylase

Protein Analysis

269

Amino Acids

29.5

Weight (kDa)

8.81

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 95 - 142 1.6e-06 Methyltransferase domain
rRNA_methylase PF06962 126 - 266 1.3e-41 Putative rRNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 77
AclWI GGATC 2 cut(s) 213, 746
AcoI YGGCCR 1 cut(s) 319
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 2 cut(s) 702, 783
AfiI CCNNNNNNNGG 1 cut(s) 194
AflIII ACRYGT 2 cut(s) 271, 518
AgsI TTSAA 3 cut(s) 131, 541, 799
AjnI CCWGG 3 cut(s) 556, 634, 673
AluBI AGCT 4 cut(s) 219, 353, 469, 651
AluI AGCT 4 cut(s) 219, 353, 469, 651
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 1 cut(s) 780
AlwI GGATC 2 cut(s) 213, 746
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 2 cut(s) 240, 319
ApeKI GCWGC 2 cut(s) 140, 631
Asp700I GAANNNNTTC 2 cut(s) 88, 504
AspA2I CCTAGG 1 cut(s) 544
AspS9I GGNCC 1 cut(s) 277
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 1 cut(s) 277
AvrII CCTAGG 1 cut(s) 544
BaeGI GKGCMC 1 cut(s) 177
BaeI ACNNNNGTAYC 2 cut(s) 507, 540
BalI TGGCCA 1 cut(s) 321
BanII GRGCYC 1 cut(s) 39
BarI GAAGNNNNNNTAC 2 cut(s) 390, 422
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 127, 618
BccI CCATC 3 cut(s) 340, 675, 691
BceAI ACGGC 1 cut(s) 127
BciT130I CCWGG 3 cut(s) 558, 636, 675
BciVI GTATCC 1 cut(s) 508
BcoDI GTCTC 1 cut(s) 780
BfaI CTAG 1 cut(s) 545
BfmI CTRYAG 2 cut(s) 509, 692
BfuI GTATCC 1 cut(s) 508
BglI GCCNNNNNGGC 1 cut(s) 640
BisI GCNGC 2 cut(s) 141, 632
BlnI CCTAGG 1 cut(s) 544
BlpI GCTNAGC 1 cut(s) 50
BlsI GCNGC 2 cut(s) 142, 633
BmcAI AGTACT 1 cut(s) 783
Bme1390I CCNGG 3 cut(s) 558, 636, 675
Bme18I GGWCC 1 cut(s) 277
BmeT110I CYCGRG 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 1 cut(s) 38
BmrFI CCNGG 3 cut(s) 558, 636, 675
BmsI GCATC 2 cut(s) 679, 794
BpmI CTGGAG 1 cut(s) 762
Bpu10I CCTNAGC 1 cut(s) 331
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 780
BsaJI CCNNGG 2 cut(s) 17, 544
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse1I ACTGG 2 cut(s) 612, 779
Bse3DI GCAATG 2 cut(s) 322, 342
BseBI CCWGG 3 cut(s) 558, 636, 675
BseDI CCNNGG 2 cut(s) 17, 544
BseGI GGATG 2 cut(s) 670, 686
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 2 cut(s) 322, 342
BseMII CTCAG 1 cut(s) 41
BseNI ACTGG 2 cut(s) 612, 779
BseRI GAGGAG 1 cut(s) 177
BseSI GKGCMC 1 cut(s) 177
BseXI GCAGC 2 cut(s) 127, 618
BshFI GGCC 2 cut(s) 242, 321
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 226
BsiSI CCGG 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 780
BsnI GGCC 2 cut(s) 242, 321
Bso31I GGTCTC 1 cut(s) 780
BsoBI CYCGRG 1 cut(s) 226
Bsp1286I GDGCHC 3 cut(s) 39, 56, 177
Bsp143I GATC 3 cut(s) 205, 738, 767
Bsp1720I GCTNAGC 1 cut(s) 50
BspANI GGCC 2 cut(s) 242, 321
BspCNI CTCAG 1 cut(s) 42
BspLI GGNNCC 1 cut(s) 38
BspMAI CTGCAG 2 cut(s) 513, 696
BspPI GGATC 2 cut(s) 213, 746
BspQI GCTCTTC 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 780
BsrDI GCAATG 2 cut(s) 322, 342
BsrI ACTGG 2 cut(s) 612, 779
BssECI CCNNGG 2 cut(s) 17, 544
BssMI GATC 3 cut(s) 205, 738, 767
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 544
Bst2UI CCWGG 3 cut(s) 558, 636, 675
Bst4CI ACNGT 2 cut(s) 295, 488
Bst6I CTCTTC 2 cut(s) 426, 476
BstACI GRCGYC 1 cut(s) 303
BstDEI CTNAG 3 cut(s) 50, 331, 652
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 2 cut(s) 670, 686
BstKTI GATC 3 cut(s) 208, 741, 770
BstMAI GTCTC 1 cut(s) 780
BstMBI GATC 3 cut(s) 205, 738, 767
BstMWI GCNNNNNNNGC 2 cut(s) 120, 640
BstNI CCWGG 3 cut(s) 558, 636, 675
BstNSI RCATGY 1 cut(s) 522
BstSCI CCNGG 3 cut(s) 556, 634, 673
BstSFI CTRYAG 2 cut(s) 509, 692
BstSLI GKGCMC 1 cut(s) 177
BstV1I GCAGC 2 cut(s) 127, 618
BstX2I RGATCY 2 cut(s) 205, 738
BstXI CCANNNNNNTGG 1 cut(s) 157
BstYI RGATCY 2 cut(s) 205, 738
BsuI GTATCC 1 cut(s) 508
BsuRI GGCC 2 cut(s) 242, 321
BtgI CCRYGG 1 cut(s) 17
BtsCI GGATG 2 cut(s) 670, 686
BtsIMutI CAGTG 3 cut(s) 579, 596, 605
Cfr13I GGNCC 1 cut(s) 277
CseI GACGC 1 cut(s) 311
Csp6I GTAC 2 cut(s) 701, 782
CspCI CAANNNNNGTGG 2 cut(s) 232, 267
CviAII CATG 4 cut(s) 30, 323, 519, 771
CviQI GTAC 2 cut(s) 701, 782
DdeI CTNAG 3 cut(s) 50, 331, 652
DpnI GATC 3 cut(s) 207, 740, 769
DpnII GATC 3 cut(s) 205, 738, 767
EaeI YGGCCR 1 cut(s) 319
Eam1104I CTCTTC 2 cut(s) 426, 476
EarI CTCTTC 2 cut(s) 426, 476
Eco130I CCWWGG 1 cut(s) 544
Eco24I GRGCYC 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 780
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 226
EcoRII CCWGG 3 cut(s) 556, 634, 673
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 544
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 544
FaeI CATG 4 cut(s) 33, 326, 522, 774
FaiI YATR 6 cut(s) 31, 324, 381, 520, 663, 772
FatI CATG 4 cut(s) 29, 322, 518, 770
Fnu4HI GCNGC 2 cut(s) 141, 632
FokI GGATG 2 cut(s) 657, 693
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 2 cut(s) 141, 632
FspBI CTAG 1 cut(s) 545
GluI GCNGC 2 cut(s) 141, 632
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 2 cut(s) 242, 321
HapII CCGG 1 cut(s) 105
HgaI GACGC 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 4 cut(s) 33, 326, 522, 774
HinfI GANTC 3 cut(s) 359, 434, 584
HpaII CCGG 1 cut(s) 105
Hpy188I TCNGA 1 cut(s) 83
Hpy188III TCNNGA 4 cut(s) 195, 343, 395, 588
HpyAV CCTTC 1 cut(s) 232
HpyCH4III ACNGT 2 cut(s) 295, 488
HpyCH4IV ACGT 2 cut(s) 77, 271
HpyCH4V TGCA 4 cut(s) 143, 511, 617, 694
HpyF10VI GCNNNNNNNGC 2 cut(s) 120, 640
HpyF3I CTNAG 3 cut(s) 50, 331, 652
HpySE526I ACGT 2 cut(s) 77, 271
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 4 cut(s) 33, 326, 522, 774
Kzo9I GATC 3 cut(s) 205, 738, 767
LguI GCTCTTC 1 cut(s) 476
LmnI GCTCC 1 cut(s) 42
Lsp1109I GCAGC 2 cut(s) 127, 618
LweI GCATC 2 cut(s) 679, 794
MaeI CTAG 1 cut(s) 545
MaeII ACGT 2 cut(s) 77, 271
MaeIII GTNAC 1 cut(s) 482
MalI GATC 3 cut(s) 207, 740, 769
MboI GATC 3 cut(s) 205, 738, 767
MboII GAAGA 7 cut(s) 247, 411, 443, 463, 497, 545, 698
MflI RGATCY 2 cut(s) 205, 738
MhlI GDGCHC 3 cut(s) 39, 56, 177
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 2 cut(s) 97, 297
MluNI TGGCCA 1 cut(s) 321
MlyI GAGTC 1 cut(s) 443
MmeI TCCRAC 2 cut(s) 98, 174
MnlI CCTC 6 cut(s) 15, 51, 81, 196, 198, 632
Mox20I TGGCCA 1 cut(s) 321
MroXI GAANNNNTTC 2 cut(s) 88, 504
MscI TGGCCA 1 cut(s) 321
MseI TTAA 1 cut(s) 465
MslI CAYNNNNRTG 1 cut(s) 675
Msp20I TGGCCA 1 cut(s) 321
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 3 cut(s) 558, 636, 675
MvaI CCWGG 3 cut(s) 558, 636, 675
MwoI GCNNNNNNNGC 2 cut(s) 120, 640
NdeII GATC 3 cut(s) 205, 738, 767
NlaIII CATG 4 cut(s) 33, 326, 522, 774
NlaIV GGNNCC 1 cut(s) 38
NmuCI GTSAC 1 cut(s) 482
NspI RCATGY 1 cut(s) 522
PciI ACATGT 1 cut(s) 518
PciSI GCTCTTC 1 cut(s) 476
PdmI GAANNNNTTC 2 cut(s) 88, 504
PfeI GAWTC 2 cut(s) 359, 584
PkrI GCNGC 2 cut(s) 142, 633
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
PscI ACATGT 1 cut(s) 518
Psp1406I AACGTT 1 cut(s) 77
Psp6I CCWGG 3 cut(s) 556, 634, 673
PspGI CCWGG 3 cut(s) 556, 634, 673
PspN4I GGNNCC 1 cut(s) 38
PspPI GGNCC 1 cut(s) 277
PstI CTGCAG 2 cut(s) 513, 696
PsuI RGATCY 2 cut(s) 205, 738
RsaI GTAC 2 cut(s) 702, 783
RsaNI GTAC 2 cut(s) 701, 782
RseI CAYNNNNRTG 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 476
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 2 cut(s) 141, 632
Sau3AI GATC 3 cut(s) 205, 738, 767
Sau96I GGNCC 1 cut(s) 277
ScaI AGTACT 1 cut(s) 783
SchI GAGTC 1 cut(s) 443
ScrFI CCNGG 3 cut(s) 558, 636, 675
SduI GDGCHC 3 cut(s) 39, 56, 177
SfaNI GCATC 2 cut(s) 679, 794
SfcI CTRYAG 2 cut(s) 509, 692
SinI GGWCC 1 cut(s) 277
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 2 cut(s) 97, 297
SspMI CTAG 1 cut(s) 545
StyD4I CCNGG 3 cut(s) 556, 634, 673
StyI CCWWGG 1 cut(s) 544
TaaI ACNGT 2 cut(s) 295, 488
TaiI ACGT 2 cut(s) 80, 274
TaqII GACCGA 1 cut(s) 427
TasI AATT 2 cut(s) 97, 297
TatI WGTACW 1 cut(s) 781
TfiI GAWTC 2 cut(s) 359, 584
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 3 cut(s) 586, 603, 612
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 2 cut(s) 140, 631
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 1 cut(s) 372
TspGWI ACGGA 2 cut(s) 34, 715
TspRI CASTG 3 cut(s) 586, 603, 612
VpaK11BI GGWCC 1 cut(s) 277
XceI RCATGY 1 cut(s) 522
XmaJI CCTAGG 1 cut(s) 544
XmnI GAANNNNTTC 2 cut(s) 88, 504
XspI CTAG 1 cut(s) 545
ZrmI AGTACT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.