RchiOBHm_Chr6g0274681

5-formyltetrahydrofolate cyclo-ligase-like protein COG0212

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009639
Physical Location & Seq
Reverse (-)
36195811 .. 36200077
4267 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 501 bp
ATGATGACTCCTAGTACTTTGAAAGAGGCTTGCACCTCTGTTGGGGTGGCCAAACACGGGCGGCCTATTGGATTAGATGAGAAAATCAAGGTTGATCTTATTGTCATTGGCTCCGTTGCTGTTGATCCCAGGACAGGTGCTCGACTTGGCAAGGGTGAGGGATTTGCAGAGCTTGAATATGGGATGCTGCGATACATGGGAGCCATTGATGATTCAACACCAGTTGTCACTACTGTGCATGACAGTCAATTAGTAGATGATATTCCAATTGAGAAGCTGTTAGTCCATGATGTCATATTTACCAACACATCAATTCCAAAGCCTCAAGGGATTTACTGGGACAAATTGTCTCCGGAGAAGCTGGGGCAAATTAAAATACTCAGAGAGCTGAAGAGAAGGATTGAACAAGAGACTGGACAAAAATTGCCATGCGGTCCATCAGAGAAACTGCCTCCCACAGCGCGAAGAGGAGCTAACTTGGTAGGTGGTAAAAGGATATAA

Protein Analysis

166

Amino Acids

18.12

Weight (kDa)

8.87

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 25 - 98 4e-16 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 463
AccIII TCCGGA 1 cut(s) 352
AciI CCGC 2 cut(s) 61, 432
AclWI GGATC 1 cut(s) 119
AcoI YGGCCR 1 cut(s) 48
AcuI CTGAAG 1 cut(s) 410
AfaI GTAC 1 cut(s) 16
AfiI CCNNNNNNNGG 3 cut(s) 42, 57, 134
AgsI TTSAA 4 cut(s) 22, 176, 216, 404
AhdI GACNNNNNGTC 1 cut(s) 346
AjnI CCWGG 1 cut(s) 128
AleI CACNNNNGTG 1 cut(s) 233
AluBI AGCT 5 cut(s) 172, 277, 361, 388, 473
AluI AGCT 5 cut(s) 172, 277, 361, 388, 473
Alw21I GWGCWC 1 cut(s) 142
Alw26I GTCTC 2 cut(s) 354, 404
AlwI GGATC 1 cut(s) 119
Aor13HI TCCGGA 1 cut(s) 352
AoxI GGCC 2 cut(s) 48, 62
ApeKI GCWGC 1 cut(s) 187
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 1 cut(s) 434
AsuHPI GGTGA 1 cut(s) 167
AvaII GGWCC 1 cut(s) 434
BalI TGGCCA 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 142
BbvI GCAGC 1 cut(s) 174
BccI CCATC 1 cut(s) 445
BciT130I CCWGG 1 cut(s) 130
BcoDI GTCTC 2 cut(s) 354, 404
BfaI CTAG 1 cut(s) 12
BisI GCNGC 2 cut(s) 62, 188
BlsI GCNGC 2 cut(s) 63, 189
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 1 cut(s) 130
Bme18I GGWCC 1 cut(s) 434
BmeRI GACNNNNNGTC 1 cut(s) 346
BmgT120I GGNCC 1 cut(s) 434
BmiI GGNNCC 2 cut(s) 112, 202
BmrFI CCNGG 1 cut(s) 130
BmrI ACTGGG 1 cut(s) 346
BmsI GCATC 1 cut(s) 174
BmuI ACTGGG 1 cut(s) 346
BpuEI CTTGAG 1 cut(s) 309
BsaJI CCNNGG 1 cut(s) 128
BsaWI WCCGGW 1 cut(s) 352
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 57, 134
Bse1I ACTGG 3 cut(s) 221, 341, 418
BseAI TCCGGA 1 cut(s) 352
BseBI CCWGG 1 cut(s) 130
BseDI CCNNGG 1 cut(s) 128
BseGI GGATG 1 cut(s) 189
BseLI CCNNNNNNNGG 3 cut(s) 42, 57, 134
BseMII CTCAG 1 cut(s) 394
BseNI ACTGG 3 cut(s) 221, 341, 418
BseRI GAGGAG 1 cut(s) 483
BseXI GCAGC 1 cut(s) 174
BseYI CCCAGC 1 cut(s) 361
Bsh1236I CGCG 1 cut(s) 463
BshFI GGCC 2 cut(s) 50, 64
BsiHKAI GWGCWC 1 cut(s) 142
BsiSI CCGG 1 cut(s) 353
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 3 cut(s) 42, 57, 134
BsmAI GTCTC 2 cut(s) 354, 404
BsmFI GGGAC 1 cut(s) 353
BsnI GGCC 2 cut(s) 50, 64
Bsp1286I GDGCHC 1 cut(s) 142
Bsp13I TCCGGA 1 cut(s) 352
Bsp143I GATC 2 cut(s) 94, 124
BspACI CCGC 2 cut(s) 61, 432
BspANI GGCC 2 cut(s) 50, 64
BspCNI CTCAG 1 cut(s) 393
BspEI TCCGGA 1 cut(s) 352
BspFNI CGCG 1 cut(s) 463
BspLI GGNNCC 2 cut(s) 112, 202
BspPI GGATC 1 cut(s) 119
BsrI ACTGG 3 cut(s) 221, 341, 418
BssECI CCNNGG 1 cut(s) 128
BssMI GATC 2 cut(s) 94, 124
Bst2UI CCWGG 1 cut(s) 130
Bst4CI ACNGT 2 cut(s) 235, 245
Bst6I CTCTTC 2 cut(s) 386, 460
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 1 cut(s) 380
BstF5I GGATG 1 cut(s) 189
BstFNI CGCG 1 cut(s) 463
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 2 cut(s) 97, 127
BstMAI GTCTC 2 cut(s) 354, 404
BstMBI GATC 2 cut(s) 94, 124
BstNI CCWGG 1 cut(s) 130
BstSCI CCNGG 1 cut(s) 128
BstUI CGCG 1 cut(s) 463
BstV1I GCAGC 1 cut(s) 174
BsuRI GGCC 2 cut(s) 50, 64
BtsCI GGATG 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 31
CfoI GCGC 1 cut(s) 463
Cfr13I GGNCC 1 cut(s) 434
Csp6I GTAC 1 cut(s) 15
CviAII CATG 4 cut(s) 196, 239, 287, 429
CviQI GTAC 1 cut(s) 15
DdeI CTNAG 1 cut(s) 380
DpnI GATC 2 cut(s) 96, 126
DpnII GATC 2 cut(s) 94, 124
DriI GACNNNNNGTC 1 cut(s) 346
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 2 cut(s) 386, 460
Eam1105I GACNNNNNGTC 1 cut(s) 346
EarI CTCTTC 2 cut(s) 386, 460
Eco47I GGWCC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 128
FaeI CATG 4 cut(s) 199, 242, 290, 432
FaiI YATR 7 cut(s) 180, 197, 240, 288, 296, 430, 499
FaqI GGGAC 1 cut(s) 353
FatI CATG 4 cut(s) 195, 238, 286, 428
Fnu4HI GCNGC 2 cut(s) 62, 188
FokI GGATG 1 cut(s) 196
Fsp4HI GCNGC 2 cut(s) 62, 188
FspBI CTAG 1 cut(s) 12
GlaI GCGC 1 cut(s) 462
GluI GCNGC 2 cut(s) 62, 188
GsaI CCCAGC 1 cut(s) 365
HaeIII GGCC 2 cut(s) 50, 64
HapII CCGG 1 cut(s) 353
HhaI GCGC 1 cut(s) 463
Hin1II CATG 4 cut(s) 199, 242, 290, 432
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HinfI GANTC 2 cut(s) 7, 212
HpaII CCGG 1 cut(s) 353
HphI GGTGA 1 cut(s) 167
Hpy188I TCNGA 2 cut(s) 383, 442
Hpy188III TCNNGA 1 cut(s) 353
HpyAV CCTTC 1 cut(s) 390
HpyCH4III ACNGT 2 cut(s) 235, 245
HpyCH4V TGCA 3 cut(s) 33, 167, 238
HpyF3I CTNAG 1 cut(s) 380
Hsp92II CATG 4 cut(s) 199, 242, 290, 432
HspAI GCGC 1 cut(s) 461
Kpn2I TCCGGA 1 cut(s) 352
Kzo9I GATC 2 cut(s) 94, 124
LmnI GCTCC 3 cut(s) 116, 200, 470
LpnPI CCDG 8 cut(s) 115, 120, 142, 234, 322, 347, 366, 399
Lsp1109I GCAGC 1 cut(s) 174
LweI GCATC 1 cut(s) 174
MaeI CTAG 1 cut(s) 12
MaeIII GTNAC 1 cut(s) 226
MalI GATC 2 cut(s) 96, 126
MboI GATC 2 cut(s) 94, 124
MboII GAAGA 2 cut(s) 403, 477
MfeI CAATTG 1 cut(s) 267
MhlI GDGCHC 1 cut(s) 142
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 6 cut(s) 248, 267, 312, 344, 369, 422
MluNI TGGCCA 1 cut(s) 50
MnlI CCTC 6 cut(s) 19, 46, 151, 333, 461, 462
Mox20I TGGCCA 1 cut(s) 50
MroI TCCGGA 1 cut(s) 352
MscI TGGCCA 1 cut(s) 50
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 1 cut(s) 233
Msp20I TGGCCA 1 cut(s) 50
MspI CCGG 1 cut(s) 353
MspR9I CCNGG 1 cut(s) 130
MunI CAATTG 1 cut(s) 267
MvaI CCWGG 1 cut(s) 130
MvnI CGCG 1 cut(s) 463
NdeII GATC 2 cut(s) 94, 124
NlaIII CATG 4 cut(s) 199, 242, 290, 432
NlaIV GGNNCC 2 cut(s) 112, 202
NmuCI GTSAC 1 cut(s) 226
OliI CACNNNNGTG 1 cut(s) 233
PfeI GAWTC 1 cut(s) 212
PkrI GCNGC 2 cut(s) 63, 189
Psp6I CCWGG 1 cut(s) 128
PspFI CCCAGC 1 cut(s) 361
PspGI CCWGG 1 cut(s) 128
PspN4I GGNNCC 2 cut(s) 112, 202
PspPI GGNCC 1 cut(s) 434
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
RseI CAYNNNNRTG 1 cut(s) 233
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 2 cut(s) 62, 188
Sau3AI GATC 2 cut(s) 94, 124
Sau96I GGNCC 1 cut(s) 434
ScaI AGTACT 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 130
SduI GDGCHC 1 cut(s) 142
SetI ASST 9 cut(s) 38, 93, 139, 174, 279, 363, 390, 475, 487
SfaNI GCATC 1 cut(s) 174
SinI GGWCC 1 cut(s) 434
SmiMI CAYNNNNRTG 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 324
SmoI CTYRAG 1 cut(s) 324
Sse9I AATT 6 cut(s) 248, 267, 312, 344, 369, 422
SsiI CCGC 2 cut(s) 61, 432
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 1 cut(s) 128
TaaI ACNGT 2 cut(s) 235, 245
TaqI TCGA 1 cut(s) 142
TasI AATT 6 cut(s) 248, 267, 312, 344, 369, 422
TatI WGTACW 1 cut(s) 14
TauI GCSGC 1 cut(s) 64
TfiI GAWTC 1 cut(s) 212
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 1 cut(s) 187
Tsp45I GTSAC 1 cut(s) 226
TspGWI ACGGA 1 cut(s) 103
VpaK11BI GGWCC 1 cut(s) 434
XspI CTAG 1 cut(s) 12
ZrmI AGTACT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.