RchiOBHm_Chr6g0291501

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0016518
Physical Location & Seq
Reverse (-)
54623185 .. 54624586
1402 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1083 bp
ATGGAATTTAAGCACAGAAAGCTGAGTAGCCCAACCTGCGATGATTGCATGAAGCGTGATCCTAATAAGAACTGTCCATCAATATGTAACCATAAACCCAAAATCTTCATAGTCGTCGTATCGTCGTTGGCTGCAATCGCTTTGCTTGTTATTTGCGGCTATTTTCTCTATGTAAAGTACTACAAAAACAGAAGAAGATCACCATCACAACCACAACCACAACAACATCTACCTCATCAGGAGGAGGAGACTCTTCATCATCATGATCATCATGCTGAGTTGTTTATTGATGAAGAGCATGGACCTGTGGTTGACCACCCCATATGGTACATCCGCACCGTCGGTCTGCAACAATCGCTTATCAGCTCCATCAGCGTGTGCCAGTACAAGAAAGGTGATGGCCTTATCGAAGGGACAGAGTGCGCTGTGTGCTTGAGTGAGTTCCAAGAAGATGAGACTTTGAGACTTTTGCCAAAGTGTGATCACGCTTTTCATATTCCCTGTATTGATACTTGGCTCACATCTCATACAAATTGCCCCTTGTGCCGCGCCCCCATTATCAAAACCGGCGCAACAGATGTGGCACCACCCGAGCCATTTGTTGCAGCTGATTCAAGTGAGCCAGCAGAGGAAGAATCACCGGTCGAGGTTCTAGACAATACAGGTGAGTCCAGTACTGGAGGAGTTGAAGATGAAATTTGTGATGGGATTATTAGGACTACTGAAGCAGGGGAAGATGATATCATTAACAAACAGATCTGTATTAATGAGGAGGAGGTGGATGATGATGTGGAGGAGCCAAGAAGAAGATCGGTTTCCATGGATTCTGCATCAGCTTTGAAGATTAGTCTTGCGGTTTCTAATGTTGTTGAGGTGGATAAATCAAACCGGGGGATTGAAATTGTTGCCAAGAGAGTTGGGGGAGATCAGAAATTGTCAAGGCAGAAAGGTAGTTCTTCAAGAGGGGCATCTTTACAAAATGGAAAACAAACAATTCAGAAGTCGTCTTCCTGGAATGGCAAGTTCTCATTATGCAGGGACATCAACAACTGTGACTCTGTTGTTCCACTGCGAAGCTTTTGA

Protein Analysis

360

Amino Acids

40.02

Weight (kDa)

5.34

Isoelectric Point (pI)

65.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 140 - 183 2.4e-13 Ring finger domain
zf-RING_11 PF17123 140 - 169 4.4e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 140 - 182 1.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 141 - 183 2.2e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 141 - 182 5.3e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016518)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33565
fragaria_vesca FvH4_2g23890
prunus_persica Prupe.8G066400_v2.0.a1 Prupe.8G066400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0291501
rosa_laevigata RLG00000012111
rosa_multiflora Rmu_sc0024207.1_g000003 Rmu_sc0026626.1_g000002
rosa_rugosa Rorug06G0220000
rosa_samantha Rh6AG332100 Rh6BG339000 Rh6CG345200 Rh6DG332600
rosa_wichuraiana Rw6G028850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 583
AccII CGCG 1 cut(s) 549
AciI CCGC 4 cut(s) 156, 334, 547, 854
AclWI GGATC 1 cut(s) 53
AcsI RAATTY 2 cut(s) 5, 696
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 4 cut(s) 179, 329, 386, 676
AgeI ACCGGT 1 cut(s) 640
AgsI TTSAA 5 cut(s) 615, 689, 841, 899, 960
AjnI CCWGG 1 cut(s) 1010
AluBI AGCT 5 cut(s) 22, 366, 608, 836, 1077
AluI AGCT 5 cut(s) 22, 366, 608, 836, 1077
Alw26I GTCTC 3 cut(s) 242, 449, 457
AlwI GGATC 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 590
AoxI GGCC 1 cut(s) 400
ApeKI GCWGC 2 cut(s) 131, 605
ApoI RAATTY 2 cut(s) 5, 696
AseI ATTAAT 1 cut(s) 765
AsiGI ACCGGT 1 cut(s) 640
AspLEI GCGC 3 cut(s) 425, 551, 572
AspS9I GGNCC 1 cut(s) 302
AsuC2I CCSGG 1 cut(s) 890
AsuHPI GGTGA 4 cut(s) 192, 407, 630, 677
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 1 cut(s) 302
BanI GGYRCC 1 cut(s) 583
BbsI GAAGAC 1 cut(s) 999
BbvI GCAGC 2 cut(s) 118, 617
BccI CCATC 5 cut(s) 85, 211, 377, 392, 698
BciT130I CCWGG 1 cut(s) 1012
BclI TGATCA 2 cut(s) 265, 481
BcnI CCSGG 1 cut(s) 890
BcoDI GTCTC 3 cut(s) 242, 449, 457
BfaI CTAG 1 cut(s) 653
BfuAI ACCTGC 1 cut(s) 44
BglII AGATCT 1 cut(s) 756
BisI GCNGC 4 cut(s) 132, 157, 547, 606
BlsI GCNGC 4 cut(s) 133, 158, 548, 607
BmcAI AGTACT 2 cut(s) 179, 676
Bme1390I CCNGG 2 cut(s) 890, 1012
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 585, 798
BmrFI CCNGG 2 cut(s) 890, 1012
BmsI GCATC 2 cut(s) 839, 977
BpiI GAAGAC 1 cut(s) 999
BpmI CTGGAG 1 cut(s) 699
BpuEI CTTGAG 1 cut(s) 454
BpuMI CCSGG 1 cut(s) 890
BsaBI GATNNNNATC 1 cut(s) 202
BsaJI CCNNGG 2 cut(s) 819, 889
BsaWI WCCGGW 1 cut(s) 640
Bse118I RCCGGY 2 cut(s) 566, 640
Bse1I ACTGG 3 cut(s) 382, 672, 682
Bse8I GATNNNNATC 1 cut(s) 202
BseBI CCWGG 1 cut(s) 1012
BseDI CCNNGG 2 cut(s) 819, 889
BseGI GGATG 2 cut(s) 330, 787
BseJI GATNNNNATC 1 cut(s) 202
BseMII CTCAG 2 cut(s) 14, 267
BseNI ACTGG 3 cut(s) 382, 672, 682
BseRI GAGGAG 6 cut(s) 257, 260, 696, 785, 788, 809
BseXI GCAGC 2 cut(s) 118, 617
Bsh1236I CGCG 1 cut(s) 549
Bsh1285I CGRYCG 1 cut(s) 645
BshFI GGCC 1 cut(s) 402
BshNI GGYRCC 1 cut(s) 583
BshTI ACCGGT 1 cut(s) 640
BsiEI CGRYCG 1 cut(s) 645
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 3 cut(s) 567, 641, 889
BslFI GGGAC 2 cut(s) 427, 1052
BsmAI GTCTC 3 cut(s) 242, 449, 457
BsmFI GGGAC 2 cut(s) 427, 1052
BsnI GGCC 1 cut(s) 402
BsoBI CYCGRG 1 cut(s) 590
Bsp143I GATC 7 cut(s) 58, 197, 265, 481, 756, 809, 925
Bsp19I CCATGG 1 cut(s) 819
BspACI CCGC 4 cut(s) 156, 334, 547, 854
BspANI GGCC 1 cut(s) 402
BspCNI CTCAG 2 cut(s) 15, 268
BspFNI CGCG 1 cut(s) 549
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 585, 798
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 1 cut(s) 53
BspQI GCTCTTC 1 cut(s) 288
BspT107I GGYRCC 1 cut(s) 583
BsrFI RCCGGY 2 cut(s) 566, 640
BsrI ACTGG 3 cut(s) 382, 672, 682
BssAI RCCGGY 2 cut(s) 566, 640
BssECI CCNNGG 2 cut(s) 819, 889
BssMI GATC 7 cut(s) 58, 197, 265, 481, 756, 809, 925
BssT1I CCWWGG 1 cut(s) 819
Bst2UI CCWGG 1 cut(s) 1012
Bst4CI ACNGT 3 cut(s) 74, 340, 1052
Bst6I CTCTTC 2 cut(s) 258, 288
BstC8I GCNNGC 1 cut(s) 624
BstDEI CTNAG 2 cut(s) 23, 276
BstDSI CCRYGG 1 cut(s) 819
BstF5I GGATG 2 cut(s) 330, 787
BstFNI CGCG 1 cut(s) 549
BstHHI GCGC 3 cut(s) 425, 551, 572
BstKTI GATC 7 cut(s) 61, 200, 268, 484, 759, 812, 928
BstMAI GTCTC 3 cut(s) 242, 449, 457
BstMBI GATC 7 cut(s) 58, 197, 265, 481, 756, 809, 925
BstMCI CGRYCG 1 cut(s) 645
BstMWI GCNNNNNNNGC 8 cut(s) 19, 36, 45, 137, 355, 372, 429, 543
BstNI CCWGG 1 cut(s) 1012
BstSCI CCNGG 2 cut(s) 888, 1010
BstUI CGCG 1 cut(s) 549
BstV1I GCAGC 2 cut(s) 118, 617
BstV2I GAAGAC 1 cut(s) 999
BstX2I RGATCY 1 cut(s) 756
BstYI RGATCY 1 cut(s) 756
BsuRI GGCC 1 cut(s) 402
BtgI CCRYGG 1 cut(s) 819
BtgZI GCGATG 1 cut(s) 54
BtsCI GGATG 2 cut(s) 330, 787
BtsI GCAGTG 1 cut(s) 1067
BtsIMutI CAGTG 1 cut(s) 1067
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 1 cut(s) 624
CciI TCATGA 1 cut(s) 262
CfoI GCGC 3 cut(s) 425, 551, 572
Cfr10I RCCGGY 2 cut(s) 566, 640
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 4 cut(s) 178, 328, 385, 675
CspAI ACCGGT 1 cut(s) 640
CspCI CAANNNNNGTGG 2 cut(s) 561, 596
CviAII CATG 5 cut(s) 49, 263, 272, 299, 820
CviQI GTAC 4 cut(s) 178, 328, 385, 675
DdeI CTNAG 2 cut(s) 23, 276
DpnI GATC 7 cut(s) 60, 199, 267, 483, 758, 811, 927
DpnII GATC 7 cut(s) 58, 197, 265, 481, 756, 809, 925
Eam1104I CTCTTC 2 cut(s) 258, 288
EarI CTCTTC 2 cut(s) 258, 288
Eco130I CCWWGG 1 cut(s) 819
Eco32I GATATC 1 cut(s) 742
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 744
Eco88I CYCGRG 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 1010
EcoRV GATATC 1 cut(s) 742
EcoT14I CCWWGG 1 cut(s) 819
ErhI CCWWGG 1 cut(s) 819
FaeI CATG 5 cut(s) 52, 266, 275, 302, 823
FaqI GGGAC 2 cut(s) 427, 1052
FatI CATG 5 cut(s) 48, 262, 271, 298, 819
FauNDI CATATG 1 cut(s) 323
FbaI TGATCA 2 cut(s) 265, 481
Fnu4HI GCNGC 4 cut(s) 132, 157, 547, 606
FokI GGATG 2 cut(s) 317, 794
Fsp4HI GCNGC 4 cut(s) 132, 157, 547, 606
FspBI CTAG 1 cut(s) 653
GlaI GCGC 3 cut(s) 424, 550, 571
GluI GCNGC 4 cut(s) 132, 157, 547, 606
GsuI CTGGAG 1 cut(s) 699
HaeIII GGCC 1 cut(s) 402
HapII CCGG 3 cut(s) 567, 641, 889
HhaI GCGC 3 cut(s) 425, 551, 572
Hin1II CATG 5 cut(s) 52, 266, 275, 302, 823
Hin6I GCGC 3 cut(s) 423, 549, 570
HinP1I GCGC 3 cut(s) 423, 549, 570
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 1075
HinfI GANTC 6 cut(s) 250, 611, 635, 668, 824, 1055
HpaII CCGG 3 cut(s) 567, 641, 889
HphI GGTGA 4 cut(s) 192, 407, 630, 677
Hpy166II GTNNAC 1 cut(s) 313
Hpy188I TCNGA 2 cut(s) 930, 999
Hpy188III TCNNGA 4 cut(s) 239, 263, 653, 960
Hpy8I GTNNAC 1 cut(s) 313
Hpy99I CGWCG 3 cut(s) 119, 127, 344
HpyAV CCTTC 1 cut(s) 404
HpyCH4III ACNGT 3 cut(s) 74, 340, 1052
HpyCH4V TGCA 6 cut(s) 48, 134, 349, 605, 830, 1035
HpyF10VI GCNNNNNNNGC 8 cut(s) 19, 36, 45, 137, 355, 372, 429, 543
HpyF3I CTNAG 2 cut(s) 23, 276
Hsp92II CATG 5 cut(s) 52, 266, 275, 302, 823
HspAI GCGC 3 cut(s) 423, 549, 570
Ksp22I TGATCA 2 cut(s) 265, 481
Kzo9I GATC 7 cut(s) 58, 197, 265, 481, 756, 809, 925
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 2 cut(s) 371, 796
Lsp1109I GCAGC 2 cut(s) 118, 617
LweI GCATC 2 cut(s) 839, 977
MaeI CTAG 1 cut(s) 653
MaeIII GTNAC 2 cut(s) 86, 1052
MalI GATC 7 cut(s) 60, 199, 267, 483, 758, 811, 927
MboI GATC 7 cut(s) 58, 197, 265, 481, 756, 809, 925
MflI RGATCY 1 cut(s) 756
MluCI AATT 6 cut(s) 5, 532, 696, 900, 932, 993
MlyI GAGTC 3 cut(s) 244, 677, 1049
MseI TTAA 3 cut(s) 9, 747, 765
MslI CAYNNNNRTG 3 cut(s) 82, 261, 374
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 3 cut(s) 567, 641, 889
MspR9I CCNGG 2 cut(s) 890, 1012
MvaI CCWGG 1 cut(s) 1012
MvnI CGCG 1 cut(s) 549
MwoI GCNNNNNNNGC 8 cut(s) 19, 36, 45, 137, 355, 372, 429, 543
NciI CCSGG 1 cut(s) 890
NcoI CCATGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 323
NdeII GATC 7 cut(s) 58, 197, 265, 481, 756, 809, 925
NlaIII CATG 5 cut(s) 52, 266, 275, 302, 823
NlaIV GGNNCC 2 cut(s) 585, 798
NmuCI GTSAC 1 cut(s) 1052
PagI TCATGA 1 cut(s) 262
PciSI GCTCTTC 1 cut(s) 288
PfeI GAWTC 3 cut(s) 611, 635, 824
PfoI TCCNGGA 1 cut(s) 1010
PinAI ACCGGT 1 cut(s) 640
PkrI GCNGC 4 cut(s) 133, 158, 548, 607
PleI GAGTC 3 cut(s) 244, 676, 1049
PpsI GAGTC 3 cut(s) 244, 676, 1049
PshBI ATTAAT 1 cut(s) 765
Psp6I CCWGG 1 cut(s) 1010
PspGI CCWGG 1 cut(s) 1010
PspN4I GGNNCC 2 cut(s) 585, 798
PspPI GGNCC 1 cut(s) 302
PsuI RGATCY 1 cut(s) 756
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 4 cut(s) 179, 329, 386, 676
RsaNI GTAC 4 cut(s) 178, 328, 385, 675
RseI CAYNNNNRTG 3 cut(s) 82, 261, 374
SapI GCTCTTC 1 cut(s) 288
SaqAI TTAA 3 cut(s) 9, 747, 765
SatI GCNGC 4 cut(s) 132, 157, 547, 606
Sau3AI GATC 7 cut(s) 58, 197, 265, 481, 756, 809, 925
Sau96I GGNCC 1 cut(s) 302
ScaI AGTACT 2 cut(s) 179, 676
SchI GAGTC 3 cut(s) 244, 677, 1049
ScrFI CCNGG 2 cut(s) 890, 1012
SfaNI GCATC 2 cut(s) 839, 977
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 3 cut(s) 82, 261, 374
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
Sse9I AATT 6 cut(s) 5, 532, 696, 900, 932, 993
SsiI CCGC 4 cut(s) 156, 334, 547, 854
SspMI CTAG 1 cut(s) 653
StyD4I CCNGG 2 cut(s) 888, 1010
StyI CCWWGG 1 cut(s) 819
TaaI ACNGT 3 cut(s) 74, 340, 1052
TaqI TCGA 2 cut(s) 408, 645
TaqII GACCGA 1 cut(s) 332
TasI AATT 6 cut(s) 5, 532, 696, 900, 932, 993
TatI WGTACW 3 cut(s) 177, 384, 674
TauI GCSGC 2 cut(s) 159, 549
TfiI GAWTC 3 cut(s) 611, 635, 824
Tru1I TTAA 3 cut(s) 9, 747, 765
Tru9I TTAA 3 cut(s) 9, 747, 765
TscAI CASTG 1 cut(s) 1074
TseFI GTSAC 1 cut(s) 1052
TseI GCWGC 2 cut(s) 131, 605
Tsp45I GTSAC 1 cut(s) 1052
TspDTI ATGAA 6 cut(s) 65, 97, 245, 306, 482, 708
TspRI CASTG 1 cut(s) 1074
VpaK11BI GGWCC 1 cut(s) 302
VspI ATTAAT 1 cut(s) 765
XapI RAATTY 2 cut(s) 5, 696
XbaI TCTAGA 1 cut(s) 652
XspI CTAG 1 cut(s) 653
ZrmI AGTACT 2 cut(s) 179, 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.