RchiOBHm_Chr6g0299531

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0017257
Physical Location & Seq
Forward (+)
60619954 .. 60621465
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
ATGCCAGTAAAATGTCCTCATTTTGTCAAAGTGATGCTTCCATCACATGTTGCTGGAGGCTTCTGGCTGGGTCTTCCTTCGACATTCTGCCACAAACATATAGGAACAAGCGACCAGACGATTATATTGGAAGATGAAACTGGCGATAAGTTCAAAACAGCATATCTCGGTCATAAGATGGGACTGAGTGCTGGATGGAGAGGGTTTTCCATTCATCACCATTTGCTTGAGGGAGATGTTTTAGTCTTCCAAAAAGTCAGGGCTAATGAATTTAAGGTAAATATATATATGATTCAGTCCTTCAATTGCTTGTTTAGATCCATGGCTGTATATTGTTTGATTTTGAGAGTGACATTTGAAGAAGAAGATATGTTCAATTATATTGACAATTATGAAGCCGGTGCAGAGAGTGAAGATGATATTGACCCTGAATTTTTCAAAGGAGCTGGGCTTTCGGAGTCTTCTGTCACTTGTTTTAAAGAAGTGACATGCATCGAGAAGTTCAAGATCATATTCAATGGCTGTAACATCTTGGTTCCTAAACTCGTCCTGAAGAAATATTATGAGCTTTGCCGCAGCCAAAACTCATACCTGCATGAAAATCTTGTGGAGTCTCTTAACAGCCAACTGGTTGCTGGACTAATCTCTGAGACGGTCAATATTGCAGATGCAATTAAATTCTGCAAGATTACCACCGAGGAGTCTGAGTTTTCGACGTGGGACAAGACCTTGCAAGCCTGTGAGATGTTAGGCATGAATGTGGGCTTCATGCGCAGCCGAGCGAGCAAGCTTGCAAGCCTTGCGGCAGAATCAAAGACGTACAAAGACGCGAGACTTGCAAGAGAGCAAGAAGAAGCAGAGTTTCGGGTCAGGTTGGCTGACTTTTTGAAAGAAAAAGATAAAGCAGATCGGGAGGGGTCTGAGAAATCAGTGCAGGTTGCTACAAGTTTCAAACAAGTGACAAATGTACCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

325

Amino Acids

36.91

Weight (kDa)

5.79

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 8 - 96 4e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017257)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41030 FvH4_2g41030
rosa_chinensis RchiOBHm_Chr6g0299531
rosa_laevigata RLG00000011418
rosa_multiflora Rmu_sc0004064.1_g000007
rosa_roxburghii Rroxscaffold_7G00168580
rosa_samantha Rh6AG400900 Rh6AG401000 Rh6BG409500 Rh6CG414900 Rh6DG402000 Rh6DG402100
rosa_wichuraiana Rw6G035090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 773
Acc36I ACCTGC 2 cut(s) 600, 925
AccII CGCG 1 cut(s) 830
AciI CCGC 2 cut(s) 574, 803
AclWI GGATC 1 cut(s) 312
AcsI RAATTY 3 cut(s) 269, 431, 677
AcuI CTGAAG 1 cut(s) 572
AfaI GTAC 2 cut(s) 821, 969
AflIII ACRYGT 1 cut(s) 46
AgsI TTSAA 9 cut(s) 154, 304, 359, 376, 439, 505, 517, 889, 952
AjiI CACGTC 1 cut(s) 717
AluBI AGCT 3 cut(s) 446, 568, 790
AluI AGCT 3 cut(s) 446, 568, 790
Alw26I GTCTC 3 cut(s) 618, 644, 826
AlwI GGATC 1 cut(s) 312
ApeKI GCWGC 2 cut(s) 576, 774
ApoI RAATTY 3 cut(s) 269, 431, 677
AspLEI GCGC 1 cut(s) 774
AsuHPI GGTGA 1 cut(s) 209
BaeI ACNNNNGTAYC 1 cut(s) 951
BbsI GAAGAC 3 cut(s) 65, 238, 453
BbvI GCAGC 2 cut(s) 588, 786
BccI CCATC 3 cut(s) 49, 172, 189
BcoDI GTCTC 3 cut(s) 618, 644, 826
BfuAI ACCTGC 2 cut(s) 600, 925
BisI GCNGC 4 cut(s) 574, 577, 775, 804
BlsI GCNGC 4 cut(s) 575, 578, 776, 805
BmgBI CACGTC 1 cut(s) 717
BmiI GGNNCC 1 cut(s) 537
BmsI GCATC 3 cut(s) 24, 501, 658
BpiI GAAGAC 3 cut(s) 65, 238, 453
BpmI CTGGAG 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 248
BsaJI CCNNGG 3 cut(s) 321, 696, 971
BsaXI ACNNNNNCTCC 2 cut(s) 692, 722
Bse118I RCCGGY 1 cut(s) 398
Bse1I ACTGG 3 cut(s) 5, 145, 633
BseDI CCNNGG 3 cut(s) 321, 696, 971
BseGI GGATG 1 cut(s) 200
BseMII CTCAG 4 cut(s) 176, 639, 696, 912
BseNI ACTGG 3 cut(s) 5, 145, 633
BseRI GAGGAG 1 cut(s) 713
BseXI GCAGC 2 cut(s) 588, 786
BseYI CCCAGC 2 cut(s) 67, 446
BsgI GTGCAG 2 cut(s) 423, 953
Bsh1236I CGCG 1 cut(s) 830
BsiSI CCGG 1 cut(s) 399
BslFI GGGAC 2 cut(s) 195, 734
BsmAI GTCTC 3 cut(s) 618, 644, 826
BsmBI CGTCTC 1 cut(s) 644
BsmFI GGGAC 2 cut(s) 195, 734
Bsp143I GATC 3 cut(s) 317, 507, 907
Bsp19I CCATGG 1 cut(s) 321
BspACI CCGC 2 cut(s) 574, 803
BspCNI CTCAG 4 cut(s) 177, 640, 697, 913
BspFNI CGCG 1 cut(s) 830
BspLI GGNNCC 1 cut(s) 537
BspMI ACCTGC 2 cut(s) 600, 925
BspPI GGATC 1 cut(s) 312
BsrFI RCCGGY 1 cut(s) 398
BsrI ACTGG 3 cut(s) 5, 145, 633
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 3 cut(s) 321, 696, 971
BssMI GATC 3 cut(s) 317, 507, 907
BssT1I CCWWGG 2 cut(s) 321, 971
Bst4CI ACNGT 1 cut(s) 655
BstAPI GCANNNNNTGC 1 cut(s) 800
BstC8I GCNNGC 5 cut(s) 735, 784, 788, 792, 796
BstDEI CTNAG 4 cut(s) 185, 648, 705, 921
BstDSI CCRYGG 1 cut(s) 321
BstF5I GGATG 1 cut(s) 200
BstFNI CGCG 1 cut(s) 830
BstHHI GCGC 1 cut(s) 774
BstKTI GATC 3 cut(s) 320, 510, 910
BstMAI GTCTC 3 cut(s) 618, 644, 826
BstMBI GATC 3 cut(s) 317, 507, 907
BstMWI GCNNNNNNNGC 4 cut(s) 771, 783, 800, 836
BstNSI RCATGY 2 cut(s) 50, 492
BstUI CGCG 1 cut(s) 830
BstV1I GCAGC 2 cut(s) 588, 786
BstV2I GAAGAC 3 cut(s) 65, 238, 453
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BtgI CCRYGG 1 cut(s) 321
BtrI CACGTC 1 cut(s) 717
BtsCI GGATG 1 cut(s) 200
BtsIMutI CAGTG 1 cut(s) 936
BveI ACCTGC 2 cut(s) 600, 925
Cac8I GCNNGC 5 cut(s) 735, 784, 788, 792, 796
CfoI GCGC 1 cut(s) 774
Cfr10I RCCGGY 1 cut(s) 398
CseI GACGC 1 cut(s) 836
Csp6I GTAC 2 cut(s) 820, 968
CviAII CATG 6 cut(s) 47, 322, 489, 596, 754, 769
CviQI GTAC 2 cut(s) 820, 968
DdeI CTNAG 4 cut(s) 185, 648, 705, 921
DpnI GATC 3 cut(s) 319, 509, 909
DpnII GATC 3 cut(s) 317, 507, 907
DraI TTTAAA 1 cut(s) 478
Eco130I CCWWGG 2 cut(s) 321, 971
Eco57I CTGAAG 1 cut(s) 572
EcoT14I CCWWGG 2 cut(s) 321, 971
EcoT22I ATGCAT 1 cut(s) 494
ErhI CCWWGG 2 cut(s) 321, 971
Esp3I CGTCTC 1 cut(s) 644
FaeI CATG 6 cut(s) 50, 325, 492, 599, 757, 772
FalI AAGNNNNNCTT 2 cut(s) 21, 53
FaqI GGGAC 2 cut(s) 195, 734
FatI CATG 6 cut(s) 46, 321, 488, 595, 753, 768
Fnu4HI GCNGC 4 cut(s) 574, 577, 775, 804
FokI GGATG 1 cut(s) 207
Fsp4HI GCNGC 4 cut(s) 574, 577, 775, 804
FspI TGCGCA 1 cut(s) 773
GlaI GCGC 1 cut(s) 773
GluI GCNGC 4 cut(s) 574, 577, 775, 804
GsaI CCCAGC 2 cut(s) 71, 450
GsuI CTGGAG 1 cut(s) 75
HapII CCGG 1 cut(s) 399
HgaI GACGC 1 cut(s) 836
HhaI GCGC 1 cut(s) 774
Hin1II CATG 6 cut(s) 50, 325, 492, 599, 757, 772
Hin6I GCGC 1 cut(s) 772
HinP1I GCGC 1 cut(s) 772
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 5 cut(s) 292, 458, 611, 701, 809
HpaII CCGG 1 cut(s) 399
HphI GGTGA 1 cut(s) 209
Hpy188I TCNGA 4 cut(s) 457, 649, 706, 922
Hpy188III TCNNGA 4 cut(s) 496, 505, 550, 911
Hpy99I CGWCG 1 cut(s) 718
HpyAV CCTTC 2 cut(s) 87, 310
HpyCH4III ACNGT 1 cut(s) 655
HpyCH4IV ACGT 2 cut(s) 716, 818
HpyF10VI GCNNNNNNNGC 4 cut(s) 771, 783, 800, 836
HpyF3I CTNAG 4 cut(s) 185, 648, 705, 921
HpySE526I ACGT 2 cut(s) 716, 818
Hsp92II CATG 6 cut(s) 50, 325, 492, 599, 757, 772
HspAI GCGC 1 cut(s) 772
Kzo9I GATC 3 cut(s) 317, 507, 907
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 2 cut(s) 588, 786
LweI GCATC 3 cut(s) 24, 501, 658
MaeII ACGT 2 cut(s) 716, 818
MaeIII GTNAC 5 cut(s) 349, 466, 484, 524, 958
MalI GATC 3 cut(s) 319, 509, 909
MboI GATC 3 cut(s) 317, 507, 907
MfeI CAATTG 1 cut(s) 304
MflI RGATCY 1 cut(s) 317
MluCI AATT 7 cut(s) 269, 304, 376, 388, 431, 672, 677
MlyI GAGTC 3 cut(s) 467, 620, 710
MnlI CCTC 6 cut(s) 27, 50, 194, 223, 691, 907
Mph1103I ATGCAT 1 cut(s) 494
MseI TTAA 4 cut(s) 273, 477, 618, 675
MslI CAYNNNNRTG 1 cut(s) 758
MspI CCGG 1 cut(s) 399
MunI CAATTG 1 cut(s) 304
MvnI CGCG 1 cut(s) 830
MwoI GCNNNNNNNGC 4 cut(s) 771, 783, 800, 836
NcoI CCATGG 1 cut(s) 321
NdeII GATC 3 cut(s) 317, 507, 907
NlaIII CATG 6 cut(s) 50, 325, 492, 599, 757, 772
NlaIV GGNNCC 1 cut(s) 537
NmeAIII GCCGAG 1 cut(s) 803
NmuCI GTSAC 4 cut(s) 349, 466, 484, 958
NsbI TGCGCA 1 cut(s) 773
NsiI ATGCAT 1 cut(s) 494
NspI RCATGY 2 cut(s) 50, 492
PciI ACATGT 1 cut(s) 46
PfeI GAWTC 2 cut(s) 292, 809
PkrI GCNGC 4 cut(s) 575, 578, 776, 805
PleI GAGTC 3 cut(s) 466, 619, 709
PpsI GAGTC 3 cut(s) 466, 619, 709
PscI ACATGT 1 cut(s) 46
PspFI CCCAGC 2 cut(s) 67, 446
PspN4I GGNNCC 1 cut(s) 537
PsuI RGATCY 1 cut(s) 317
RsaI GTAC 2 cut(s) 821, 969
RsaNI GTAC 2 cut(s) 820, 968
RseI CAYNNNNRTG 1 cut(s) 758
SaqAI TTAA 4 cut(s) 273, 477, 618, 675
SatI GCNGC 4 cut(s) 574, 577, 775, 804
Sau3AI GATC 3 cut(s) 317, 507, 907
SchI GAGTC 3 cut(s) 467, 620, 710
SfaNI GCATC 3 cut(s) 24, 501, 658
SmiMI CAYNNNNRTG 1 cut(s) 758
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 7 cut(s) 269, 304, 376, 388, 431, 672, 677
SsiI CCGC 2 cut(s) 574, 803
SspI AATATT 2 cut(s) 560, 661
StyI CCWWGG 2 cut(s) 321, 971
TaaI ACNGT 1 cut(s) 655
TaiI ACGT 2 cut(s) 719, 821
TaqI TCGA 3 cut(s) 80, 495, 713
TaqII GACCGA 1 cut(s) 158
TasI AATT 7 cut(s) 269, 304, 376, 388, 431, 672, 677
TauI GCSGC 2 cut(s) 576, 806
TfiI GAWTC 2 cut(s) 292, 809
Tru1I TTAA 4 cut(s) 273, 477, 618, 675
Tru9I TTAA 4 cut(s) 273, 477, 618, 675
TscAI CASTG 1 cut(s) 936
TseFI GTSAC 4 cut(s) 349, 466, 484, 958
TseI GCWGC 2 cut(s) 576, 774
Tsp45I GTSAC 4 cut(s) 349, 466, 484, 958
TspDTI ATGAA 7 cut(s) 150, 203, 282, 408, 612, 757, 770
TspRI CASTG 1 cut(s) 936
XapI RAATTY 3 cut(s) 269, 431, 677
XceI RCATGY 2 cut(s) 50, 492
XcmI CCANNNNNNNNNTGG 1 cut(s) 632
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.