RchiOBHm_Chr5g0006741

Endonuclease 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007730
Physical Location & Seq
Forward (+)
4195490 .. 4198596
3107 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 894 bp
ATGGGTTTGTTTGGGCTGTGGTGGAATGGAAGTGCGGTAGGGTTTCTTCTATTGGTGCCTGGAATTCTGGGTTGGGGCAAAGAAGGCCATTATGCAGTTTGCAAGATAGCAGAGGGATTTCTGAGTGAAGATGCTTTAGCTGCAGTGAAAGAATTGCTACCGGAATCTGCTGGAGGTGAACTTGCTGCAGTTTGCTCCTGGCCTGATGAGATTCGCTTTCATTATCATTGGAGTAGTGCTTTACACTATGTTGACACACCCGATTTTCTGTGTAACTACAATTACAGCAGAGACTGCCATGACTCTGCTGGACATAAAGGGAGATGTGTAACTGCAGCAATATACAACTATACGATGCAACTTTTGACTTATCAGGATTCCATCTCAAAATCAAAATACAACTTAACAGAGGCACTTCTTTTCTTGTCTCACTTTATTGGGGATGTCCATCAGCCCCTACATGTTGGTTTCACTGGAGATCTAGGGGGGAACACAATAATAGTCCGTTGGTATCGCAGGAAGACAAATCTTCACCATGTGTGGGATAATATGATCATTGAATCTGCTGTAAAGACAATGTACAGTGAGGATCTTGGACTCATGATAGAAGCCATTCAGAGGAATATTACGGATGCTTGGTTCAATGATATGTCATCATTGCAGATTTGTACAAATAATCGGACAACTGTATGCCCAAATGAGTATGCTTCTGAAAGCATTCGTTTAGCATGCAAATTTGCTTACAGGAATGCCACTCCAGGAAGCACTCTGAATGATGATTACTTTCTTTCTCGGCTACCTGTCGTGGAGAAGCGGCTAGCTCAAAGTGGGGTTCGCTTGGCTGCTACCCTCAACCATATCTTCTCTTCTGAACCAAAAATTGCTGAAGCATGA

Protein Analysis

297

Amino Acids

33.31

Weight (kDa)

5.93

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 25 - 288 1.4e-82 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 55
AciI CCGC 2 cut(s) 35, 814
AclWI GGATC 1 cut(s) 597
AcsI RAATTY 2 cut(s) 63, 734
AdeI CACNNNGTG 1 cut(s) 538
AfaI GTAC 2 cut(s) 581, 670
AfiI CCNNNNNNNGG 2 cut(s) 541, 618
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 2 cut(s) 560, 643
AjnI CCWGG 3 cut(s) 58, 197, 757
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AloI GAACNNNNNNTCC 2 cut(s) 623, 655
AluBI AGCT 2 cut(s) 140, 821
AluI AGCT 2 cut(s) 140, 821
Alw26I GTCTC 2 cut(s) 285, 432
AlwI GGATC 1 cut(s) 597
AlwNI CAGNNNCTG 1 cut(s) 294
AoxI GGCC 2 cut(s) 85, 200
ApeKI GCWGC 4 cut(s) 140, 185, 335, 842
ApoI RAATTY 2 cut(s) 63, 734
Asp700I GAANNNNTTC 2 cut(s) 612, 717
AsuHPI GGTGA 2 cut(s) 188, 524
AsuNHI GCTAGC 1 cut(s) 817
BanI GGYRCC 1 cut(s) 55
BarI GAAGNNNNNNTAC 2 cut(s) 30, 62
BbsI GAAGAC 1 cut(s) 527
BbvI GCAGC 4 cut(s) 127, 172, 347, 829
BccI CCATC 2 cut(s) 389, 456
BciT130I CCWGG 3 cut(s) 60, 199, 759
BclI TGATCA 1 cut(s) 552
BcoDI GTCTC 2 cut(s) 285, 432
BfaI CTAG 2 cut(s) 482, 818
BfmI CTRYAG 3 cut(s) 141, 186, 333
BglII AGATCT 1 cut(s) 478
BisI GCNGC 5 cut(s) 141, 186, 336, 815, 843
BlsI GCNGC 5 cut(s) 142, 187, 337, 816, 844
Bme1390I CCNGG 3 cut(s) 60, 199, 759
BmiI GGNNCC 1 cut(s) 57
BmrFI CCNGG 3 cut(s) 60, 199, 759
BmsI GCATC 3 cut(s) 121, 345, 622
BmtI GCTAGC 1 cut(s) 821
BpiI GAAGAC 1 cut(s) 527
BpmI CTGGAG 3 cut(s) 192, 495, 741
BsaBI GATNNNNATC 1 cut(s) 447
BsaWI WCCGGW 1 cut(s) 160
Bsc4I CCNNNNNNNGG 2 cut(s) 541, 618
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 656
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 3 cut(s) 60, 199, 759
BseGI GGATG 2 cut(s) 448, 637
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 2 cut(s) 541, 618
BseMI GCAATG 1 cut(s) 656
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 1 cut(s) 478
BseXI GCAGC 4 cut(s) 127, 172, 347, 829
BshFI GGCC 2 cut(s) 87, 202
BshNI GGYRCC 1 cut(s) 55
BsiSI CCGG 1 cut(s) 161
BslI CCNNNNNNNGG 2 cut(s) 541, 618
BsmAI GTCTC 2 cut(s) 285, 432
BsmI GAATGC 2 cut(s) 717, 754
BsnI GGCC 2 cut(s) 87, 202
Bsp1407I TGTACA 2 cut(s) 579, 668
Bsp143I GATC 3 cut(s) 478, 552, 589
BspACI CCGC 2 cut(s) 35, 814
BspANI GGCC 2 cut(s) 87, 202
BspCNI CTCAG 1 cut(s) 114
BspHI TCATGA 1 cut(s) 600
BspLI GGNNCC 1 cut(s) 57
BspMAI CTGCAG 3 cut(s) 145, 190, 337
BspOI GCTAGC 1 cut(s) 821
BspPI GGATC 1 cut(s) 597
BspT107I GGYRCC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 656
BsrGI TGTACA 2 cut(s) 579, 668
BsrI ACTGG 1 cut(s) 478
BssMI GATC 3 cut(s) 478, 552, 589
Bst2UI CCWGG 3 cut(s) 60, 199, 759
Bst4CI ACNGT 2 cut(s) 584, 688
Bst6I CTCTTC 1 cut(s) 871
BstAPI GCANNNNNTGC 1 cut(s) 294
BstAUI TGTACA 2 cut(s) 579, 668
BstC8I GCNNGC 2 cut(s) 730, 819
BstDEI CTNAG 1 cut(s) 122
BstF5I GGATG 2 cut(s) 448, 637
BstKTI GATC 3 cut(s) 481, 555, 592
BstMAI GTCTC 2 cut(s) 285, 432
BstMBI GATC 3 cut(s) 478, 552, 589
BstMWI GCNNNNNNNGC 3 cut(s) 84, 140, 294
BstNI CCWGG 3 cut(s) 60, 199, 759
BstNSI RCATGY 2 cut(s) 464, 732
BstSCI CCNGG 3 cut(s) 58, 197, 757
BstSFI CTRYAG 3 cut(s) 141, 186, 333
BstV1I GCAGC 4 cut(s) 127, 172, 347, 829
BstV2I GAAGAC 1 cut(s) 527
BstX2I RGATCY 2 cut(s) 478, 589
BstYI RGATCY 2 cut(s) 478, 589
BsuRI GGCC 2 cut(s) 87, 202
BtsCI GGATG 2 cut(s) 448, 637
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 3 cut(s) 150, 471, 589
Cac8I GCNNGC 2 cut(s) 730, 819
CaiI CAGNNNCTG 1 cut(s) 294
CciI TCATGA 1 cut(s) 600
Csp6I GTAC 2 cut(s) 580, 669
CviAII CATG 6 cut(s) 299, 461, 536, 601, 729, 891
CviQI GTAC 2 cut(s) 580, 669
DdeI CTNAG 1 cut(s) 122
DpnI GATC 3 cut(s) 480, 554, 591
DpnII GATC 3 cut(s) 478, 552, 589
DraIII CACNNNGTG 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 871
EarI CTCTTC 1 cut(s) 871
EcoRI GAATTC 1 cut(s) 63
EcoRII CCWGG 3 cut(s) 58, 197, 757
FaeI CATG 6 cut(s) 302, 464, 539, 604, 732, 894
FatI CATG 6 cut(s) 298, 460, 535, 600, 728, 890
FbaI TGATCA 1 cut(s) 552
Fnu4HI GCNGC 5 cut(s) 141, 186, 336, 815, 843
FokI GGATG 2 cut(s) 455, 644
Fsp4HI GCNGC 5 cut(s) 141, 186, 336, 815, 843
FspBI CTAG 2 cut(s) 482, 818
GluI GCNGC 5 cut(s) 141, 186, 336, 815, 843
GsuI CTGGAG 3 cut(s) 192, 495, 741
HaeIII GGCC 2 cut(s) 87, 202
HapII CCGG 1 cut(s) 161
Hin1II CATG 6 cut(s) 302, 464, 539, 604, 732, 894
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 6 cut(s) 164, 211, 302, 377, 560, 597
HpaII CCGG 1 cut(s) 161
HphI GGTGA 2 cut(s) 188, 524
Hpy166II GTNNAC 2 cut(s) 179, 253
Hpy188I TCNGA 6 cut(s) 123, 618, 681, 712, 771, 871
Hpy188III TCNNGA 2 cut(s) 374, 601
Hpy8I GTNNAC 2 cut(s) 179, 253
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 2 cut(s) 584, 688
HpyCH4V TGCA 8 cut(s) 95, 102, 143, 188, 335, 358, 661, 732
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 140, 294
HpyF3I CTNAG 1 cut(s) 122
Hsp92II CATG 6 cut(s) 302, 464, 539, 604, 732, 894
Ksp22I TGATCA 1 cut(s) 552
Kzo9I GATC 3 cut(s) 478, 552, 589
LmnI GCTCC 1 cut(s) 200
Lsp1109I GCAGC 4 cut(s) 127, 172, 347, 829
LweI GCATC 3 cut(s) 121, 345, 622
MaeI CTAG 2 cut(s) 482, 818
MaeIII GTNAC 2 cut(s) 272, 328
MalI GATC 3 cut(s) 480, 554, 591
MboI GATC 3 cut(s) 478, 552, 589
MboII GAAGA 6 cut(s) 38, 140, 521, 532, 853, 858
MflI RGATCY 2 cut(s) 478, 589
MluCI AATT 5 cut(s) 63, 152, 280, 734, 879
MlyI GAGTC 2 cut(s) 296, 591
MnlI CCTC 6 cut(s) 106, 167, 403, 580, 612, 860
MroXI GAANNNNTTC 2 cut(s) 612, 717
MseI TTAA 1 cut(s) 404
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 3 cut(s) 60, 199, 759
Mva1269I GAATGC 2 cut(s) 717, 754
MvaI CCWGG 3 cut(s) 60, 199, 759
MwoI GCNNNNNNNGC 3 cut(s) 84, 140, 294
NdeII GATC 3 cut(s) 478, 552, 589
NheI GCTAGC 1 cut(s) 817
NlaIII CATG 6 cut(s) 302, 464, 539, 604, 732, 894
NlaIV GGNNCC 1 cut(s) 57
NmeAIII GCCGAG 1 cut(s) 772
NspI RCATGY 2 cut(s) 464, 732
PaeI GCATGC 1 cut(s) 732
PagI TCATGA 1 cut(s) 600
PciI ACATGT 1 cut(s) 460
PctI GAATGC 2 cut(s) 717, 754
PdmI GAANNNNTTC 2 cut(s) 612, 717
PfeI GAWTC 4 cut(s) 164, 211, 377, 560
PfoI TCCNGGA 1 cut(s) 757
PkrI GCNGC 5 cut(s) 142, 187, 337, 816, 844
PleI GAGTC 2 cut(s) 296, 591
PpsI GAGTC 2 cut(s) 296, 591
PscI ACATGT 1 cut(s) 460
Psp6I CCWGG 3 cut(s) 58, 197, 757
PspGI CCWGG 3 cut(s) 58, 197, 757
PspN4I GGNNCC 1 cut(s) 57
PstI CTGCAG 3 cut(s) 145, 190, 337
PstNI CAGNNNCTG 1 cut(s) 294
PsuI RGATCY 2 cut(s) 478, 589
RsaI GTAC 2 cut(s) 581, 670
RsaNI GTAC 2 cut(s) 580, 669
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 5 cut(s) 141, 186, 336, 815, 843
Sau3AI GATC 3 cut(s) 478, 552, 589
SchI GAGTC 2 cut(s) 296, 591
ScrFI CCNGG 3 cut(s) 60, 199, 759
SetI ASST 4 cut(s) 142, 178, 802, 823
SfaNI GCATC 3 cut(s) 121, 345, 622
SfcI CTRYAG 3 cut(s) 141, 186, 333
SphI GCATGC 1 cut(s) 732
Sse9I AATT 5 cut(s) 63, 152, 280, 734, 879
SsiI CCGC 2 cut(s) 35, 814
SspI AATATT 1 cut(s) 625
SspMI CTAG 2 cut(s) 482, 818
StyD4I CCNGG 3 cut(s) 58, 197, 757
TaaI ACNGT 2 cut(s) 584, 688
TasI AATT 5 cut(s) 63, 152, 280, 734, 879
TatI WGTACW 2 cut(s) 579, 668
TauI GCSGC 1 cut(s) 817
TfiI GAWTC 4 cut(s) 164, 211, 377, 560
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TscAI CASTG 3 cut(s) 150, 478, 589
TseI GCWGC 4 cut(s) 140, 185, 335, 842
TspDTI ATGAA 1 cut(s) 209
TspGWI ACGGA 2 cut(s) 494, 644
TspRI CASTG 3 cut(s) 150, 478, 589
XapI RAATTY 2 cut(s) 63, 734
XceI RCATGY 2 cut(s) 464, 732
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XmnI GAANNNNTTC 2 cut(s) 612, 717
XspI CTAG 2 cut(s) 482, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.