RchiOBHm_Chr5g0019591

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006563
Physical Location & Seq
Forward (+)
14060724 .. 14062134
1411 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1338 bp
ATGGACCGGTCTCCTTGTTGCAAGCATTTCAGATTACTTGGTTGCAACATGAATTCAGTAGCCTGCAAATGTGTGGTTTTAGTTATCATTGCTCTGGTTATTAGAGTTGTTCTGCTCCCTACATACTCTAGCTTTTATGGGACTGAGCAGAGCCATTTGGTGTTGGTCAGCAACCTCTCCTTCTCATCTTTGTCAGCCAGTACTAATTTCGGAATCCGAAAAGATAAGTTTCTCGAGGTTCCTCAAATTGTATGGGGATTAAATAATCAGAAAATTGCATTTGCAAGAGCTTGTCTAACCGCGAGGCTTATGAACCGGACTCTGTTGATGCCTAGCTTGAGTGCTTCCTTGTTCTACAAAGACGTTGAGCTCTTGAAGCCCATTTCGTTTCATAAGGTCTTCAAGTTTGACAAGTTCAATTCGCTCTGCAATGGCTATGTTCAGTTGGGGAAATACTCCGATCTTAAAAACCAAACAGGCGCGTTTGAGCTTCAGAAAGGAAGTGGAAGGAGGTGGACACCCGAGAGAGATTTGGATCAGTTGAAGCAGCATAGTAAGGAAGCATATGACGAACATGAGACGATTCGTATAGTTGGGAAGAACCCATTTTTGTGGCATGATCATTGGCCTGTGAAGGACTATGCAAAGGTGTTTGAGTGCTTGGTTTTGGTTGACGAGATCATGAGAGAAGCTGACAAAGTTGTGTCAAGGATTAGAGAGATAGGAGCAGAACAAGTGAGAAGCAAAAGCAAGTCTGCTGAGAACACTTTGTTGCTCAAGTCAGTGCCTTATGTAGCTGTTCATATGAGGATAGAGTTAGATTGGATGATACACTGTAAGAATCTTGAGCGTAGACTGAACATAACCGAAATTTGTAGCAGCAAAGAGGAGATCATGGAGAGAGTTGGCAACATTGTTGGCCTTAAGACTCCAGCTGTTGTATATTTAGCTGTGGCTGATAGTCTTCTTCAAGATCCATCAATTTTGAACGGATGGAAAGAAGGGTTGGTTCCTTTTGAGAAGAAGAAACTGGGTTTTGATGCACGTTACAAGAAGTTCCCCTATCTCATTCAGTCTGCGATTGACTATGAAGTGTGCTTGAGAGCCGATGTGTTTGTTGGAAACAGTTTCTCTACATTTTCTAGCCTCATTGCTCTTGAGAGAACCCAGAAGCTGATTAAAATGGGTGTCAATAGCTCATGTGGTGAAGATGTGAGGTGGCCTGCTTATGCGTACAACATATTAGGGGGATCAAAAGGGCCTCAAAAATGGATGACAAACATGTCTGGCTCAAGTCTTAAAGAAATTAGCTATGGCTCTAACCAAATTTCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

445

Amino Acids

50.52

Weight (kDa)

9.1

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 86 - 389 2.5e-25 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1282
AccI GTMKAC 1 cut(s) 853
AccII CGCG 2 cut(s) 302, 482
AciI CCGC 1 cut(s) 300
AclWI GGATC 3 cut(s) 543, 968, 1258
AcsI RAATTY 3 cut(s) 52, 870, 1326
AcuI CTGAAG 1 cut(s) 476
AfaI GTAC 2 cut(s) 202, 1235
AflII CTTAAG 1 cut(s) 923
AflIII ACRYGT 1 cut(s) 1281
AgeI ACCGGT 1 cut(s) 6
AgsI TTSAA 6 cut(s) 376, 403, 418, 544, 971, 988
AjuI GAANNNNNNNTTGG 2 cut(s) 989, 1021
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 2 cut(s) 15, 572
AlwI GGATC 3 cut(s) 543, 968, 1258
AlwNI CAGNNNCTG 1 cut(s) 1174
Ama87I CYCGRG 2 cut(s) 233, 521
AoxI GGCC 4 cut(s) 626, 919, 1220, 1259
ApeKI GCWGC 2 cut(s) 547, 879
ApoI RAATTY 3 cut(s) 52, 870, 1326
AsiGI ACCGGT 1 cut(s) 6
AspLEI GCGC 1 cut(s) 482
AspS9I GGNCC 2 cut(s) 4, 1259
AsuHPI GGTGA 1 cut(s) 1217
AvaI CYCGRG 2 cut(s) 233, 521
AvaII GGWCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 372
BbsI GAAGAC 2 cut(s) 391, 956
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 2 cut(s) 559, 891
BccI CCATC 2 cut(s) 985, 987
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 2 cut(s) 15, 572
BfaI CTAG 3 cut(s) 129, 333, 1143
BfrI CTTAAG 1 cut(s) 923
BisI GCNGC 2 cut(s) 548, 880
BlsI GCNGC 2 cut(s) 549, 881
BmcAI AGTACT 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 2 cut(s) 233, 521
BmgT120I GGNCC 2 cut(s) 4, 1259
BmiI GGNNCC 2 cut(s) 240, 1011
BmrI ACTGGG 1 cut(s) 1040
BmsI GCATC 2 cut(s) 318, 1030
BmuI ACTGGG 1 cut(s) 1040
BpiI GAAGAC 2 cut(s) 391, 956
BpmI CTGGAG 1 cut(s) 915
BpuEI CTTGAG 6 cut(s) 358, 761, 866, 1120, 1178, 1276
BsaBI GATNNNNATC 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 15
BsaWI WCCGGW 2 cut(s) 6, 315
Bse118I RCCGGY 1 cut(s) 6
Bse1I ACTGG 2 cut(s) 198, 1035
Bse3DI GCAATG 3 cut(s) 87, 436, 1149
Bse8I GATNNNNATC 1 cut(s) 534
BseGI GGATG 3 cut(s) 831, 998, 1278
BseJI GATNNNNATC 1 cut(s) 534
BseMI GCAATG 3 cut(s) 87, 436, 1149
BseMII CTCAG 2 cut(s) 135, 750
BseNI ACTGG 2 cut(s) 198, 1035
BseRI GAGGAG 1 cut(s) 902
BseXI GCAGC 2 cut(s) 559, 891
Bsh1236I CGCG 2 cut(s) 302, 482
BshFI GGCC 4 cut(s) 628, 921, 1222, 1261
BshTI ACCGGT 1 cut(s) 6
BsiHKAI GWGCWC 1 cut(s) 372
BsiHKCI CYCGRG 2 cut(s) 233, 521
BsiSI CCGG 2 cut(s) 7, 316
BslFI GGGAC 1 cut(s) 154
BsmAI GTCTC 2 cut(s) 15, 572
BsmBI CGTCTC 1 cut(s) 572
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 4 cut(s) 628, 921, 1222, 1261
Bso31I GGTCTC 1 cut(s) 15
BsoBI CYCGRG 2 cut(s) 233, 521
Bsp1286I GDGCHC 1 cut(s) 372
Bsp143I GATC 7 cut(s) 460, 535, 619, 678, 891, 973, 1250
BspACI CCGC 1 cut(s) 300
BspANI GGCC 4 cut(s) 628, 921, 1222, 1261
BspCNI CTCAG 2 cut(s) 136, 751
BspFNI CGCG 2 cut(s) 302, 482
BspHI TCATGA 1 cut(s) 681
BspLI GGNNCC 2 cut(s) 240, 1011
BspPI GGATC 3 cut(s) 543, 968, 1258
BspTI CTTAAG 1 cut(s) 923
BspTNI GGTCTC 1 cut(s) 15
BsrDI GCAATG 3 cut(s) 87, 436, 1149
BsrFI RCCGGY 1 cut(s) 6
BsrI ACTGG 2 cut(s) 198, 1035
BssAI RCCGGY 1 cut(s) 6
BssMI GATC 7 cut(s) 460, 535, 619, 678, 891, 973, 1250
Bst4CI ACNGT 2 cut(s) 836, 1127
BstAFI CTTAAG 1 cut(s) 923
BstC8I GCNNGC 3 cut(s) 23, 64, 1224
BstDEI CTNAG 2 cut(s) 144, 759
BstF5I GGATG 3 cut(s) 831, 998, 1278
BstFNI CGCG 2 cut(s) 302, 482
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 7 cut(s) 463, 538, 622, 681, 894, 976, 1253
BstMAI GTCTC 2 cut(s) 15, 572
BstMBI GATC 7 cut(s) 460, 535, 619, 678, 891, 973, 1250
BstMWI GCNNNNNNNGC 1 cut(s) 376
BstNSI RCATGY 1 cut(s) 1285
BstUI CGCG 2 cut(s) 302, 482
BstV1I GCAGC 2 cut(s) 559, 891
BstV2I GAAGAC 2 cut(s) 391, 956
BstX2I RGATCY 1 cut(s) 973
BstXI CCANNNNNNTGG 1 cut(s) 612
BstYI RGATCY 1 cut(s) 973
BsuRI GGCC 4 cut(s) 628, 921, 1222, 1261
BtsCI GGATG 3 cut(s) 831, 998, 1278
BtsIMutI CAGTG 2 cut(s) 789, 832
Cac8I GCNNGC 3 cut(s) 23, 64, 1224
CaiI CAGNNNCTG 1 cut(s) 1174
CciI TCATGA 1 cut(s) 681
CfoI GCGC 1 cut(s) 482
Cfr10I RCCGGY 1 cut(s) 6
Cfr13I GGNCC 2 cut(s) 4, 1259
Csp6I GTAC 2 cut(s) 201, 1234
CspAI ACCGGT 1 cut(s) 6
CviAII CATG 8 cut(s) 49, 575, 617, 682, 895, 1200, 1282, 1332
CviQI GTAC 2 cut(s) 201, 1234
DdeI CTNAG 2 cut(s) 144, 759
DpnI GATC 7 cut(s) 462, 537, 621, 680, 893, 975, 1252
DpnII GATC 7 cut(s) 460, 535, 619, 678, 891, 973, 1250
DrdI GACNNNNNNGTC 1 cut(s) 1282
DseDI GACNNNNNNGTC 1 cut(s) 1282
Ecl136II GAGCTC 1 cut(s) 370
Eco24I GRGCYC 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 476
Eco88I CYCGRG 2 cut(s) 233, 521
EcoICRI GAGCTC 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 1259
EcoRI GAATTC 1 cut(s) 52
EcoT38I GRGCYC 1 cut(s) 372
Esp3I CGTCTC 1 cut(s) 572
FaeI CATG 8 cut(s) 52, 578, 620, 685, 898, 1203, 1285, 1335
FaqI GGGAC 1 cut(s) 154
FatI CATG 8 cut(s) 48, 574, 616, 681, 894, 1199, 1281, 1331
FauNDI CATATG 2 cut(s) 565, 804
FbaI TGATCA 1 cut(s) 619
FblI GTMKAC 1 cut(s) 853
Fnu4HI GCNGC 2 cut(s) 548, 880
FokI GGATG 3 cut(s) 838, 1005, 1285
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 2 cut(s) 548, 880
FspBI CTAG 3 cut(s) 129, 333, 1143
GlaI GCGC 1 cut(s) 481
GluI GCNGC 2 cut(s) 548, 880
GsuI CTGGAG 1 cut(s) 915
HaeIII GGCC 4 cut(s) 628, 921, 1222, 1261
HapII CCGG 2 cut(s) 7, 316
HhaI GCGC 1 cut(s) 482
Hin1II CATG 8 cut(s) 52, 578, 620, 685, 898, 1203, 1285, 1335
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HinfI GANTC 5 cut(s) 213, 319, 583, 841, 928
HpaII CCGG 2 cut(s) 7, 316
HphI GGTGA 1 cut(s) 1217
Hpy166II GTNNAC 3 cut(s) 516, 673, 854
Hpy188I TCNGA 6 cut(s) 32, 212, 218, 270, 460, 495
Hpy188III TCNNGA 6 cut(s) 233, 373, 682, 845, 971, 1157
Hpy8I GTNNAC 3 cut(s) 516, 673, 854
HpyAV CCTTC 4 cut(s) 190, 501, 628, 995
HpyCH4III ACNGT 2 cut(s) 836, 1127
HpyCH4IV ACGT 2 cut(s) 363, 1045
HpyCH4V TGCA 8 cut(s) 21, 45, 66, 278, 284, 429, 644, 1043
HpyF10VI GCNNNNNNNGC 1 cut(s) 376
HpyF3I CTNAG 2 cut(s) 144, 759
HpySE526I ACGT 2 cut(s) 363, 1045
Hsp92II CATG 8 cut(s) 52, 578, 620, 685, 898, 1203, 1285, 1335
HspAI GCGC 1 cut(s) 480
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 7 cut(s) 460, 535, 619, 678, 891, 973, 1250
LmnI GCTCC 2 cut(s) 120, 725
Lsp1109I GCAGC 2 cut(s) 559, 891
LweI GCATC 2 cut(s) 318, 1030
MaeI CTAG 3 cut(s) 129, 333, 1143
MaeII ACGT 2 cut(s) 363, 1045
MaeIII GTNAC 1 cut(s) 1046
MalI GATC 7 cut(s) 462, 537, 621, 680, 893, 975, 1252
MboI GATC 7 cut(s) 460, 535, 619, 678, 891, 973, 1250
MboII GAAGA 7 cut(s) 391, 610, 956, 959, 1033, 1036, 1220
MflI RGATCY 1 cut(s) 973
MhlI GDGCHC 1 cut(s) 372
MluCI AATT 9 cut(s) 52, 205, 246, 273, 418, 870, 981, 1305, 1326
MlyI GAGTC 2 cut(s) 313, 922
MmeI TCCRAC 1 cut(s) 1099
MseI TTAA 5 cut(s) 260, 465, 924, 1179, 1299
MslI CAYNNNNRTG 1 cut(s) 610
MspA1I CMGCKG 1 cut(s) 935
MspCI CTTAAG 1 cut(s) 923
MspI CCGG 2 cut(s) 7, 316
MvnI CGCG 2 cut(s) 302, 482
MwoI GCNNNNNNNGC 1 cut(s) 376
NdeI CATATG 2 cut(s) 565, 804
NdeII GATC 7 cut(s) 460, 535, 619, 678, 891, 973, 1250
NlaIII CATG 8 cut(s) 52, 578, 620, 685, 898, 1203, 1285, 1335
NlaIV GGNNCC 2 cut(s) 240, 1011
NspI RCATGY 1 cut(s) 1285
PaeR7I CTCGAG 1 cut(s) 233
PagI TCATGA 1 cut(s) 681
PciI ACATGT 1 cut(s) 1281
PfeI GAWTC 3 cut(s) 213, 583, 841
PinAI ACCGGT 1 cut(s) 6
PkrI GCNGC 2 cut(s) 549, 881
PleI GAGTC 2 cut(s) 313, 922
PpsI GAGTC 2 cut(s) 313, 922
PscI ACATGT 1 cut(s) 1281
Psp124BI GAGCTC 1 cut(s) 372
PspN4I GGNNCC 2 cut(s) 240, 1011
PspPI GGNCC 2 cut(s) 4, 1259
PstNI CAGNNNCTG 1 cut(s) 1174
PsuI RGATCY 1 cut(s) 973
PvuII CAGCTG 1 cut(s) 935
RsaI GTAC 2 cut(s) 202, 1235
RsaNI GTAC 2 cut(s) 201, 1234
RseI CAYNNNNRTG 1 cut(s) 610
SacI GAGCTC 1 cut(s) 372
SaqAI TTAA 5 cut(s) 260, 465, 924, 1179, 1299
SatI GCNGC 2 cut(s) 548, 880
Sau3AI GATC 7 cut(s) 460, 535, 619, 678, 891, 973, 1250
Sau96I GGNCC 2 cut(s) 4, 1259
ScaI AGTACT 1 cut(s) 202
SchI GAGTC 2 cut(s) 313, 922
SduI GDGCHC 1 cut(s) 372
SfaNI GCATC 2 cut(s) 318, 1030
Sfr274I CTCGAG 1 cut(s) 233
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 1 cut(s) 233
SmiMI CAYNNNNRTG 1 cut(s) 610
SmlI CTYRAG 8 cut(s) 233, 337, 776, 845, 923, 1099, 1157, 1291
SmoI CTYRAG 8 cut(s) 233, 337, 776, 845, 923, 1099, 1157, 1291
Sse9I AATT 9 cut(s) 52, 205, 246, 273, 418, 870, 981, 1305, 1326
SsiI CCGC 1 cut(s) 300
SspMI CTAG 3 cut(s) 129, 333, 1143
SstI GAGCTC 1 cut(s) 372
TaaI ACNGT 2 cut(s) 836, 1127
TaiI ACGT 2 cut(s) 366, 1048
TaqI TCGA 1 cut(s) 234
TasI AATT 9 cut(s) 52, 205, 246, 273, 418, 870, 981, 1305, 1326
TatI WGTACW 1 cut(s) 200
TfiI GAWTC 3 cut(s) 213, 583, 841
Tru1I TTAA 5 cut(s) 260, 465, 924, 1179, 1299
Tru9I TTAA 5 cut(s) 260, 465, 924, 1179, 1299
TscAI CASTG 2 cut(s) 789, 839
TseI GCWGC 2 cut(s) 547, 879
TspDTI ATGAA 6 cut(s) 65, 326, 380, 791, 1104, 1320
TspGWI ACGGA 1 cut(s) 1005
TspRI CASTG 2 cut(s) 789, 839
Vha464I CTTAAG 1 cut(s) 923
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 3 cut(s) 52, 870, 1326
XceI RCATGY 1 cut(s) 1285
XhoI CTCGAG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 853
XspI CTAG 3 cut(s) 129, 333, 1143
ZrmI AGTACT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.