RchiOBHm_Chr5g0052471

Lysosomal Pro-X

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0017520
Physical Location & Seq
Forward (+)
54407364 .. 54408687
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
ATGTGTATTAGATTTGGTAGAGATAAATCCTTCATGTATTACATTTGGTTTTTGGCAATTAGACCTTTGGAGGATGGAGATGAACTCGAGCTAAAGGCCTACTTGGAGTTCATTTATATAACTGCATCTCAGTATGATGACCCTCCAACATATCCAGTCAATCTGATCTGCAACAGTATAAATGCAGCAGCAGACAGAGGAGATGATATCATTGATCGAATTGCCTCGGCTGTTAATTATACTCGTCGAGGAGAGAATAGAGGTCAATGTTTGGACCTAAGTAGCGAACTTCGCCCATCAGATGATGCAGTGGATGGTTTGAACTGGTTATGGCAGGCATGTACTGAAATTTTTATGCCAAACGGTCGAGAAACGGACGAGACTATGTTTCAGGCAGAACCATTTGATGCGGATTCCTACACTCAAAGGTGCCACCAGTTATATCAAGGTGCCCGCCCTAGACCCCATTGGATCACAACTTATTATGGGGGGCATCATATAAGGATGACCTTGGAGAGGTCTGCCAGTAACATCATCTTCTCAAATGGCCAGCGTGATCCTTTTAGCGTTGCCGGAGTATTGACAAATATATCGCCCAGCATAGTAGCAGTGACTACACGTCAAGGTTCTCATTGCTTGGACTTATTACCTGCAAGGATAAATGATCCCAGGTGGTTAGTTGTGCAGCGACACATAGAAGTTGGGATTATCACAGGATGGATTCGCAACTACTACAATGATTTGCGTATCTTTTCTTCTGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04614 ko04974 map04614 map04974
Pfam Domains
Protein Families
Peptidase_S28

Protein Analysis

255

Amino Acids

29.36

Weight (kDa)

5.25

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 50 - 231 9.8e-22 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017520)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28680
fragaria_vesca FvH4_3g28620
rosa_chinensis RchiOBHm_Chr5g0052461 RchiOBHm_Chr5g0052471
rosa_laevigata RLG00000034817
rosa_multiflora Rmu_sc0006803.1_g000044
rosa_roxburghii Rroxscaffold_1G00028350
rosa_rugosa Rorug05G0271800
rosa_samantha Rh5BG355500 Rh5CG380900 Rh5DG370700
rosa_wichuraiana Rw5G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 658
AccB1I GGYRCC 2 cut(s) 429, 449
AciI CCGC 2 cut(s) 410, 454
AclWI GGATC 3 cut(s) 479, 551, 659
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 1 cut(s) 348
AfaI GTAC 1 cut(s) 343
AfiI CCNNNNNNNGG 1 cut(s) 516
AflIII ACRYGT 1 cut(s) 617
AgsI TTSAA 1 cut(s) 322
AhdI GACNNNNNGTC 1 cut(s) 618
AjiI CACGTC 1 cut(s) 620
AjnI CCWGG 1 cut(s) 668
AluBI AGCT 1 cut(s) 91
AluI AGCT 1 cut(s) 91
Alw26I GTCTC 1 cut(s) 374
AlwI GGATC 3 cut(s) 479, 551, 659
Ama87I CYCGRG 1 cut(s) 86
AoxI GGCC 2 cut(s) 96, 547
ApeKI GCWGC 3 cut(s) 185, 188, 685
ApoI RAATTY 1 cut(s) 348
AspS9I GGNCC 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 1 cut(s) 274
BaeGI GKGCMC 1 cut(s) 454
BalI TGGCCA 1 cut(s) 549
BanI GGYRCC 2 cut(s) 429, 449
BarI GAAGNNNNNNTAC 1 cut(s) 739
BbvI GCAGC 3 cut(s) 197, 200, 697
BccI CCATC 4 cut(s) 68, 304, 308, 711
BciT130I CCWGG 1 cut(s) 670
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 1 cut(s) 459
BfuAI ACCTGC 1 cut(s) 658
BisI GCNGC 3 cut(s) 186, 189, 686
BlsI GCNGC 3 cut(s) 187, 190, 687
Bme1390I CCNGG 1 cut(s) 670
Bme18I GGWCC 1 cut(s) 274
BmeRI GACNNNNNGTC 1 cut(s) 618
BmeT110I CYCGRG 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 620
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 2 cut(s) 431, 451
BmrFI CCNGG 1 cut(s) 670
BmsI GCATC 4 cut(s) 134, 295, 397, 502
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BsaJI CCNNGG 3 cut(s) 225, 510, 668
Bsc4I CCNNNNNNNGG 1 cut(s) 516
Bse1I ACTGG 4 cut(s) 155, 329, 436, 525
Bse3DI GCAATG 1 cut(s) 631
BseBI CCWGG 1 cut(s) 670
BseDI CCNNGG 3 cut(s) 225, 510, 668
BseGI GGATG 4 cut(s) 79, 319, 510, 722
BseLI CCNNNNNNNGG 1 cut(s) 516
BseMI GCAATG 1 cut(s) 631
BseMII CTCAG 2 cut(s) 143, 750
BseNI ACTGG 4 cut(s) 155, 329, 436, 525
BseRI GAGGAG 2 cut(s) 213, 264
BseSI GKGCMC 1 cut(s) 454
BseXI GCAGC 3 cut(s) 197, 200, 697
BseYI CCCAGC 1 cut(s) 596
BsgI GTGCAG 1 cut(s) 704
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 2 cut(s) 98, 549
BshNI GGYRCC 2 cut(s) 429, 449
BsiEI CGRYCG 1 cut(s) 367
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 1 cut(s) 573
BslI CCNNNNNNNGG 1 cut(s) 516
BsmAI GTCTC 1 cut(s) 374
BsnI GGCC 2 cut(s) 98, 549
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 454
Bsp143I GATC 5 cut(s) 165, 214, 471, 556, 664
BspACI CCGC 2 cut(s) 410, 454
BspANI GGCC 2 cut(s) 98, 549
BspCNI CTCAG 2 cut(s) 142, 751
BspLI GGNNCC 2 cut(s) 431, 451
BspMI ACCTGC 1 cut(s) 658
BspPI GGATC 3 cut(s) 479, 551, 659
BspT107I GGYRCC 2 cut(s) 429, 449
BsrDI GCAATG 1 cut(s) 631
BsrI ACTGG 4 cut(s) 155, 329, 436, 525
BssECI CCNNGG 3 cut(s) 225, 510, 668
BssMI GATC 5 cut(s) 165, 214, 471, 556, 664
BssT1I CCWWGG 1 cut(s) 510
Bst2UI CCWGG 1 cut(s) 670
Bst4CI ACNGT 2 cut(s) 176, 365
BstC8I GCNNGC 3 cut(s) 336, 454, 551
BstDEI CTNAG 3 cut(s) 129, 278, 759
BstENI CCTNNNNNAGG 1 cut(s) 514
BstF5I GGATG 4 cut(s) 79, 319, 510, 722
BstKTI GATC 5 cut(s) 168, 217, 474, 559, 667
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 5 cut(s) 165, 214, 471, 556, 664
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 1 cut(s) 291
BstNI CCWGG 1 cut(s) 670
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 668
BstSLI GKGCMC 1 cut(s) 454
BstV1I GCAGC 3 cut(s) 197, 200, 697
BsuRI GGCC 2 cut(s) 98, 549
BtrI CACGTC 1 cut(s) 620
BtsCI GGATG 4 cut(s) 79, 319, 510, 722
BtsI GCAGTG 2 cut(s) 315, 615
BtsIMutI CAGTG 2 cut(s) 315, 615
BveI ACCTGC 1 cut(s) 658
Cac8I GCNNGC 3 cut(s) 336, 454, 551
Cfr13I GGNCC 1 cut(s) 274
Csp6I GTAC 1 cut(s) 342
CviAII CATG 2 cut(s) 34, 339
CviJI RGCY 4 cut(s) 91, 98, 230, 549
CviKI_1 RGCY 4 cut(s) 91, 98, 230, 549
CviQI GTAC 1 cut(s) 342
DdeI CTNAG 3 cut(s) 129, 278, 759
DpnI GATC 5 cut(s) 167, 216, 473, 558, 666
DpnII GATC 5 cut(s) 165, 214, 471, 556, 664
DriI GACNNNNNGTC 1 cut(s) 618
EaeI YGGCCR 1 cut(s) 547
Eam1105I GACNNNNNGTC 1 cut(s) 618
Eco130I CCWWGG 1 cut(s) 510
Eco147I AGGCCT 1 cut(s) 98
Eco32I GATATC 1 cut(s) 208
Eco47I GGWCC 1 cut(s) 274
Eco88I CYCGRG 1 cut(s) 86
EcoNI CCTNNNNNAGG 1 cut(s) 514
EcoRII CCWGG 1 cut(s) 668
EcoRV GATATC 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 2 cut(s) 37, 342
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FatI CATG 2 cut(s) 33, 338
FauI CCCGC 1 cut(s) 461
Fnu4HI GCNGC 3 cut(s) 186, 189, 686
FokI GGATG 4 cut(s) 86, 326, 517, 729
Fsp4HI GCNGC 3 cut(s) 186, 189, 686
FspBI CTAG 1 cut(s) 459
GluI GCNGC 3 cut(s) 186, 189, 686
GsaI CCCAGC 1 cut(s) 600
HaeIII GGCC 2 cut(s) 98, 549
HapII CCGG 1 cut(s) 573
Hin1II CATG 2 cut(s) 37, 342
HinfI GANTC 2 cut(s) 413, 721
HpaII CCGG 1 cut(s) 573
Hpy188I TCNGA 3 cut(s) 165, 301, 760
Hpy188III TCNNGA 1 cut(s) 368
Hpy99I CGWCG 1 cut(s) 249
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 2 cut(s) 176, 365
HpyCH4IV ACGT 1 cut(s) 619
HpyCH4V TGCA 6 cut(s) 125, 171, 185, 308, 653, 685
HpyF10VI GCNNNNNNNGC 1 cut(s) 291
HpyF3I CTNAG 3 cut(s) 129, 278, 759
HpySE526I ACGT 1 cut(s) 619
Hsp92II CATG 2 cut(s) 37, 342
Kzo9I GATC 5 cut(s) 165, 214, 471, 556, 664
Lsp1109I GCAGC 3 cut(s) 197, 200, 697
LweI GCATC 4 cut(s) 134, 295, 397, 502
MaeI CTAG 1 cut(s) 459
MaeII ACGT 1 cut(s) 619
MaeIII GTNAC 2 cut(s) 527, 610
MalI GATC 5 cut(s) 167, 216, 473, 558, 666
MboI GATC 5 cut(s) 165, 214, 471, 556, 664
MboII GAAGA 2 cut(s) 529, 747
MhlI GDGCHC 1 cut(s) 454
MlsI TGGCCA 1 cut(s) 549
MluCI AATT 4 cut(s) 57, 219, 235, 348
MluNI TGGCCA 1 cut(s) 549
MmeI TCCRAC 1 cut(s) 170
MnlI CCTC 7 cut(s) 64, 153, 191, 235, 242, 254, 510
Mox20I TGGCCA 1 cut(s) 549
MscI TGGCCA 1 cut(s) 549
MseI TTAA 1 cut(s) 234
Msp20I TGGCCA 1 cut(s) 549
MspI CCGG 1 cut(s) 573
MspR9I CCNGG 1 cut(s) 670
MvaI CCWGG 1 cut(s) 670
MwoI GCNNNNNNNGC 1 cut(s) 291
NdeII GATC 5 cut(s) 165, 214, 471, 556, 664
NlaIII CATG 2 cut(s) 37, 342
NlaIV GGNNCC 2 cut(s) 431, 451
NmeAIII GCCGAG 1 cut(s) 206
NmuCI GTSAC 1 cut(s) 610
NspI RCATGY 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 86
PceI AGGCCT 1 cut(s) 98
PfeI GAWTC 2 cut(s) 413, 721
PkrI GCNGC 3 cut(s) 187, 190, 687
Psp6I CCWGG 1 cut(s) 668
PspFI CCCAGC 1 cut(s) 596
PspGI CCWGG 1 cut(s) 668
PspN4I GGNNCC 2 cut(s) 431, 451
PspPI GGNCC 1 cut(s) 274
PspXI VCTCGAGB 1 cut(s) 86
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 3 cut(s) 186, 189, 686
Sau3AI GATC 5 cut(s) 165, 214, 471, 556, 664
Sau96I GGNCC 1 cut(s) 274
ScrFI CCNGG 1 cut(s) 670
SduI GDGCHC 1 cut(s) 454
SfaNI GCATC 4 cut(s) 134, 295, 397, 502
Sfr274I CTCGAG 1 cut(s) 86
SinI GGWCC 1 cut(s) 274
SlaI CTCGAG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 4 cut(s) 57, 219, 235, 348
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 2 cut(s) 410, 454
SspMI CTAG 1 cut(s) 459
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 668
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 2 cut(s) 176, 365
TaiI ACGT 1 cut(s) 622
TaqI TCGA 4 cut(s) 87, 217, 247, 367
TasI AATT 4 cut(s) 57, 219, 235, 348
TatI WGTACW 1 cut(s) 341
TfiI GAWTC 2 cut(s) 413, 721
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 2 cut(s) 315, 615
TseFI GTSAC 1 cut(s) 610
TseI GCWGC 3 cut(s) 185, 188, 685
Tsp45I GTSAC 1 cut(s) 610
TspDTI ATGAA 3 cut(s) 22, 96, 100
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 2 cut(s) 315, 615
VpaK11BI GGWCC 1 cut(s) 274
XagI CCTNNNNNAGG 1 cut(s) 514
XapI RAATTY 1 cut(s) 348
XceI RCATGY 1 cut(s) 342
XhoI CTCGAG 1 cut(s) 86
XspI CTAG 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.