RchiOBHm_Chr4g0406651

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0013958
Physical Location & Seq
Reverse (-)
28428384 .. 28429635
1252 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 453 bp
ATGGAGCCGACTGAGCTGAAGCGCGTGTTCCAAATGTTCGACCAGAACGGCGACGGGCGCATCAGCAGAAAGGAGCTGAGCGATTCGCTGGAGAACTTGGGCATCTTCATCCCTGACAAGGAGCTGTTCAACATGATCCAGAAGATCGACGTCGACGGAGACGGGTGCGTCGACATTGACGAGTTCGGCGAGCTGTACCAATCCATAATGGACGAGCGGAACGAGGAGGAGGACATGAAGGAGGCATTCAACGTTTTCGATCAGAACGGTGATGGCTTCATCGCCGTCGACGAGTTGAGGTCCGTCTTGTCCTCCCTCGGCCTCAAGCAAGGCAAGTCCATAGAGGACTGCAAGAGGATGATTATGAATGTCGACGTCGATGGAGATGGCAGGGTCAATTACAAGGAGTTCAAGCAGATGATGAAGGGAGGAGGCTTTAGCGCTTTAACATAG

Protein Analysis

150

Amino Acids

17.04

Weight (kDa)

4.32

Isoelectric Point (pI)

31.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 4 - 67 9.9e-15 EF-hand domain pair
EF-hand_1 PF00036 5 - 30 5.3e-07 EF hand domain
EF-hand_6 PF13405 5 - 34 1.3e-07 EF-hand domain
EF-hand_5 PF13202 6 - 27 5.4e-07 EF hand
EF-hand_1 PF00036 41 - 67 3.3e-06 EF hand domain
AIF-1 PF21008 67 - 144 3.2e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 76 - 142 3.6e-16 EF-hand domain pair
EF-hand_6 PF13405 78 - 107 1.8e-08 EF-hand domain
EF-hand_1 PF00036 79 - 106 4.4e-07 EF hand domain
EF-hand_5 PF13202 80 - 102 8.8e-06 EF hand
EF-hand_1 PF00036 116 - 142 6e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43290
fragaria_vesca FvH4_4g10830
malus_domestica MD04G1041400.v1.1 MD13G1272800.v1.1
prunus_persica Prupe.1G194800_v2.0.a1
pyrus_communis pycom04g03640
rosa_chinensis RchiOBHm_Chr4g0406651
rosa_laevigata RLG00000008762
rosa_multiflora Rmu_co8120260.1_g000001 Rmu_sc0002774.1_g000003 Rmu_sc0019587.1_g000005
rosa_roxburghii Rroxscaffold_5G00351490
rosa_rugosa Rorug04G0072700
rosa_samantha Rh4AG140000 Rh4BG136900 Rh4DG134100
rosa_wichuraiana Rw4G011400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AatII GACGTC 2 cut(s) 153, 378
AccBSI CCGCTC 1 cut(s) 217
AccI GTMKAC 4 cut(s) 153, 171, 288, 372
AccII CGCG 1 cut(s) 24
AciI CCGC 1 cut(s) 217
AclI AACGTT 1 cut(s) 252
AclWI GGATC 1 cut(s) 130
AcuI CTGAAG 1 cut(s) 38
AcyI GRCGYC 2 cut(s) 150, 375
AfaI GTAC 1 cut(s) 197
AfeI AGCGCT 1 cut(s) 442
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 3 cut(s) 130, 250, 412
AluBI AGCT 4 cut(s) 16, 76, 124, 193
AluI AGCT 4 cut(s) 16, 76, 124, 193
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 1 cut(s) 130
Aor51HI AGCGCT 1 cut(s) 442
AoxI GGCC 1 cut(s) 319
AspLEI GCGC 3 cut(s) 24, 60, 443
AspS9I GGNCC 1 cut(s) 300
AsuHPI GGTGA 1 cut(s) 281
AvaII GGWCC 1 cut(s) 300
BccI CCATC 3 cut(s) 266, 374, 380
BceAI ACGGC 2 cut(s) 64, 269
BcoDI GTCTC 1 cut(s) 153
BfoI RGCGCY 1 cut(s) 444
BlpI GCTNAGC 1 cut(s) 77
Bme18I GGWCC 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 300
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 2 cut(s) 69, 111
BpmI CTGGAG 1 cut(s) 110
Bpu1102I GCTNAGC 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 308
BsaHI GRCGYC 2 cut(s) 150, 375
BsaJI CCNNGG 1 cut(s) 316
Bsc4I CCNNNNNNNGG 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 2 cut(s) 108, 363
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 68
BseRI GAGGAG 3 cut(s) 239, 242, 444
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 1 cut(s) 153
BsmBI CGTCTC 1 cut(s) 153
BsmI GAATGC 1 cut(s) 245
BsnI GGCC 1 cut(s) 321
Bsp143I GATC 3 cut(s) 135, 144, 259
Bsp1720I GCTNAGC 1 cut(s) 77
BspACI CCGC 1 cut(s) 217
BspANI GGCC 1 cut(s) 321
BspCNI CTCAG 2 cut(s) 4, 69
BspFNI CGCG 1 cut(s) 24
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 130
BsrBI CCGCTC 1 cut(s) 217
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 3 cut(s) 135, 144, 259
BssNI GRCGYC 2 cut(s) 150, 375
Bst4CI ACNGT 1 cut(s) 269
BstACI GRCGYC 2 cut(s) 150, 375
BstC8I GCNNGC 1 cut(s) 191
BstDEI CTNAG 2 cut(s) 12, 77
BstF5I GGATG 2 cut(s) 108, 363
BstFNI CGCG 1 cut(s) 24
BstH2I RGCGCY 1 cut(s) 444
BstHHI GCGC 3 cut(s) 24, 60, 443
BstKTI GATC 3 cut(s) 138, 147, 262
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 3 cut(s) 135, 144, 259
BstMWI GCNNNNNNNGC 2 cut(s) 13, 57
BstUI CGCG 1 cut(s) 24
BsuRI GGCC 1 cut(s) 321
BtgZI GCGATG 1 cut(s) 265
BtsCI GGATG 2 cut(s) 108, 363
Cac8I GCNNGC 1 cut(s) 191
CfoI GCGC 3 cut(s) 24, 60, 443
Cfr13I GGNCC 1 cut(s) 300
CseI GACGC 1 cut(s) 157
Csp6I GTAC 1 cut(s) 196
CviAII CATG 2 cut(s) 133, 235
CviJI RGCY 8 cut(s) 7, 16, 76, 124, 193, 276, 321, 435
CviKI_1 RGCY 8 cut(s) 7, 16, 76, 124, 193, 276, 321, 435
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 2 cut(s) 12, 77
DpnI GATC 3 cut(s) 137, 146, 261
DpnII GATC 3 cut(s) 135, 144, 259
DrdI GACNNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 167
Eco47I GGWCC 1 cut(s) 300
Eco47III AGCGCT 1 cut(s) 442
Eco57I CTGAAG 1 cut(s) 38
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 2 cut(s) 136, 238
FaiI YATR 6 cut(s) 134, 206, 236, 341, 365, 451
FatI CATG 2 cut(s) 132, 234
FblI GTMKAC 4 cut(s) 153, 171, 288, 372
FokI GGATG 2 cut(s) 95, 370
GlaI GCGC 3 cut(s) 23, 59, 442
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 444
HaeIII GGCC 1 cut(s) 321
HgaI GACGC 1 cut(s) 157
HhaI GCGC 3 cut(s) 24, 60, 443
Hin1I GRCGYC 2 cut(s) 150, 375
Hin1II CATG 2 cut(s) 136, 238
Hin6I GCGC 3 cut(s) 22, 58, 441
HinP1I GCGC 3 cut(s) 22, 58, 441
HincII GTYRAC 4 cut(s) 154, 172, 289, 373
HindII GTYRAC 4 cut(s) 154, 172, 289, 373
HinfI GANTC 1 cut(s) 83
HphI GGTGA 1 cut(s) 281
Hpy166II GTNNAC 4 cut(s) 154, 172, 289, 373
Hpy188I TCNGA 1 cut(s) 264
Hpy188III TCNNGA 1 cut(s) 139
Hpy8I GTNNAC 4 cut(s) 154, 172, 289, 373
Hpy99I CGWCG 9 cut(s) 56, 152, 155, 158, 173, 290, 293, 377, 380
HpyAV CCTTC 2 cut(s) 232, 418
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 3 cut(s) 150, 252, 375
HpyCH4V TGCA 1 cut(s) 351
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 57
HpyF3I CTNAG 2 cut(s) 12, 77
HpySE526I ACGT 3 cut(s) 150, 252, 375
Hsp92I GRCGYC 2 cut(s) 150, 375
Hsp92II CATG 2 cut(s) 136, 238
HspAI GCGC 3 cut(s) 22, 58, 441
Kzo9I GATC 3 cut(s) 135, 144, 259
LmnI GCTCC 3 cut(s) 4, 73, 121
LpnPI CCDG 5 cut(s) 56, 74, 126, 152, 376
LweI GCATC 2 cut(s) 69, 111
MaeII ACGT 3 cut(s) 150, 252, 375
MalI GATC 3 cut(s) 137, 146, 261
MbiI CCGCTC 1 cut(s) 217
MboI GATC 3 cut(s) 135, 144, 259
MboII GAAGA 2 cut(s) 97, 154
MluCI AATT 1 cut(s) 397
MseI TTAA 1 cut(s) 446
Mva1269I GAATGC 1 cut(s) 245
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 2 cut(s) 13, 57
NdeII GATC 3 cut(s) 135, 144, 259
NlaIII CATG 2 cut(s) 136, 238
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 297
PcsI WCGNNNNNNNCGW 5 cut(s) 168, 177, 186, 219, 288
PctI GAATGC 1 cut(s) 245
PfeI GAWTC 1 cut(s) 83
Psp1406I AACGTT 1 cut(s) 252
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 300
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SalI GTCGAC 4 cut(s) 152, 170, 287, 371
SaqAI TTAA 1 cut(s) 446
Sau3AI GATC 3 cut(s) 135, 144, 259
Sau96I GGNCC 1 cut(s) 300
SetI ASST 8 cut(s) 18, 78, 126, 153, 195, 255, 302, 378
SfaNI GCATC 2 cut(s) 69, 111
SgrDI CGTCGACG 2 cut(s) 152, 287
SinI GGWCC 1 cut(s) 300
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 1 cut(s) 397
SsiI CCGC 1 cut(s) 217
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 3 cut(s) 153, 255, 378
TaqI TCGA 8 cut(s) 39, 147, 153, 171, 258, 288, 372, 378
TasI AATT 1 cut(s) 397
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TspDTI ATGAA 5 cut(s) 97, 251, 268, 380, 437
TspGWI ACGGA 2 cut(s) 171, 292
VpaK11BI GGWCC 1 cut(s) 300
XmiI GTMKAC 4 cut(s) 153, 171, 288, 372
ZraI GACGTC 2 cut(s) 151, 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.