RchiOBHm_Chr4g0440591

DUF1338

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010892
Physical Location & Seq
Forward (+)
62688768 .. 62691445
2678 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1293 bp
ATGTGGCGGCCAGACAGAACAGGAAGCAAGCAAACGCCACTAAACACATCGAAGAAGTTCAGCCAAAAAAAAAACACATCGAAGAAGAGAAAGAAGATCAAAATATTCCATACCACACAGGATTGTTTCCATTCCAAGGTTGAAGAAGACATGATTACGCGATTCTCCACCACCATGACCATGTTAAGAAGCTCCTCTATCTCCTCCCCATCACTTGACTCTCTCATTCCTCGCCAACCCAACAGTGTCAATCCCACCAGGAATCTCATCTCCCTCACCAAATCACCTCTTCCCCTGAATCTCAGATACTCAATCTCCATGAACAATCAAAATCTCGGCACCGTTTGTAGCTCCAAATCAGACAATGGATCCCATGACCCTTCTTCTTCTCAGGGAGGTGAATCATTCTTCAGGGGAGTGTTGGCAAACATGGAGGCTGTGTACTTGAGCAGGAACCCTACAGCAAAGTCAGTGTTGGAGCTTGTTGGGTCTGATGAACAGATTAGCTATGATCATTTTGCATTCAGGACCTTTGGGGTGAATGGTTATGGGATTGATTCCATGGCTAGATTTTTCTTGGATTTCGGGTACACTCCCCGGGAGGAGTTACGATTCCCGGCTAAGAAATTGAGGGCATTCTGGTTTGCTCCTCCTAGTTATACCATTTCTGATGGTGGTGCTGGTGTTAATGGGCCTTTGCCGAGAATATTTATATCCGAGCTTCTTGTAGATCAGATGAGTCCACAAACTCAGGAGATAATCCGTAAGTACACTGAAAACTCTGGTTCTGGAAATAAGTATGCTGCTCTAGCAAGTGCGCTGGGAACTTTGACGTGGGAAAAGCCGTTGTACTCAGAATTTCAGCAATTGGCTAGGGAAAGTGAATATGCTGCCTGGACCCTTGTCAATGGGTATGCAGTCAACCATGTCACAATCTCTGCTCACCAGCTGAAATCTAAATTGAGAGATATTAAAAGCCTCAATCAATTCATCGAGGAGAATGGGTTCAGGTTGAATTCTGAAGGCGGAATTCTGAAAGTGAGCCCCGATGGTCTTCTGCTGCAAAGTTCAACTGTCGCAGATCCAGTTAATTTCCAATTTAGGGATGGTCAAATTGAGTCGGTCCCCTGCTCCTACATTGAGTTTGCTCAACGCCTGGTGCTGCCCCAGTACAGCAATCTACCAAGCAACGAGGTTAAAGAGCATCACAGGCGAGATGGCTTTGAGGTTGGTAACGCGGACAAGATCTTTGAGAGCACATCAAAGGAGCAGCTGACCAGGAGGGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1338

Protein Analysis

430

Amino Acids

48.23

Weight (kDa)

9.26

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGLS PF07063 157 - 388 1.2e-42 2-oxoadipate dioxygenase/decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 338
AccII CGCG 2 cut(s) 160, 1238
AciI CCGC 3 cut(s) 7, 1026, 1238
AclWI GGATC 3 cut(s) 363, 376, 1076
AcoI YGGCCR 1 cut(s) 8
AcsI RAATTY 3 cut(s) 857, 1015, 1029
AcuI CTGAAG 2 cut(s) 394, 1041
AfaI GTAC 5 cut(s) 443, 590, 770, 851, 1172
AfiI CCNNNNNNNGG 2 cut(s) 136, 1102
AgsI TTSAA 3 cut(s) 143, 1015, 1071
AjiI CACGTC 1 cut(s) 834
AjnI CCWGG 4 cut(s) 257, 893, 1155, 1277
AloI GAACNNNNNNTCC 2 cut(s) 989, 1021
AluBI AGCT 7 cut(s) 192, 351, 481, 507, 721, 949, 1273
AluI AGCT 7 cut(s) 192, 351, 481, 507, 721, 949, 1273
Alw21I GWGCWC 1 cut(s) 1259
AlwI GGATC 3 cut(s) 363, 376, 1076
Ama87I CYCGRG 1 cut(s) 597
AoxI GGCC 2 cut(s) 8, 692
ApeKI GCWGC 6 cut(s) 803, 890, 1060, 1162, 1270, 1286
ApoI RAATTY 3 cut(s) 857, 1015, 1029
ArsI GACNNNNNNTTYG 2 cut(s) 1232, 1264
Asp700I GAANNNNTTC 2 cut(s) 56, 1004
AspLEI GCGC 1 cut(s) 820
AspS9I GGNCC 4 cut(s) 528, 692, 897, 1123
AsuC2I CCSGG 3 cut(s) 598, 599, 617
AsuHPI GGTGA 5 cut(s) 268, 276, 410, 550, 935
AvaI CYCGRG 1 cut(s) 597
AvaII GGWCC 3 cut(s) 528, 897, 1123
BamHI GGATCC 1 cut(s) 368
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 1046
BbsI GAAGAC 2 cut(s) 153, 1046
Bbv12I GWGCWC 1 cut(s) 1259
BbvI GCAGC 6 cut(s) 790, 877, 1047, 1149, 1273, 1282
BccI CCATC 5 cut(s) 217, 665, 1043, 1100, 1211
BceAI ACGGC 1 cut(s) 829
BciT130I CCWGG 4 cut(s) 259, 895, 1157, 1279
BclI TGATCA 1 cut(s) 511
BcnI CCSGG 3 cut(s) 598, 599, 617
BfaI CTAG 4 cut(s) 567, 654, 809, 873
BfmI CTRYAG 1 cut(s) 459
BglII AGATCT 1 cut(s) 1245
BisI GCNGC 7 cut(s) 8, 804, 891, 1061, 1163, 1271, 1287
BlsI GCNGC 7 cut(s) 9, 805, 892, 1062, 1164, 1272, 1288
Bme1390I CCNGG 7 cut(s) 259, 598, 599, 617, 895, 1157, 1279
Bme18I GGWCC 3 cut(s) 528, 897, 1123
BmeT110I CYCGRG 1 cut(s) 597
BmgBI CACGTC 1 cut(s) 834
BmgT120I GGNCC 4 cut(s) 528, 692, 897, 1123
BmiI GGNNCC 5 cut(s) 340, 370, 455, 899, 1125
BmrFI CCNGG 7 cut(s) 259, 598, 599, 617, 895, 1157, 1279
BmrI ACTGGG 1 cut(s) 1162
BmsI GCATC 1 cut(s) 1213
BmuI ACTGGG 1 cut(s) 1162
BoxI GACNNNNGTC 1 cut(s) 902
BpiI GAAGAC 2 cut(s) 153, 1046
BpuEI CTTGAG 1 cut(s) 466
BpuMI CCSGG 3 cut(s) 598, 599, 617
BsaJI CCNNGG 4 cut(s) 135, 561, 596, 597
BsaXI ACNNNNNCTCC 6 cut(s) 299, 329, 425, 455, 989, 1019
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 1102
Bse1I ACTGG 2 cut(s) 1085, 1168
BseBI CCWGG 4 cut(s) 259, 895, 1157, 1279
BseDI CCNNGG 4 cut(s) 135, 561, 596, 597
BseGI GGATG 1 cut(s) 1111
BseLI CCNNNNNNNGG 2 cut(s) 136, 1102
BseMII CTCAG 4 cut(s) 316, 404, 764, 867
BseNI ACTGG 2 cut(s) 1085, 1168
BseRI GAGGAG 5 cut(s) 184, 193, 617, 639, 1010
BseXI GCAGC 6 cut(s) 790, 877, 1047, 1149, 1273, 1282
BseYI CCCAGC 1 cut(s) 820
Bsh1236I CGCG 2 cut(s) 160, 1238
BshFI GGCC 2 cut(s) 10, 694
BshNI GGYRCC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 1259
BsiHKCI CYCGRG 1 cut(s) 597
BsiSI CCGG 2 cut(s) 598, 617
BslFI GGGAC 1 cut(s) 1109
BslI CCNNNNNNNGG 2 cut(s) 136, 1102
BsmFI GGGAC 1 cut(s) 1109
BsmI GAATGC 2 cut(s) 521, 635
BsnI GGCC 2 cut(s) 10, 694
BsoBI CYCGRG 1 cut(s) 597
Bsp1286I GDGCHC 2 cut(s) 1046, 1259
Bsp143I GATC 6 cut(s) 96, 368, 511, 730, 1081, 1245
Bsp19I CCATGG 1 cut(s) 561
BspACI CCGC 3 cut(s) 7, 1026, 1238
BspANI GGCC 2 cut(s) 10, 694
BspCNI CTCAG 4 cut(s) 315, 403, 763, 866
BspFNI CGCG 2 cut(s) 160, 1238
BspLI GGNNCC 5 cut(s) 340, 370, 455, 899, 1125
BspPI GGATC 3 cut(s) 363, 376, 1076
BspT107I GGYRCC 1 cut(s) 338
BsrI ACTGG 2 cut(s) 1085, 1168
BssECI CCNNGG 4 cut(s) 135, 561, 596, 597
BssMI GATC 6 cut(s) 96, 368, 511, 730, 1081, 1245
BssT1I CCWWGG 2 cut(s) 135, 561
Bst2UI CCWGG 4 cut(s) 259, 895, 1157, 1279
Bst4CI ACNGT 3 cut(s) 245, 343, 1075
Bst6I CTCTTC 2 cut(s) 80, 294
BstC8I GCNNGC 1 cut(s) 29
BstDEI CTNAG 5 cut(s) 302, 390, 621, 750, 853
BstDSI CCRYGG 1 cut(s) 561
BstF5I GGATG 1 cut(s) 1111
BstFNI CGCG 2 cut(s) 160, 1238
BstHHI GCGC 1 cut(s) 820
BstKTI GATC 6 cut(s) 99, 371, 514, 733, 1084, 1248
BstMBI GATC 6 cut(s) 96, 368, 511, 730, 1081, 1245
BstMWI GCNNNNNNNGC 2 cut(s) 809, 1210
BstNI CCWGG 4 cut(s) 259, 895, 1157, 1279
BstPAI GACNNNNGTC 1 cut(s) 902
BstSCI CCNGG 7 cut(s) 257, 596, 597, 615, 893, 1155, 1277
BstSFI CTRYAG 1 cut(s) 459
BstUI CGCG 2 cut(s) 160, 1238
BstV1I GCAGC 6 cut(s) 790, 877, 1047, 1149, 1273, 1282
BstV2I GAAGAC 2 cut(s) 153, 1046
BstX2I RGATCY 3 cut(s) 368, 1081, 1245
BstYI RGATCY 3 cut(s) 368, 1081, 1245
BsuRI GGCC 2 cut(s) 10, 694
BtgI CCRYGG 1 cut(s) 561
BtrI CACGTC 1 cut(s) 834
BtsCI GGATG 1 cut(s) 1111
BtsIMutI CAGTG 3 cut(s) 250, 477, 771
Cac8I GCNNGC 1 cut(s) 29
CfoI GCGC 1 cut(s) 820
Cfr13I GGNCC 4 cut(s) 528, 692, 897, 1123
Cfr9I CCCGGG 1 cut(s) 597
Csp6I GTAC 5 cut(s) 442, 589, 769, 850, 1171
CviAII CATG 9 cut(s) 151, 175, 181, 319, 374, 430, 562, 926, 1290
CviQI GTAC 5 cut(s) 442, 589, 769, 850, 1171
DdeI CTNAG 5 cut(s) 302, 390, 621, 750, 853
DpnI GATC 6 cut(s) 98, 370, 513, 732, 1083, 1247
DpnII GATC 6 cut(s) 96, 368, 511, 730, 1081, 1245
EaeI YGGCCR 1 cut(s) 8
Eam1104I CTCTTC 2 cut(s) 80, 294
EarI CTCTTC 2 cut(s) 80, 294
EciI GGCGGA 1 cut(s) 1041
Eco130I CCWWGG 2 cut(s) 135, 561
Eco24I GRGCYC 1 cut(s) 1046
Eco47I GGWCC 3 cut(s) 528, 897, 1123
Eco57I CTGAAG 2 cut(s) 394, 1041
Eco88I CYCGRG 1 cut(s) 597
EcoO109I RGGNCCY 1 cut(s) 528
EcoRI GAATTC 2 cut(s) 1015, 1029
EcoRII CCWGG 4 cut(s) 257, 893, 1155, 1277
EcoT14I CCWWGG 2 cut(s) 135, 561
EcoT38I GRGCYC 1 cut(s) 1046
ErhI CCWWGG 2 cut(s) 135, 561
FaeI CATG 9 cut(s) 154, 178, 184, 322, 377, 433, 565, 929, 1293
FaqI GGGAC 1 cut(s) 1109
FatI CATG 9 cut(s) 150, 174, 180, 318, 373, 429, 561, 925, 1289
FbaI TGATCA 1 cut(s) 511
Fnu4HI GCNGC 7 cut(s) 8, 804, 891, 1061, 1163, 1271, 1287
FokI GGATG 1 cut(s) 1118
FriOI GRGCYC 1 cut(s) 1046
Fsp4HI GCNGC 7 cut(s) 8, 804, 891, 1061, 1163, 1271, 1287
FspBI CTAG 4 cut(s) 567, 654, 809, 873
GlaI GCGC 1 cut(s) 819
GluI GCNGC 7 cut(s) 8, 804, 891, 1061, 1163, 1271, 1287
GsaI CCCAGC 1 cut(s) 824
HaeIII GGCC 2 cut(s) 10, 694
HapII CCGG 2 cut(s) 598, 617
HhaI GCGC 1 cut(s) 820
Hin1II CATG 9 cut(s) 154, 178, 184, 322, 377, 433, 565, 929, 1293
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HinfI GANTC 9 cut(s) 162, 218, 262, 298, 401, 557, 612, 739, 1118
HpaII CCGG 2 cut(s) 598, 617
HphI GGTGA 5 cut(s) 268, 276, 410, 550, 935
Hpy166II GTNNAC 5 cut(s) 442, 591, 743, 771, 922
Hpy188I TCNGA 9 cut(s) 305, 361, 493, 670, 718, 735, 856, 1021, 1035
Hpy188III TCNNGA 3 cut(s) 526, 752, 789
Hpy8I GTNNAC 5 cut(s) 442, 591, 743, 771, 922
HpyAV CCTTC 2 cut(s) 390, 1016
HpyCH4III ACNGT 3 cut(s) 245, 343, 1075
HpyCH4IV ACGT 1 cut(s) 833
HpyCH4V TGCA 4 cut(s) 521, 917, 1063, 1289
HpyF10VI GCNNNNNNNGC 2 cut(s) 809, 1210
HpyF3I CTNAG 5 cut(s) 302, 390, 621, 750, 853
HpySE526I ACGT 1 cut(s) 833
Hsp92II CATG 9 cut(s) 154, 178, 184, 322, 377, 433, 565, 929, 1293
HspAI GCGC 1 cut(s) 818
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 6 cut(s) 96, 368, 511, 730, 1081, 1245
LmnI GCTCC 6 cut(s) 197, 356, 478, 652, 1136, 1267
Lsp1109I GCAGC 6 cut(s) 790, 877, 1047, 1149, 1273, 1282
LweI GCATC 1 cut(s) 1213
MaeI CTAG 4 cut(s) 567, 654, 809, 873
MaeII ACGT 1 cut(s) 833
MaeIII GTNAC 3 cut(s) 606, 928, 1232
MalI GATC 6 cut(s) 98, 370, 513, 732, 1083, 1247
MboI GATC 6 cut(s) 96, 368, 511, 730, 1081, 1245
MfeI CAATTG 1 cut(s) 866
MflI RGATCY 3 cut(s) 368, 1081, 1245
MhlI GDGCHC 2 cut(s) 1046, 1259
MlyI GAGTC 3 cut(s) 212, 748, 1127
MmeI TCCRAC 1 cut(s) 456
MroXI GAANNNNTTC 2 cut(s) 56, 1004
MseI TTAA 5 cut(s) 185, 687, 972, 1089, 1197
MslI CAYNNNNRTG 2 cut(s) 173, 179
MspA1I CMGCKG 2 cut(s) 949, 1273
MspI CCGG 2 cut(s) 598, 617
MspR9I CCNGG 7 cut(s) 259, 598, 599, 617, 895, 1157, 1279
MunI CAATTG 1 cut(s) 866
Mva1269I GAATGC 2 cut(s) 521, 635
MvaI CCWGG 4 cut(s) 259, 895, 1157, 1279
MvnI CGCG 2 cut(s) 160, 1238
MwoI GCNNNNNNNGC 2 cut(s) 809, 1210
NciI CCSGG 3 cut(s) 598, 599, 617
NcoI CCATGG 1 cut(s) 561
NdeII GATC 6 cut(s) 96, 368, 511, 730, 1081, 1245
NlaIII CATG 9 cut(s) 154, 178, 184, 322, 377, 433, 565, 929, 1293
NlaIV GGNNCC 5 cut(s) 340, 370, 455, 899, 1125
NmeAIII GCCGAG 2 cut(s) 315, 726
NmuCI GTSAC 1 cut(s) 928
PctI GAATGC 2 cut(s) 521, 635
PdmI GAANNNNTTC 2 cut(s) 56, 1004
PfeI GAWTC 6 cut(s) 162, 262, 298, 401, 557, 612
PkrI GCNGC 7 cut(s) 9, 805, 892, 1062, 1164, 1272, 1288
PleI GAGTC 3 cut(s) 212, 747, 1126
PpsI GAGTC 3 cut(s) 212, 747, 1126
PpuMI RGGWCCY 1 cut(s) 528
PshAI GACNNNNGTC 1 cut(s) 902
Psp5II RGGWCCY 1 cut(s) 528
Psp6I CCWGG 4 cut(s) 257, 893, 1155, 1277
PspFI CCCAGC 1 cut(s) 820
PspGI CCWGG 4 cut(s) 257, 893, 1155, 1277
PspN4I GGNNCC 5 cut(s) 340, 370, 455, 899, 1125
PspPI GGNCC 4 cut(s) 528, 692, 897, 1123
PspPPI RGGWCCY 1 cut(s) 528
PsuI RGATCY 3 cut(s) 368, 1081, 1245
PvuII CAGCTG 2 cut(s) 949, 1273
RsaI GTAC 5 cut(s) 443, 590, 770, 851, 1172
RsaNI GTAC 5 cut(s) 442, 589, 769, 850, 1171
RseI CAYNNNNRTG 2 cut(s) 173, 179
SaqAI TTAA 5 cut(s) 185, 687, 972, 1089, 1197
SatI GCNGC 7 cut(s) 8, 804, 891, 1061, 1163, 1271, 1287
Sau3AI GATC 6 cut(s) 96, 368, 511, 730, 1081, 1245
Sau96I GGNCC 4 cut(s) 528, 692, 897, 1123
SchI GAGTC 3 cut(s) 212, 748, 1127
ScrFI CCNGG 7 cut(s) 259, 598, 599, 617, 895, 1157, 1279
SduI GDGCHC 2 cut(s) 1046, 1259
SfaNI GCATC 1 cut(s) 1213
SfcI CTRYAG 1 cut(s) 459
SinI GGWCC 3 cut(s) 528, 897, 1123
SmaI CCCGGG 1 cut(s) 599
SmiMI CAYNNNNRTG 2 cut(s) 173, 179
SmlI CTYRAG 1 cut(s) 445
SmoI CTYRAG 1 cut(s) 445
SsiI CCGC 3 cut(s) 7, 1026, 1238
SspI AATATT 2 cut(s) 105, 708
SspMI CTAG 4 cut(s) 567, 654, 809, 873
StyD4I CCNGG 7 cut(s) 257, 596, 597, 615, 893, 1155, 1277
StyI CCWWGG 2 cut(s) 135, 561
TaaI ACNGT 3 cut(s) 245, 343, 1075
TaiI ACGT 1 cut(s) 836
TaqI TCGA 3 cut(s) 50, 80, 993
TaqII GACCGA 1 cut(s) 1111
TatI WGTACW 4 cut(s) 441, 768, 849, 1170
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 6 cut(s) 162, 262, 298, 401, 557, 612
Tru1I TTAA 5 cut(s) 185, 687, 972, 1089, 1197
Tru9I TTAA 5 cut(s) 185, 687, 972, 1089, 1197
TscAI CASTG 3 cut(s) 250, 477, 778
TseFI GTSAC 1 cut(s) 928
TseI GCWGC 6 cut(s) 803, 890, 1060, 1162, 1270, 1286
Tsp45I GTSAC 1 cut(s) 928
TspDTI ATGAA 3 cut(s) 335, 510, 979
TspGWI ACGGA 1 cut(s) 752
TspMI CCCGGG 1 cut(s) 597
TspRI CASTG 3 cut(s) 250, 477, 778
VpaK11BI GGWCC 3 cut(s) 528, 897, 1123
XapI RAATTY 3 cut(s) 857, 1015, 1029
XcmI CCANNNNNNNNNTGG 1 cut(s) 1103
XmaI CCCGGG 1 cut(s) 597
XmnI GAANNNNTTC 2 cut(s) 56, 1004
XspI CTAG 4 cut(s) 567, 654, 809, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.