RchiOBHm_Chr4g0443821

ER-associated misfolded protein catabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0017190
Physical Location & Seq
Forward (+)
64810211 .. 64810669
459 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 459 bp
ATGAGTGTCATTTTGCCTCAACCCCATATGATTTTCAGGTTCCCAAAGCCTGAAGACTACGACCACCTCGATGACGACGTCGTAGACGATCATCACCCTCCTCTCTCCGAAGAATCCGATGCATGTTTGGAAACTAGTCCCGATCACAATCCTCGTCATGAAAATGGTACTACCAGCAGCTCCAGACCTTCAACGGCGTCGTATTTCGATTGTAACTTGTGCTTCAAATCGGAGAGGCAGCCGGTTGTGACTGCGTGCGGGCATTTGTATTGCTACTCTTGCTTGTGCGACTGGCTGCAGTTTGGTCGGAAATGTCCTGTTTGTGAGGCCAAGGTGGATGACGGACGCATCTTTCCGATTTATCAATGCAGTGGACCGAGACCAAAGACACAATTGGTGCAAGTAAGTACCAGTACGAGTGAGCTAGAGAAAGATCGATTTTGTGGAGTATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4 zf-C3HC4_3

Protein Analysis

152

Amino Acids

17.23

Weight (kDa)

5.1

Isoelectric Point (pI)

57.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 70 - 113 1.6e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 70 - 108 7.7e-08 Ring finger domain
zf-C3HC4_2 PF13923 71 - 108 7.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 71 - 108 1.4e-07 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 71 - 117 5.7e-07 Prokaryotic RING finger family 4
zf-RING_5 PF14634 71 - 109 1.4e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74990
fragaria_vesca FvH4_4g34391
malus_domestica MD02G1215900.v1.1
prunus_persica Prupe.8G036400_v2.0.a1
pyrus_communis pycom07g09040
rosa_chinensis RchiOBHm_Chr4g0443821
rosa_laevigata RLG00000005885
rosa_roxburghii Rroxscaffold_5G00384390
rosa_rugosa Rorug04G0347600
rosa_samantha Rh4AG407800 Rh4BG418800 Rh4CG432900 Rh4DG414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 84
AciI CCGC 1 cut(s) 258
AcuI CTGAAG 1 cut(s) 72
AcyI GRCGYC 2 cut(s) 78, 197
AfaI GTAC 3 cut(s) 169, 409, 415
AgsI TTSAA 2 cut(s) 192, 226
AhlI ACTAGT 1 cut(s) 134
AluBI AGCT 2 cut(s) 180, 424
AluI AGCT 2 cut(s) 180, 424
Alw26I GTCTC 1 cut(s) 373
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 3 cut(s) 177, 238, 295
AspS9I GGNCC 1 cut(s) 374
AsuHPI GGTGA 1 cut(s) 86
AvaII GGWCC 1 cut(s) 374
BbsI GAAGAC 1 cut(s) 60
BbvI GCAGC 3 cut(s) 189, 250, 282
BceAI ACGGC 1 cut(s) 210
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BcoDI GTCTC 1 cut(s) 373
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 2 cut(s) 135, 425
BfmI CTRYAG 1 cut(s) 296
BisI GCNGC 3 cut(s) 178, 239, 296
BlsI GCNGC 3 cut(s) 179, 240, 297
Bme18I GGWCC 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 1 cut(s) 41
BmsI GCATC 2 cut(s) 109, 357
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 1 cut(s) 166
Bsa29I ATCGAT 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 147
BsaHI GRCGYC 2 cut(s) 78, 197
BsaI GGTCTC 1 cut(s) 373
BsaJI CCNNGG 1 cut(s) 330
BsaXI ACNNNNNCTCC 1 cut(s) 438
Bse118I RCCGGY 1 cut(s) 241
Bse1I ACTGG 2 cut(s) 296, 411
Bse8I GATNNNNATC 1 cut(s) 147
BseCI ATCGAT 1 cut(s) 436
BseDI CCNNGG 1 cut(s) 330
BseGI GGATG 1 cut(s) 343
BseJI GATNNNNATC 1 cut(s) 147
BseNI ACTGG 2 cut(s) 296, 411
BseRI GAGGAG 1 cut(s) 90
BseXI GCAGC 3 cut(s) 189, 250, 282
BshFI GGCC 1 cut(s) 329
BshVI ATCGAT 1 cut(s) 436
BsiSI CCGG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 373
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 1 cut(s) 329
Bso31I GGTCTC 1 cut(s) 373
Bsp143I GATC 3 cut(s) 88, 142, 433
BspACI CCGC 1 cut(s) 258
BspANI GGCC 1 cut(s) 329
BspDI ATCGAT 1 cut(s) 436
BspHI TCATGA 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 41
BspMAI CTGCAG 1 cut(s) 300
BspTNI GGTCTC 1 cut(s) 373
BsrFI RCCGGY 1 cut(s) 241
BsrI ACTGG 2 cut(s) 296, 411
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 3 cut(s) 88, 142, 433
BssNI GRCGYC 2 cut(s) 78, 197
BssT1I CCWWGG 1 cut(s) 330
BstACI GRCGYC 2 cut(s) 78, 197
BstC8I GCNNGC 2 cut(s) 256, 260
BstF5I GGATG 1 cut(s) 343
BstKTI GATC 3 cut(s) 91, 145, 436
BstMAI GTCTC 1 cut(s) 373
BstMBI GATC 3 cut(s) 88, 142, 433
BstMWI GCNNNNNNNGC 1 cut(s) 279
BstNSI RCATGY 1 cut(s) 126
BstSFI CTRYAG 1 cut(s) 296
BstV1I GCAGC 3 cut(s) 189, 250, 282
BstV2I GAAGAC 1 cut(s) 60
Bsu15I ATCGAT 1 cut(s) 436
BsuRI GGCC 1 cut(s) 329
BsuTUI ATCGAT 1 cut(s) 436
BtsCI GGATG 1 cut(s) 343
BtsI GCAGTG 1 cut(s) 376
BtsIMutI CAGTG 1 cut(s) 376
Cac8I GCNNGC 2 cut(s) 256, 260
CciI TCATGA 1 cut(s) 157
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 374
ClaI ATCGAT 1 cut(s) 436
CseI GACGC 2 cut(s) 186, 354
Csp6I GTAC 3 cut(s) 168, 408, 414
CviAII CATG 2 cut(s) 123, 158
CviJI RGCY 6 cut(s) 49, 180, 241, 295, 329, 424
CviKI_1 RGCY 6 cut(s) 49, 180, 241, 295, 329, 424
CviQI GTAC 3 cut(s) 168, 408, 414
DpnI GATC 3 cut(s) 90, 144, 435
DpnII GATC 3 cut(s) 88, 142, 433
Eco130I CCWWGG 1 cut(s) 330
Eco31I GGTCTC 1 cut(s) 373
Eco47I GGWCC 1 cut(s) 374
Eco57I CTGAAG 1 cut(s) 72
EcoT14I CCWWGG 1 cut(s) 330
EcoT22I ATGCAT 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 2 cut(s) 126, 161
FaiI YATR 4 cut(s) 27, 29, 124, 159
FaqI GGGAC 1 cut(s) 123
FatI CATG 2 cut(s) 122, 157
FauI CCCGC 1 cut(s) 251
FauNDI CATATG 1 cut(s) 27
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 3 cut(s) 178, 239, 296
FokI GGATG 1 cut(s) 350
Fsp4HI GCNGC 3 cut(s) 178, 239, 296
FspBI CTAG 2 cut(s) 135, 425
GluI GCNGC 3 cut(s) 178, 239, 296
GsuI CTGGAG 1 cut(s) 166
HaeIII GGCC 1 cut(s) 329
HapII CCGG 1 cut(s) 242
HgaI GACGC 2 cut(s) 186, 354
Hin1I GRCGYC 2 cut(s) 78, 197
Hin1II CATG 2 cut(s) 126, 161
HinfI GANTC 1 cut(s) 113
HpaII CCGG 1 cut(s) 242
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 2 cut(s) 85, 374
Hpy188I TCNGA 5 cut(s) 109, 118, 232, 309, 357
Hpy188III TCNNGA 3 cut(s) 140, 158, 183
Hpy8I GTNNAC 2 cut(s) 85, 374
Hpy99I CGWCG 3 cut(s) 80, 83, 202
HpyAV CCTTC 1 cut(s) 198
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 4 cut(s) 122, 298, 369, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 279
HpySE526I ACGT 1 cut(s) 78
Hsp92I GRCGYC 2 cut(s) 78, 197
Hsp92II CATG 2 cut(s) 126, 161
Kzo9I GATC 3 cut(s) 88, 142, 433
LmnI GCTCC 1 cut(s) 185
LpnPI CCDG 8 cut(s) 22, 63, 187, 196, 255, 277, 330, 424
Lsp1109I GCAGC 3 cut(s) 189, 250, 282
LweI GCATC 2 cut(s) 109, 357
MaeI CTAG 2 cut(s) 135, 425
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 2 cut(s) 212, 247
MalI GATC 3 cut(s) 90, 144, 435
MboI GATC 3 cut(s) 88, 142, 433
MboII GAAGA 2 cut(s) 65, 122
MfeI CAATTG 1 cut(s) 392
MluCI AATT 1 cut(s) 392
MmeI TCCRAC 1 cut(s) 287
MnlI CCTC 7 cut(s) 27, 77, 108, 111, 162, 228, 319
Mph1103I ATGCAT 1 cut(s) 124
MslI CAYNNNNRTG 2 cut(s) 69, 162
MspI CCGG 1 cut(s) 242
MunI CAATTG 1 cut(s) 392
MwoI GCNNNNNNNGC 1 cut(s) 279
NdeI CATATG 1 cut(s) 27
NdeII GATC 3 cut(s) 88, 142, 433
NlaIII CATG 2 cut(s) 126, 161
NlaIV GGNNCC 1 cut(s) 41
NmuCI GTSAC 1 cut(s) 247
NsiI ATGCAT 1 cut(s) 124
NspI RCATGY 1 cut(s) 126
PagI TCATGA 1 cut(s) 157
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 75, 84
PfeI GAWTC 1 cut(s) 113
PflFI GACNNNGTC 1 cut(s) 77
PkrI GCNGC 3 cut(s) 179, 240, 297
PspN4I GGNNCC 1 cut(s) 41
PspPI GGNCC 1 cut(s) 374
PstI CTGCAG 1 cut(s) 300
PsyI GACNNNGTC 1 cut(s) 77
RsaI GTAC 3 cut(s) 169, 409, 415
RsaNI GTAC 3 cut(s) 168, 408, 414
RseI CAYNNNNRTG 2 cut(s) 69, 162
SatI GCNGC 3 cut(s) 178, 239, 296
Sau3AI GATC 3 cut(s) 88, 142, 433
Sau96I GGNCC 1 cut(s) 374
SetI ASST 7 cut(s) 41, 69, 81, 182, 190, 336, 426
SfaNI GCATC 2 cut(s) 109, 357
SfcI CTRYAG 1 cut(s) 296
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 2 cut(s) 69, 162
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 1 cut(s) 392
SsiI CCGC 1 cut(s) 258
SspMI CTAG 2 cut(s) 135, 425
StyI CCWWGG 1 cut(s) 330
TaiI ACGT 1 cut(s) 81
TaqI TCGA 3 cut(s) 69, 207, 436
TaqII GACCGA 1 cut(s) 391
TasI AATT 1 cut(s) 392
TfiI GAWTC 1 cut(s) 113
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 3 cut(s) 177, 238, 295
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 1 cut(s) 174
TspGWI ACGGA 1 cut(s) 357
TspRI CASTG 1 cut(s) 376
Tth111I GACNNNGTC 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 374
XceI RCATGY 1 cut(s) 126
XmiI GTMKAC 1 cut(s) 84
XspI CTAG 2 cut(s) 135, 425
ZraI GACGTC 1 cut(s) 79
Zsp2I ATGCAT 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.