RchiOBHm_Chr3g0450111

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0010647
Physical Location & Seq
Forward (+)
1748612 .. 1749898
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1287 bp
ATGGCAGTGTTTGAGTGCATTAAGAGAATGATTTATATGTGTATATTGGTTCTCTTCCTGTTCGGCTTCATTGCCGAAACACGAATTGCCCCAACAACAGTCGATGACGACCCCGAGGAAGTTGCTTCCATGGTCTCCTTGAGCATCCAAAACAGCATCGAAAGAAAGCTCAGGCAACTCCAGGGATCATCATTATCATGTGCTACCGGGAATTCCATCGACGACTGTTGGCGATGTGACTCCAATTGGTACAACAATCGGAAGCGCCTCGCGGATTGCGCTATTGGTTTCGGCAGGAACACAATTGGAGGCCGCGACGGCCGCTTCTACATCGTCACTGACTCTAGTGACGACGATGCCGTCAACCTTAAAGTCGGGACCCTTCGCCACGCTGTAATACAATCCGAGCCTCTATGGATTGTGTTCCAGCGTGACATGGTGATCCAGCTCAAGCAGGAGCTTATCATGAATAGCTATAAAACCATCGACGGCCGCGGAGCCAATGTTCACATTGCAAATGAAGCATGCATTACAATCCAAAACGTCACGAACATCATAATACATGGCCTCCACATCCATGATTGCAAGCCTACGGGGAATGCCATGGTGAGGAGCTCACCGTCACACTTTGGTTGGAGAGGAATGGCAGACGGTGATGCTGTGTCGATTTTCGGCTCCAGCAACGTGTGGATTGATCACAACTCACTCTCCAACTGCGCCGACGGCCTCGTGGATGTTGTCATGGCCTCAACTGCAGTTACAATCTCTAACAATTACTTCTCGCACCATAATGATGTCATGTTGTTTGGTCACAGCGACTCTTATACTGGAGACAAGGACATGCAAGTGACGGTTGCTTACAACCACTTTGGGGAAGGACTGATGCAAAGGTTGCCGCATTGTAGGCACGGTTATTGTCATGTGGTGAACAATGACTACAGCCACGGTTGGGGGATATACGCCATCGGTGGAGGTGCAAACCCTACCATCAACAGTGAAGGCAACCGATTTGTTGCTGATCTGAAAAGGTACCCGGATTCGAGTGAGGTTACCAAGAGGACTTCGGGGGACAAGTTGGAGTGGAAGAACTGGAATTGGATTTCTCAAGGAGATTTGTTCTTGAATGGTGCCTACTTTGTTCCGTCTGGAGCAACCGGAGCTAACTCGGAGGCCTATGCTAAGGCCTCGAGCTATGCAAAAGCTAAGAAATCTTCCCTGGTTGTAGCCATAACTTCTTCTGCTGGTGCACTCACCTGCCGCAAAGACCATATGTGCACTAGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

428

Amino Acids

47.17

Weight (kDa)

6.34

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 151 - 360 2.9e-22 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1262
AasI GACNNNNNNGTC 1 cut(s) 359
Acc36I ACCTGC 1 cut(s) 1262
Acc65I GGTACC 1 cut(s) 1029
AccB1I GGYRCC 2 cut(s) 1029, 1127
AccII CGCG 3 cut(s) 272, 315, 495
AciI CCGC 7 cut(s) 272, 313, 322, 493, 495, 896, 1258
AclWI GGATC 2 cut(s) 193, 436
AcoI YGGCCR 2 cut(s) 319, 490
AcsI RAATTY 1 cut(s) 211
AfaI GTAC 2 cut(s) 251, 1031
AfiI CCNNNNNNNGG 3 cut(s) 609, 871, 949
AflIII ACRYGT 1 cut(s) 684
AgsI TTSAA 1 cut(s) 1123
AhlI ACTAGT 1 cut(s) 1277
AjnI CCWGG 2 cut(s) 180, 1215
AloI GAACNNNNNNTCC 2 cut(s) 489, 521
AluBI AGCT 8 cut(s) 169, 448, 460, 474, 615, 1160, 1191, 1202
AluI AGCT 8 cut(s) 169, 448, 460, 474, 615, 1160, 1191, 1202
Alw21I GWGCWC 3 cut(s) 617, 1249, 1277
Alw26I GTCTC 2 cut(s) 139, 825
Alw44I GTGCAC 2 cut(s) 1245, 1273
AlwI GGATC 2 cut(s) 193, 436
Ama87I CYCGRG 2 cut(s) 113, 1186
AoxI GGCC 8 cut(s) 310, 319, 490, 565, 724, 744, 1170, 1182
ApaLI GTGCAC 2 cut(s) 1245, 1273
ApoI RAATTY 1 cut(s) 211
Asp718I GGTACC 1 cut(s) 1029
AspLEI GCGC 3 cut(s) 267, 281, 719
AspS9I GGNCC 1 cut(s) 378
AsuC2I CCSGG 2 cut(s) 208, 1034
AsuHPI GGTGA 6 cut(s) 451, 609, 619, 665, 937, 1243
AvaI CYCGRG 2 cut(s) 113, 1186
AvaII GGWCC 1 cut(s) 378
BaeGI GKGCMC 2 cut(s) 1249, 1277
BanI GGYRCC 2 cut(s) 1029, 1127
BanII GRGCYC 1 cut(s) 617
BauI CACGAG 1 cut(s) 728
Bbv12I GWGCWC 3 cut(s) 617, 1249, 1277
BccI CCATC 4 cut(s) 224, 491, 971, 995
BceAI ACGGC 4 cut(s) 334, 344, 505, 739
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 2 cut(s) 182, 1217
BclI TGATCA 1 cut(s) 694
BcnI CCSGG 2 cut(s) 208, 1034
BcoDI GTCTC 2 cut(s) 139, 825
BcuI ACTAGT 1 cut(s) 1277
BfaI CTAG 3 cut(s) 345, 1278, 1285
BfmI CTRYAG 2 cut(s) 753, 937
BfoI RGCGCY 1 cut(s) 268
BfuAI ACCTGC 1 cut(s) 1262
BglI GCCNNNNNGGC 1 cut(s) 318
BisI GCNGC 5 cut(s) 313, 322, 493, 896, 1258
BlsI GCNGC 5 cut(s) 314, 323, 494, 897, 1259
Bme1390I CCNGG 4 cut(s) 182, 208, 1034, 1217
Bme18I GGWCC 1 cut(s) 378
BmeT110I CYCGRG 2 cut(s) 113, 1186
BmgT120I GGNCC 1 cut(s) 378
BmiI GGNNCC 6 cut(s) 379, 380, 499, 676, 1031, 1129
BmrFI CCNGG 4 cut(s) 182, 208, 1034, 1217
BmsI GCATC 5 cut(s) 153, 165, 346, 646, 873
BpmI CTGGAG 4 cut(s) 164, 661, 849, 1167
Bpu10I CCTNAGC 2 cut(s) 170, 1179
BpuEI CTTGAG 3 cut(s) 160, 434, 1089
BpuMI CCSGG 2 cut(s) 208, 1034
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 7 cut(s) 114, 129, 181, 493, 603, 943, 1215
BsaWI WCCGGW 1 cut(s) 1154
BsaXI ACNNNNNCTCC 6 cut(s) 489, 519, 552, 582, 692, 722
Bsc4I CCNNNNNNNGG 3 cut(s) 609, 871, 949
Bse1I ACTGG 2 cut(s) 832, 1094
Bse3DI GCAATG 2 cut(s) 69, 510
BseBI CCWGG 2 cut(s) 182, 1217
BseDI CCNNGG 7 cut(s) 114, 129, 181, 493, 603, 943, 1215
BseGI GGATG 3 cut(s) 144, 573, 739
BseLI CCNNNNNNNGG 3 cut(s) 609, 871, 949
BseMI GCAATG 2 cut(s) 69, 510
BseMII CTCAG 1 cut(s) 184
BseNI ACTGG 2 cut(s) 832, 1094
BseRI GAGGAG 1 cut(s) 625
BseSI GKGCMC 2 cut(s) 1249, 1277
BseX3I CGGCCG 2 cut(s) 319, 490
Bsh1236I CGCG 3 cut(s) 272, 315, 495
Bsh1285I CGRYCG 2 cut(s) 322, 493
BshFI GGCC 8 cut(s) 312, 321, 492, 567, 726, 746, 1172, 1184
BshNI GGYRCC 2 cut(s) 1029, 1127
BsiEI CGRYCG 2 cut(s) 322, 493
BsiHKAI GWGCWC 3 cut(s) 617, 1249, 1277
BsiHKCI CYCGRG 2 cut(s) 113, 1186
BsiSI CCGG 3 cut(s) 207, 1034, 1155
BslFI GGGAC 2 cut(s) 391, 1082
BslI CCNNNNNNNGG 3 cut(s) 609, 871, 949
BsmAI GTCTC 2 cut(s) 139, 825
BsmFI GGGAC 2 cut(s) 391, 1082
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 8 cut(s) 312, 321, 492, 567, 726, 746, 1172, 1184
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 2 cut(s) 113, 1186
Bsp1286I GDGCHC 3 cut(s) 617, 1249, 1277
Bsp143I GATC 4 cut(s) 185, 441, 694, 1018
Bsp19I CCATGG 2 cut(s) 129, 603
BspACI CCGC 7 cut(s) 272, 313, 322, 493, 495, 896, 1258
BspANI GGCC 8 cut(s) 312, 321, 492, 567, 726, 746, 1172, 1184
BspCNI CTCAG 1 cut(s) 183
BspFNI CGCG 3 cut(s) 272, 315, 495
BspHI TCATGA 1 cut(s) 465
BspLI GGNNCC 6 cut(s) 379, 380, 499, 676, 1031, 1129
BspMAI CTGCAG 1 cut(s) 757
BspMI ACCTGC 1 cut(s) 1262
BspPI GGATC 2 cut(s) 193, 436
BspT107I GGYRCC 2 cut(s) 1029, 1127
BspTNI GGTCTC 1 cut(s) 139
BsrDI GCAATG 2 cut(s) 69, 510
BsrI ACTGG 2 cut(s) 832, 1094
BssECI CCNNGG 7 cut(s) 114, 129, 181, 493, 603, 943, 1215
BssMI GATC 4 cut(s) 185, 441, 694, 1018
BssSI CACGAG 1 cut(s) 728
BssT1I CCWWGG 2 cut(s) 129, 603
Bst2BI CACGAG 1 cut(s) 728
Bst2UI CCWGG 2 cut(s) 182, 1217
Bst4CI ACNGT 8 cut(s) 100, 227, 621, 653, 853, 911, 947, 995
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 892
BstC8I GCNNGC 2 cut(s) 526, 587
BstDEI CTNAG 3 cut(s) 170, 1179, 1203
BstDSI CCRYGG 4 cut(s) 129, 493, 603, 943
BstEII GGTNACC 1 cut(s) 1048
BstF5I GGATG 3 cut(s) 144, 573, 739
BstFNI CGCG 3 cut(s) 272, 315, 495
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 3 cut(s) 267, 281, 719
BstKTI GATC 4 cut(s) 188, 444, 697, 1021
BstMAI GTCTC 2 cut(s) 139, 825
BstMBI GATC 4 cut(s) 185, 441, 694, 1018
BstMCI CGRYCG 2 cut(s) 322, 493
BstMWI GCNNNNNNNGC 9 cut(s) 278, 318, 321, 521, 723, 752, 892, 904, 1157
BstNI CCWGG 2 cut(s) 182, 1217
BstNSI RCATGY 2 cut(s) 528, 844
BstPI GGTNACC 1 cut(s) 1048
BstSCI CCNGG 4 cut(s) 180, 206, 1032, 1215
BstSFI CTRYAG 2 cut(s) 753, 937
BstSLI GKGCMC 2 cut(s) 1249, 1277
BstUI CGCG 3 cut(s) 272, 315, 495
BstZI CGGCCG 2 cut(s) 319, 490
BsuRI GGCC 8 cut(s) 312, 321, 492, 567, 726, 746, 1172, 1184
BtgI CCRYGG 4 cut(s) 129, 493, 603, 943
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 3 cut(s) 144, 573, 739
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 3 cut(s) 12, 336, 1000
BveI ACCTGC 1 cut(s) 1262
Cac8I GCNNGC 2 cut(s) 526, 587
CciI TCATGA 1 cut(s) 465
CfoI GCGC 3 cut(s) 267, 281, 719
Cfr13I GGNCC 1 cut(s) 378
Cfr42I CCGCGG 1 cut(s) 496
Csp6I GTAC 2 cut(s) 250, 1030
CviQI GTAC 2 cut(s) 250, 1030
DdeI CTNAG 3 cut(s) 170, 1179, 1203
DpnI GATC 4 cut(s) 187, 443, 696, 1020
DpnII GATC 4 cut(s) 185, 441, 694, 1018
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 2 cut(s) 319, 490
EagI CGGCCG 2 cut(s) 319, 490
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 615
EclXI CGGCCG 2 cut(s) 319, 490
Eco130I CCWWGG 2 cut(s) 129, 603
Eco147I AGGCCT 2 cut(s) 1172, 1184
Eco24I GRGCYC 1 cut(s) 617
Eco31I GGTCTC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 378
Eco52I CGGCCG 2 cut(s) 319, 490
Eco53kI GAGCTC 1 cut(s) 615
Eco88I CYCGRG 2 cut(s) 113, 1186
Eco91I GGTNACC 1 cut(s) 1048
EcoICRI GAGCTC 1 cut(s) 615
EcoO109I RGGNCCY 1 cut(s) 378
EcoO65I GGTNACC 1 cut(s) 1048
EcoRI GAATTC 1 cut(s) 211
EcoRII CCWGG 2 cut(s) 180, 1215
EcoT14I CCWWGG 2 cut(s) 129, 603
EcoT22I ATGCAT 1 cut(s) 530
EcoT38I GRGCYC 1 cut(s) 617
ErhI CCWWGG 2 cut(s) 129, 603
FaqI GGGAC 2 cut(s) 391, 1082
FauNDI CATATG 1 cut(s) 1269
FbaI TGATCA 1 cut(s) 694
Fnu4HI GCNGC 5 cut(s) 313, 322, 493, 896, 1258
FokI GGATG 3 cut(s) 131, 560, 746
FriOI GRGCYC 1 cut(s) 617
Fsp4HI GCNGC 5 cut(s) 313, 322, 493, 896, 1258
FspBI CTAG 3 cut(s) 345, 1278, 1285
GlaI GCGC 3 cut(s) 266, 280, 718
GluI GCNGC 5 cut(s) 313, 322, 493, 896, 1258
GsuI CTGGAG 4 cut(s) 164, 661, 849, 1167
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 8 cut(s) 312, 321, 492, 567, 726, 746, 1172, 1184
HapII CCGG 3 cut(s) 207, 1034, 1155
HhaI GCGC 3 cut(s) 267, 281, 719
Hin6I GCGC 3 cut(s) 265, 279, 717
HinP1I GCGC 3 cut(s) 265, 279, 717
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 4 cut(s) 239, 341, 818, 1037
HpaII CCGG 3 cut(s) 207, 1034, 1155
HphI GGTGA 6 cut(s) 451, 609, 619, 665, 937, 1243
Hpy166II GTNNAC 5 cut(s) 364, 508, 928, 1247, 1275
Hpy188I TCNGA 4 cut(s) 261, 406, 1023, 1168
Hpy188III TCNNGA 5 cut(s) 376, 466, 547, 1120, 1146
Hpy8I GTNNAC 5 cut(s) 364, 508, 928, 1247, 1275
Hpy99I CGWCG 5 cut(s) 224, 320, 356, 491, 725
HpyAV CCTTC 3 cut(s) 392, 869, 992
HpyCH4III ACNGT 8 cut(s) 100, 227, 621, 653, 853, 911, 947, 995
HpyCH4IV ACGT 2 cut(s) 543, 684
HpyF10VI GCNNNNNNNGC 9 cut(s) 278, 318, 321, 521, 723, 752, 892, 904, 1157
HpyF3I CTNAG 3 cut(s) 170, 1179, 1203
HpySE526I ACGT 2 cut(s) 543, 684
HspAI GCGC 3 cut(s) 265, 279, 717
KflI GGGWCCC 1 cut(s) 378
KpnI GGTACC 1 cut(s) 1033
Ksp22I TGATCA 1 cut(s) 694
KspI CCGCGG 1 cut(s) 496
Kzo9I GATC 4 cut(s) 185, 441, 694, 1018
LmnI GCTCC 6 cut(s) 457, 497, 612, 680, 1148, 1157
LweI GCATC 5 cut(s) 153, 165, 346, 646, 873
MaeI CTAG 3 cut(s) 345, 1278, 1285
MaeII ACGT 2 cut(s) 543, 684
MalI GATC 4 cut(s) 187, 443, 696, 1020
MboI GATC 4 cut(s) 185, 441, 694, 1018
MboII GAAGA 4 cut(s) 46, 1096, 1203, 1227
MfeI CAATTG 2 cut(s) 244, 303
MhlI GDGCHC 3 cut(s) 617, 1249, 1277
MluCI AATT 6 cut(s) 84, 211, 244, 303, 772, 1093
MlyI GAGTC 3 cut(s) 233, 335, 812
MmeI TCCRAC 3 cut(s) 614, 735, 1056
Mph1103I ATGCAT 1 cut(s) 530
MseI TTAA 2 cut(s) 21, 369
MslI CAYNNNNRTG 5 cut(s) 196, 576, 789, 792, 845
MspA1I CMGCKG 1 cut(s) 495
MspI CCGG 3 cut(s) 207, 1034, 1155
MspR9I CCNGG 4 cut(s) 182, 208, 1034, 1217
MunI CAATTG 2 cut(s) 244, 303
Mva1269I GAATGC 1 cut(s) 604
MvaI CCWGG 2 cut(s) 182, 1217
MvnI CGCG 3 cut(s) 272, 315, 495
MwoI GCNNNNNNNGC 9 cut(s) 278, 318, 321, 521, 723, 752, 892, 904, 1157
NciI CCSGG 2 cut(s) 208, 1034
NcoI CCATGG 2 cut(s) 129, 603
NdeI CATATG 1 cut(s) 1269
NdeII GATC 4 cut(s) 185, 441, 694, 1018
NlaIV GGNNCC 6 cut(s) 379, 380, 499, 676, 1031, 1129
NmuCI GTSAC 8 cut(s) 236, 334, 347, 431, 544, 621, 809, 847
NsiI ATGCAT 1 cut(s) 530
NspI RCATGY 2 cut(s) 528, 844
PaeI GCATGC 1 cut(s) 528
PaeR7I CTCGAG 1 cut(s) 1186
PagI TCATGA 1 cut(s) 465
PaqCI CACCTGC 1 cut(s) 1262
PceI AGGCCT 2 cut(s) 1172, 1184
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PctI GAATGC 1 cut(s) 604
PfeI GAWTC 1 cut(s) 1037
PkrI GCNGC 5 cut(s) 314, 323, 494, 897, 1259
PleI GAGTC 3 cut(s) 233, 335, 812
PpsI GAGTC 3 cut(s) 233, 335, 812
PpuMI RGGWCCY 1 cut(s) 378
Psp124BI GAGCTC 1 cut(s) 617
Psp5II RGGWCCY 1 cut(s) 378
Psp6I CCWGG 2 cut(s) 180, 1215
PspEI GGTNACC 1 cut(s) 1048
PspGI CCWGG 2 cut(s) 180, 1215
PspN4I GGNNCC 6 cut(s) 379, 380, 499, 676, 1031, 1129
PspPI GGNCC 1 cut(s) 378
PspPPI RGGWCCY 1 cut(s) 378
PspXI VCTCGAGB 1 cut(s) 1186
PsrI GAACNNNNNNTAC 2 cut(s) 920, 952
PstI CTGCAG 1 cut(s) 757
RsaI GTAC 2 cut(s) 251, 1031
RsaNI GTAC 2 cut(s) 250, 1030
RseI CAYNNNNRTG 5 cut(s) 196, 576, 789, 792, 845
SacI GAGCTC 1 cut(s) 617
SacII CCGCGG 1 cut(s) 496
SaqAI TTAA 2 cut(s) 21, 369
SatI GCNGC 5 cut(s) 313, 322, 493, 896, 1258
Sau3AI GATC 4 cut(s) 185, 441, 694, 1018
Sau96I GGNCC 1 cut(s) 378
SchI GAGTC 3 cut(s) 233, 335, 812
ScrFI CCNGG 4 cut(s) 182, 208, 1034, 1217
SduI GDGCHC 3 cut(s) 617, 1249, 1277
SfaNI GCATC 5 cut(s) 153, 165, 346, 646, 873
SfcI CTRYAG 2 cut(s) 753, 937
SfiI GGCCNNNNNGGCC 1 cut(s) 318
Sfr274I CTCGAG 1 cut(s) 1186
Sfr303I CCGCGG 1 cut(s) 496
SgrBI CCGCGG 1 cut(s) 496
SinI GGWCC 1 cut(s) 378
SlaI CTCGAG 1 cut(s) 1186
SmiMI CAYNNNNRTG 5 cut(s) 196, 576, 789, 792, 845
SmlI CTYRAG 4 cut(s) 139, 449, 1104, 1186
SmoI CTYRAG 4 cut(s) 139, 449, 1104, 1186
SpeI ACTAGT 1 cut(s) 1277
SphI GCATGC 1 cut(s) 528
Sse9I AATT 6 cut(s) 84, 211, 244, 303, 772, 1093
SseBI AGGCCT 2 cut(s) 1172, 1184
SsiI CCGC 7 cut(s) 272, 313, 322, 493, 495, 896, 1258
SspMI CTAG 3 cut(s) 345, 1278, 1285
SstI GAGCTC 1 cut(s) 617
StuI AGGCCT 2 cut(s) 1172, 1184
StyD4I CCNGG 4 cut(s) 180, 206, 1032, 1215
StyI CCWWGG 2 cut(s) 129, 603
TaaI ACNGT 8 cut(s) 100, 227, 621, 653, 853, 911, 947, 995
TaiI ACGT 2 cut(s) 546, 687
TaqI TCGA 7 cut(s) 102, 159, 219, 486, 665, 1040, 1187
TasI AATT 6 cut(s) 84, 211, 244, 303, 772, 1093
TauI GCSGC 5 cut(s) 315, 324, 495, 898, 1260
TfiI GAWTC 1 cut(s) 1037
Tru1I TTAA 2 cut(s) 21, 369
Tru9I TTAA 2 cut(s) 21, 369
TscAI CASTG 3 cut(s) 12, 343, 1000
TseFI GTSAC 8 cut(s) 236, 334, 347, 431, 544, 621, 809, 847
Tsp45I GTSAC 8 cut(s) 236, 334, 347, 431, 544, 621, 809, 847
TspDTI ATGAA 3 cut(s) 58, 482, 534
TspGWI ACGGA 1 cut(s) 1131
TspRI CASTG 3 cut(s) 12, 343, 1000
VneI GTGCAC 2 cut(s) 1245, 1273
VpaK11BI GGWCC 1 cut(s) 378
XapI RAATTY 1 cut(s) 211
XceI RCATGY 2 cut(s) 528, 844
XhoI CTCGAG 1 cut(s) 1186
XspI CTAG 3 cut(s) 345, 1278, 1285
Zsp2I ATGCAT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.