RchiOBHm_Chr3g0479581

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0017431
Physical Location & Seq
Reverse (-)
25413843 .. 25415032
1190 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1110 bp
ATGGCGATGCGAGGCAAAATTAGGGAATCGACAAAAGTTAAAAGATACTGGCCTGGAAAAGCCCCAGAGTGGGCAAACGATCGTGATACCTGCTCCTCCGACGAAGAAGGGATTACCATCTTGTACAAATCCTTCTCAACTCGTGATGATGATCCAAGACTACGTCGTTTGGCCGAGAGGAGGAGTGTAAAGAGGCGCATCCGCGGTGCCAAGATTGTTGACACAACTGAAGAGGAGGAGGAGGAGGAAGAAGAAGAGGACGCAGAGACTTTGGAGGAAAGAAGAAGAAGGATTAAGGAGAGGTTGCTTCGTCAGAGGGAGCAGGAACAACAACAAGAGGAGTTTGAGACTGATGACTACTACTCGGAAGATGAATCCGATGAGGACTACGAAGAAGAAGAAGAAGAGGAGGAGGATGAGATTGCTGGTGAAGTAGTTACAGTGAAGCCTGTATTTTTGTCCAAGTCCACAAGAGACACGATAGTTGAACGTAAGAGACTTGAGGAGGCCGAAGAGCAGGCCCTGGAAGCGAGGAGAAGGGAGCTGGAGGAGAGGAAGAAAGAGACAAGGAGAATGGTGCTCGAAGAGATTCGGAAAGACGAAGAGATTCACAAGGGCAAGAGAGAGGGCCAGGCTCAAGAAGGAGAGGGCAGCAAGGGAAGCAGTCATTTTGAAGGAGAGAAGATGAATGATCAAGAGAGGAAGGAGTACTGGGAGAGGAAGAATCCTCCCTCACCTCCTGCGCCACAGCCCCGAAAGGGGAAGCTTAAGTTCATGCAGAGAGATCACCACAGGGGAGCTTTCTTCCGTTCGGACTCTGATGATGAATATGGCGTTTTAGGAAGAGATTATTCTGCCCCAACTGGAGAGGATAAAATGGATAAAACAAGACTTCCCAAGGTGATGCAGGTCAAGAATTTCGGGCGTAGGGGAAGGGCTAAATGGACTCATCTTGTCAATGAGGATACTACTGATTGGAACAGCCCGTGGATGTGGAATGATCGTCAAAGGTCAAGGTATAATGTGAAAATGGCAGCCATGAATGCACCAATAGCAAGACCAAAAGGAAACAAGAGATTGAGGTTCTCTGTGGAGGAAGATGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

369

Amino Acids

44.11

Weight (kDa)

5.28

Isoelectric Point (pI)

73.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 137 - 239 6.7e-09 Microfibril-associated/Pre-mRNA processing
MFAP1 PF06991 203 - 323 1.6e-29 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017431)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479581
rosa_laevigata RLG00000023558 RLG00000029558 RLG00000029584
rosa_multiflora Rmu_sc0002232.1_g000002 Rmu_sc0007129.1_g000020
rosa_roxburghii Rroxscaffold_6G00402140
rosa_rugosa Rorug03G0176600
rosa_samantha Rh3BG260500 Rh3CG257300 Rh3CG257900
rosa_wichuraiana Rw3G020310 Rw3G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 98, 898
AccB1I GGYRCC 1 cut(s) 206
AccII CGCG 1 cut(s) 204
AciI CCGC 2 cut(s) 202, 204
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 916
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 2 cut(s) 125, 710
AfiI CCNNNNNNNGG 5 cut(s) 69, 70, 180, 758, 759
AflII CTTAAG 1 cut(s) 767
AgsI TTSAA 2 cut(s) 488, 674
AjnI CCWGG 3 cut(s) 52, 522, 630
AluBI AGCT 3 cut(s) 544, 766, 800
AluI AGCT 3 cut(s) 544, 766, 800
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 5 cut(s) 260, 341, 468, 490, 557
AlwI GGATC 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 523
AoxI GGCC 5 cut(s) 50, 171, 507, 519, 628
ApeKI GCWGC 2 cut(s) 651, 1034
ApoI RAATTY 1 cut(s) 916
Asp700I GAANNNNTTC 2 cut(s) 588, 606
AspLEI GCGC 2 cut(s) 198, 745
AspS9I GGNCC 2 cut(s) 520, 628
AsuHPI GGTGA 4 cut(s) 440, 726, 779, 913
BanI GGYRCC 1 cut(s) 206
BarI GAAGNNNNNNTAC 2 cut(s) 116, 148
BauI CACGAG 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 2 cut(s) 663, 1046
BccI CCATC 1 cut(s) 125
BciT130I CCWGG 3 cut(s) 54, 524, 632
BciVI GTATCC 1 cut(s) 958
BclI TGATCA 1 cut(s) 691
BcoDI GTCTC 5 cut(s) 260, 341, 468, 490, 557
BfrI CTTAAG 1 cut(s) 767
BfuAI ACCTGC 2 cut(s) 98, 898
BfuI GTATCC 1 cut(s) 958
BisI GCNGC 2 cut(s) 652, 1035
BlsI GCNGC 2 cut(s) 653, 1036
BmcAI AGTACT 1 cut(s) 710
Bme1390I CCNGG 3 cut(s) 54, 524, 632
BmgT120I GGNCC 2 cut(s) 520, 628
BmiI GGNNCC 1 cut(s) 208
BmrFI CCNGG 3 cut(s) 54, 524, 632
BmrI ACTGGG 1 cut(s) 721
BmsI GCATC 2 cut(s) 207, 894
BmuI ACTGGG 1 cut(s) 721
BpmI CTGGAG 2 cut(s) 566, 885
BpuEI CTTGAG 2 cut(s) 521, 621
BsaBI GATNNNNATC 2 cut(s) 116, 150
BsaJI CCNNGG 4 cut(s) 202, 522, 897, 986
Bsc4I CCNNNNNNNGG 5 cut(s) 69, 70, 180, 758, 759
Bse1I ACTGG 3 cut(s) 53, 716, 868
Bse8I GATNNNNATC 2 cut(s) 116, 150
BseBI CCWGG 3 cut(s) 54, 524, 632
BseDI CCNNGG 4 cut(s) 202, 522, 897, 986
BseGI GGATG 3 cut(s) 198, 421, 996
BseJI GATNNNNATC 2 cut(s) 116, 150
BseLI CCNNNNNNNGG 5 cut(s) 69, 70, 180, 758, 759
BseNI ACTGG 3 cut(s) 53, 716, 868
BseXI GCAGC 2 cut(s) 663, 1046
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 5 cut(s) 52, 173, 509, 521, 630
BshNI GGYRCC 1 cut(s) 206
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 582
BslI CCNNNNNNNGG 5 cut(s) 69, 70, 180, 758, 759
BsmAI GTCTC 5 cut(s) 260, 341, 468, 490, 557
BsmI GAATGC 1 cut(s) 1048
BsnI GGCC 5 cut(s) 52, 173, 509, 521, 630
Bsp1286I GDGCHC 1 cut(s) 582
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 5 cut(s) 79, 151, 691, 784, 1000
BspACI CCGC 2 cut(s) 202, 204
BspANI GGCC 5 cut(s) 52, 173, 509, 521, 630
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 208
BspMI ACCTGC 2 cut(s) 98, 898
BspPI GGATC 1 cut(s) 146
BspQI GCTCTTC 1 cut(s) 507
BspT107I GGYRCC 1 cut(s) 206
BspTI CTTAAG 1 cut(s) 767
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 3 cut(s) 53, 716, 868
BssECI CCNNGG 4 cut(s) 202, 522, 897, 986
BssMI GATC 5 cut(s) 79, 151, 691, 784, 1000
BssSI CACGAG 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 897
Bst2BI CACGAG 1 cut(s) 141
Bst2UI CCWGG 3 cut(s) 54, 524, 632
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 7 cut(s) 225, 249, 399, 507, 579, 597, 838
BstAFI CTTAAG 1 cut(s) 767
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 519
BstDSI CCRYGG 2 cut(s) 202, 986
BstF5I GGATG 3 cut(s) 198, 421, 996
BstFNI CGCG 1 cut(s) 204
BstHHI GCGC 2 cut(s) 198, 745
BstKTI GATC 5 cut(s) 82, 154, 694, 787, 1003
BstMAI GTCTC 5 cut(s) 260, 341, 468, 490, 557
BstMBI GATC 5 cut(s) 79, 151, 691, 784, 1000
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 4 cut(s) 527, 660, 1043, 1052
BstNI CCWGG 3 cut(s) 54, 524, 632
BstSCI CCNGG 3 cut(s) 52, 522, 630
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 2 cut(s) 663, 1046
BsuI GTATCC 1 cut(s) 958
BsuRI GGCC 5 cut(s) 52, 173, 509, 521, 630
BtgI CCRYGG 2 cut(s) 202, 986
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 3 cut(s) 198, 421, 996
BtsIMutI CAGTG 1 cut(s) 447
BveI ACCTGC 2 cut(s) 98, 898
Cac8I GCNNGC 1 cut(s) 519
CaiI CAGNNNCTG 1 cut(s) 523
CfoI GCGC 2 cut(s) 198, 745
Cfr13I GGNCC 2 cut(s) 520, 628
Cfr42I CCGCGG 1 cut(s) 205
CseI GACGC 1 cut(s) 269
Csp6I GTAC 2 cut(s) 124, 709
CviAII CATG 2 cut(s) 775, 1039
CviQI GTAC 2 cut(s) 124, 709
DpnI GATC 5 cut(s) 81, 153, 693, 786, 1002
DpnII GATC 5 cut(s) 79, 151, 691, 784, 1000
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 7 cut(s) 225, 249, 399, 507, 579, 597, 838
EarI CTCTTC 7 cut(s) 225, 249, 399, 507, 579, 597, 838
Eco130I CCWWGG 1 cut(s) 897
Eco57I CTGAAG 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 520
EcoRII CCWGG 3 cut(s) 52, 522, 630
EcoT14I CCWWGG 1 cut(s) 897
ErhI CCWWGG 1 cut(s) 897
FaeI CATG 2 cut(s) 778, 1042
FaiI YATR 4 cut(s) 776, 831, 1020, 1040
FalI AAGNNNNNCTT 1 cut(s) 1089
FatI CATG 2 cut(s) 774, 1038
FbaI TGATCA 1 cut(s) 691
Fnu4HI GCNGC 2 cut(s) 652, 1035
FokI GGATG 3 cut(s) 185, 428, 1003
Fsp4HI GCNGC 2 cut(s) 652, 1035
GlaI GCGC 2 cut(s) 197, 744
GluI GCNGC 2 cut(s) 652, 1035
GsuI CTGGAG 2 cut(s) 566, 885
HaeIII GGCC 5 cut(s) 52, 173, 509, 521, 630
HgaI GACGC 1 cut(s) 269
HhaI GCGC 2 cut(s) 198, 745
Hin1II CATG 2 cut(s) 778, 1042
Hin6I GCGC 2 cut(s) 196, 743
HinP1I GCGC 2 cut(s) 196, 743
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 7 cut(s) 26, 374, 589, 607, 724, 815, 946
HphI GGTGA 4 cut(s) 440, 726, 779, 913
Hpy166II GTNNAC 2 cut(s) 220, 468
Hpy188I TCNGA 7 cut(s) 100, 315, 367, 379, 594, 814, 820
Hpy188III TCNNGA 5 cut(s) 83, 143, 638, 695, 913
Hpy8I GTNNAC 2 cut(s) 220, 468
Hpy99I CGWCG 2 cut(s) 104, 168
HpyAV CCTTC 8 cut(s) 101, 142, 282, 531, 635, 668, 697, 927
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 2 cut(s) 163, 490
HpyCH4V TGCA 3 cut(s) 778, 907, 1046
HpyF10VI GCNNNNNNNGC 4 cut(s) 527, 660, 1043, 1052
HpySE526I ACGT 2 cut(s) 163, 490
Hsp92II CATG 2 cut(s) 778, 1042
HspAI GCGC 2 cut(s) 196, 743
Ksp22I TGATCA 1 cut(s) 691
KspI CCGCGG 1 cut(s) 205
Kzo9I GATC 5 cut(s) 79, 151, 691, 784, 1000
LguI GCTCTTC 1 cut(s) 507
LmnI GCTCC 4 cut(s) 98, 319, 541, 797
Lsp1109I GCAGC 2 cut(s) 663, 1046
LweI GCATC 2 cut(s) 207, 894
MaeII ACGT 2 cut(s) 163, 490
MaeIII GTNAC 1 cut(s) 436
MalI GATC 5 cut(s) 81, 153, 693, 786, 1002
MboI GATC 5 cut(s) 79, 151, 691, 784, 1000
MhlI GDGCHC 1 cut(s) 582
MluCI AATT 2 cut(s) 18, 916
MlyI GAGTC 2 cut(s) 809, 940
MmeI TCCRAC 1 cut(s) 123
MroXI GAANNNNTTC 2 cut(s) 588, 606
MseI TTAA 4 cut(s) 39, 294, 768, 1108
MspA1I CMGCKG 1 cut(s) 204
MspCI CTTAAG 1 cut(s) 767
MspR9I CCNGG 3 cut(s) 54, 524, 632
Mva1269I GAATGC 1 cut(s) 1048
MvaI CCWGG 3 cut(s) 54, 524, 632
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 4 cut(s) 527, 660, 1043, 1052
NdeII GATC 5 cut(s) 79, 151, 691, 784, 1000
NlaIII CATG 2 cut(s) 778, 1042
NlaIV GGNNCC 1 cut(s) 208
NmeAIII GCCGAG 1 cut(s) 199
PciSI GCTCTTC 1 cut(s) 507
PctI GAATGC 1 cut(s) 1048
PdmI GAANNNNTTC 2 cut(s) 588, 606
PfeI GAWTC 5 cut(s) 26, 374, 589, 607, 724
PflFI GACNNNGTC 1 cut(s) 162
PkrI GCNGC 2 cut(s) 653, 1036
Ple19I CGATCG 1 cut(s) 82
PleI GAGTC 2 cut(s) 809, 940
PpsI GAGTC 2 cut(s) 809, 940
Psp6I CCWGG 3 cut(s) 52, 522, 630
PspGI CCWGG 3 cut(s) 52, 522, 630
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 520, 628
PstNI CAGNNNCTG 1 cut(s) 523
PsyI GACNNNGTC 1 cut(s) 162
PvuI CGATCG 1 cut(s) 82
RsaI GTAC 2 cut(s) 125, 710
RsaNI GTAC 2 cut(s) 124, 709
SacII CCGCGG 1 cut(s) 205
SapI GCTCTTC 1 cut(s) 507
SaqAI TTAA 4 cut(s) 39, 294, 768, 1108
SatI GCNGC 2 cut(s) 652, 1035
Sau3AI GATC 5 cut(s) 79, 151, 691, 784, 1000
Sau96I GGNCC 2 cut(s) 520, 628
ScaI AGTACT 1 cut(s) 710
SchI GAGTC 2 cut(s) 809, 940
ScrFI CCNGG 3 cut(s) 54, 524, 632
SduI GDGCHC 1 cut(s) 582
SfaNI GCATC 2 cut(s) 207, 894
Sfr303I CCGCGG 1 cut(s) 205
SgrBI CCGCGG 1 cut(s) 205
SmlI CTYRAG 3 cut(s) 500, 636, 767
SmoI CTYRAG 3 cut(s) 500, 636, 767
Sse9I AATT 2 cut(s) 18, 916
SsiI CCGC 2 cut(s) 202, 204
StyD4I CCNGG 3 cut(s) 52, 522, 630
StyI CCWWGG 1 cut(s) 897
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 2 cut(s) 166, 493
TaqI TCGA 2 cut(s) 29, 582
TasI AATT 2 cut(s) 18, 916
TatI WGTACW 2 cut(s) 123, 708
TfiI GAWTC 5 cut(s) 26, 374, 589, 607, 724
Tru1I TTAA 4 cut(s) 39, 294, 768, 1108
Tru9I TTAA 4 cut(s) 39, 294, 768, 1108
TscAI CASTG 1 cut(s) 447
TseI GCWGC 2 cut(s) 651, 1034
TspDTI ATGAA 5 cut(s) 387, 701, 763, 840, 1055
TspGWI ACGGA 1 cut(s) 797
TspRI CASTG 1 cut(s) 447
Tth111I GACNNNGTC 1 cut(s) 162
Vha464I CTTAAG 1 cut(s) 767
XapI RAATTY 1 cut(s) 916
XmnI GAANNNNTTC 2 cut(s) 588, 606
ZrmI AGTACT 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.