Rh3BG260500

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0017431
Physical Location & Seq
Reverse (-)
25261266 .. 25262621
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG260500.1

Sequence Viewer

Length: 1203 bp
ATGGCGATGCGAGGCAAAATTAGGGAATCGACAAAAGTTAATAGACACTGGCCTGGAAAAGCCCCAGAGTGGGCAAATGATCGTGATACCTGCTCCTCCGACGAAGAAGGGATTACCATCTTGTACAAATCCTTCTCAACTCGTGATGATGATCCTAGACTACGTCGTTTGGCCGAGAGTAGGAGTGTAAAGAGGCGCTTCCGCCATGCCGAGATTGTTGAAACAACTGAAGAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGAGGACGCAGAGACTTTCGAGGAAAGAAGAAGAAGGATTAAGGAGAGGTTGCTTCGTCAGAGGGAGCAGAAACAACAAGAGGAGTTTGAGACTGATGACTACTGCTCGGAAGACGAATCCGATGAGGACTACTCAGAAGAAGAAGAAGAAGAAGAAGAAGAGGATGAGATTGCTGGTGAAGTAGTTACGGTGAAGCCCGTATTTTTGTCCAAGTCCACAAGAGACACGATAGTTGAACGTAAGAGACTTGAGGAGGCCGAAGAGCAGGCCCTGGAAGCGAGGAGAAGGGAGCTGGAGGAGAGGAAGAAAGAGACAAAGAGAATGGTGCTCGAAGAGATTCGGAAAGACGAAGAGATTCACAAGGGTTTGATGAATCAGGATTCGGCAAATGATGATGTTGACACTGACAATGATCCTGAGGAGGATACTAAAGCAGCTTGGAAGGCAAGAGAGAGGGCCAGGCTCAAGAAGGAGAGGGCAGCAAGGGAAGCAGTCATTTTGAAGGAGATAGAGAAGATGAATGATCAAGAGAGGAGGGAGTACTGGGAGAGGAAGAATCCTGCCTCACCTCCTGCGCCACAGCCCCGAAAGAGGAAGATTAAGTTCATGCAGAGAGATCACCACAGGGGAGCTTTCTTCCGTTCGGACTCTGATGATGAAGATGGCGTTTTAGAAAGAGATTATTCGGCCCCAACCGGAGAGGATAAGATGGATAAAACAAGACTGCCCAAGGTGATGCAGGTCAAGAATTTCGGGCGTAGGGGAAAGGCTAAATGGACTCATCTTGTCAATGAGGATACTACTGATTGGGACAGCCCGTGGATGTGGAATGATCGTCAAGGGTCAAGGTATAATGTGAAAATGGCAGCCATGAATGCACCAATAGCAAGACCAAAAGGAAACAAGAGATTGAGGTTCTCTGTGGATGAAGATGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

400

Amino Acids

47.83

Weight (kDa)

5.02

Isoelectric Point (pI)

75.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 140 - 354 2.9e-51 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017431)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479581
rosa_laevigata RLG00000023558 RLG00000029558 RLG00000029584
rosa_multiflora Rmu_sc0002232.1_g000002 Rmu_sc0007129.1_g000020
rosa_roxburghii Rroxscaffold_6G00402140
rosa_rugosa Rorug03G0176600
rosa_samantha Rh3BG260500 Rh3CG257300 Rh3CG257900
rosa_wichuraiana Rw3G020310 Rw3G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 98, 991
AciI CCGC 1 cut(s) 202
AclWI GGATC 2 cut(s) 146, 668
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 1009
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 2 cut(s) 125, 803
AfiI CCNNNNNNNGG 4 cut(s) 69, 70, 180, 852
AgsI TTSAA 3 cut(s) 221, 497, 763
AjnI CCWGG 3 cut(s) 52, 531, 719
AluBI AGCT 3 cut(s) 553, 698, 893
AluI AGCT 3 cut(s) 553, 698, 893
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 5 cut(s) 266, 344, 477, 499, 566
AlwI GGATC 2 cut(s) 146, 668
AlwNI CAGNNNCTG 1 cut(s) 532
AoxI GGCC 6 cut(s) 50, 171, 516, 528, 717, 948
ApeKI GCWGC 3 cut(s) 695, 740, 1127
ApoI RAATTY 1 cut(s) 1009
Asp700I GAANNNNTTC 2 cut(s) 597, 615
AspLEI GCGC 2 cut(s) 198, 838
AspS9I GGNCC 3 cut(s) 529, 717, 949
AsuHPI GGTGA 5 cut(s) 449, 463, 819, 872, 1006
AxyI CCTNAGG 1 cut(s) 678
BarI GAAGNNNNNNTAC 2 cut(s) 116, 148
BauI CACGAG 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 3 cut(s) 707, 752, 1139
BccI CCATC 3 cut(s) 125, 917, 964
BciT130I CCWGG 3 cut(s) 54, 533, 721
BciVI GTATCC 2 cut(s) 679, 1051
BclI TGATCA 1 cut(s) 784
BcoDI GTCTC 5 cut(s) 266, 344, 477, 499, 566
BfaI CTAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 199
BfuAI ACCTGC 2 cut(s) 98, 991
BfuI GTATCC 2 cut(s) 679, 1051
BisI GCNGC 3 cut(s) 696, 741, 1128
BlsI GCNGC 3 cut(s) 697, 742, 1129
BmcAI AGTACT 1 cut(s) 803
Bme1390I CCNGG 3 cut(s) 54, 533, 721
BmgT120I GGNCC 3 cut(s) 529, 717, 949
BmiI GGNNCC 1 cut(s) 951
BmrFI CCNGG 3 cut(s) 54, 533, 721
BmrI ACTGGG 1 cut(s) 814
BmsI GCATC 1 cut(s) 987
BmuI ACTGGG 1 cut(s) 814
BpiI GAAGAC 1 cut(s) 378
BplI GAGNNNNNCTC 2 cut(s) 377, 409
BpmI CTGGAG 1 cut(s) 575
BpuEI CTTGAG 2 cut(s) 530, 710
BsaBI GATNNNNATC 2 cut(s) 116, 150
BsaJI CCNNGG 3 cut(s) 531, 990, 1079
BsaWI WCCGGW 1 cut(s) 956
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 70, 180, 852
Bse1I ACTGG 2 cut(s) 53, 809
Bse21I CCTNAGG 1 cut(s) 678
Bse8I GATNNNNATC 2 cut(s) 116, 150
BseBI CCWGG 3 cut(s) 54, 533, 721
BseDI CCNNGG 3 cut(s) 531, 990, 1079
BseGI GGATG 3 cut(s) 430, 1089, 1192
BseJI GATNNNNATC 2 cut(s) 116, 150
BseLI CCNNNNNNNGG 4 cut(s) 69, 70, 180, 852
BseMII CTCAG 2 cut(s) 408, 669
BseNI ACTGG 2 cut(s) 53, 809
BseXI GCAGC 3 cut(s) 707, 752, 1139
BshFI GGCC 6 cut(s) 52, 173, 518, 530, 719, 950
BsiHKAI GWGCWC 1 cut(s) 591
BsiSI CCGG 1 cut(s) 957
BslFI GGGAC 1 cut(s) 1085
BslI CCNNNNNNNGG 4 cut(s) 69, 70, 180, 852
BsmAI GTCTC 5 cut(s) 266, 344, 477, 499, 566
BsmFI GGGAC 1 cut(s) 1085
BsmI GAATGC 1 cut(s) 1141
BsnI GGCC 6 cut(s) 52, 173, 518, 530, 719, 950
Bsp1286I GDGCHC 1 cut(s) 591
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 6 cut(s) 79, 151, 673, 784, 877, 1093
BspACI CCGC 1 cut(s) 202
BspANI GGCC 6 cut(s) 52, 173, 518, 530, 719, 950
BspCNI CTCAG 2 cut(s) 407, 670
BspLI GGNNCC 1 cut(s) 951
BspMI ACCTGC 2 cut(s) 98, 991
BspPI GGATC 2 cut(s) 146, 668
BspQI GCTCTTC 1 cut(s) 516
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 2 cut(s) 53, 809
BssECI CCNNGG 3 cut(s) 531, 990, 1079
BssMI GATC 6 cut(s) 79, 151, 673, 784, 877, 1093
BssSI CACGAG 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 990
Bst2BI CACGAG 1 cut(s) 141
Bst2UI CCWGG 3 cut(s) 54, 533, 721
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 6 cut(s) 225, 255, 414, 516, 588, 606
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 2 cut(s) 394, 678
BstDSI CCRYGG 1 cut(s) 1079
BstF5I GGATG 3 cut(s) 430, 1089, 1192
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 2 cut(s) 198, 838
BstKTI GATC 6 cut(s) 82, 154, 676, 787, 880, 1096
BstMAI GTCTC 5 cut(s) 266, 344, 477, 499, 566
BstMBI GATC 6 cut(s) 79, 151, 673, 784, 877, 1093
BstMWI GCNNNNNNNGC 5 cut(s) 536, 704, 749, 1136, 1145
BstNI CCWGG 3 cut(s) 54, 533, 721
BstSCI CCNGG 3 cut(s) 52, 531, 719
BstV1I GCAGC 3 cut(s) 707, 752, 1139
BstV2I GAAGAC 1 cut(s) 378
Bsu36I CCTNAGG 1 cut(s) 678
BsuI GTATCC 2 cut(s) 679, 1051
BsuRI GGCC 6 cut(s) 52, 173, 518, 530, 719, 950
BtgI CCRYGG 1 cut(s) 1079
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 3 cut(s) 430, 1089, 1192
BtsIMutI CAGTG 2 cut(s) 46, 663
BveI ACCTGC 2 cut(s) 98, 991
Cac8I GCNNGC 1 cut(s) 528
CaiI CAGNNNCTG 1 cut(s) 532
CfoI GCGC 2 cut(s) 198, 838
Cfr13I GGNCC 3 cut(s) 529, 717, 949
CseI GACGC 1 cut(s) 275
Csp6I GTAC 2 cut(s) 124, 802
CviAII CATG 3 cut(s) 206, 868, 1132
CviQI GTAC 2 cut(s) 124, 802
DdeI CTNAG 2 cut(s) 394, 678
DpnI GATC 6 cut(s) 81, 153, 675, 786, 879, 1095
DpnII GATC 6 cut(s) 79, 151, 673, 784, 877, 1093
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 6 cut(s) 225, 255, 414, 516, 588, 606
EarI CTCTTC 6 cut(s) 225, 255, 414, 516, 588, 606
EciI GGCGGA 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 990
Eco57I CTGAAG 1 cut(s) 249
Eco81I CCTNAGG 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 529
EcoRII CCWGG 3 cut(s) 52, 531, 719
EcoT14I CCWWGG 1 cut(s) 990
ErhI CCWWGG 1 cut(s) 990
FaeI CATG 3 cut(s) 209, 871, 1135
FaiI YATR 4 cut(s) 207, 869, 1113, 1133
FalI AAGNNNNNCTT 3 cut(s) 182, 214, 1182
FaqI GGGAC 1 cut(s) 1085
FatI CATG 3 cut(s) 205, 867, 1131
FbaI TGATCA 1 cut(s) 784
Fnu4HI GCNGC 3 cut(s) 696, 741, 1128
FokI GGATG 3 cut(s) 437, 1096, 1199
Fsp4HI GCNGC 3 cut(s) 696, 741, 1128
FspBI CTAG 1 cut(s) 156
GlaI GCGC 2 cut(s) 197, 837
GluI GCNGC 3 cut(s) 696, 741, 1128
GsuI CTGGAG 1 cut(s) 575
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 6 cut(s) 52, 173, 518, 530, 719, 950
HapII CCGG 1 cut(s) 957
HgaI GACGC 1 cut(s) 275
HhaI GCGC 2 cut(s) 198, 838
Hin1II CATG 3 cut(s) 209, 871, 1135
Hin6I GCGC 2 cut(s) 196, 836
HinP1I GCGC 2 cut(s) 196, 836
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HinfI GANTC 9 cut(s) 26, 377, 598, 616, 634, 641, 817, 908, 1039
HpaII CCGG 1 cut(s) 957
HphI GGTGA 5 cut(s) 449, 463, 819, 872, 1006
Hpy166II GTNNAC 2 cut(s) 477, 661
Hpy188I TCNGA 8 cut(s) 100, 321, 370, 382, 397, 603, 907, 913
Hpy188III TCNNGA 7 cut(s) 83, 143, 638, 677, 727, 788, 1006
Hpy8I GTNNAC 2 cut(s) 477, 661
Hpy99I CGWCG 2 cut(s) 104, 168
HpyAV CCTTC 7 cut(s) 101, 142, 288, 540, 697, 724, 757
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 2 cut(s) 163, 499
HpyCH4V TGCA 3 cut(s) 871, 1000, 1139
HpyF10VI GCNNNNNNNGC 5 cut(s) 536, 704, 749, 1136, 1145
HpyF3I CTNAG 2 cut(s) 394, 678
HpySE526I ACGT 2 cut(s) 163, 499
Hsp92II CATG 3 cut(s) 209, 871, 1135
HspAI GCGC 2 cut(s) 196, 836
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 6 cut(s) 79, 151, 673, 784, 877, 1093
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 4 cut(s) 98, 325, 550, 890
Lsp1109I GCAGC 3 cut(s) 707, 752, 1139
LweI GCATC 1 cut(s) 987
MaeI CTAG 1 cut(s) 156
MaeII ACGT 2 cut(s) 163, 499
MaeIII GTNAC 1 cut(s) 445
MalI GATC 6 cut(s) 81, 153, 675, 786, 879, 1095
MboI GATC 6 cut(s) 79, 151, 673, 784, 877, 1093
MhlI GDGCHC 1 cut(s) 591
MluCI AATT 2 cut(s) 18, 1009
MlyI GAGTC 2 cut(s) 902, 1033
MmeI TCCRAC 1 cut(s) 123
MroXI GAANNNNTTC 2 cut(s) 597, 615
MseI TTAA 4 cut(s) 39, 300, 861, 1201
MspI CCGG 1 cut(s) 957
MspR9I CCNGG 3 cut(s) 54, 533, 721
Mva1269I GAATGC 1 cut(s) 1141
MvaI CCWGG 3 cut(s) 54, 533, 721
MwoI GCNNNNNNNGC 5 cut(s) 536, 704, 749, 1136, 1145
NdeII GATC 6 cut(s) 79, 151, 673, 784, 877, 1093
NlaIII CATG 3 cut(s) 209, 871, 1135
NlaIV GGNNCC 1 cut(s) 951
NmeAIII GCCGAG 2 cut(s) 199, 235
PciSI GCTCTTC 1 cut(s) 516
PctI GAATGC 1 cut(s) 1141
PdmI GAANNNNTTC 2 cut(s) 597, 615
PfeI GAWTC 7 cut(s) 26, 377, 598, 616, 634, 641, 817
PflFI GACNNNGTC 1 cut(s) 162
PkrI GCNGC 3 cut(s) 697, 742, 1129
PleI GAGTC 2 cut(s) 902, 1033
PpsI GAGTC 2 cut(s) 902, 1033
Psp6I CCWGG 3 cut(s) 52, 531, 719
PspGI CCWGG 3 cut(s) 52, 531, 719
PspN4I GGNNCC 1 cut(s) 951
PspPI GGNCC 3 cut(s) 529, 717, 949
PstNI CAGNNNCTG 1 cut(s) 532
PsyI GACNNNGTC 1 cut(s) 162
RsaI GTAC 2 cut(s) 125, 803
RsaNI GTAC 2 cut(s) 124, 802
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 4 cut(s) 39, 300, 861, 1201
SatI GCNGC 3 cut(s) 696, 741, 1128
Sau3AI GATC 6 cut(s) 79, 151, 673, 784, 877, 1093
Sau96I GGNCC 3 cut(s) 529, 717, 949
ScaI AGTACT 1 cut(s) 803
SchI GAGTC 2 cut(s) 902, 1033
ScrFI CCNGG 3 cut(s) 54, 533, 721
SduI GDGCHC 1 cut(s) 591
SfaNI GCATC 1 cut(s) 987
SmlI CTYRAG 2 cut(s) 509, 725
SmoI CTYRAG 2 cut(s) 509, 725
Sse9I AATT 2 cut(s) 18, 1009
SsiI CCGC 1 cut(s) 202
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 3 cut(s) 52, 531, 719
StyI CCWWGG 1 cut(s) 990
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 2 cut(s) 166, 502
TaqI TCGA 3 cut(s) 29, 279, 591
TasI AATT 2 cut(s) 18, 1009
TatI WGTACW 2 cut(s) 123, 801
TfiI GAWTC 7 cut(s) 26, 377, 598, 616, 634, 641, 817
Tru1I TTAA 4 cut(s) 39, 300, 861, 1201
Tru9I TTAA 4 cut(s) 39, 300, 861, 1201
TscAI CASTG 2 cut(s) 53, 670
TseI GCWGC 3 cut(s) 695, 740, 1127
TspDTI ATGAA 6 cut(s) 647, 794, 856, 933, 1148, 1203
TspGWI ACGGA 1 cut(s) 890
TspRI CASTG 2 cut(s) 53, 670
Tth111I GACNNNGTC 1 cut(s) 162
XapI RAATTY 1 cut(s) 1009
XmnI GAANNNNTTC 2 cut(s) 597, 615
XspI CTAG 1 cut(s) 156
ZrmI AGTACT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.