Rh3CG257900

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0017431
Physical Location & Seq
Reverse (-)
25198327 .. 25198947
621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG257900.1

Sequence Viewer

Length: 621 bp
ATGGTGCTCAAAGAGATTCGGAAAGACGAAGAGATTCACAAGGGTTTGATGAATCAGGATTCGGCAAATGATCATGTTGACACTGACAATGATCCTGAGCAGGATACTAAAGCAGCTTGGAAGGCAAGAGAGAGGGCCAGGCTCAAGAAGGAGAGGGCATGCAGCAAGGGAAGCAGTCATTTTGAAGGAGAGAAGATGAATGATCAAGAGAGGAAGGAGTACTGGGAGAGGAAGAATCCTCCCTCACCTCCTGCGCCACAGCCCCGAAAGGGGAAGCTTAAGTTCATGCAGAGAGATCACCACAGGGGAGCTTTCTTCCGTTCGGACTCTGATGATGAATATGGCGTTTTAGGAAGAGATTATTCTGCCCCAACTGGAGAGGATAAAATGGATAAAACAAGACTGCCCAAGGTGATGCAGGTCAAGAATTTCGGGCGTAGGGGAAGGGCTAAATGGACTCATCTTGTCAATGAGGATACTACTGATTGGAACAGCCCGTGGATGTGGAATGATCGTCAAAGGTCAAGGTATAATGTGAAAATGGCAGCCATGAATGCACCAATAGCAAGACCAAAAGGAAACAAGAGATTGAGGTTCTCTGTGGAGGAAGATGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

206

Amino Acids

24.29

Weight (kDa)

9.49

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 1 - 160 3.3e-38 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017431)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479581
rosa_laevigata RLG00000023558 RLG00000029558 RLG00000029584
rosa_multiflora Rmu_sc0002232.1_g000002 Rmu_sc0007129.1_g000020
rosa_roxburghii Rroxscaffold_6G00402140
rosa_rugosa Rorug03G0176600
rosa_samantha Rh3BG260500 Rh3CG257300 Rh3CG257900
rosa_wichuraiana Rw3G020310 Rw3G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 409
AclWI GGATC 1 cut(s) 86
AcsI RAATTY 1 cut(s) 427
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 2 cut(s) 269, 270
AflII CTTAAG 1 cut(s) 278
AgsI TTSAA 1 cut(s) 185
AjnI CCWGG 1 cut(s) 137
AluBI AGCT 3 cut(s) 116, 277, 311
AluI AGCT 3 cut(s) 116, 277, 311
Alw21I GWGCWC 1 cut(s) 9
AlwI GGATC 1 cut(s) 86
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 3 cut(s) 113, 162, 545
ApoI RAATTY 1 cut(s) 427
Asp700I GAANNNNTTC 1 cut(s) 33
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 135
AsuHPI GGTGA 3 cut(s) 237, 290, 424
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 3 cut(s) 125, 174, 557
BciT130I CCWGG 1 cut(s) 139
BciVI GTATCC 2 cut(s) 97, 469
BclI TGATCA 2 cut(s) 70, 202
BfrI CTTAAG 1 cut(s) 278
BfuAI ACCTGC 1 cut(s) 409
BfuI GTATCC 2 cut(s) 97, 469
BisI GCNGC 3 cut(s) 114, 163, 546
BlsI GCNGC 3 cut(s) 115, 164, 547
BmcAI AGTACT 1 cut(s) 221
Bme1390I CCNGG 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 135
BmrFI CCNGG 1 cut(s) 139
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 1 cut(s) 405
BmuI ACTGGG 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 128
BsaJI CCNNGG 2 cut(s) 408, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 270
Bse1I ACTGG 2 cut(s) 227, 379
BseBI CCWGG 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 408, 497
BseGI GGATG 1 cut(s) 507
BseLI CCNNNNNNNGG 2 cut(s) 269, 270
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 2 cut(s) 227, 379
BseXI GCAGC 3 cut(s) 125, 174, 557
BshFI GGCC 1 cut(s) 137
BsiHKAI GWGCWC 1 cut(s) 9
BslI CCNNNNNNNGG 2 cut(s) 269, 270
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 5 cut(s) 70, 91, 202, 295, 511
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 1 cut(s) 86
BspTI CTTAAG 1 cut(s) 278
BsrI ACTGG 2 cut(s) 227, 379
BssECI CCNNGG 2 cut(s) 408, 497
BssMI GATC 5 cut(s) 70, 91, 202, 295, 511
BssT1I CCWWGG 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 139
Bst6I CTCTTC 2 cut(s) 24, 349
BstAFI CTTAAG 1 cut(s) 278
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 96
BstDSI CCRYGG 1 cut(s) 497
BstF5I GGATG 1 cut(s) 507
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 5 cut(s) 73, 94, 205, 298, 514
BstMBI GATC 5 cut(s) 70, 91, 202, 295, 511
BstMWI GCNNNNNNNGC 4 cut(s) 122, 171, 554, 563
BstNI CCWGG 1 cut(s) 139
BstNSI RCATGY 1 cut(s) 162
BstSCI CCNGG 1 cut(s) 137
BstV1I GCAGC 3 cut(s) 125, 174, 557
BsuI GTATCC 2 cut(s) 97, 469
BsuRI GGCC 1 cut(s) 137
BtgI CCRYGG 1 cut(s) 497
BtsCI GGATG 1 cut(s) 507
BtsIMutI CAGTG 1 cut(s) 81
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 160
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 220
CviAII CATG 4 cut(s) 74, 159, 286, 550
CviJI RGCY 9 cut(s) 116, 137, 142, 262, 277, 311, 449, 495, 548
CviKI_1 RGCY 9 cut(s) 116, 137, 142, 262, 277, 311, 449, 495, 548
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 1 cut(s) 96
DpnI GATC 5 cut(s) 72, 93, 204, 297, 513
DpnII GATC 5 cut(s) 70, 91, 202, 295, 511
Eam1104I CTCTTC 2 cut(s) 24, 349
EarI CTCTTC 2 cut(s) 24, 349
Eco130I CCWWGG 1 cut(s) 408
EcoRII CCWGG 1 cut(s) 137
EcoT14I CCWWGG 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 408
FaeI CATG 4 cut(s) 77, 162, 289, 553
FaiI YATR 6 cut(s) 75, 160, 287, 342, 531, 551
FalI AAGNNNNNCTT 1 cut(s) 600
FatI CATG 4 cut(s) 73, 158, 285, 549
FbaI TGATCA 2 cut(s) 70, 202
Fnu4HI GCNGC 3 cut(s) 114, 163, 546
FokI GGATG 1 cut(s) 514
Fsp4HI GCNGC 3 cut(s) 114, 163, 546
GlaI GCGC 1 cut(s) 255
GluI GCNGC 3 cut(s) 114, 163, 546
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 1 cut(s) 137
HhaI GCGC 1 cut(s) 256
Hin1II CATG 4 cut(s) 77, 162, 289, 553
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 7 cut(s) 16, 34, 52, 59, 235, 326, 457
HphI GGTGA 3 cut(s) 237, 290, 424
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 3 cut(s) 21, 325, 331
Hpy188III TCNNGA 5 cut(s) 56, 95, 145, 206, 424
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 5 cut(s) 115, 142, 179, 208, 438
HpyCH4V TGCA 4 cut(s) 162, 289, 418, 557
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 171, 554, 563
HpyF3I CTNAG 1 cut(s) 96
Hsp92II CATG 4 cut(s) 77, 162, 289, 553
HspAI GCGC 1 cut(s) 254
Ksp22I TGATCA 2 cut(s) 70, 202
Kzo9I GATC 5 cut(s) 70, 91, 202, 295, 511
LmnI GCTCC 1 cut(s) 308
Lsp1109I GCAGC 3 cut(s) 125, 174, 557
LweI GCATC 1 cut(s) 405
MalI GATC 5 cut(s) 72, 93, 204, 297, 513
MboI GATC 5 cut(s) 70, 91, 202, 295, 511
MboII GAAGA 6 cut(s) 41, 205, 244, 307, 366, 620
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 1 cut(s) 427
MlyI GAGTC 2 cut(s) 320, 451
MroXI GAANNNNTTC 1 cut(s) 33
MseI TTAA 2 cut(s) 279, 619
MspCI CTTAAG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 139
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 1 cut(s) 139
MwoI GCNNNNNNNGC 4 cut(s) 122, 171, 554, 563
NdeII GATC 5 cut(s) 70, 91, 202, 295, 511
NlaIII CATG 4 cut(s) 77, 162, 289, 553
NspI RCATGY 1 cut(s) 162
PaeI GCATGC 1 cut(s) 162
PctI GAATGC 1 cut(s) 559
PdmI GAANNNNTTC 1 cut(s) 33
PfeI GAWTC 5 cut(s) 16, 34, 52, 59, 235
PkrI GCNGC 3 cut(s) 115, 164, 547
PleI GAGTC 2 cut(s) 320, 451
PpsI GAGTC 2 cut(s) 320, 451
Psp6I CCWGG 1 cut(s) 137
PspGI CCWGG 1 cut(s) 137
PspPI GGNCC 1 cut(s) 135
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SaqAI TTAA 2 cut(s) 279, 619
SatI GCNGC 3 cut(s) 114, 163, 546
Sau3AI GATC 5 cut(s) 70, 91, 202, 295, 511
Sau96I GGNCC 1 cut(s) 135
ScaI AGTACT 1 cut(s) 221
SchI GAGTC 2 cut(s) 320, 451
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 1 cut(s) 9
SetI ASST 9 cut(s) 118, 250, 279, 313, 414, 423, 524, 530, 596
SfaNI GCATC 1 cut(s) 405
SmlI CTYRAG 2 cut(s) 143, 278
SmoI CTYRAG 2 cut(s) 143, 278
SphI GCATGC 1 cut(s) 162
Sse9I AATT 1 cut(s) 427
StyD4I CCNGG 1 cut(s) 137
StyI CCWWGG 1 cut(s) 408
TasI AATT 1 cut(s) 427
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 5 cut(s) 16, 34, 52, 59, 235
Tru1I TTAA 2 cut(s) 279, 619
Tru9I TTAA 2 cut(s) 279, 619
TscAI CASTG 1 cut(s) 88
TseI GCWGC 3 cut(s) 113, 162, 545
TspDTI ATGAA 5 cut(s) 65, 212, 274, 351, 566
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 1 cut(s) 88
Vha464I CTTAAG 1 cut(s) 278
XapI RAATTY 1 cut(s) 427
XceI RCATGY 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 33
ZrmI AGTACT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.