Rh3CG257300

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0017431
Physical Location & Seq
Reverse (-)
25100567 .. 25101142
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG257300.1

Sequence Viewer

Length: 576 bp
ATGAATCAGGATTCGGCAAATGATGATGTTGACACTGACATTAATGATCCTGAGGAGGATACTAAAGCAGCTTGGAAGGCAAGAGAGAGGGCCAGGCTCAAGAAGGAGAGGGCAGCAAGGGAAGTAGTCATTTTGAAGGAGATAGAGAAGATGAATGATCAAGTGAGGAGGGAGTACTGGGAGAGGAAGAATCCTCCCTCATCTCCTGCGCCACAGCCCCGAAAGAGGAAGCTTAAGTTCATGCAGAGAGATTACCACAGGGGAGCTTTCTTCCGTTCGGACCCTGATGATGAAGATGGCGTTTTAGGAAGAGATTATTCTACCCCAACCGGAGAGGATAAGATGGATAAAACAAGACTGCCCAAGGTGATGCAGGTCAAGAATTTCGGGCGTAGGGGAAGGGCTAAATGGACTCATCTTGTCAATGAGGATACTACTGATTGGAACAGCCCGTGGATGTGGAATGATCGTCAAAGGTCAGGGTATAATGTGAAAATGGCAGCCATGAATGCACCAATAGCAAGACCAAAAGGAAACAAGAGATTGAGGTTCTCTGTGAAGGAAGATGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

191

Amino Acids

22.59

Weight (kDa)

9.63

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 4 - 145 7.7e-38 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017431)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479581
rosa_laevigata RLG00000023558 RLG00000029558 RLG00000029584
rosa_multiflora Rmu_sc0002232.1_g000002 Rmu_sc0007129.1_g000020
rosa_roxburghii Rroxscaffold_6G00402140
rosa_rugosa Rorug03G0176600
rosa_samantha Rh3BG260500 Rh3CG257300 Rh3CG257900
rosa_wichuraiana Rw3G020310 Rw3G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 364
AclWI GGATC 1 cut(s) 41
AcsI RAATTY 1 cut(s) 382
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 1 cut(s) 225
AflII CTTAAG 1 cut(s) 233
AgsI TTSAA 1 cut(s) 136
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 3 cut(s) 71, 232, 266
AluI AGCT 3 cut(s) 71, 232, 266
AlwI GGATC 1 cut(s) 41
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 3 cut(s) 68, 113, 500
ApoI RAATTY 1 cut(s) 382
AseI ATTAAT 1 cut(s) 42
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 2 cut(s) 90, 280
AsuHPI GGTGA 1 cut(s) 379
AvaII GGWCC 1 cut(s) 280
AxyI CCTNAGG 1 cut(s) 51
BbvI GCAGC 3 cut(s) 80, 125, 512
BccI CCATC 2 cut(s) 290, 337
BciT130I CCWGG 1 cut(s) 94
BciVI GTATCC 2 cut(s) 52, 424
BclI TGATCA 1 cut(s) 157
BfrI CTTAAG 1 cut(s) 233
BfuAI ACCTGC 1 cut(s) 364
BfuI GTATCC 2 cut(s) 52, 424
BisI GCNGC 3 cut(s) 69, 114, 501
BlsI GCNGC 3 cut(s) 70, 115, 502
BmcAI AGTACT 1 cut(s) 176
Bme1390I CCNGG 1 cut(s) 94
Bme18I GGWCC 1 cut(s) 280
BmgT120I GGNCC 2 cut(s) 90, 280
BmiI GGNNCC 1 cut(s) 282
BmrFI CCNGG 1 cut(s) 94
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 1 cut(s) 360
BmuI ACTGGG 1 cut(s) 187
BpuEI CTTGAG 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 363, 452
BsaWI WCCGGW 1 cut(s) 329
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse1I ACTGG 1 cut(s) 182
Bse21I CCTNAGG 1 cut(s) 51
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 2 cut(s) 363, 452
BseGI GGATG 1 cut(s) 462
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 1 cut(s) 182
BseRI GAGGAG 2 cut(s) 68, 181
BseXI GCAGC 3 cut(s) 80, 125, 512
BshFI GGCC 1 cut(s) 92
BsiSI CCGG 1 cut(s) 330
BslI CCNNNNNNNGG 1 cut(s) 225
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 1 cut(s) 92
Bsp143I GATC 3 cut(s) 46, 157, 466
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 1 cut(s) 43
BspLI GGNNCC 1 cut(s) 282
BspMI ACCTGC 1 cut(s) 364
BspPI GGATC 1 cut(s) 41
BspTI CTTAAG 1 cut(s) 233
BsrI ACTGG 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 363, 452
BssMI GATC 3 cut(s) 46, 157, 466
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 304
BstAFI CTTAAG 1 cut(s) 233
BstDEI CTNAG 1 cut(s) 51
BstDSI CCRYGG 1 cut(s) 452
BstF5I GGATG 1 cut(s) 462
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 3 cut(s) 49, 160, 469
BstMBI GATC 3 cut(s) 46, 157, 466
BstMWI GCNNNNNNNGC 3 cut(s) 77, 509, 518
BstNI CCWGG 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 92
BstV1I GCAGC 3 cut(s) 80, 125, 512
Bsu36I CCTNAGG 1 cut(s) 51
BsuI GTATCC 2 cut(s) 52, 424
BsuRI GGCC 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 452
BtsCI GGATG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 33
BveI ACCTGC 1 cut(s) 364
CfoI GCGC 1 cut(s) 211
Cfr13I GGNCC 2 cut(s) 90, 280
Csp6I GTAC 1 cut(s) 175
CviAII CATG 2 cut(s) 241, 505
CviJI RGCY 9 cut(s) 71, 92, 97, 217, 232, 266, 404, 450, 503
CviKI_1 RGCY 9 cut(s) 71, 92, 97, 217, 232, 266, 404, 450, 503
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 1 cut(s) 51
DpnI GATC 3 cut(s) 48, 159, 468
DpnII GATC 3 cut(s) 46, 157, 466
Eam1104I CTCTTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 280
Eco81I CCTNAGG 1 cut(s) 51
EcoRII CCWGG 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 2 cut(s) 244, 508
FaiI YATR 3 cut(s) 242, 486, 506
FalI AAGNNNNNCTT 1 cut(s) 555
FatI CATG 2 cut(s) 240, 504
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 3 cut(s) 69, 114, 501
FokI GGATG 1 cut(s) 469
Fsp4HI GCNGC 3 cut(s) 69, 114, 501
GlaI GCGC 1 cut(s) 210
GluI GCNGC 3 cut(s) 69, 114, 501
HaeIII GGCC 1 cut(s) 92
HapII CCGG 1 cut(s) 330
HhaI GCGC 1 cut(s) 211
Hin1II CATG 2 cut(s) 244, 508
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 4 cut(s) 4, 11, 190, 412
HpaII CCGG 1 cut(s) 330
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 1 cut(s) 280
Hpy188III TCNNGA 4 cut(s) 8, 50, 100, 379
Hpy8I GTNNAC 1 cut(s) 31
HpyAV CCTTC 5 cut(s) 70, 97, 130, 393, 553
HpyCH4V TGCA 3 cut(s) 244, 373, 512
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 509, 518
HpyF3I CTNAG 1 cut(s) 51
Hsp92II CATG 2 cut(s) 244, 508
HspAI GCGC 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 3 cut(s) 46, 157, 466
LmnI GCTCC 1 cut(s) 263
Lsp1109I GCAGC 3 cut(s) 80, 125, 512
LweI GCATC 1 cut(s) 360
MalI GATC 3 cut(s) 48, 159, 468
MboI GATC 3 cut(s) 46, 157, 466
MboII GAAGA 6 cut(s) 160, 199, 262, 305, 321, 575
MluCI AATT 1 cut(s) 382
MlyI GAGTC 1 cut(s) 406
MseI TTAA 3 cut(s) 42, 234, 574
MspCI CTTAAG 1 cut(s) 233
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 1 cut(s) 94
Mva1269I GAATGC 1 cut(s) 514
MvaI CCWGG 1 cut(s) 94
MwoI GCNNNNNNNGC 3 cut(s) 77, 509, 518
NdeII GATC 3 cut(s) 46, 157, 466
NlaIII CATG 2 cut(s) 244, 508
NlaIV GGNNCC 1 cut(s) 282
PctI GAATGC 1 cut(s) 514
PfeI GAWTC 3 cut(s) 4, 11, 190
PkrI GCNGC 3 cut(s) 70, 115, 502
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
PshBI ATTAAT 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 92
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 1 cut(s) 282
PspPI GGNCC 2 cut(s) 90, 280
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SaqAI TTAA 3 cut(s) 42, 234, 574
SatI GCNGC 3 cut(s) 69, 114, 501
Sau3AI GATC 3 cut(s) 46, 157, 466
Sau96I GGNCC 2 cut(s) 90, 280
ScaI AGTACT 1 cut(s) 176
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 1 cut(s) 94
SetI ASST 7 cut(s) 73, 234, 268, 369, 378, 479, 551
SfaNI GCATC 1 cut(s) 360
SinI GGWCC 1 cut(s) 280
SmlI CTYRAG 2 cut(s) 98, 233
SmoI CTYRAG 2 cut(s) 98, 233
Sse9I AATT 1 cut(s) 382
StyD4I CCNGG 1 cut(s) 92
StyI CCWWGG 1 cut(s) 363
TasI AATT 1 cut(s) 382
TatI WGTACW 1 cut(s) 174
TfiI GAWTC 3 cut(s) 4, 11, 190
Tru1I TTAA 3 cut(s) 42, 234, 574
Tru9I TTAA 3 cut(s) 42, 234, 574
TscAI CASTG 1 cut(s) 40
TseI GCWGC 3 cut(s) 68, 113, 500
TspDTI ATGAA 5 cut(s) 17, 167, 229, 306, 521
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 1 cut(s) 40
Vha464I CTTAAG 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 280
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 1 cut(s) 382
ZrmI AGTACT 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.