RLG00000029558

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017431
Physical Location & Seq
Reverse (-)
40359445 .. 40359988
544 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029558

Sequence Viewer

Length: 453 bp
ATGGTGCTCGAAGAGATTTGGAAAGACGAAGAGATTCACAAGGGTTTGATGAATCAGGATTCGGTAAATGATGATGTCGACATTGACGATAATCTTGAGGAGGATATTAAAGCTTCGAGGGCAAGAGAGAGGGCGAGGCTCAAAAAGGAGAGGGAAGCAAAAAAAGCAATCATTATGAAGGAGATAGAGATTGAGAGACTGAAGAAGAGAGATGATCACAGGGGAGTTTTCTTCCTTTCAGACTCTGATGATGAAGATGGCGTTTTAGGAAAAGATGATTCTGCCCCAACCCGAGAGGATAAGATGGATAAAACAAGACTGTCTAAGGTGATGCAGGTCAAGAATTTCGGGCATACAGGAATGGCTAAATGGACTCATCTTGTCAATGAGGACACACTAATTGGAACAACCCATGGATGTGGAATAATTGTCAAAGATCAAGGTATAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

151

Amino Acids

17.25

Weight (kDa)

5.19

Isoelectric Point (pI)

32.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 73 - 132 1.5e-16 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017431)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479581
rosa_laevigata RLG00000023558 RLG00000029558 RLG00000029584
rosa_multiflora Rmu_sc0002232.1_g000002 Rmu_sc0007129.1_g000020
rosa_roxburghii Rroxscaffold_6G00402140
rosa_rugosa Rorug03G0176600
rosa_samantha Rh3BG260500 Rh3CG257300 Rh3CG257900
rosa_wichuraiana Rw3G020310 Rw3G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 325
AccI GTMKAC 1 cut(s) 78
AcsI RAATTY 1 cut(s) 343
AcuI CTGAAG 1 cut(s) 221
AluBI AGCT 1 cut(s) 113
AluI AGCT 1 cut(s) 113
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 190
AlwNI CAGNNNCTG 1 cut(s) 245
Ama87I CYCGRG 1 cut(s) 291
ApoI RAATTY 1 cut(s) 343
Asp700I GAANNNNTTC 1 cut(s) 33
AsuHPI GGTGA 1 cut(s) 340
AvaI CYCGRG 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 9
BccI CCATC 2 cut(s) 251, 298
BclI TGATCA 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 291
BmsI GCATC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 412
BseDI CCNNGG 1 cut(s) 412
BseGI GGATG 1 cut(s) 422
BseRI GAGGAG 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 291
BsmAI GTCTC 1 cut(s) 190
BsoBI CYCGRG 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 2 cut(s) 214, 436
Bsp19I CCATGG 1 cut(s) 412
BspMI ACCTGC 1 cut(s) 325
BssECI CCNNGG 1 cut(s) 412
BssMI GATC 2 cut(s) 214, 436
BssT1I CCWWGG 1 cut(s) 412
Bst4CI ACNGT 1 cut(s) 321
Bst6I CTCTTC 3 cut(s) 6, 24, 200
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 412
BstF5I GGATG 1 cut(s) 422
BstKTI GATC 2 cut(s) 217, 439
BstMAI GTCTC 1 cut(s) 190
BstMBI GATC 2 cut(s) 214, 436
BstMWI GCNNNNNNNGC 2 cut(s) 119, 164
BstXI CCANNNNNNTGG 1 cut(s) 419
BtgI CCRYGG 1 cut(s) 412
BtsCI GGATG 1 cut(s) 422
BveI ACCTGC 1 cut(s) 325
CaiI CAGNNNCTG 1 cut(s) 245
CviAII CATG 1 cut(s) 413
CviJI RGCY 3 cut(s) 113, 139, 365
CviKI_1 RGCY 3 cut(s) 113, 139, 365
DdeI CTNAG 1 cut(s) 324
DpnI GATC 2 cut(s) 216, 438
DpnII GATC 2 cut(s) 214, 436
Eam1104I CTCTTC 3 cut(s) 6, 24, 200
EarI CTCTTC 3 cut(s) 6, 24, 200
Eco130I CCWWGG 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 221
Eco88I CYCGRG 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 412
FaeI CATG 1 cut(s) 416
FaiI YATR 4 cut(s) 176, 354, 414, 446
FatI CATG 1 cut(s) 412
FbaI TGATCA 1 cut(s) 214
FblI GTMKAC 1 cut(s) 78
FokI GGATG 1 cut(s) 429
Hin1II CATG 1 cut(s) 416
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 1 cut(s) 111
HinfI GANTC 6 cut(s) 34, 52, 59, 242, 278, 373
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 2 cut(s) 241, 247
Hpy188III TCNNGA 3 cut(s) 56, 95, 340
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 1 cut(s) 172
HpyCH4III ACNGT 1 cut(s) 321
HpyCH4V TGCA 1 cut(s) 334
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 164
HpyF3I CTNAG 1 cut(s) 324
Hsp92II CATG 1 cut(s) 416
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 2 cut(s) 214, 436
LpnPI CCDG 4 cut(s) 41, 205, 320, 342
LweI GCATC 1 cut(s) 321
MalI GATC 2 cut(s) 216, 438
MboI GATC 2 cut(s) 214, 436
MboII GAAGA 6 cut(s) 23, 41, 214, 217, 223, 266
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 3 cut(s) 343, 399, 426
MlyI GAGTC 2 cut(s) 236, 367
MnlI CCTC 8 cut(s) 91, 94, 111, 123, 129, 144, 289, 382
MroXI GAANNNNTTC 1 cut(s) 33
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 1 cut(s) 417
MwoI GCNNNNNNNGC 2 cut(s) 119, 164
NcoI CCATGG 1 cut(s) 412
NdeII GATC 2 cut(s) 214, 436
NlaIII CATG 1 cut(s) 416
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PdmI GAANNNNTTC 1 cut(s) 33
PfeI GAWTC 4 cut(s) 34, 52, 59, 278
PleI GAGTC 2 cut(s) 236, 367
PpsI GAGTC 2 cut(s) 236, 367
PstNI CAGNNNCTG 1 cut(s) 245
RseI CAYNNNNRTG 1 cut(s) 417
SalI GTCGAC 1 cut(s) 77
SaqAI TTAA 1 cut(s) 108
Sau3AI GATC 2 cut(s) 214, 436
SchI GAGTC 2 cut(s) 236, 367
SduI GDGCHC 1 cut(s) 9
SetI ASST 4 cut(s) 115, 330, 339, 445
SfaNI GCATC 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 3 cut(s) 343, 399, 426
StyI CCWWGG 1 cut(s) 412
TaaI ACNGT 1 cut(s) 321
TaqI TCGA 3 cut(s) 9, 78, 116
TasI AATT 3 cut(s) 343, 399, 426
TfiI GAWTC 4 cut(s) 34, 52, 59, 278
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TspDTI ATGAA 3 cut(s) 65, 191, 267
XapI RAATTY 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.