Rw3G020360

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0017431
Physical Location & Seq
Reverse (-)
23841523 .. 23842717
1195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G020360.1

Sequence Viewer

Length: 1140 bp
ATGGCGATGCGAGGCAAAATTAGGGAATCGACAAAAGTTAAAAGATACTGGCCTGGAAAAGCCCCAGAGTGGGCAAACGATCGTGATACCTGCTCCTCCGACGAAGAAGGGATTACCATCTTGTACAAATCCTTCTCAACTCGTGATGATGATCCAAGACTACGTCGTTTGGCCGAGAGGAGGAGTGTAAAGAGGCGCATCCGCGGTGCCAAGATTGTTGACACAACTGAAGAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGAGGACGCAGAGACTTTGGAGGAAAGAAGAAGAAGGATTAAGGAGAGGTTGCTTCATGAATCCGATGAGGACTACGAAGAAGAAGAAGAAGAAGAGGAGGATGAGATTGCTGGTGAAGTAGTTACAGTGAAGCCTGTATTTTTGTCCAAGTCCACAAGAGACACGATAGTTGAACGTAAGAGACTTGAGGAGGCCGAAGAGCAGGCCCTGGAAGCGAGGAGAAGGGAGCTGGAGGAGAGGAAGAAAGAGACAAGGAGAATGGTGCTCAAAGAGATTCGGAAAGACGAAGAGATTCACAAGGGTTTGATGAATCAGGATTCGGCAAATGATCATGTTGACACTGACAATGATCCTGAGCAGGATACTAAAGCAGCTTGGAAGGCAAGAGAGAGGGCCAGGCTCAAGAAGGAGAGGGCATGCAGCAAGGGAAGCAGTCATTTTGAAGGAGAGAAGATGAATGATCAAGAGAGGAAGGAGTACTGGGAGAGGAAGAATCCTCCCTCACCTCCTGCGCCACAGCCCCGAAAGGGGAAGCTTAAGTTCATGCAGAGAGATCACCACAGGGGAGCTTTCTTCCGTTCGGACTCTGATGATGAATATGGCGTTTTAGGAAGAGATTATTCTGCCCCAACTGGAGAGGATAAAATGGATAAAACAAGACTGCCCAAGGTGATGCAGGTCAAGAATTTCGGGCGTAGGGGAAGGGCTAAATGGACTCATCTTGTCAATGAGGATACTACTGATTGGAACAGCCCGTGGATGTGGAATGATCGTCAAAGGTCAAGGTATAATGTGAAAATGGCAGCCATGAATGCACCAATAGCAAGACCAAAAGGAAACAAGAGATTGAGGTTCTCTGTGGAGGAAGATGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

379

Amino Acids

45.11

Weight (kDa)

5.82

Isoelectric Point (pI)

67.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 119 - 333 3.9e-49 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017431)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479581
rosa_laevigata RLG00000023558 RLG00000029558 RLG00000029584
rosa_multiflora Rmu_sc0002232.1_g000002 Rmu_sc0007129.1_g000020
rosa_roxburghii Rroxscaffold_6G00402140
rosa_rugosa Rorug03G0176600
rosa_samantha Rh3BG260500 Rh3CG257300 Rh3CG257900
rosa_wichuraiana Rw3G020310 Rw3G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 98, 928
AccB1I GGYRCC 1 cut(s) 206
AccII CGCG 1 cut(s) 204
AciI CCGC 2 cut(s) 202, 204
AclWI GGATC 2 cut(s) 146, 605
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 946
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 2 cut(s) 125, 740
AfiI CCNNNNNNNGG 5 cut(s) 69, 70, 180, 788, 789
AflII CTTAAG 1 cut(s) 797
AgsI TTSAA 2 cut(s) 434, 704
AjnI CCWGG 3 cut(s) 52, 468, 656
AluBI AGCT 4 cut(s) 490, 635, 796, 830
AluI AGCT 4 cut(s) 490, 635, 796, 830
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 4 cut(s) 266, 414, 436, 503
AlwI GGATC 2 cut(s) 146, 605
AlwNI CAGNNNCTG 1 cut(s) 469
AoxI GGCC 5 cut(s) 50, 171, 453, 465, 654
ApeKI GCWGC 3 cut(s) 632, 681, 1064
ApoI RAATTY 1 cut(s) 946
Asp700I GAANNNNTTC 1 cut(s) 552
AspLEI GCGC 2 cut(s) 198, 775
AspS9I GGNCC 2 cut(s) 466, 654
AsuHPI GGTGA 4 cut(s) 386, 756, 809, 943
BanI GGYRCC 1 cut(s) 206
BarI GAAGNNNNNNTAC 2 cut(s) 116, 148
BauI CACGAG 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 3 cut(s) 644, 693, 1076
BccI CCATC 1 cut(s) 125
BciT130I CCWGG 3 cut(s) 54, 470, 658
BciVI GTATCC 2 cut(s) 616, 988
BclI TGATCA 2 cut(s) 589, 721
BcoDI GTCTC 4 cut(s) 266, 414, 436, 503
BfrI CTTAAG 1 cut(s) 797
BfuAI ACCTGC 2 cut(s) 98, 928
BfuI GTATCC 2 cut(s) 616, 988
BisI GCNGC 3 cut(s) 633, 682, 1065
BlsI GCNGC 3 cut(s) 634, 683, 1066
BmcAI AGTACT 1 cut(s) 740
Bme1390I CCNGG 3 cut(s) 54, 470, 658
BmgT120I GGNCC 2 cut(s) 466, 654
BmiI GGNNCC 1 cut(s) 208
BmrFI CCNGG 3 cut(s) 54, 470, 658
BmrI ACTGGG 1 cut(s) 751
BmsI GCATC 2 cut(s) 207, 924
BmuI ACTGGG 1 cut(s) 751
BpmI CTGGAG 2 cut(s) 512, 915
Bpu10I CCTNAGC 1 cut(s) 615
BpuEI CTTGAG 2 cut(s) 467, 647
BsaBI GATNNNNATC 2 cut(s) 116, 150
BsaJI CCNNGG 4 cut(s) 202, 468, 927, 1016
Bsc4I CCNNNNNNNGG 5 cut(s) 69, 70, 180, 788, 789
Bse1I ACTGG 3 cut(s) 53, 746, 898
Bse8I GATNNNNATC 2 cut(s) 116, 150
BseBI CCWGG 3 cut(s) 54, 470, 658
BseDI CCNNGG 4 cut(s) 202, 468, 927, 1016
BseGI GGATG 3 cut(s) 198, 367, 1026
BseJI GATNNNNATC 2 cut(s) 116, 150
BseLI CCNNNNNNNGG 5 cut(s) 69, 70, 180, 788, 789
BseMII CTCAG 1 cut(s) 606
BseNI ACTGG 3 cut(s) 53, 746, 898
BseXI GCAGC 3 cut(s) 644, 693, 1076
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 5 cut(s) 52, 173, 455, 467, 656
BshNI GGYRCC 1 cut(s) 206
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 528
BslI CCNNNNNNNGG 5 cut(s) 69, 70, 180, 788, 789
BsmAI GTCTC 4 cut(s) 266, 414, 436, 503
BsmI GAATGC 1 cut(s) 1078
BsnI GGCC 5 cut(s) 52, 173, 455, 467, 656
Bsp1286I GDGCHC 1 cut(s) 528
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 7 cut(s) 79, 151, 589, 610, 721, 814, 1030
BspACI CCGC 2 cut(s) 202, 204
BspANI GGCC 5 cut(s) 52, 173, 455, 467, 656
BspCNI CTCAG 1 cut(s) 607
BspFNI CGCG 1 cut(s) 204
BspHI TCATGA 1 cut(s) 316
BspLI GGNNCC 1 cut(s) 208
BspMI ACCTGC 2 cut(s) 98, 928
BspPI GGATC 2 cut(s) 146, 605
BspQI GCTCTTC 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 206
BspTI CTTAAG 1 cut(s) 797
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 3 cut(s) 53, 746, 898
BssECI CCNNGG 4 cut(s) 202, 468, 927, 1016
BssMI GATC 7 cut(s) 79, 151, 589, 610, 721, 814, 1030
BssSI CACGAG 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 927
Bst2BI CACGAG 1 cut(s) 141
Bst2UI CCWGG 3 cut(s) 54, 470, 658
Bst4CI ACNGT 1 cut(s) 388
Bst6I CTCTTC 6 cut(s) 225, 255, 348, 453, 543, 868
BstAFI CTTAAG 1 cut(s) 797
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 465, 679
BstDEI CTNAG 1 cut(s) 615
BstDSI CCRYGG 2 cut(s) 202, 1016
BstF5I GGATG 3 cut(s) 198, 367, 1026
BstFNI CGCG 1 cut(s) 204
BstHHI GCGC 2 cut(s) 198, 775
BstKTI GATC 7 cut(s) 82, 154, 592, 613, 724, 817, 1033
BstMAI GTCTC 4 cut(s) 266, 414, 436, 503
BstMBI GATC 7 cut(s) 79, 151, 589, 610, 721, 814, 1030
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 5 cut(s) 473, 641, 690, 1073, 1082
BstNI CCWGG 3 cut(s) 54, 470, 658
BstNSI RCATGY 1 cut(s) 681
BstSCI CCNGG 3 cut(s) 52, 468, 656
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 3 cut(s) 644, 693, 1076
BsuI GTATCC 2 cut(s) 616, 988
BsuRI GGCC 5 cut(s) 52, 173, 455, 467, 656
BtgI CCRYGG 2 cut(s) 202, 1016
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 3 cut(s) 198, 367, 1026
BtsIMutI CAGTG 2 cut(s) 393, 600
BveI ACCTGC 2 cut(s) 98, 928
Cac8I GCNNGC 2 cut(s) 465, 679
CaiI CAGNNNCTG 1 cut(s) 469
CciI TCATGA 1 cut(s) 316
CfoI GCGC 2 cut(s) 198, 775
Cfr13I GGNCC 2 cut(s) 466, 654
Cfr42I CCGCGG 1 cut(s) 205
CseI GACGC 1 cut(s) 275
Csp6I GTAC 2 cut(s) 124, 739
CviAII CATG 5 cut(s) 317, 593, 678, 805, 1069
CviQI GTAC 2 cut(s) 124, 739
DdeI CTNAG 1 cut(s) 615
DpnI GATC 7 cut(s) 81, 153, 591, 612, 723, 816, 1032
DpnII GATC 7 cut(s) 79, 151, 589, 610, 721, 814, 1030
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 6 cut(s) 225, 255, 348, 453, 543, 868
EarI CTCTTC 6 cut(s) 225, 255, 348, 453, 543, 868
Eco130I CCWWGG 1 cut(s) 927
Eco57I CTGAAG 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 466
EcoRII CCWGG 3 cut(s) 52, 468, 656
EcoT14I CCWWGG 1 cut(s) 927
ErhI CCWWGG 1 cut(s) 927
FaeI CATG 5 cut(s) 320, 596, 681, 808, 1072
FaiI YATR 7 cut(s) 318, 594, 679, 806, 861, 1050, 1070
FalI AAGNNNNNCTT 1 cut(s) 1119
FatI CATG 5 cut(s) 316, 592, 677, 804, 1068
FbaI TGATCA 2 cut(s) 589, 721
Fnu4HI GCNGC 3 cut(s) 633, 682, 1065
FokI GGATG 3 cut(s) 185, 374, 1033
Fsp4HI GCNGC 3 cut(s) 633, 682, 1065
GlaI GCGC 2 cut(s) 197, 774
GluI GCNGC 3 cut(s) 633, 682, 1065
GsuI CTGGAG 2 cut(s) 512, 915
HaeIII GGCC 5 cut(s) 52, 173, 455, 467, 656
HgaI GACGC 1 cut(s) 275
HhaI GCGC 2 cut(s) 198, 775
Hin1II CATG 5 cut(s) 320, 596, 681, 808, 1072
Hin6I GCGC 2 cut(s) 196, 773
HinP1I GCGC 2 cut(s) 196, 773
HincII GTYRAC 2 cut(s) 220, 598
HindII GTYRAC 2 cut(s) 220, 598
HindIII AAGCTT 1 cut(s) 794
HinfI GANTC 9 cut(s) 26, 320, 535, 553, 571, 578, 754, 845, 976
HphI GGTGA 4 cut(s) 386, 756, 809, 943
Hpy166II GTNNAC 3 cut(s) 220, 414, 598
Hpy188I TCNGA 5 cut(s) 100, 325, 540, 844, 850
Hpy188III TCNNGA 8 cut(s) 83, 143, 317, 575, 614, 664, 725, 943
Hpy8I GTNNAC 3 cut(s) 220, 414, 598
Hpy99I CGWCG 2 cut(s) 104, 168
HpyAV CCTTC 9 cut(s) 101, 142, 288, 477, 634, 661, 698, 727, 957
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4IV ACGT 2 cut(s) 163, 436
HpyCH4V TGCA 4 cut(s) 681, 808, 937, 1076
HpyF10VI GCNNNNNNNGC 5 cut(s) 473, 641, 690, 1073, 1082
HpyF3I CTNAG 1 cut(s) 615
HpySE526I ACGT 2 cut(s) 163, 436
Hsp92II CATG 5 cut(s) 320, 596, 681, 808, 1072
HspAI GCGC 2 cut(s) 196, 773
Ksp22I TGATCA 2 cut(s) 589, 721
KspI CCGCGG 1 cut(s) 205
Kzo9I GATC 7 cut(s) 79, 151, 589, 610, 721, 814, 1030
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 3 cut(s) 98, 487, 827
Lsp1109I GCAGC 3 cut(s) 644, 693, 1076
LweI GCATC 2 cut(s) 207, 924
MaeII ACGT 2 cut(s) 163, 436
MaeIII GTNAC 1 cut(s) 382
MalI GATC 7 cut(s) 81, 153, 591, 612, 723, 816, 1032
MboI GATC 7 cut(s) 79, 151, 589, 610, 721, 814, 1030
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 2 cut(s) 18, 946
MlyI GAGTC 2 cut(s) 839, 970
MmeI TCCRAC 1 cut(s) 123
MroXI GAANNNNTTC 1 cut(s) 552
MseI TTAA 4 cut(s) 39, 300, 798, 1138
MspA1I CMGCKG 1 cut(s) 204
MspCI CTTAAG 1 cut(s) 797
MspR9I CCNGG 3 cut(s) 54, 470, 658
Mva1269I GAATGC 1 cut(s) 1078
MvaI CCWGG 3 cut(s) 54, 470, 658
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 5 cut(s) 473, 641, 690, 1073, 1082
NdeII GATC 7 cut(s) 79, 151, 589, 610, 721, 814, 1030
NlaIII CATG 5 cut(s) 320, 596, 681, 808, 1072
NlaIV GGNNCC 1 cut(s) 208
NmeAIII GCCGAG 1 cut(s) 199
NspI RCATGY 1 cut(s) 681
PaeI GCATGC 1 cut(s) 681
PagI TCATGA 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 453
PctI GAATGC 1 cut(s) 1078
PdmI GAANNNNTTC 1 cut(s) 552
PfeI GAWTC 7 cut(s) 26, 320, 535, 553, 571, 578, 754
PflFI GACNNNGTC 1 cut(s) 162
PkrI GCNGC 3 cut(s) 634, 683, 1066
Ple19I CGATCG 1 cut(s) 82
PleI GAGTC 2 cut(s) 839, 970
PpsI GAGTC 2 cut(s) 839, 970
Psp6I CCWGG 3 cut(s) 52, 468, 656
PspGI CCWGG 3 cut(s) 52, 468, 656
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 466, 654
PstNI CAGNNNCTG 1 cut(s) 469
PsyI GACNNNGTC 1 cut(s) 162
PvuI CGATCG 1 cut(s) 82
RsaI GTAC 2 cut(s) 125, 740
RsaNI GTAC 2 cut(s) 124, 739
SacII CCGCGG 1 cut(s) 205
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 4 cut(s) 39, 300, 798, 1138
SatI GCNGC 3 cut(s) 633, 682, 1065
Sau3AI GATC 7 cut(s) 79, 151, 589, 610, 721, 814, 1030
Sau96I GGNCC 2 cut(s) 466, 654
ScaI AGTACT 1 cut(s) 740
SchI GAGTC 2 cut(s) 839, 970
ScrFI CCNGG 3 cut(s) 54, 470, 658
SduI GDGCHC 1 cut(s) 528
SfaNI GCATC 2 cut(s) 207, 924
Sfr303I CCGCGG 1 cut(s) 205
SgrBI CCGCGG 1 cut(s) 205
SmlI CTYRAG 3 cut(s) 446, 662, 797
SmoI CTYRAG 3 cut(s) 446, 662, 797
SphI GCATGC 1 cut(s) 681
Sse9I AATT 2 cut(s) 18, 946
SsiI CCGC 2 cut(s) 202, 204
StyD4I CCNGG 3 cut(s) 52, 468, 656
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 1 cut(s) 388
TaiI ACGT 2 cut(s) 166, 439
TaqI TCGA 1 cut(s) 29
TasI AATT 2 cut(s) 18, 946
TatI WGTACW 2 cut(s) 123, 738
TfiI GAWTC 7 cut(s) 26, 320, 535, 553, 571, 578, 754
Tru1I TTAA 4 cut(s) 39, 300, 798, 1138
Tru9I TTAA 4 cut(s) 39, 300, 798, 1138
TscAI CASTG 2 cut(s) 393, 607
TseI GCWGC 3 cut(s) 632, 681, 1064
TspDTI ATGAA 7 cut(s) 305, 333, 584, 731, 793, 870, 1085
TspGWI ACGGA 1 cut(s) 827
TspRI CASTG 2 cut(s) 393, 607
Tth111I GACNNNGTC 1 cut(s) 162
Vha464I CTTAAG 1 cut(s) 797
XapI RAATTY 1 cut(s) 946
XceI RCATGY 1 cut(s) 681
XmnI GAANNNNTTC 1 cut(s) 552
ZrmI AGTACT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.