Rw3G020310

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0017431
Physical Location & Seq
Reverse (-)
23755366 .. 23756553
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G020310.1

Sequence Viewer

Length: 1188 bp
ATGGCGATGCGAGGCAAAATTAGGGAATTGACAAAAGTTAAAAGATACTGGCCTGGAAAAGCCCCAGAGTGGGCAAACGATCGTGATACCTGCTCCTCCGACGAAGAAGAGATTACCATCTTGTACAAATCCTTCCCAACTCGTGATGATGATCCTAGACAACGTCGTTTGGCCGAGAGGAGGAGTGTAAAGAGGCGCACCCGCCGTGCCGAGATTGTTGACACAACTGAAGAGGAGGAGGAGGAGGAGGAAGAAGAGGACGCAGAGACTTTGGAGGAAAGAAGAAGAAGGATTAAGGAGAGGTTGCTTCGTCAGATGGAGCAGGAACAACAACAAGAGGAGTTTAAGACTGATGACTACTCCACGGAAGACGAATCCGATGAGGACTACTCAGAAGAAGAAGAGGATGAGATTGCTGGTGAAGTAGTTACGGTGAAGCCTGTATTTTTGTCCAAGTCCACAAGAGACACGATAGTTGAACGTAAGAGACTTGAGGAGGCCGAAGAGCAGGCCCTCGAAGCGAGGAGAAGGGAGCTGGAGGAGAGGAAGAAGGAGACAAGGAGAATGGTGCTCGAAGAGATTCGGAAAGACGAAGAGATTCATAAGGGTTTGATGAATCAGGATTCGGCAAATGATGATGTTGACACTGACATTAATGATCCTGAGGAGGATACTAAAGCAGCTTGGAAGGCAAGAGAGAGGGCCAGGCTCAAGAAGGAGAGGGCAGCAAGGGAAGTAGTCATTTTGAAGGAGATAGAGAAGATGAATGATCAAGTGAGGAGGGAGTACTGGGAGAGGAAGAATCCTCCCTCATCTCCTGCGCCACAGCCCCGAAAGAGGAAGCTTAAGTTCATGCAGAGAGATTACCACAGGGGAGCTTTCTTCCGTTCGGACTCTGATGATGAAGATGGCGTTTTAGGAAGAGATTATTCTACCCCAACCGGAGAGGATAAGATGGATAAAACGAGACTGCCCAAGGTGATGCAGGTCAAGAATTTCGGGCGTAGGGGAAGGGCTAAATGGACTCATCTTGTCAATGAGGATACTACTGATTGGAACAGCCCGTGGATGTGGAATGATCGTCAAAGGTCAGGGTATAATGTGAAAATGGCAGCCATGAATGCACCAATAGCAAGACCAAAAGGAAACAAGAGATTGAGGTTCTCTGTGAAGGAAGATGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

395

Amino Acids

47.37

Weight (kDa)

5.35

Isoelectric Point (pI)

73.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 134 - 349 4.2e-51 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017431)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479581
rosa_laevigata RLG00000023558 RLG00000029558 RLG00000029584
rosa_multiflora Rmu_sc0002232.1_g000002 Rmu_sc0007129.1_g000020
rosa_roxburghii Rroxscaffold_6G00402140
rosa_rugosa Rorug03G0176600
rosa_samantha Rh3BG260500 Rh3CG257300 Rh3CG257900
rosa_wichuraiana Rw3G020310 Rw3G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 98, 976
AciI CCGC 1 cut(s) 202
AclWI GGATC 2 cut(s) 146, 653
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 994
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 2 cut(s) 125, 788
AfiI CCNNNNNNNGG 4 cut(s) 69, 70, 180, 837
AflII CTTAAG 1 cut(s) 845
AgsI TTSAA 2 cut(s) 479, 748
AjnI CCWGG 2 cut(s) 52, 704
AluBI AGCT 4 cut(s) 535, 683, 844, 878
AluI AGCT 4 cut(s) 535, 683, 844, 878
Alw21I GWGCWC 1 cut(s) 573
Alw26I GTCTC 5 cut(s) 260, 459, 481, 548, 961
AlwI GGATC 2 cut(s) 146, 653
AoxI GGCC 5 cut(s) 50, 171, 498, 510, 702
ApeKI GCWGC 3 cut(s) 680, 725, 1112
ApoI RAATTY 1 cut(s) 994
AseI ATTAAT 1 cut(s) 654
Asp700I GAANNNNTTC 2 cut(s) 579, 597
AspLEI GCGC 2 cut(s) 198, 823
AspS9I GGNCC 2 cut(s) 511, 702
AsuHPI GGTGA 3 cut(s) 431, 445, 991
AxyI CCTNAGG 1 cut(s) 663
BarI GAAGNNNNNNTAC 2 cut(s) 116, 148
BauI CACGAG 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 573
BbvI GCAGC 3 cut(s) 692, 737, 1124
BccI CCATC 4 cut(s) 125, 310, 902, 949
BceAI ACGGC 1 cut(s) 189
BciT130I CCWGG 2 cut(s) 54, 706
BciVI GTATCC 2 cut(s) 664, 1036
BclI TGATCA 1 cut(s) 769
BcoDI GTCTC 5 cut(s) 260, 459, 481, 548, 961
BfaI CTAG 1 cut(s) 156
BfrI CTTAAG 1 cut(s) 845
BfuAI ACCTGC 2 cut(s) 98, 976
BfuI GTATCC 2 cut(s) 664, 1036
BisI GCNGC 3 cut(s) 681, 726, 1113
BlsI GCNGC 3 cut(s) 682, 727, 1114
BmcAI AGTACT 1 cut(s) 788
Bme1390I CCNGG 2 cut(s) 54, 706
BmgT120I GGNCC 2 cut(s) 511, 702
BmrFI CCNGG 2 cut(s) 54, 706
BmrI ACTGGG 1 cut(s) 799
BmsI GCATC 1 cut(s) 972
BmuI ACTGGG 1 cut(s) 799
BpiI GAAGAC 1 cut(s) 375
BplI GAGNNNNNCTC 2 cut(s) 374, 406
BpmI CTGGAG 1 cut(s) 557
BpuEI CTTGAG 2 cut(s) 512, 695
BsaBI GATNNNNATC 2 cut(s) 116, 150
BsaJI CCNNGG 3 cut(s) 363, 975, 1064
BsaWI WCCGGW 1 cut(s) 941
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 70, 180, 837
Bse1I ACTGG 2 cut(s) 53, 794
Bse21I CCTNAGG 1 cut(s) 663
Bse8I GATNNNNATC 2 cut(s) 116, 150
BseBI CCWGG 2 cut(s) 54, 706
BseDI CCNNGG 3 cut(s) 363, 975, 1064
BseGI GGATG 2 cut(s) 412, 1074
BseJI GATNNNNATC 2 cut(s) 116, 150
BseLI CCNNNNNNNGG 4 cut(s) 69, 70, 180, 837
BseMII CTCAG 2 cut(s) 405, 654
BseNI ACTGG 2 cut(s) 53, 794
BseXI GCAGC 3 cut(s) 692, 737, 1124
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 5 cut(s) 52, 173, 500, 512, 704
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 573
BsiSI CCGG 1 cut(s) 942
BslI CCNNNNNNNGG 4 cut(s) 69, 70, 180, 837
BsmAI GTCTC 5 cut(s) 260, 459, 481, 548, 961
BsmI GAATGC 1 cut(s) 1126
BsnI GGCC 5 cut(s) 52, 173, 500, 512, 704
Bsp1286I GDGCHC 1 cut(s) 573
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 5 cut(s) 79, 151, 658, 769, 1078
BspACI CCGC 1 cut(s) 202
BspANI GGCC 5 cut(s) 52, 173, 500, 512, 704
BspCNI CTCAG 2 cut(s) 404, 655
BspMI ACCTGC 2 cut(s) 98, 976
BspPI GGATC 2 cut(s) 146, 653
BspQI GCTCTTC 1 cut(s) 498
BspTI CTTAAG 1 cut(s) 845
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 2 cut(s) 53, 794
BssECI CCNNGG 3 cut(s) 363, 975, 1064
BssMI GATC 5 cut(s) 79, 151, 658, 769, 1078
BssSI CACGAG 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 975
Bst2BI CACGAG 1 cut(s) 141
Bst2UI CCWGG 2 cut(s) 54, 706
Bst4CI ACNGT 1 cut(s) 433
Bst6I CTCTTC 8 cut(s) 102, 225, 249, 396, 498, 570, 588, 916
BstAFI CTTAAG 1 cut(s) 845
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 2 cut(s) 391, 663
BstDSI CCRYGG 2 cut(s) 363, 1064
BstF5I GGATG 2 cut(s) 412, 1074
BstHHI GCGC 2 cut(s) 198, 823
BstKTI GATC 5 cut(s) 82, 154, 661, 772, 1081
BstMAI GTCTC 5 cut(s) 260, 459, 481, 548, 961
BstMBI GATC 5 cut(s) 79, 151, 658, 769, 1078
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 4 cut(s) 518, 689, 1121, 1130
BstNI CCWGG 2 cut(s) 54, 706
BstSCI CCNGG 2 cut(s) 52, 704
BstV1I GCAGC 3 cut(s) 692, 737, 1124
BstV2I GAAGAC 1 cut(s) 375
Bsu36I CCTNAGG 1 cut(s) 663
BsuI GTATCC 2 cut(s) 664, 1036
BsuRI GGCC 5 cut(s) 52, 173, 500, 512, 704
BtgI CCRYGG 2 cut(s) 363, 1064
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 412, 1074
BtsIMutI CAGTG 1 cut(s) 645
BveI ACCTGC 2 cut(s) 98, 976
Cac8I GCNNGC 1 cut(s) 510
CfoI GCGC 2 cut(s) 198, 823
Cfr13I GGNCC 2 cut(s) 511, 702
CseI GACGC 1 cut(s) 269
Csp6I GTAC 2 cut(s) 124, 787
CviAII CATG 2 cut(s) 853, 1117
CviQI GTAC 2 cut(s) 124, 787
DdeI CTNAG 2 cut(s) 391, 663
DpnI GATC 5 cut(s) 81, 153, 660, 771, 1080
DpnII GATC 5 cut(s) 79, 151, 658, 769, 1078
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 8 cut(s) 102, 225, 249, 396, 498, 570, 588, 916
EarI CTCTTC 8 cut(s) 102, 225, 249, 396, 498, 570, 588, 916
Eco130I CCWWGG 1 cut(s) 975
Eco57I CTGAAG 1 cut(s) 249
Eco81I CCTNAGG 1 cut(s) 663
EcoO109I RGGNCCY 1 cut(s) 511
EcoRII CCWGG 2 cut(s) 52, 704
EcoT14I CCWWGG 1 cut(s) 975
ErhI CCWWGG 1 cut(s) 975
FaeI CATG 2 cut(s) 856, 1120
FaiI YATR 4 cut(s) 603, 854, 1098, 1118
FalI AAGNNNNNCTT 1 cut(s) 1167
FatI CATG 2 cut(s) 852, 1116
FauI CCCGC 1 cut(s) 209
FbaI TGATCA 1 cut(s) 769
Fnu4HI GCNGC 3 cut(s) 681, 726, 1113
FokI GGATG 2 cut(s) 419, 1081
Fsp4HI GCNGC 3 cut(s) 681, 726, 1113
FspBI CTAG 1 cut(s) 156
GlaI GCGC 2 cut(s) 197, 822
GluI GCNGC 3 cut(s) 681, 726, 1113
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 5 cut(s) 52, 173, 500, 512, 704
HapII CCGG 1 cut(s) 942
HgaI GACGC 1 cut(s) 269
HhaI GCGC 2 cut(s) 198, 823
Hin1II CATG 2 cut(s) 856, 1120
Hin6I GCGC 2 cut(s) 196, 821
HinP1I GCGC 2 cut(s) 196, 821
HincII GTYRAC 2 cut(s) 220, 643
HindII GTYRAC 2 cut(s) 220, 643
HindIII AAGCTT 1 cut(s) 842
HinfI GANTC 8 cut(s) 374, 580, 598, 616, 623, 802, 893, 1024
HpaII CCGG 1 cut(s) 942
HphI GGTGA 3 cut(s) 431, 445, 991
Hpy166II GTNNAC 3 cut(s) 220, 459, 643
Hpy188I TCNGA 7 cut(s) 100, 315, 379, 394, 585, 892, 898
Hpy188III TCNNGA 6 cut(s) 83, 143, 620, 662, 712, 991
Hpy8I GTNNAC 3 cut(s) 220, 459, 643
Hpy99I CGWCG 2 cut(s) 104, 168
HpyAV CCTTC 9 cut(s) 142, 282, 522, 544, 682, 709, 742, 1005, 1165
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 2 cut(s) 163, 481
HpyCH4V TGCA 3 cut(s) 856, 985, 1124
HpyF10VI GCNNNNNNNGC 4 cut(s) 518, 689, 1121, 1130
HpyF3I CTNAG 2 cut(s) 391, 663
HpySE526I ACGT 2 cut(s) 163, 481
Hsp92II CATG 2 cut(s) 856, 1120
HspAI GCGC 2 cut(s) 196, 821
Ksp22I TGATCA 1 cut(s) 769
Kzo9I GATC 5 cut(s) 79, 151, 658, 769, 1078
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 4 cut(s) 98, 319, 532, 875
Lsp1109I GCAGC 3 cut(s) 692, 737, 1124
LweI GCATC 1 cut(s) 972
MaeI CTAG 1 cut(s) 156
MaeII ACGT 2 cut(s) 163, 481
MaeIII GTNAC 1 cut(s) 427
MalI GATC 5 cut(s) 81, 153, 660, 771, 1080
MboI GATC 5 cut(s) 79, 151, 658, 769, 1078
MhlI GDGCHC 1 cut(s) 573
MluCI AATT 3 cut(s) 18, 26, 994
MlyI GAGTC 2 cut(s) 887, 1018
MmeI TCCRAC 1 cut(s) 123
MroXI GAANNNNTTC 2 cut(s) 579, 597
MseI TTAA 6 cut(s) 39, 294, 345, 654, 846, 1186
MspCI CTTAAG 1 cut(s) 845
MspI CCGG 1 cut(s) 942
MspR9I CCNGG 2 cut(s) 54, 706
Mva1269I GAATGC 1 cut(s) 1126
MvaI CCWGG 2 cut(s) 54, 706
MwoI GCNNNNNNNGC 4 cut(s) 518, 689, 1121, 1130
NdeII GATC 5 cut(s) 79, 151, 658, 769, 1078
NlaIII CATG 2 cut(s) 856, 1120
NmeAIII GCCGAG 2 cut(s) 199, 235
PciSI GCTCTTC 1 cut(s) 498
PctI GAATGC 1 cut(s) 1126
PdmI GAANNNNTTC 2 cut(s) 579, 597
PfeI GAWTC 6 cut(s) 374, 580, 598, 616, 623, 802
PflFI GACNNNGTC 1 cut(s) 162
PkrI GCNGC 3 cut(s) 682, 727, 1114
Ple19I CGATCG 1 cut(s) 82
PleI GAGTC 2 cut(s) 887, 1018
PpsI GAGTC 2 cut(s) 887, 1018
PshBI ATTAAT 1 cut(s) 654
Psp6I CCWGG 2 cut(s) 52, 704
PspGI CCWGG 2 cut(s) 52, 704
PspPI GGNCC 2 cut(s) 511, 702
PsyI GACNNNGTC 1 cut(s) 162
PvuI CGATCG 1 cut(s) 82
RsaI GTAC 2 cut(s) 125, 788
RsaNI GTAC 2 cut(s) 124, 787
SapI GCTCTTC 1 cut(s) 498
SaqAI TTAA 6 cut(s) 39, 294, 345, 654, 846, 1186
SatI GCNGC 3 cut(s) 681, 726, 1113
Sau3AI GATC 5 cut(s) 79, 151, 658, 769, 1078
Sau96I GGNCC 2 cut(s) 511, 702
ScaI AGTACT 1 cut(s) 788
SchI GAGTC 2 cut(s) 887, 1018
ScrFI CCNGG 2 cut(s) 54, 706
SduI GDGCHC 1 cut(s) 573
SfaNI GCATC 1 cut(s) 972
SmlI CTYRAG 3 cut(s) 491, 710, 845
SmoI CTYRAG 3 cut(s) 491, 710, 845
Sse9I AATT 3 cut(s) 18, 26, 994
SsiI CCGC 1 cut(s) 202
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 52, 704
StyI CCWWGG 1 cut(s) 975
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 2 cut(s) 166, 484
TaqI TCGA 2 cut(s) 516, 573
TasI AATT 3 cut(s) 18, 26, 994
TatI WGTACW 2 cut(s) 123, 786
TfiI GAWTC 6 cut(s) 374, 580, 598, 616, 623, 802
Tru1I TTAA 6 cut(s) 39, 294, 345, 654, 846, 1186
Tru9I TTAA 6 cut(s) 39, 294, 345, 654, 846, 1186
TscAI CASTG 1 cut(s) 652
TseI GCWGC 3 cut(s) 680, 725, 1112
TspDTI ATGAA 6 cut(s) 590, 629, 779, 841, 918, 1133
TspGWI ACGGA 2 cut(s) 380, 875
TspRI CASTG 1 cut(s) 652
Tth111I GACNNNGTC 1 cut(s) 162
Vha464I CTTAAG 1 cut(s) 845
VspI ATTAAT 1 cut(s) 654
XapI RAATTY 1 cut(s) 994
XmnI GAANNNNTTC 2 cut(s) 579, 597
XspI CTAG 1 cut(s) 156
ZrmI AGTACT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.