Rroxscaffold_6G00402140

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017431
Physical Location & Seq
Forward (+)
24516387 .. 24524715
8329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00402140.1

Sequence Viewer

Length: 1245 bp
ATGGCGATGCGAGGCAAAATTAGGGAATCGACAAAAGTTAAAAGATACTGGCCTGGAAAAGCGCCAGAGTGGGCAAACGATCGTGATACCTGCTCCTCCGACGAAGAAGGGATTACCATCTTGTACAAATCCTTCTCAACTCGTGATGATGATCCTAGACTACGTCGTTTGGCCGAGAGGAGGAGTGTAAAGAGGCGCATCCGCCTTGCCGAGATTGTTGACACAACTGAAGAGGAGGAGGAGGAGGAAGAAGAAGAGGACGCAGAGACTTTGGAGGAAAGAAGAAGAAGGATTAAGGAGAGGTTGCTTCGTCAGAAGGAGCAGGAACAACAACAAGAGGAGTTTAAGACTGATGACTACTCCACGGAAGACGAATCCGATGAGGACTACTCAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGGATGAGATTGCTGGTGAAGTAGTTACGGTGAAGCCTGTATTTTTGTCCAAGTCCACAAGAGACACGATAGTTGAACGTAAGAGACTTGAGGAGGCTGAAGAGCAGGCCCTGGAAGCGAGGAGAAGGGAGCTGGAGGAGAGGAAGAAAGAGACAAGGACAATGGTGCTCGAAGAGATTCGGAAAGACGAAGAGATTCACAAGGGTTTGATGAATCAGGATTCGGCAAATGATGATGTTGACACTGACAATGATCCTGAGGAGGATACTAAAGCAGCTTGGAAGGCAAGAGAGAGAGCCAGGCTCAGGAAGGAGAGGGCAGCAAGGGAAGCAGTCATTTTGAAGGAGATGGAGAAGATGAATGATCAAGAGAGGAGGGAGTACTACTGGGAGAGGAAGAATCCTCCCTCACCTCCTGCGCCACAGCCCCGAAAGAGGAAGCTTAAGTTCATGCAGAGAGATCACCACAGGGGAGCTTTCTTCCGTTCGGACTCTGATGATGAAGATGGCGTTTTAGGAAGAGATTATTCTGCCCCAACCGGAGAGGATAGGATGGATAAAACAAGACTGCCCAAGGTGATGCAGGTCAAGAATTTCGGGCGTAGGGGAAGGGCTAAATGGACTCATCTTGTCAATGAGGATACTACTGATTGGAACAGCCCGTGGATGTGGAATGATCGTCAAAGGTCAAGGTATAATGTGAAAATGGCAGCCATGAATGCACCAATAGCAAGACCAAAAGGTAACAAGAGATTGAGGTTCTCTGTGAAGGAAGATGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

414

Amino Acids

49.79

Weight (kDa)

4.89

Isoelectric Point (pI)

81.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 153 - 368 6.8e-50 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017431)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479581
rosa_laevigata RLG00000023558 RLG00000029558 RLG00000029584
rosa_multiflora Rmu_sc0002232.1_g000002 Rmu_sc0007129.1_g000020
rosa_roxburghii Rroxscaffold_6G00402140
rosa_rugosa Rorug03G0176600
rosa_samantha Rh3BG260500 Rh3CG257300 Rh3CG257900
rosa_wichuraiana Rw3G020310 Rw3G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 98, 1033
AciI CCGC 1 cut(s) 202
AclWI GGATC 2 cut(s) 146, 707
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 1051
AcuI CTGAAG 2 cut(s) 249, 579
AfaI GTAC 2 cut(s) 125, 842
AfiI CCNNNNNNNGG 3 cut(s) 180, 765, 894
AflII CTTAAG 1 cut(s) 902
AgsI TTSAA 2 cut(s) 536, 802
AjnI CCWGG 3 cut(s) 52, 570, 758
AluBI AGCT 4 cut(s) 592, 737, 901, 935
AluI AGCT 4 cut(s) 592, 737, 901, 935
Alw21I GWGCWC 1 cut(s) 630
Alw26I GTCTC 4 cut(s) 260, 516, 538, 605
AlwI GGATC 2 cut(s) 146, 707
AlwNI CAGNNNCTG 1 cut(s) 571
AoxI GGCC 3 cut(s) 50, 171, 567
ApeKI GCWGC 3 cut(s) 734, 779, 1169
ApoI RAATTY 1 cut(s) 1051
Asp700I GAANNNNTTC 2 cut(s) 636, 654
AspLEI GCGC 3 cut(s) 64, 198, 880
AspS9I GGNCC 1 cut(s) 568
AsuHPI GGTGA 5 cut(s) 488, 502, 861, 914, 1048
AxyI CCTNAGG 1 cut(s) 717
BarI GAAGNNNNNNTAC 2 cut(s) 116, 148
BauI CACGAG 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 630
BbvI GCAGC 3 cut(s) 746, 791, 1181
BccI CCATC 4 cut(s) 125, 802, 959, 1006
BciT130I CCWGG 3 cut(s) 54, 572, 760
BciVI GTATCC 2 cut(s) 718, 1093
BclI TGATCA 1 cut(s) 823
BcoDI GTCTC 4 cut(s) 260, 516, 538, 605
BfaI CTAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 65
BfrI CTTAAG 1 cut(s) 902
BfuAI ACCTGC 2 cut(s) 98, 1033
BfuI GTATCC 2 cut(s) 718, 1093
BisI GCNGC 3 cut(s) 735, 780, 1170
BlsI GCNGC 3 cut(s) 736, 781, 1171
BmcAI AGTACT 1 cut(s) 842
Bme1390I CCNGG 3 cut(s) 54, 572, 760
BmgT120I GGNCC 1 cut(s) 568
BmrFI CCNGG 3 cut(s) 54, 572, 760
BmrI ACTGGG 1 cut(s) 856
BmsI GCATC 2 cut(s) 207, 1029
BmuI ACTGGG 1 cut(s) 856
BpiI GAAGAC 1 cut(s) 375
BplI GAGNNNNNCTC 4 cut(s) 374, 406, 747, 779
BpmI CTGGAG 1 cut(s) 614
Bpu10I CCTNAGC 1 cut(s) 764
BpuEI CTTGAG 1 cut(s) 569
BsaBI GATNNNNATC 2 cut(s) 116, 150
BsaJI CCNNGG 4 cut(s) 363, 570, 1032, 1121
BsaWI WCCGGW 1 cut(s) 998
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 765, 894
Bse1I ACTGG 2 cut(s) 53, 851
Bse21I CCTNAGG 1 cut(s) 717
Bse8I GATNNNNATC 2 cut(s) 116, 150
BseBI CCWGG 3 cut(s) 54, 572, 760
BseDI CCNNGG 4 cut(s) 363, 570, 1032, 1121
BseGI GGATG 4 cut(s) 198, 469, 1017, 1131
BseJI GATNNNNATC 2 cut(s) 116, 150
BseLI CCNNNNNNNGG 3 cut(s) 180, 765, 894
BseMII CTCAG 3 cut(s) 405, 708, 778
BseNI ACTGG 2 cut(s) 53, 851
BseXI GCAGC 3 cut(s) 746, 791, 1181
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 3 cut(s) 52, 173, 569
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 630
BsiSI CCGG 1 cut(s) 999
BslI CCNNNNNNNGG 3 cut(s) 180, 765, 894
BsmAI GTCTC 4 cut(s) 260, 516, 538, 605
BsmI GAATGC 1 cut(s) 1183
BsnI GGCC 3 cut(s) 52, 173, 569
Bsp1286I GDGCHC 1 cut(s) 630
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 6 cut(s) 79, 151, 712, 823, 919, 1135
BspACI CCGC 1 cut(s) 202
BspANI GGCC 3 cut(s) 52, 173, 569
BspCNI CTCAG 3 cut(s) 404, 709, 777
BspMI ACCTGC 2 cut(s) 98, 1033
BspPI GGATC 2 cut(s) 146, 707
BspQI GCTCTTC 1 cut(s) 555
BspTI CTTAAG 1 cut(s) 902
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 2 cut(s) 53, 851
BssECI CCNNGG 4 cut(s) 363, 570, 1032, 1121
BssMI GATC 6 cut(s) 79, 151, 712, 823, 919, 1135
BssSI CACGAG 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 1032
Bst2BI CACGAG 1 cut(s) 141
Bst2UI CCWGG 3 cut(s) 54, 572, 760
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 7 cut(s) 225, 249, 453, 555, 627, 645, 973
BstAFI CTTAAG 1 cut(s) 902
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 3 cut(s) 391, 717, 764
BstDSI CCRYGG 2 cut(s) 363, 1121
BstF5I GGATG 4 cut(s) 198, 469, 1017, 1131
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 3 cut(s) 64, 198, 880
BstKTI GATC 6 cut(s) 82, 154, 715, 826, 922, 1138
BstMAI GTCTC 4 cut(s) 260, 516, 538, 605
BstMBI GATC 6 cut(s) 79, 151, 712, 823, 919, 1135
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 5 cut(s) 575, 743, 788, 1178, 1187
BstNI CCWGG 3 cut(s) 54, 572, 760
BstSCI CCNGG 3 cut(s) 52, 570, 758
BstV1I GCAGC 3 cut(s) 746, 791, 1181
BstV2I GAAGAC 1 cut(s) 375
Bsu36I CCTNAGG 1 cut(s) 717
BsuI GTATCC 2 cut(s) 718, 1093
BsuRI GGCC 3 cut(s) 52, 173, 569
BtgI CCRYGG 2 cut(s) 363, 1121
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 4 cut(s) 198, 469, 1017, 1131
BtsIMutI CAGTG 1 cut(s) 702
BveI ACCTGC 2 cut(s) 98, 1033
Cac8I GCNNGC 1 cut(s) 567
CaiI CAGNNNCTG 1 cut(s) 571
CfoI GCGC 3 cut(s) 64, 198, 880
Cfr13I GGNCC 1 cut(s) 568
CseI GACGC 1 cut(s) 269
Csp6I GTAC 2 cut(s) 124, 841
CviAII CATG 2 cut(s) 910, 1174
CviQI GTAC 2 cut(s) 124, 841
DdeI CTNAG 3 cut(s) 391, 717, 764
DpnI GATC 6 cut(s) 81, 153, 714, 825, 921, 1137
DpnII GATC 6 cut(s) 79, 151, 712, 823, 919, 1135
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 7 cut(s) 225, 249, 453, 555, 627, 645, 973
EarI CTCTTC 7 cut(s) 225, 249, 453, 555, 627, 645, 973
EciI GGCGGA 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 1032
Eco57I CTGAAG 2 cut(s) 249, 579
Eco81I CCTNAGG 1 cut(s) 717
EcoO109I RGGNCCY 1 cut(s) 568
EcoRII CCWGG 3 cut(s) 52, 570, 758
EcoT14I CCWWGG 1 cut(s) 1032
ErhI CCWWGG 1 cut(s) 1032
FaeI CATG 2 cut(s) 913, 1177
FaiI YATR 3 cut(s) 911, 1155, 1175
FalI AAGNNNNNCTT 1 cut(s) 1224
FatI CATG 2 cut(s) 909, 1173
FbaI TGATCA 1 cut(s) 823
Fnu4HI GCNGC 3 cut(s) 735, 780, 1170
FokI GGATG 4 cut(s) 185, 476, 1024, 1138
Fsp4HI GCNGC 3 cut(s) 735, 780, 1170
FspBI CTAG 1 cut(s) 156
GlaI GCGC 3 cut(s) 63, 197, 879
GluI GCNGC 3 cut(s) 735, 780, 1170
GsuI CTGGAG 1 cut(s) 614
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 3 cut(s) 52, 173, 569
HapII CCGG 1 cut(s) 999
HgaI GACGC 1 cut(s) 269
HhaI GCGC 3 cut(s) 64, 198, 880
Hin1II CATG 2 cut(s) 913, 1177
Hin6I GCGC 3 cut(s) 62, 196, 878
HinP1I GCGC 3 cut(s) 62, 196, 878
HincII GTYRAC 2 cut(s) 220, 700
HindII GTYRAC 2 cut(s) 220, 700
HindIII AAGCTT 1 cut(s) 899
HinfI GANTC 9 cut(s) 26, 374, 637, 655, 673, 680, 859, 950, 1081
HpaII CCGG 1 cut(s) 999
HphI GGTGA 5 cut(s) 488, 502, 861, 914, 1048
Hpy166II GTNNAC 3 cut(s) 220, 516, 700
Hpy188I TCNGA 7 cut(s) 100, 315, 379, 394, 642, 949, 955
Hpy188III TCNNGA 7 cut(s) 83, 143, 677, 716, 766, 827, 1048
Hpy8I GTNNAC 3 cut(s) 220, 516, 700
Hpy99I CGWCG 2 cut(s) 104, 168
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4IV ACGT 2 cut(s) 163, 538
HpyCH4V TGCA 3 cut(s) 913, 1042, 1181
HpyF10VI GCNNNNNNNGC 5 cut(s) 575, 743, 788, 1178, 1187
HpyF3I CTNAG 3 cut(s) 391, 717, 764
HpySE526I ACGT 2 cut(s) 163, 538
Hsp92II CATG 2 cut(s) 913, 1177
HspAI GCGC 3 cut(s) 62, 196, 878
Ksp22I TGATCA 1 cut(s) 823
Kzo9I GATC 6 cut(s) 79, 151, 712, 823, 919, 1135
LguI GCTCTTC 1 cut(s) 555
LmnI GCTCC 4 cut(s) 98, 319, 589, 932
Lsp1109I GCAGC 3 cut(s) 746, 791, 1181
LweI GCATC 2 cut(s) 207, 1029
MaeI CTAG 1 cut(s) 156
MaeII ACGT 2 cut(s) 163, 538
MaeIII GTNAC 2 cut(s) 484, 1202
MalI GATC 6 cut(s) 81, 153, 714, 825, 921, 1137
MboI GATC 6 cut(s) 79, 151, 712, 823, 919, 1135
MhlI GDGCHC 1 cut(s) 630
MluCI AATT 2 cut(s) 18, 1051
MlyI GAGTC 2 cut(s) 944, 1075
MmeI TCCRAC 1 cut(s) 123
MroXI GAANNNNTTC 2 cut(s) 636, 654
MseI TTAA 5 cut(s) 39, 294, 345, 903, 1243
MspCI CTTAAG 1 cut(s) 902
MspI CCGG 1 cut(s) 999
MspR9I CCNGG 3 cut(s) 54, 572, 760
Mva1269I GAATGC 1 cut(s) 1183
MvaI CCWGG 3 cut(s) 54, 572, 760
MwoI GCNNNNNNNGC 5 cut(s) 575, 743, 788, 1178, 1187
NdeII GATC 6 cut(s) 79, 151, 712, 823, 919, 1135
NlaIII CATG 2 cut(s) 913, 1177
NmeAIII GCCGAG 2 cut(s) 199, 235
PciSI GCTCTTC 1 cut(s) 555
PctI GAATGC 1 cut(s) 1183
PdmI GAANNNNTTC 2 cut(s) 636, 654
PfeI GAWTC 7 cut(s) 26, 374, 637, 655, 673, 680, 859
PflFI GACNNNGTC 1 cut(s) 162
PkrI GCNGC 3 cut(s) 736, 781, 1171
Ple19I CGATCG 1 cut(s) 82
PleI GAGTC 2 cut(s) 944, 1075
PpsI GAGTC 2 cut(s) 944, 1075
Psp6I CCWGG 3 cut(s) 52, 570, 758
PspGI CCWGG 3 cut(s) 52, 570, 758
PspPI GGNCC 1 cut(s) 568
PstNI CAGNNNCTG 1 cut(s) 571
PsyI GACNNNGTC 1 cut(s) 162
PvuI CGATCG 1 cut(s) 82
RsaI GTAC 2 cut(s) 125, 842
RsaNI GTAC 2 cut(s) 124, 841
SapI GCTCTTC 1 cut(s) 555
SaqAI TTAA 5 cut(s) 39, 294, 345, 903, 1243
SatI GCNGC 3 cut(s) 735, 780, 1170
Sau3AI GATC 6 cut(s) 79, 151, 712, 823, 919, 1135
Sau96I GGNCC 1 cut(s) 568
ScaI AGTACT 1 cut(s) 842
SchI GAGTC 2 cut(s) 944, 1075
ScrFI CCNGG 3 cut(s) 54, 572, 760
SduI GDGCHC 1 cut(s) 630
SfaNI GCATC 2 cut(s) 207, 1029
SmlI CTYRAG 2 cut(s) 548, 902
SmoI CTYRAG 2 cut(s) 548, 902
Sse9I AATT 2 cut(s) 18, 1051
SsiI CCGC 1 cut(s) 202
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 3 cut(s) 52, 570, 758
StyI CCWWGG 1 cut(s) 1032
TaaI ACNGT 1 cut(s) 490
TaiI ACGT 2 cut(s) 166, 541
TaqI TCGA 2 cut(s) 29, 630
TasI AATT 2 cut(s) 18, 1051
TatI WGTACW 2 cut(s) 123, 840
TfiI GAWTC 7 cut(s) 26, 374, 637, 655, 673, 680, 859
Tru1I TTAA 5 cut(s) 39, 294, 345, 903, 1243
Tru9I TTAA 5 cut(s) 39, 294, 345, 903, 1243
TscAI CASTG 1 cut(s) 709
TseI GCWGC 3 cut(s) 734, 779, 1169
TspDTI ATGAA 5 cut(s) 686, 833, 898, 975, 1190
TspGWI ACGGA 2 cut(s) 380, 932
TspRI CASTG 1 cut(s) 709
Tth111I GACNNNGTC 1 cut(s) 162
Vha464I CTTAAG 1 cut(s) 902
XapI RAATTY 1 cut(s) 1051
XmnI GAANNNNTTC 2 cut(s) 636, 654
XspI CTAG 1 cut(s) 156
ZrmI AGTACT 1 cut(s) 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.