RLG00000029584

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017431
Physical Location & Seq
Forward (+)
40744549 .. 40746865
2317 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029584

Sequence Viewer

Length: 966 bp
ATGAAGATCATAAGAGGCGCATCAGACTTGTCGAGACTGTTGACAGAGCTGAAGAGGAGGAGGAAGAAGAAGAGGACGCAGTGGATTTGGAGGAAAGAAGAAGGATTAACGAGAGGTTGCTCCATCAGAGGGAGTAAGAACAACAAGACTAAGAGCTTGAGACTGATGATTACTACTCGGAAGACAAATCCGATGGGTATTACTCAGAAGAAGATGAAGATGAAGAGACTGTCGGATTCGGTAAATGATGATGTCGACATTGACGATAATCTTGAGGAGGATATTAAAGCTTCGAGGGCAAGAGAGAGGGCGAGGCTCAAAAAGGAGAGGGAAGCAAAAAAAGCAATCATTATGAAGGAGATAGAGATTGAGAGACTGAAGAAGAGAGATGATCACAGGGGAGTTTTCTTCCGTTCAGACTCTGATGATGAAGATGGCGTTTTAGGAAAAGATGATTCTTCCCCAACCCGAGAGGATAAGATGGATAAAACAAGACTGTCTAAGGTGATGCAGGTCAAGAATTTCGGGTATACAGGAATGGCTAAATGGACTCATGTTGTCAATGAGGACACACTAATTGGAACAACCCATGGATGTGGAATAATTGTCAAAGATCAAGGTACGAACACAACAACGACACGATGTCAAAGTCCTCGTAGCAATATGGGCACGAGAATTCAGGTCGGACCTCGTCCACCTCAAAATCATCCATGCTCAACCGACATCGGAGCTCTGGTGATGGTGCTGCAACGTGAACTGAGGTTTCGCGACGGCGAGGCGGGAAGTCCAGAACTCGGAATCCATCTTGAACGATTTCGACTGATTCGATCAAATTCGAAATCAAAATCAAACTCGATCCAAAATTCCCCAATTTCTGATTCCCAATCTTTGAATCCGGACGACTCTCCGGCTTTGAGCTTTTCCAGAAATTGGAAAGCTAGATCTGGATCAGGATGCGGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

322

Amino Acids

36.46

Weight (kDa)

10.08

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 132 - 191 1.3e-14 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017431)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479581
rosa_laevigata RLG00000023558 RLG00000029558 RLG00000029584
rosa_multiflora Rmu_sc0002232.1_g000002 Rmu_sc0007129.1_g000020
rosa_roxburghii Rroxscaffold_6G00402140
rosa_rugosa Rorug03G0176600
rosa_samantha Rh3BG260500 Rh3CG257300 Rh3CG257900
rosa_wichuraiana Rw3G020310 Rw3G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 502
AccB7I CCANNNNNTGG 1 cut(s) 930
AccI GTMKAC 2 cut(s) 255, 530
AccII CGCG 1 cut(s) 768
AccIII TCCGGA 1 cut(s) 895
AciI CCGC 2 cut(s) 779, 957
AclWI GGATC 2 cut(s) 850, 955
AcsI RAATTY 4 cut(s) 520, 675, 832, 862
AcuI CTGAAG 2 cut(s) 71, 398
AfaI GTAC 1 cut(s) 622
AfiI CCNNNNNNNGG 3 cut(s) 129, 794, 930
AgsI TTSAA 2 cut(s) 809, 892
AhdI GACNNNNNGTC 1 cut(s) 642
AjuI GAANNNNNNNTTGG 2 cut(s) 862, 894
AloI GAACNNNNNNTCC 2 cut(s) 783, 815
AluBI AGCT 6 cut(s) 49, 156, 290, 731, 918, 938
AluI AGCT 6 cut(s) 49, 156, 290, 731, 918, 938
Alw21I GWGCWC 1 cut(s) 733
Alw26I GTCTC 4 cut(s) 28, 154, 220, 367
AlwI GGATC 2 cut(s) 850, 955
AlwNI CAGNNNCTG 1 cut(s) 422
Ama87I CYCGRG 1 cut(s) 468
Aor13HI TCCGGA 1 cut(s) 895
ApeKI GCWGC 1 cut(s) 745
ApoI RAATTY 4 cut(s) 520, 675, 832, 862
Asp700I GAANNNNTTC 1 cut(s) 813
AspLEI GCGC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 686
AsuHPI GGTGA 2 cut(s) 517, 748
AsuII TTCGAA 1 cut(s) 836
AvaI CYCGRG 1 cut(s) 468
AvaII GGWCC 1 cut(s) 686
BaeGI GKGCMC 1 cut(s) 671
BanII GRGCYC 1 cut(s) 733
BauI CACGAG 1 cut(s) 670
BbsI GAAGAC 1 cut(s) 188
Bbv12I GWGCWC 1 cut(s) 733
BbvI GCAGC 1 cut(s) 732
BccI CCATC 6 cut(s) 131, 187, 428, 475, 733, 810
BceAI ACGGC 1 cut(s) 787
BclI TGATCA 1 cut(s) 391
BcoDI GTCTC 4 cut(s) 28, 154, 220, 367
BfaI CTAG 1 cut(s) 939
BfuAI ACCTGC 1 cut(s) 502
BglII AGATCT 1 cut(s) 941
BisI GCNGC 1 cut(s) 746
BlsI GCNGC 1 cut(s) 747
Bme18I GGWCC 1 cut(s) 686
BmeRI GACNNNNNGTC 1 cut(s) 642
BmeT110I CYCGRG 1 cut(s) 468
BmgT120I GGNCC 1 cut(s) 686
BmsI GCATC 3 cut(s) 29, 498, 944
BpiI GAAGAC 1 cut(s) 188
Bpu14I TTCGAA 1 cut(s) 836
BpuEI CTTGAG 2 cut(s) 178, 293
BsaBI GATNNNNATC 1 cut(s) 946
BsaJI CCNNGG 1 cut(s) 589
BsaWI WCCGGW 1 cut(s) 895
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 794, 930
Bse8I GATNNNNATC 1 cut(s) 946
BseAI TCCGGA 1 cut(s) 895
BseDI CCNNGG 1 cut(s) 589
BseGI GGATG 3 cut(s) 599, 706, 959
BseJI GATNNNNATC 1 cut(s) 946
BseLI CCNNNNNNNGG 3 cut(s) 129, 794, 930
BseMII CTCAG 2 cut(s) 218, 749
BseRI GAGGAG 3 cut(s) 70, 73, 290
BseSI GKGCMC 1 cut(s) 671
BseXI GCAGC 1 cut(s) 732
Bsh1236I CGCG 1 cut(s) 768
BsiHKAI GWGCWC 1 cut(s) 733
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 2 cut(s) 896, 908
BslI CCNNNNNNNGG 3 cut(s) 129, 794, 930
BsmAI GTCTC 4 cut(s) 28, 154, 220, 367
BsoBI CYCGRG 1 cut(s) 468
Bsp119I TTCGAA 1 cut(s) 836
Bsp1286I GDGCHC 2 cut(s) 671, 733
Bsp13I TCCGGA 1 cut(s) 895
Bsp143I GATC 7 cut(s) 6, 391, 613, 827, 855, 941, 947
Bsp19I CCATGG 1 cut(s) 589
Bsp68I TCGCGA 1 cut(s) 768
BspACI CCGC 2 cut(s) 779, 957
BspCNI CTCAG 2 cut(s) 217, 750
BspEI TCCGGA 1 cut(s) 895
BspFNI CGCG 1 cut(s) 768
BspMI ACCTGC 1 cut(s) 502
BspPI GGATC 2 cut(s) 850, 955
BspT104I TTCGAA 1 cut(s) 836
BssECI CCNNGG 1 cut(s) 589
BssMI GATC 7 cut(s) 6, 391, 613, 827, 855, 941, 947
BssNAI GTATAC 1 cut(s) 531
BssSI CACGAG 1 cut(s) 670
BssT1I CCWWGG 1 cut(s) 589
Bst1107I GTATAC 1 cut(s) 531
Bst2BI CACGAG 1 cut(s) 670
Bst4CI ACNGT 3 cut(s) 39, 231, 498
Bst6I CTCTTC 4 cut(s) 47, 65, 218, 377
BstBI TTCGAA 1 cut(s) 836
BstDEI CTNAG 4 cut(s) 150, 204, 501, 758
BstDSI CCRYGG 1 cut(s) 589
BstF5I GGATG 3 cut(s) 599, 706, 959
BstFNI CGCG 1 cut(s) 768
BstHHI GCGC 1 cut(s) 20
BstKTI GATC 7 cut(s) 9, 394, 616, 830, 858, 944, 950
BstMAI GTCTC 4 cut(s) 28, 154, 220, 367
BstMBI GATC 7 cut(s) 6, 391, 613, 827, 855, 941, 947
BstMWI GCNNNNNNNGC 3 cut(s) 296, 341, 666
BstSLI GKGCMC 1 cut(s) 671
BstUI CGCG 1 cut(s) 768
BstV1I GCAGC 1 cut(s) 732
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 941
BstXI CCANNNNNNTGG 1 cut(s) 596
BstYI RGATCY 1 cut(s) 941
BstZ17I GTATAC 1 cut(s) 531
BtgI CCRYGG 1 cut(s) 589
BtsCI GGATG 3 cut(s) 599, 706, 959
BtsI GCAGTG 1 cut(s) 86
BtsIMutI CAGTG 1 cut(s) 86
BtuMI TCGCGA 1 cut(s) 768
BveI ACCTGC 1 cut(s) 502
CaiI CAGNNNCTG 1 cut(s) 422
CfoI GCGC 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 686
CseI GACGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 621
CviAII CATG 3 cut(s) 554, 590, 711
CviJI RGCY 9 cut(s) 49, 156, 290, 316, 542, 731, 911, 918, 938
CviKI_1 RGCY 9 cut(s) 49, 156, 290, 316, 542, 731, 911, 918, 938
CviQI GTAC 1 cut(s) 621
DdeI CTNAG 4 cut(s) 150, 204, 501, 758
DpnI GATC 7 cut(s) 8, 393, 615, 829, 857, 943, 949
DpnII GATC 7 cut(s) 6, 391, 613, 827, 855, 941, 947
DriI GACNNNNNGTC 1 cut(s) 642
Eam1104I CTCTTC 4 cut(s) 47, 65, 218, 377
Eam1105I GACNNNNNGTC 1 cut(s) 642
EarI CTCTTC 4 cut(s) 47, 65, 218, 377
Ecl136II GAGCTC 1 cut(s) 731
Eco130I CCWWGG 1 cut(s) 589
Eco24I GRGCYC 1 cut(s) 733
Eco47I GGWCC 1 cut(s) 686
Eco53kI GAGCTC 1 cut(s) 731
Eco57I CTGAAG 2 cut(s) 71, 398
Eco88I CYCGRG 1 cut(s) 468
EcoICRI GAGCTC 1 cut(s) 731
EcoRI GAATTC 1 cut(s) 675
EcoT14I CCWWGG 1 cut(s) 589
EcoT38I GRGCYC 1 cut(s) 733
ErhI CCWWGG 1 cut(s) 589
FaeI CATG 3 cut(s) 557, 593, 714
FaiI YATR 7 cut(s) 11, 353, 531, 555, 591, 665, 712
FatI CATG 3 cut(s) 553, 589, 710
FauI CCCGC 2 cut(s) 772, 950
FbaI TGATCA 1 cut(s) 391
FblI GTMKAC 2 cut(s) 255, 530
Fnu4HI GCNGC 1 cut(s) 746
FokI GGATG 2 cut(s) 606, 693
FriOI GRGCYC 1 cut(s) 733
Fsp4HI GCNGC 1 cut(s) 746
FspBI CTAG 1 cut(s) 939
GlaI GCGC 1 cut(s) 19
GluI GCNGC 1 cut(s) 746
HapII CCGG 2 cut(s) 896, 908
HgaI GACGC 1 cut(s) 85
HhaI GCGC 1 cut(s) 20
Hin1II CATG 3 cut(s) 557, 593, 714
Hin6I GCGC 1 cut(s) 18
HinP1I GCGC 1 cut(s) 18
HincII GTYRAC 2 cut(s) 42, 256
HindII GTYRAC 2 cut(s) 42, 256
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 9 cut(s) 236, 419, 455, 550, 798, 823, 878, 892, 902
HpaII CCGG 2 cut(s) 896, 908
HphI GGTGA 2 cut(s) 517, 748
Hpy166II GTNNAC 5 cut(s) 42, 256, 531, 695, 755
Hpy8I GTNNAC 5 cut(s) 42, 256, 531, 695, 755
Hpy99I CGWCG 1 cut(s) 773
HpyAV CCTTC 2 cut(s) 95, 349
HpyCH4III ACNGT 3 cut(s) 39, 231, 498
HpyCH4IV ACGT 1 cut(s) 751
HpyCH4V TGCA 2 cut(s) 511, 748
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 341, 666
HpyF3I CTNAG 4 cut(s) 150, 204, 501, 758
HpySE526I ACGT 1 cut(s) 751
Hsp92II CATG 3 cut(s) 557, 593, 714
HspAI GCGC 1 cut(s) 18
Kpn2I TCCGGA 1 cut(s) 895
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 7 cut(s) 6, 391, 613, 827, 855, 941, 947
LmnI GCTCC 2 cut(s) 125, 728
Lsp1109I GCAGC 1 cut(s) 732
LweI GCATC 3 cut(s) 29, 498, 944
MaeI CTAG 1 cut(s) 939
MaeII ACGT 1 cut(s) 751
MalI GATC 7 cut(s) 8, 393, 615, 829, 857, 943, 949
MboI GATC 7 cut(s) 6, 391, 613, 827, 855, 941, 947
MflI RGATCY 1 cut(s) 941
MhlI GDGCHC 2 cut(s) 671, 733
MluCI AATT 8 cut(s) 520, 576, 603, 675, 832, 862, 870, 928
MlyI GAGTC 3 cut(s) 413, 544, 896
MmeI TCCRAC 2 cut(s) 213, 664
MroI TCCGGA 1 cut(s) 895
MroXI GAANNNNTTC 1 cut(s) 813
MseI TTAA 2 cut(s) 107, 285
MslI CAYNNNNRTG 1 cut(s) 594
MspI CCGG 2 cut(s) 896, 908
MvnI CGCG 1 cut(s) 768
MwoI GCNNNNNNNGC 3 cut(s) 296, 341, 666
NcoI CCATGG 1 cut(s) 589
NdeII GATC 7 cut(s) 6, 391, 613, 827, 855, 941, 947
NlaIII CATG 3 cut(s) 557, 593, 714
NruI TCGCGA 1 cut(s) 768
NspV TTCGAA 1 cut(s) 836
PcsI WCGNNNNNNNCGW 2 cut(s) 261, 823
PdmI GAANNNNTTC 1 cut(s) 813
PfeI GAWTC 6 cut(s) 236, 455, 798, 823, 878, 892
PflFI GACNNNGTC 1 cut(s) 690
PflMI CCANNNNNTGG 1 cut(s) 930
PkrI GCNGC 1 cut(s) 747
PleI GAGTC 3 cut(s) 413, 544, 896
PpsI GAGTC 3 cut(s) 413, 544, 896
Psp124BI GAGCTC 1 cut(s) 733
PspPI GGNCC 1 cut(s) 686
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 1 cut(s) 941
PsyI GACNNNGTC 1 cut(s) 690
RruI TCGCGA 1 cut(s) 768
RsaI GTAC 1 cut(s) 622
RsaNI GTAC 1 cut(s) 621
RseI CAYNNNNRTG 1 cut(s) 594
SacI GAGCTC 1 cut(s) 733
SalI GTCGAC 1 cut(s) 254
SaqAI TTAA 2 cut(s) 107, 285
SatI GCNGC 1 cut(s) 746
Sau3AI GATC 7 cut(s) 6, 391, 613, 827, 855, 941, 947
Sau96I GGNCC 1 cut(s) 686
SchI GAGTC 3 cut(s) 413, 544, 896
SduI GDGCHC 2 cut(s) 671, 733
SfaNI GCATC 3 cut(s) 29, 498, 944
SfuI TTCGAA 1 cut(s) 836
SinI GGWCC 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 2 cut(s) 157, 272
SmoI CTYRAG 2 cut(s) 157, 272
Sse9I AATT 8 cut(s) 520, 576, 603, 675, 832, 862, 870, 928
SsiI CCGC 2 cut(s) 779, 957
SspMI CTAG 1 cut(s) 939
SstI GAGCTC 1 cut(s) 733
StyI CCWWGG 1 cut(s) 589
TaaI ACNGT 3 cut(s) 39, 231, 498
TaiI ACGT 1 cut(s) 754
TaqI TCGA 7 cut(s) 32, 255, 293, 817, 826, 836, 854
TasI AATT 8 cut(s) 520, 576, 603, 675, 832, 862, 870, 928
TfiI GAWTC 6 cut(s) 236, 455, 798, 823, 878, 892
Tru1I TTAA 2 cut(s) 107, 285
Tru9I TTAA 2 cut(s) 107, 285
TscAI CASTG 1 cut(s) 86
TseI GCWGC 1 cut(s) 745
TspDTI ATGAA 5 cut(s) 17, 230, 236, 368, 444
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 1 cut(s) 86
Tth111I GACNNNGTC 1 cut(s) 690
Van91I CCANNNNNTGG 1 cut(s) 930
VpaK11BI GGWCC 1 cut(s) 686
XapI RAATTY 4 cut(s) 520, 675, 832, 862
XmiI GTMKAC 2 cut(s) 255, 530
XmnI GAANNNNTTC 1 cut(s) 813
XspI CTAG 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.