RchiOBHm_Chr3g0494601

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0009494
Physical Location & Seq
Forward (+)
43402683 .. 43411213
8531 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4902 bp
ATGGGCAAAGCTGTCATCTCCGACGACGAGGATGAGGTCGAGCTCGACGAGGACGATAGGGAACCCCTCGACGGGGACACAGTCGACCAGGGCGGCGGCGGCGACGAAGATGAGGACGAAGAAGACGAAGGGCCAGATGAATATGAGAAGGATGATTTTATCGTAGATGATGTTGATGAAGAAGATGAGGAAGAGCGGGCCGACAGTGATGAGGAGAGGCAGAAGAAGAAGAAAAGAAAGAAAAAAGAGGAGTATGTTCTTGATGAAGATGACTACGAGCTCCTGGAGGACAATAATGTGATTGCCCCGCGCAGAAAGGGAAAATTTAAGCGGCTGAAAAAAGCTCAGAGGCATGCTCAGGGGGACCTTGGTGGATTATCTGATGAGGAGGAGTTTCTTGGTACTGGTAAAGGGGGAAGAACTGCAGAGGAGAAGCTTAAGTTCTCATTATTTGGTGGTGATGATGAAGGCCCTCCACTTGAGGACATTGCTGAAGAGGAGGAACCAGCAGAAGCGGAAGATGACGGAGAAGATGAAATGGCAGACTTTATAGTCGACGAAGAATTTGATGAGGCTGGTGTTCCTGTCAGACAGAAGAAGCTTAAGAAAAAGAAATCCAGGCAGGCACCAGGAGTATCTTCATCTGCTCTGCAGGAAGCCCATGACATATTCGGTGATGTTGATGAATTACTGCAGCTGCGTAAGCAAGGTTTGGATTCTAGTGAATGGAAGGAAAGGAGGCTTGAAGATGAATTTGAACCAATTGTTCTTTCGGAAAAGTATATGACAGAGAAGGATGACCAGATTCGGGAGATTGATGTCCCAGAAAGAATTCAGGTATATGAGGAAAGCACTGGTTTTCCTCCACTTGATGAAAAGAGCATAGATGATGAGAGTACCTGGATATTCAATCAACTTGCAAGTGGTACAGTTCCTTTTTTTGGCAAGACAGGCCTGGGGAATTCCATAAGCAGGGACGACATCATTGGATTCTTGAACTTGCACCATGTGCAGAAATTGGATGTTCCTTTTATTGCGATGTATCGGAAAGAGGAGTGCCTTAGTGTATTGAAAGATCCTGAACACATGGACATGGATGATCAAAATGAGAAAGCATCCACACTAAAGTGGCACAAGGTACTTTGGTCCATTCAGGACTTGCATAGAAAATGGTTGCTGCTTCAGAAGAGGAAAAGTGCTCTGCAATCTTATTACGAAAAGCGGTTTGATGAAGAATCTCGACGCATATATGATGAGACCAGACTAACTTTAAATCAGCAACTTTTTGAATCAATTATGAAGTCACTAAAGGCTGCAGAATCAGAGAGAGAAGTTGATGATGTGGACACCAAGTTTAACTTGCATTTCCCTGCTGGTGAGATAGGTGTGGATGAGGGCCAATATAAGAGGCCTAAGAGGAAGTCCCTTTATAGTATCTGCAGTAAGGCTGGCTTGTGGGAGGTTGCCAGCAAGTTTGGTTACACCTCTGAACAGTTTGGTTTGCAATTGTCACTAGAGAAAATGAGAATGGATGAGCTGGAGGATGCGAAAGAGACGCCAGAGGAATTGGCTTCAAACTTCACATGTGCAATGTTTGAGACACCTCAGGAAGTGCTCAAAGGAGCCAGGCATATGGCTGCTGTTGAGATAAGCTGTGAGCCATGTGTCAGGAAGTATGTTAGGAGCAACTACTTGGATATGGTTGAGTTGTCCACCAGCCCAACACCAGACGGAAATACTGCAATTGAAGCTTTTCATCAGTTTGCAGGGGTGAAGTGGTTGCAAAGAAAGCCATTAAATAGATTTGGGGATGCACAATGGCTGCTTATCCAGAAGGCTGAAGAGGAGAAGCTTCTTCAAGTTACTATTAAGCTACCGGAAGACAGACTAAACAAGTTGATGAGCGACTTTAATGAGTACTATCTTAGTGATGGTGTTAGTAAATCTGCACAACTGTGGAATGAGCAGCGGAAGTTGATATTGCAGGATGCTCTTTTCAATTTTCTTCTACCTTCAATGGAGAAAGAAGCAAGATCCTTGTTGACTAGTAGGGCAAAAAACTGGTTATTGATGGAATACGGAAAGGTTTTGTGGAATAAAGTCTCTGTGGGACCATATCAACGGAAAGAGAATGACATTAGTTCAGACGATGAAGCTGCACCCAGGGTCATGGCTTGCTGTTGGGGCCCTGGGAAGCCAGCAACCACTTTTGTTATGTTAGATTCATCGGGGGAGGTGCTAGATGTGCTTTACACGGGATCTCTCACCTTACGCTCCCAGAACGTTAACGATCAGCAACGGAAGAAAAATGACCAAGAACGTGTTTTGAAGTTCATGACTGATCACCAACCACATGTGGCTGTTCTAGGAGCTGTTAATTTGTCTTGTGTTCGCCTGAAAGAAGATATTTACGAGATAATCTTTAAGATGGTGGAGGAGAATCCTAGAGATGTTGGACATGATATGGATGGGTTAAGCATTGTCTATGGAGATGAATCTTTGGCTCGCCTGTATGAAAACTCTCGCATTTCATCTGATCAGCTACCTGCACAATCAGGCATTGTGAAGCGTGCTGTTGCTCTGGGGCGTTACCTTCAAAATCCATTGGCAATGGTCGCCACTCTTTGTGGGCCAGGAAGAGAAATCTTATCTTGGAAACTCAATCCTTTGGAGAACTTTCTCACCCCAGATGAGAAGTATGGAATGATTGAACAAGTCATGGTAGATGTTACAAACCAAGTAGGCCTTGACATAAATTTGGCTATAAGCCACGAATGGTTATTTGCACCTTTGCAATTCATTTCTGGGCTCGGACCTAGAAAAGCAGCATTTTTGCAGCGATCGTTGGTAAGATCTGGTGCAATATTTACTCGGAAGGACTTTTTGACAGCACATGGGTTAAGTAAAAAGGTGTTTGTTAATGCAGTTGGTTTCTTGCGTGTTCGGCGCAGTGGGCTGGCTGCTAGCAGCAGCCAATTTATTGATCTGTTGGATGATACAAGAATTCATCCAGAATCCTATATTCTTGCACAAGAATTGGCCAAAGATGTTTTTGAAGTTGATGGTGGCAATGATGATGAGGATGCTATGGAGATGGCTATAGAACATGTTAGGGATCGGCCTGCTTATCTAAAAAATCTTGATGTTGAAGCGTATGCTAAAAGCAAAGAGCGTGAGAATAAGATACAAACATTTTGTGACATCAGGAGAGAATTAATACAGGGTTTTCAGGATTGGCGTAAGAAATATGAAGAACTAAGTCAAGATGATGAATTCTATATGATTTCAGGTGAAACTGAAGATACCCTTGCTGAAGGAAGAATTGTGCTGGCCACAGTTCGCAGGGTACAAGGTCAAAGGGCAATATGTGCACTTGAATCTGGACTGACTGGTATGCTTATGAAAGAAGACTATTCAGATGATTCAAGGGATATTTCTGACTTATCTGAGAGACTGAATGAGGGTGACATTCTCACGTGCAAGATCAAATCTATTCAAAAGAATAGGTATCAGGTGTTCCTAGTTTGTAGAGAGAGTGAAATGAGAAATAATCGAAATCAAAATATTCAGCCTCTCGATTCCTATTACCATGAAGACAGGAGAAGTTTACAGACAGAGCAAGAAAAAGCTCGTAAGGAGAAAGAGCTTGCGAAAAAACATTTCAAGCCAAGGATGATTGTTCATCCTCGCTTCCAGAATATTACGGCTGATGAAGCAATGAAGTTCTTATCTGATAAAGATCCTGGGGAAAGTATTATTCGTCCTAGTTCCCGTGGGCCTTCGTATTTGACCTTAACTCTGAAAGTTTACGATGGAGTTTATGCTCACAAAGACGTAGTTGAAGGTGGAAAGGAACACAAGGATATCACTAGCTTACTCCGTATTGGAAAGACTCTGAAAATTGGAGAGGACACTTTTGAAGATTTAGATGAGGTAATGGATCGTTATGTTGATCCCCTGGTGTCTCACTTGAAGGCAATGCTAAATTATCGCAAATTCCGACGGGGCACAAAAGCAGAGGTTGATGAACTGTTGAAGATTGAAAAATTGGAGTTTCCTATGAGGATTGTTTATTGTTTCGGGATATCACATGAACATCCAGGGACTTTCATACTGACCTATATACGGAGTACAAATCCACACCATGAGTATGTTGGGCTGTATCCTAAGGGGTTCAAGTTCAGAAAAAGGATGTTTGAGAGCATTGATCGGCTTGTAGCACATTTTCAGAAGTATATTGATGAGCCTCAACATGAGTCCGGACCATCAATTAGATCAGTTGCTGCTATGGTGCCCATGCGAAGCCCTGCCACTGGGGGTTCTTCGGGTGCATCCGCAGGATGGGGTGGTTCAAACAATGAGGGTGGTTGGAGAGGCCAGTCGTTTGACAGGGATAGGTCTTCTACACCGAGTTCGCGAACAGGCAGAAATGATTACAGAAATGGTGGCGGTCGAGATGGACATCCAAGTGGCCTACCTAGACCATATGGGGGGCGCGGTCGAGGTCGAGGAACATATAACGATAGCCGAGGCAATAATGAGAGACATGATTCAGGTTTTGATGGTCCTACATGGGGTTCAGATACCAAGGATGGCAATGATGGCTTGGGCAACTTTCCAGGTGCCAAGGTTCAAAACTCACCTGGCAGGGAAGCTTTCCCAGGTGGCTGGGGTACAGGTGGAAGTGGTAGTGGAGGAGGCAGCAGCTGGGCTGGTGGTGGCGGAAGTGGCTGGGGTCAAGAAAGTGGTGGTGGCTCAGGAGGATGGGGTGGGGCCGCTGCAAATGATGCTGCTGCTGATAATGAGCCTTCAGGATGGGGTTCTGACTCGAAAAAGAAGGGTTCGGGTGATGGTACTTCAGGATGGGGTTCTCAGAGTGGAGGTGGCGGTGGTGGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

1633

Amino Acids

184.14

Weight (kDa)

5.02

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT6_acidic PF14632 35 - 117 6.6e-17 Acidic N-terminal SPT6
HTH_44 PF14641 290 - 407 1.5e-06 Helix-turn-helix DNA-binding domain of SPT6
Tex_central_region PF22706 516 - 685 1.1e-06 Tex central region-like
YqgF PF14639 720 - 876 4.2e-17 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 880 - 981 2.1e-20 Helix-hairpin-helix motif
Spt6_S1 PF21710 1092 - 1180 2.5e-08 Spt6, S1/OB domain
SH2_2 PF14633 1196 - 1411 6.8e-69 SH2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 551
Acc36I ACCTGC 1 cut(s) 2554
AccB1I GGYRCC 3 cut(s) 625, 4291, 4622
AccBSI CCGCTC 1 cut(s) 196
AccI GTMKAC 2 cut(s) 84, 555
AccII CGCG 3 cut(s) 310, 4417, 4497
AccIII TCCGGA 1 cut(s) 4259
AclI AACGTT 1 cut(s) 2283
AclWI GGATC 7 cut(s) 1070, 2028, 2266, 3123, 3734, 3947, 3948
AcoI YGGCCR 2 cut(s) 3039, 3330
AcsI RAATTY 9 cut(s) 323, 563, 752, 831, 961, 2755, 3003, 3272, 3995
AcuI CTGAAG 7 cut(s) 513, 1166, 1860, 3318, 3333, 4794, 4842
AcvI CACGTG 1 cut(s) 3477
AcyI GRCGYC 1 cut(s) 1556
AdeI CACNNNGTG 1 cut(s) 1009
AfaI GTAC 9 cut(s) 403, 898, 928, 1140, 1919, 3348, 4132, 4675, 4855
AflII CTTAAG 2 cut(s) 437, 602
AflIII ACRYGT 4 cut(s) 1583, 2320, 2353, 3106
AhlI ACTAGT 1 cut(s) 2045
AjuI GAANNNNNNNTTGG 2 cut(s) 754, 786
AleI CACNNNNGTG 2 cut(s) 1126, 1954
Alw21I GWGCWC 5 cut(s) 45, 282, 1201, 1617, 3373
Alw26I GTCTC 7 cut(s) 1250, 1547, 1592, 2107, 3445, 3969, 4537
Alw44I GTGCAC 1 cut(s) 3369
AlwI GGATC 7 cut(s) 1070, 2028, 2266, 3123, 3734, 3947, 3948
AlwNI CAGNNNCTG 2 cut(s) 4283, 4707
Aor13HI TCCGGA 1 cut(s) 4259
ApaI GGGCCC 1 cut(s) 2189
ApaLI GTGCAC 1 cut(s) 3369
ApoI RAATTY 9 cut(s) 323, 563, 752, 831, 961, 2755, 3003, 3272, 3995
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
AseI ATTAAT 1 cut(s) 3215
Asp700I GAANNNNTTC 2 cut(s) 831, 1752
AspLEI GCGC 3 cut(s) 312, 2949, 4497
AsuNHI GCTAGC 1 cut(s) 2963
AvaII GGWCC 6 cut(s) 364, 1146, 2111, 2813, 4262, 4565
AxyI CCTNAGG 2 cut(s) 1605, 4167
BaeGI GKGCMC 4 cut(s) 2189, 3373, 4010, 4296
BaeI ACNNNNGTAYC 2 cut(s) 1130, 1163
BalI TGGCCA 2 cut(s) 3041, 3332
BanI GGYRCC 3 cut(s) 625, 4291, 4622
BanII GRGCYC 4 cut(s) 45, 282, 2189, 2811
BbrPI CACGTG 1 cut(s) 3477
BbsI GAAGAC 5 cut(s) 129, 1887, 3414, 3600, 4392
Bbv12I GWGCWC 5 cut(s) 45, 282, 1201, 1617, 3373
BceAI ACGGC 1 cut(s) 3720
BcgI CGANNNNNNTGC 2 cut(s) 2138, 2172
BciVI GTATCC 1 cut(s) 4173
BclI TGATCA 3 cut(s) 1099, 2341, 2536
BcoDI GTCTC 7 cut(s) 1250, 1547, 1592, 2107, 3445, 3969, 4537
BcuI ACTAGT 1 cut(s) 2045
BfmI CTRYAG 6 cut(s) 423, 650, 692, 1314, 1438, 3099
BfrI CTTAAG 2 cut(s) 437, 602
BfuAI ACCTGC 1 cut(s) 2554
BfuI GTATCC 1 cut(s) 4173
BglII AGATCT 1 cut(s) 2852
BmcAI AGTACT 1 cut(s) 1919
Bme18I GGWCC 6 cut(s) 364, 1146, 2111, 2813, 4262, 4565
BmrI ACTGGG 1 cut(s) 4323
BmsI GCATC 7 cut(s) 1124, 1534, 1801, 1978, 3073, 4340, 4777
BmtI GCTAGC 1 cut(s) 2967
BmuI ACTGGG 1 cut(s) 4323
BpiI GAAGAC 5 cut(s) 129, 1887, 3414, 3600, 4392
BplI GAGNNNNNCTC 2 cut(s) 340, 372
BpmI CTGGAG 2 cut(s) 305, 1559
Bpu10I CCTNAGC 2 cut(s) 357, 4756
BpuEI CTTGAG 1 cut(s) 500
BsaAI YACGTR 1 cut(s) 3477
BsaBI GATNNNNATC 2 cut(s) 3738, 4461
BsaHI GRCGYC 1 cut(s) 1556
BsaI GGTCTC 1 cut(s) 1250
BsaWI WCCGGW 2 cut(s) 1876, 4259
Bse1I ACTGG 6 cut(s) 409, 859, 2066, 3394, 4318, 4378
Bse21I CCTNAGG 2 cut(s) 1605, 4167
Bse3DI GCAATG 7 cut(s) 486, 1596, 2616, 3076, 3723, 3984, 4603
Bse8I GATNNNNATC 2 cut(s) 3738, 4461
BseAI TCCGGA 1 cut(s) 4259
BseJI GATNNNNATC 2 cut(s) 3738, 4461
BseMI GCAATG 7 cut(s) 486, 1596, 2616, 3076, 3723, 3984, 4603
BseMII CTCAG 6 cut(s) 359, 371, 1619, 3438, 4770, 4886
BseNI ACTGG 6 cut(s) 409, 859, 2066, 3394, 4318, 4378
BseSI GKGCMC 4 cut(s) 2189, 3373, 4010, 4296
BseYI CCCAGC 3 cut(s) 4668, 4707, 4731
BsgI GTGCAG 4 cut(s) 1031, 1932, 2142, 2532
Bsh1236I CGCG 3 cut(s) 310, 4417, 4497
Bsh1285I CGRYCG 3 cut(s) 2843, 4453, 4501
BshNI GGYRCC 3 cut(s) 625, 4291, 4622
BsiEI CGRYCG 3 cut(s) 2843, 4453, 4501
BsiHKAI GWGCWC 5 cut(s) 45, 282, 1201, 1617, 3373
BsiSI CCGG 2 cut(s) 1877, 4260
BslFI GGGAC 7 cut(s) 89, 377, 806, 989, 1408, 2124, 4117
BsmAI GTCTC 7 cut(s) 1250, 1547, 1592, 2107, 3445, 3969, 4537
BsmBI CGTCTC 1 cut(s) 1547
BsmFI GGGAC 7 cut(s) 89, 377, 806, 989, 1408, 2124, 4117
Bso31I GGTCTC 1 cut(s) 1250
Bsp120I GGGCCC 1 cut(s) 2185
Bsp1286I GDGCHC 9 cut(s) 45, 282, 1201, 1617, 2189, 2811, 3373, 4010, 4296
Bsp13I TCCGGA 1 cut(s) 4259
Bsp68I TCGCGA 1 cut(s) 4417
BspCNI CTCAG 6 cut(s) 358, 370, 1618, 3439, 4769, 4885
BspEI TCCGGA 1 cut(s) 4259
BspFNI CGCG 3 cut(s) 310, 4417, 4497
BspHI TCATGA 1 cut(s) 2334
BspMAI CTGCAG 5 cut(s) 427, 654, 696, 1318, 1442
BspMI ACCTGC 1 cut(s) 2554
BspOI GCTAGC 1 cut(s) 2967
BspPI GGATC 7 cut(s) 1070, 2028, 2266, 3123, 3734, 3947, 3948
BspQI GCTCTTC 1 cut(s) 186
BspT107I GGYRCC 3 cut(s) 625, 4291, 4622
BspTI CTTAAG 2 cut(s) 437, 602
BspTNI GGTCTC 1 cut(s) 1250
BsrBI CCGCTC 1 cut(s) 196
BsrDI GCAATG 7 cut(s) 486, 1596, 2616, 3076, 3723, 3984, 4603
BsrI ACTGG 6 cut(s) 409, 859, 2066, 3394, 4318, 4378
BssNI GRCGYC 1 cut(s) 1556
BssT1I CCWWGG 4 cut(s) 367, 3668, 4587, 4626
Bst4CI ACNGT 7 cut(s) 82, 206, 931, 1494, 1956, 3337, 4032
Bst6I CTCTTC 5 cut(s) 186, 489, 1181, 1836, 2632
BstACI GRCGYC 1 cut(s) 1556
BstAFI CTTAAG 2 cut(s) 437, 602
BstAPI GCANNNNNTGC 3 cut(s) 1009, 3368, 4787
BstBAI YACGTR 1 cut(s) 3477
BstDSI CCRYGG 1 cut(s) 3772
BstFNI CGCG 3 cut(s) 310, 4417, 4497
BstHHI GCGC 3 cut(s) 312, 2949, 4497
BstMAI GTCTC 7 cut(s) 1250, 1547, 1592, 2107, 3445, 3969, 4537
BstMCI CGRYCG 3 cut(s) 2843, 4453, 4501
BstNSI RCATGY 4 cut(s) 356, 1587, 2357, 3110
BstSFI CTRYAG 6 cut(s) 423, 650, 692, 1314, 1438, 3099
BstSLI GKGCMC 4 cut(s) 2189, 3373, 4010, 4296
BstUI CGCG 3 cut(s) 310, 4417, 4497
BstV2I GAAGAC 5 cut(s) 129, 1887, 3414, 3600, 4392
BstX2I RGATCY 5 cut(s) 1075, 2033, 2258, 2852, 3739
BstXI CCANNNNNNTGG 3 cut(s) 1633, 2170, 4668
BstYI RGATCY 5 cut(s) 1075, 2033, 2258, 2852, 3739
Bsu36I CCTNAGG 2 cut(s) 1605, 4167
BsuI GTATCC 1 cut(s) 4173
BtgI CCRYGG 1 cut(s) 3772
BtgZI GCGATG 1 cut(s) 1052
BtsI GCAGTG 1 cut(s) 2956
BtsIMutI CAGTG 4 cut(s) 211, 852, 2956, 4311
BtuMI TCGCGA 1 cut(s) 4417
BveI ACCTGC 1 cut(s) 2554
CaiI CAGNNNCTG 2 cut(s) 4283, 4707
CciI TCATGA 1 cut(s) 2334
CfoI GCGC 3 cut(s) 312, 2949, 4497
CseI GACGC 2 cut(s) 1251, 1564
Csp6I GTAC 9 cut(s) 402, 897, 927, 1139, 1918, 3347, 4131, 4674, 4854
CspCI CAANNNNNGTGG 2 cut(s) 4345, 4380
CviQI GTAC 9 cut(s) 402, 897, 927, 1139, 1918, 3347, 4131, 4674, 4854
DraI TTTAAA 1 cut(s) 1272
DraIII CACNNNGTG 1 cut(s) 1009
DrdI GACNNNNNNGTC 1 cut(s) 551
DseDI GACNNNNNNGTC 1 cut(s) 551
EaeI YGGCCR 2 cut(s) 3039, 3330
Eam1104I CTCTTC 5 cut(s) 186, 489, 1181, 1836, 2632
EarI CTCTTC 5 cut(s) 186, 489, 1181, 1836, 2632
EciI GGCGGA 1 cut(s) 4737
Ecl136II GAGCTC 2 cut(s) 43, 280
Eco130I CCWWGG 4 cut(s) 367, 3668, 4587, 4626
Eco147I AGGCCT 3 cut(s) 954, 1411, 2745
Eco24I GRGCYC 4 cut(s) 45, 282, 2189, 2811
Eco31I GGTCTC 1 cut(s) 1250
Eco32I GATATC 2 cut(s) 3865, 4086
Eco47I GGWCC 6 cut(s) 364, 1146, 2111, 2813, 4262, 4565
Eco53kI GAGCTC 2 cut(s) 43, 280
Eco57I CTGAAG 7 cut(s) 513, 1166, 1860, 3318, 3333, 4794, 4842
Eco72I CACGTG 1 cut(s) 3477
Eco81I CCTNAGG 2 cut(s) 1605, 4167
EcoICRI GAGCTC 2 cut(s) 43, 280
EcoO109I RGGNCCY 4 cut(s) 364, 470, 2185, 2186
EcoRI GAATTC 4 cut(s) 831, 961, 3003, 3272
EcoRV GATATC 2 cut(s) 3865, 4086
EcoT14I CCWWGG 4 cut(s) 367, 3668, 4587, 4626
EcoT38I GRGCYC 4 cut(s) 45, 282, 2189, 2811
ErhI CCWWGG 4 cut(s) 367, 3668, 4587, 4626
Esp3I CGTCTC 1 cut(s) 1547
FalI AAGNNNNNCTT 8 cut(s) 1343, 1375, 1436, 1468, 2730, 2762, 3291, 3323
FaqI GGGAC 7 cut(s) 89, 377, 806, 989, 1408, 2124, 4117
FauI CCCGC 2 cut(s) 189, 315
FauNDI CATATG 2 cut(s) 1632, 4486
FbaI TGATCA 3 cut(s) 1099, 2341, 2536
FblI GTMKAC 2 cut(s) 84, 555
FriOI GRGCYC 4 cut(s) 45, 282, 2189, 2811
GlaI GCGC 3 cut(s) 311, 2948, 4496
GsaI CCCAGC 3 cut(s) 4672, 4711, 4735
GsuI CTGGAG 2 cut(s) 305, 1559
HapII CCGG 2 cut(s) 1877, 4260
HgaI GACGC 2 cut(s) 1251, 1564
HhaI GCGC 3 cut(s) 312, 2949, 4497
Hin1I GRCGYC 1 cut(s) 1556
Hin6I GCGC 3 cut(s) 310, 2947, 4495
HinP1I GCGC 3 cut(s) 310, 2947, 4495
HincII GTYRAC 4 cut(s) 85, 556, 2043, 2287
HindII GTYRAC 4 cut(s) 85, 556, 2043, 2287
HindIII AAGCTT 5 cut(s) 434, 599, 1749, 1850, 4653
HpaI GTTAAC 1 cut(s) 2287
HpaII CCGG 2 cut(s) 1877, 4260
Hpy166II GTNNAC 9 cut(s) 85, 556, 1345, 1713, 2043, 2287, 3371, 3608, 3808
Hpy8I GTNNAC 9 cut(s) 85, 556, 1345, 1713, 2043, 2287, 3371, 3608, 3808
Hpy99I CGWCG 8 cut(s) 26, 29, 50, 74, 107, 560, 1245, 4005
HpyCH4III ACNGT 7 cut(s) 82, 206, 931, 1494, 1956, 3337, 4032
HpyCH4IV ACGT 4 cut(s) 2283, 2320, 3476, 3834
HpySE526I ACGT 4 cut(s) 2283, 2320, 3476, 3834
Hsp92I GRCGYC 1 cut(s) 1556
HspAI GCGC 3 cut(s) 310, 2947, 4495
Kpn2I TCCGGA 1 cut(s) 4259
Ksp22I TGATCA 3 cut(s) 1099, 2341, 2536
KspAI GTTAAC 1 cut(s) 2287
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 5 cut(s) 285, 1622, 1683, 2279, 2369
LweI GCATC 7 cut(s) 1124, 1534, 1801, 1978, 3073, 4340, 4777
MaeII ACGT 4 cut(s) 2283, 2320, 3476, 3834
MaeIII GTNAC 8 cut(s) 1302, 1478, 1509, 1861, 2588, 2728, 3197, 3464
MbiI CCGCTC 1 cut(s) 196
MfeI CAATTG 3 cut(s) 762, 1505, 1743
MflI RGATCY 5 cut(s) 1075, 2033, 2258, 2852, 3739
MhlI GDGCHC 9 cut(s) 45, 282, 1201, 1617, 2189, 2811, 3373, 4010, 4296
MlsI TGGCCA 2 cut(s) 3041, 3332
MluNI TGGCCA 2 cut(s) 3041, 3332
MlyI GAGTC 3 cut(s) 3886, 4265, 4820
MmeI TCCRAC 5 cut(s) 45, 2434, 2970, 4024, 4349
Mox20I TGGCCA 2 cut(s) 3041, 3332
MroI TCCGGA 1 cut(s) 4259
MroXI GAANNNNTTC 2 cut(s) 831, 1752
MscI TGGCCA 2 cut(s) 3041, 3332
MslI CAYNNNNRTG 5 cut(s) 1091, 1126, 1954, 4149, 4467
Msp20I TGGCCA 2 cut(s) 3041, 3332
MspA1I CMGCKG 4 cut(s) 697, 1969, 4707, 4778
MspCI CTTAAG 2 cut(s) 437, 602
MspI CCGG 2 cut(s) 1877, 4260
MunI CAATTG 3 cut(s) 762, 1505, 1743
MvnI CGCG 3 cut(s) 310, 4417, 4497
NdeI CATATG 2 cut(s) 1632, 4486
NheI GCTAGC 1 cut(s) 2963
NmeAIII GCCGAG 1 cut(s) 4553
NmuCI GTSAC 4 cut(s) 1302, 1509, 3197, 3464
NruI TCGCGA 1 cut(s) 4417
NspI RCATGY 4 cut(s) 356, 1587, 2357, 3110
OliI CACNNNNGTG 2 cut(s) 1126, 1954
PaeI GCATGC 1 cut(s) 356
PagI TCATGA 1 cut(s) 2334
PasI CCCWGGG 2 cut(s) 2163, 2189
PceI AGGCCT 3 cut(s) 954, 1411, 2745
PciI ACATGT 3 cut(s) 1583, 2353, 3106
PciSI GCTCTTC 1 cut(s) 186
PcsI WCGNNNNNNNCGW 4 cut(s) 45, 51, 123, 3997
PdmI GAANNNNTTC 2 cut(s) 831, 1752
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 282
Ple19I CGATCG 1 cut(s) 2843
PleI GAGTC 3 cut(s) 3886, 4264, 4820
PmaCI CACGTG 1 cut(s) 3477
PmlI CACGTG 1 cut(s) 3477
PpsI GAGTC 3 cut(s) 3886, 4264, 4820
Ppu21I YACGTR 1 cut(s) 3477
PpuMI RGGWCCY 1 cut(s) 364
PscI ACATGT 3 cut(s) 1583, 2353, 3106
PshBI ATTAAT 1 cut(s) 3215
Psp124BI GAGCTC 2 cut(s) 45, 282
Psp1406I AACGTT 1 cut(s) 2283
Psp5II RGGWCCY 1 cut(s) 364
PspCI CACGTG 1 cut(s) 3477
PspFI CCCAGC 3 cut(s) 4668, 4707, 4731
PspOMI GGGCCC 1 cut(s) 2185
PspPPI RGGWCCY 1 cut(s) 364
PsrI GAACNNNNNNTAC 2 cut(s) 3500, 3532
PstI CTGCAG 5 cut(s) 427, 654, 696, 1318, 1442
PstNI CAGNNNCTG 2 cut(s) 4283, 4707
PsuI RGATCY 5 cut(s) 1075, 2033, 2258, 2852, 3739
PsyI GACNNNGTC 1 cut(s) 80
PvuI CGATCG 1 cut(s) 2843
PvuII CAGCTG 2 cut(s) 697, 4707
RruI TCGCGA 1 cut(s) 4417
RsaI GTAC 9 cut(s) 403, 898, 928, 1140, 1919, 3348, 4132, 4675, 4855
RsaNI GTAC 9 cut(s) 402, 897, 927, 1139, 1918, 3347, 4131, 4674, 4854
RseI CAYNNNNRTG 5 cut(s) 1091, 1126, 1954, 4149, 4467
SacI GAGCTC 2 cut(s) 45, 282
SalI GTCGAC 2 cut(s) 83, 554
SapI GCTCTTC 1 cut(s) 186
ScaI AGTACT 1 cut(s) 1919
SchI GAGTC 3 cut(s) 3886, 4265, 4820
SduI GDGCHC 9 cut(s) 45, 282, 1201, 1617, 2189, 2811, 3373, 4010, 4296
SfaNI GCATC 7 cut(s) 1124, 1534, 1801, 1978, 3073, 4340, 4777
SfcI CTRYAG 6 cut(s) 423, 650, 692, 1314, 1438, 3099
SinI GGWCC 6 cut(s) 364, 1146, 2111, 2813, 4262, 4565
SmiMI CAYNNNNRTG 5 cut(s) 1091, 1126, 1954, 4149, 4467
SmlI CTYRAG 3 cut(s) 437, 479, 602
SmoI CTYRAG 3 cut(s) 437, 479, 602
SpeI ACTAGT 1 cut(s) 2045
SphI GCATGC 1 cut(s) 356
SseBI AGGCCT 3 cut(s) 954, 1411, 2745
SspI AATATT 3 cut(s) 2865, 3565, 3700
SstI GAGCTC 2 cut(s) 45, 282
StuI AGGCCT 3 cut(s) 954, 1411, 2745
StyI CCWWGG 4 cut(s) 367, 3668, 4587, 4626
TaaI ACNGT 7 cut(s) 82, 206, 931, 1494, 1956, 3337, 4032
TaiI ACGT 4 cut(s) 2286, 2323, 3479, 3837
TatI WGTACW 2 cut(s) 1917, 4130
TauI GCSGC 5 cut(s) 96, 99, 102, 334, 4778
TscAI CASTG 4 cut(s) 211, 859, 2956, 4318
TseFI GTSAC 4 cut(s) 1302, 1509, 3197, 3464
Tsp45I GTSAC 4 cut(s) 1302, 1509, 3197, 3464
TspGWI ACGGA 7 cut(s) 540, 1746, 2094, 2137, 2314, 3869, 4141
TspRI CASTG 4 cut(s) 211, 859, 2956, 4318
Tth111I GACNNNGTC 1 cut(s) 80
Vha464I CTTAAG 2 cut(s) 437, 602
VneI GTGCAC 1 cut(s) 3369
VpaK11BI GGWCC 6 cut(s) 364, 1146, 2111, 2813, 4262, 4565
VspI ATTAAT 1 cut(s) 3215
XapI RAATTY 9 cut(s) 323, 563, 752, 831, 961, 2755, 3003, 3272, 3995
XceI RCATGY 4 cut(s) 356, 1587, 2357, 3110
XcmI CCANNNNNNNNNTGG 1 cut(s) 4151
XmiI GTMKAC 2 cut(s) 84, 555
XmnI GAANNNNTTC 2 cut(s) 831, 1752
ZrmI AGTACT 1 cut(s) 1919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.