Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0009864

Physical Location & Seq

Genomic Coordinates Reverse (-)

444036 .. 448580

Gene Structure 4545 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1467 bp

ATGGCCATGGCAACTATTTCAGCTCCACCCCATCCCTCCTCAACGTTTCACTCTCCCAAACAGCTGTCTTCCTCTTCCGGCAGCCTCTGCTTTTCCTTGTCTGAAACTCTTACCTTCCCTTCATCATCATCATCGGCTTCGAGATCGATAAGTCACAACAAACTCACCCTCTACGCTACTAGAAGCATCCACCAGAACATCTCCAATAACGGCTCTCCCCCTCCTCCAACCCCCGGCGCCAAGGGAGTGAGGAGGCATGCTATTTCAGTGTTCGTTGGGGACGAAAGCGGAATGATTAATCGGATTTCAGGGGTGTTTGCAAGGCGGGGTTACAACATCGAATCTCTGGCTGTTGGTTTGAATAAGGACAAAGCCCTCTTCACCATTGTCGTTTCTGGCACCGATAATGTCTTGCGCCAAGTCATTGAGCAGCTTAACAAGCTCGTCAATGTTATCAAGGTTGAGGATCTCTCTAATGAGCCTCAAGTAGAACGTGAACTAATGCTTATAAAAGTAAATGCGGATCCGAGTTACCTTGCTGAGATCAAGTGGTTGGTGAATATATTTAGAGCAAAAATTGTGGATATCTCAGACTACTCAGTCACCATTGAGGTGACAGGAGATCCTGGGAAAATGGTTGCTCTGCAAAGAAATTTAAGCAAGTTTGGGATCAGAGAAATTGCCAGAACCGGAAAGATAGCTTTGAGGAGAGAAAAGTTGGGTGCTTCCGCTCCATTTTGGCGCTATTCAGCAGCTTCATATCCAGATCTTGAGAGAACAGTGACTGTTGATGCTCTTGCAGGAGTTGAGAAGGGAGTTGTTAACGCCGAATCTGATACATCTTCTGGGGGTGATGTTTATCCTGTGGAGCCTTCTGGTACCGTTACTGTCAATCAAGTCCTTAATGCTAACTGGGGTGCTCTTAATGATGATGATACAAGTGGACTTCGATCGCACACTTTATCTATGCTTGTAAATGATGTTCCTGGGGTTCTTTACATTATTACGGGCGTTTTTGCTCGAAGGGGTTATAACATTCAGAGTTTAGCTGTTGGCCATGCAGAAGTTGAGGGGCAATCTCGCATTACTACTGTTGTTCCTGGTACAGATGAATCGATTAGCAAGTTGGTGCAACAGCTCTATAAGCTTGTCGATGTGCATGACGTTCGAGATCTTACCAACTTGCCATTTGCTGAGCGGGAATTGATGTTGATAAAAATTGCTGCCAATGCTGTTGCTCGGAGAGATGTACTTGACATTGGAAACATCTTTAGGGCCAAGGCGGTTGATGTTTCTGACCACACTATAACCCTTGAGCTCACAGGAGACCTGAACAAGATGGTTGCACTGCAGAGATTGTTAGAACCCTATGGGATTTGTGAGGTGGCGCGTACTGGAAGAATTGCTCTGGTGCGTGAGTCAGGGGTTGATTCTAGATATCTCCGTGGATATTCTTTACCTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0005948 GO:0006082 GO:0006520 GO:0006549 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009097 GO:0009099 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0032991 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043621 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0050790 GO:0065007 GO:0065009 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

ACT ACT_5 ALS_ss_C

Protein Analysis

488

Amino Acids

53.0

Weight (kDa)

7.77

Isoelectric Point (pI)

44.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACT	PF01842	86 - 149	6.7e-10	ACT domain
ACT_AHAS_ss	PF22629	88 - 153	4.5e-17	AHAS small subunit-like ACT domain
ACT_5	PF13710	96 - 154	2.1e-07	ACT domain
ALS_ss_C	PF10369	165 - 238	2.6e-27	Small subunit of acetolactate synthase
ACT	PF01842	319 - 383	1.5e-08	ACT domain
ACT_AHAS_ss	PF22629	321 - 387	8.9e-22	AHAS small subunit-like ACT domain
ACT_5	PF13710	328 - 389	1.8e-10	ACT domain
ALS_ss_C	PF10369	400 - 472	2.6e-26	Small subunit of acetolactate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0009864)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31810 AT2G31810 AT2G31810
fragaria_vesca	FvH4_1g00350 FvH4_1g00350
malus_domestica	MD02G1002700.v1.1 MD15G1145900.v1.1
prunus_persica	Prupe.7G268500_v2.0.a1
pyrus_communis	pycom15g13110
rosa_chinensis	RchiOBHm_Chr2g0084781
rosa_laevigata	RLG00000015638
rosa_multiflora	Rmu_sc0002352.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155820
rosa_rugosa	Rorug01G0456000 Rorug01G0456100 Rorug01G0456200
rosa_samantha	Rh2AG003300 Rh2BG004900 Rh2CG005300 Rh2DG004700
rosa_wichuraiana	Rw2G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	509, 1032
AasI	GACNNNNNNGTC	1 cut(s)	599
Acc65I	GGTACC	1 cut(s)	878
AccB1I	GGYRCC	3 cut(s)	236, 398, 878
AccBSI	CCGCTC	2 cut(s)	731, 1198
AccII	CGCG	1 cut(s)	1390
AciI	CCGC	6 cut(s)	288, 325, 521, 729, 1198, 1283
AclI	AACGTT	1 cut(s)	44
AclWI	GGATC	5 cut(s)	474, 518, 531, 617, 677
AcoI	YGGCCR	2 cut(s)	3, 1054
AcsI	RAATTY	1 cut(s)	652
AcyI	GRCGYC	1 cut(s)	237
AfaI	GTAC	4 cut(s)	880, 1105, 1251, 1393
AfiI	CCNNNNNNNGG	1 cut(s)	233
AgsI	TTSAA	1 cut(s)	361
AjnI	CCWGG	3 cut(s)	625, 985, 1099
Alw21I	GWGCWC	2 cut(s)	922, 1320
Alw26I	GTCTC	1 cut(s)	1320
AlwI	GGATC	5 cut(s)	474, 518, 531, 617, 677
AlwNI	CAGNNNCTG	2 cut(s)	87, 785
AoxI	GGCC	3 cut(s)	3, 1054, 1275
ApeKI	GCWGC	4 cut(s)	81, 430, 752, 1223
ApoI	RAATTY	1 cut(s)	652
AseI	ATTAAT	1 cut(s)	297
Asp718I	GGTACC	1 cut(s)	878
AspLEI	GCGC	4 cut(s)	239, 417, 744, 1390
AspS9I	GGNCC	1 cut(s)	1275
AsuC2I	CCSGG	1 cut(s)	234
AsuHPI	GGTGA	6 cut(s)	157, 373, 568, 595, 625, 863
BalI	TGGCCA	2 cut(s)	5, 1056
BamHI	GGATCC	1 cut(s)	523
BanI	GGYRCC	3 cut(s)	236, 398, 878
BanII	GRGCYC	1 cut(s)	1320
BbsI	GAAGAC	1 cut(s)	60
Bbv12I	GWGCWC	2 cut(s)	922, 1320
BbvI	GCAGC	4 cut(s)	93, 442, 764, 1210
BccI	CCATC	2 cut(s)	39, 1333
BceAI	ACGGC	1 cut(s)	226
BcgI	CGANNNNNNTGC	2 cut(s)	1148, 1182
BciT130I	CCWGG	3 cut(s)	627, 987, 1101
BcnI	CCSGG	1 cut(s)	234
BcoDI	GTCTC	1 cut(s)	1320
BfaI	CTAG	2 cut(s)	180, 1434
BfmI	CTRYAG	1 cut(s)	1349
BfoI	RGCGCY	2 cut(s)	240, 745
BglII	AGATCT	2 cut(s)	766, 1171
BisI	GCNGC	4 cut(s)	82, 431, 753, 1224
BlpI	GCTNAGC	1 cut(s)	1194
BlsI	GCNGC	4 cut(s)	83, 432, 754, 1225
Bme1390I	CCNGG	4 cut(s)	234, 627, 987, 1101
BmgT120I	GGNCC	1 cut(s)	1275
BmiI	GGNNCC	5 cut(s)	238, 400, 525, 870, 880
BmrFI	CCNGG	4 cut(s)	234, 627, 987, 1101
BmrI	ACTGGG	1 cut(s)	922
BmsI	GCATC	2 cut(s)	195, 781
BmuI	ACTGGG	1 cut(s)	922
BpiI	GAAGAC	1 cut(s)	60
BplI	GAGNNNNNCTC	2 cut(s)	455, 487
Bpu1102I	GCTNAGC	1 cut(s)	1194
BpuEI	CTTGAG	3 cut(s)	468, 791, 1334
BpuMI	CCSGG	1 cut(s)	234
Bsa29I	ATCGAT	2 cut(s)	146, 1115
BsaBI	GATNNNNATC	1 cut(s)	858
BsaHI	GRCGYC	1 cut(s)	237
BsaI	GGTCTC	1 cut(s)	1320
BsaJI	CCNNGG	7 cut(s)	6, 232, 240, 626, 986, 1278, 1444
BsaWI	WCCGGW	1 cut(s)	689
Bsc4I	CCNNNNNNNGG	1 cut(s)	233
Bse1I	ACTGG	2 cut(s)	917, 1399
Bse8I	GATNNNNATC	1 cut(s)	858
BseBI	CCWGG	3 cut(s)	627, 987, 1101
BseCI	ATCGAT	2 cut(s)	146, 1115
BseDI	CCNNGG	7 cut(s)	6, 232, 240, 626, 986, 1278, 1444
BseGI	GGATG	2 cut(s)	31, 186
BseJI	GATNNNNATC	1 cut(s)	858
BseLI	CCNNNNNNNGG	1 cut(s)	233
BseMII	CTCAG	4 cut(s)	531, 603, 612, 1185
BseNI	ACTGG	2 cut(s)	917, 1399
BseRI	GAGGAG	4 cut(s)	28, 213, 265, 721
BseXI	GCAGC	4 cut(s)	93, 442, 764, 1210
Bsh1236I	CGCG	1 cut(s)	1390
Bsh1285I	CGRYCG	1 cut(s)	953
BshFI	GGCC	3 cut(s)	5, 1056, 1277
BshNI	GGYRCC	3 cut(s)	236, 398, 878
BshVI	ATCGAT	2 cut(s)	146, 1115
BsiEI	CGRYCG	1 cut(s)	953
BsiHKAI	GWGCWC	2 cut(s)	922, 1320
BsiSI	CCGG	3 cut(s)	78, 234, 690
BslFI	GGGAC	1 cut(s)	293
BslI	CCNNNNNNNGG	1 cut(s)	233
BsmAI	GTCTC	1 cut(s)	1320
BsmFI	GGGAC	1 cut(s)	293
BsnI	GGCC	3 cut(s)	5, 1056, 1277
Bso31I	GGTCTC	1 cut(s)	1320
Bsp1286I	GDGCHC	2 cut(s)	922, 1320
Bsp143I	GATC	9 cut(s)	143, 466, 523, 543, 622, 669, 766, 950, 1171
Bsp1720I	GCTNAGC	1 cut(s)	1194
Bsp19I	CCATGG	1 cut(s)	6
BspACI	CCGC	6 cut(s)	288, 325, 521, 729, 1198, 1283
BspANI	GGCC	3 cut(s)	5, 1056, 1277
BspCNI	CTCAG	4 cut(s)	532, 602, 611, 1186
BspDI	ATCGAT	2 cut(s)	146, 1115
BspFNI	CGCG	1 cut(s)	1390
BspLI	GGNNCC	5 cut(s)	238, 400, 525, 870, 880
BspMAI	CTGCAG	1 cut(s)	1353
BspPI	GGATC	5 cut(s)	474, 518, 531, 617, 677
BspT107I	GGYRCC	3 cut(s)	236, 398, 878
BspTNI	GGTCTC	1 cut(s)	1320
BsrBI	CCGCTC	2 cut(s)	731, 1198
BsrI	ACTGG	2 cut(s)	917, 1399
BssECI	CCNNGG	7 cut(s)	6, 232, 240, 626, 986, 1278, 1444
BssMI	GATC	9 cut(s)	143, 466, 523, 543, 622, 669, 766, 950, 1171
BssNI	GRCGYC	1 cut(s)	237
BssT1I	CCWWGG	3 cut(s)	6, 240, 1278
Bst2UI	CCWGG	3 cut(s)	627, 987, 1101
Bst4CI	ACNGT	5 cut(s)	781, 787, 883, 889, 1093
Bst6I	CTCTTC	2 cut(s)	79, 383
BstACI	GRCGYC	1 cut(s)	237
BstAPI	GCANNNNNTGC	1 cut(s)	87
BstC8I	GCNNGC	1 cut(s)	258
BstDEI	CTNAG	4 cut(s)	540, 589, 598, 1194
BstDSI	CCRYGG	2 cut(s)	6, 1444
BstF5I	GGATG	2 cut(s)	31, 186
BstFNI	CGCG	1 cut(s)	1390
BstH2I	RGCGCY	2 cut(s)	240, 745
BstHHI	GCGC	4 cut(s)	239, 417, 744, 1390
BstKTI	GATC	9 cut(s)	146, 469, 526, 546, 625, 672, 769, 953, 1174
BstMAI	GTCTC	1 cut(s)	1320
BstMBI	GATC	9 cut(s)	143, 466, 523, 543, 622, 669, 766, 950, 1171
BstMCI	CGRYCG	1 cut(s)	953
BstMWI	GCNNNNNNNGC	4 cut(s)	87, 439, 1144, 1229
BstNI	CCWGG	3 cut(s)	627, 987, 1101
BstNSI	RCATGY	1 cut(s)	260
BstSCI	CCNGG	4 cut(s)	232, 625, 985, 1099
BstSFI	CTRYAG	1 cut(s)	1349
BstUI	CGCG	1 cut(s)	1390
BstV1I	GCAGC	4 cut(s)	93, 442, 764, 1210
BstV2I	GAAGAC	1 cut(s)	60
BstX2I	RGATCY	5 cut(s)	466, 523, 622, 766, 1171
BstYI	RGATCY	5 cut(s)	466, 523, 622, 766, 1171
Bsu15I	ATCGAT	2 cut(s)	146, 1115
BsuRI	GGCC	3 cut(s)	5, 1056, 1277
BsuTUI	ATCGAT	2 cut(s)	146, 1115
BtgI	CCRYGG	2 cut(s)	6, 1444
BtsCI	GGATG	2 cut(s)	31, 186
BtsI	GCAGTG	1 cut(s)	1346
BtsIMutI	CAGTG	3 cut(s)	273, 786, 1346
Cac8I	GCNNGC	1 cut(s)	258
CaiI	CAGNNNCTG	2 cut(s)	87, 785
CfoI	GCGC	4 cut(s)	239, 417, 744, 1390
Cfr13I	GGNCC	1 cut(s)	1275
ClaI	ATCGAT	2 cut(s)	146, 1115
Csp6I	GTAC	4 cut(s)	879, 1104, 1250, 1392
CspCI	CAANNNNNGTGG	2 cut(s)	561, 596
CviAII	CATG	4 cut(s)	7, 257, 1058, 1160
CviQI	GTAC	4 cut(s)	879, 1104, 1250, 1392
DdeI	CTNAG	4 cut(s)	540, 589, 598, 1194
DinI	GGCGCC	1 cut(s)	238
DpnI	GATC	9 cut(s)	145, 468, 525, 545, 624, 671, 768, 952, 1173
DpnII	GATC	9 cut(s)	143, 466, 523, 543, 622, 669, 766, 950, 1171
DrdI	GACNNNNNNGTC	1 cut(s)	599
DseDI	GACNNNNNNGTC	1 cut(s)	599
EaeI	YGGCCR	2 cut(s)	3, 1054
Eam1104I	CTCTTC	2 cut(s)	79, 383
EarI	CTCTTC	2 cut(s)	79, 383
Ecl136II	GAGCTC	1 cut(s)	1318
Eco130I	CCWWGG	3 cut(s)	6, 240, 1278
Eco24I	GRGCYC	1 cut(s)	1320
Eco31I	GGTCTC	1 cut(s)	1320
Eco32I	GATATC	2 cut(s)	586, 1439
Eco53kI	GAGCTC	1 cut(s)	1318
EcoICRI	GAGCTC	1 cut(s)	1318
EcoRII	CCWGG	3 cut(s)	625, 985, 1099
EcoRV	GATATC	2 cut(s)	586, 1439
EcoT14I	CCWWGG	3 cut(s)	6, 240, 1278
EcoT38I	GRGCYC	1 cut(s)	1320
EgeI	GGCGCC	1 cut(s)	238
EheI	GGCGCC	1 cut(s)	238
ErhI	CCWWGG	3 cut(s)	6, 240, 1278
FaeI	CATG	4 cut(s)	10, 260, 1061, 1163
FaqI	GGGAC	1 cut(s)	293
FatI	CATG	4 cut(s)	6, 256, 1057, 1159
FauI	CCCGC	2 cut(s)	318, 1191
Fnu4HI	GCNGC	4 cut(s)	82, 431, 753, 1224
FokI	GGATG	2 cut(s)	18, 173
FriOI	GRGCYC	1 cut(s)	1320
Fsp4HI	GCNGC	4 cut(s)	82, 431, 753, 1224
FspBI	CTAG	2 cut(s)	180, 1434
GlaI	GCGC	4 cut(s)	238, 416, 743, 1389
GluI	GCNGC	4 cut(s)	82, 431, 753, 1224
HaeII	RGCGCY	2 cut(s)	240, 745
HaeIII	GGCC	3 cut(s)	5, 1056, 1277
HapII	CCGG	3 cut(s)	78, 234, 690
HhaI	GCGC	4 cut(s)	239, 417, 744, 1390
Hin1I	GRCGYC	1 cut(s)	237
Hin1II	CATG	4 cut(s)	10, 260, 1061, 1163
Hin6I	GCGC	4 cut(s)	237, 415, 742, 1388
HinP1I	GCGC	4 cut(s)	237, 415, 742, 1388
HincII	GTYRAC	1 cut(s)	823
HindII	GTYRAC	1 cut(s)	823
HindIII	AAGCTT	1 cut(s)	1145
HinfI	GANTC	5 cut(s)	341, 830, 1112, 1418, 1430
HpaI	GTTAAC	1 cut(s)	823
HpaII	CCGG	3 cut(s)	78, 234, 690
HphI	GGTGA	6 cut(s)	157, 373, 568, 595, 625, 863
Hpy166II	GTNNAC	3 cut(s)	497, 823, 944
Hpy188I	TCNGA	9 cut(s)	103, 303, 528, 592, 674, 835, 1041, 1242, 1297
Hpy188III	TCNNGA	5 cut(s)	141, 764, 770, 1169, 1434
Hpy8I	GTNNAC	3 cut(s)	497, 823, 944
HpyAV	CCTTC	5 cut(s)	124, 129, 805, 882, 1017
HpyCH4III	ACNGT	5 cut(s)	781, 787, 883, 889, 1093
HpyCH4IV	ACGT	3 cut(s)	44, 493, 1164
HpyCH4V	TGCA	8 cut(s)	320, 646, 800, 1061, 1132, 1159, 1346, 1351
HpyF10VI	GCNNNNNNNGC	4 cut(s)	87, 439, 1144, 1229
HpyF3I	CTNAG	4 cut(s)	540, 589, 598, 1194
HpySE526I	ACGT	3 cut(s)	44, 493, 1164
Hsp92I	GRCGYC	1 cut(s)	237
Hsp92II	CATG	4 cut(s)	10, 260, 1061, 1163
HspAI	GCGC	4 cut(s)	237, 415, 742, 1388
KasI	GGCGCC	1 cut(s)	236
KpnI	GGTACC	1 cut(s)	882
KspAI	GTTAAC	1 cut(s)	823
Kzo9I	GATC	9 cut(s)	143, 466, 523, 543, 622, 669, 766, 950, 1171
LmnI	GCTCC	3 cut(s)	28, 736, 868
Lsp1109I	GCAGC	4 cut(s)	93, 442, 764, 1210
LweI	GCATC	2 cut(s)	195, 781
MaeI	CTAG	2 cut(s)	180, 1434
MaeII	ACGT	3 cut(s)	44, 493, 1164
MaeIII	GTNAC	7 cut(s)	152, 329, 530, 601, 613, 781, 883
MalI	GATC	9 cut(s)	145, 468, 525, 545, 624, 671, 768, 952, 1173
MbiI	CCGCTC	2 cut(s)	731, 1198
MboI	GATC	9 cut(s)	143, 466, 523, 543, 622, 669, 766, 950, 1171
MboII	GAAGA	5 cut(s)	60, 66, 370, 834, 1410
MflI	RGATCY	5 cut(s)	466, 523, 622, 766, 1171
MhlI	GDGCHC	2 cut(s)	922, 1320
MlsI	TGGCCA	2 cut(s)	5, 1056
MluCI	AATT	6 cut(s)	576, 652, 678, 1202, 1218, 1401
MluNI	TGGCCA	2 cut(s)	5, 1056
Mly113I	GGCGCC	1 cut(s)	237
MlyI	GAGTC	1 cut(s)	1427
MmeI	TCCRAC	1 cut(s)	251
Mox20I	TGGCCA	2 cut(s)	5, 1056
MscI	TGGCCA	2 cut(s)	5, 1056
MseI	TTAA	6 cut(s)	297, 435, 656, 822, 903, 924
MslI	CAYNNNNRTG	1 cut(s)	611
Msp20I	TGGCCA	2 cut(s)	5, 1056
MspA1I	CMGCKG	1 cut(s)	64
MspI	CCGG	3 cut(s)	78, 234, 690
MspR9I	CCNGG	4 cut(s)	234, 627, 987, 1101
MvaI	CCWGG	3 cut(s)	627, 987, 1101
MvnI	CGCG	1 cut(s)	1390
MwoI	GCNNNNNNNGC	4 cut(s)	87, 439, 1144, 1229
NarI	GGCGCC	1 cut(s)	237
NciI	CCSGG	1 cut(s)	234
NcoI	CCATGG	1 cut(s)	6
NdeII	GATC	9 cut(s)	143, 466, 523, 543, 622, 669, 766, 950, 1171
NlaIII	CATG	4 cut(s)	10, 260, 1061, 1163
NlaIV	GGNNCC	5 cut(s)	238, 400, 525, 870, 880
NmuCI	GTSAC	4 cut(s)	152, 601, 613, 781
NspI	RCATGY	1 cut(s)	260
PaeI	GCATGC	1 cut(s)	260
PcsI	WCGNNNNNNNCGW	1 cut(s)	279
PfeI	GAWTC	4 cut(s)	341, 830, 1112, 1430
PkrI	GCNGC	4 cut(s)	83, 432, 754, 1225
Ple19I	CGATCG	1 cut(s)	953
PleI	GAGTC	1 cut(s)	1426
PluTI	GGCGCC	1 cut(s)	240
PpsI	GAGTC	1 cut(s)	1426
PshBI	ATTAAT	1 cut(s)	297
PsiI	TTATAA	2 cut(s)	509, 1032
Psp124BI	GAGCTC	1 cut(s)	1320
Psp1406I	AACGTT	1 cut(s)	44
Psp6I	CCWGG	3 cut(s)	625, 985, 1099
PspGI	CCWGG	3 cut(s)	625, 985, 1099
PspN4I	GGNNCC	5 cut(s)	238, 400, 525, 870, 880
PspPI	GGNCC	1 cut(s)	1275
PsrI	GAACNNNNNNTAC	2 cut(s)	966, 998
PstI	CTGCAG	1 cut(s)	1353
PstNI	CAGNNNCTG	2 cut(s)	87, 785
PsuI	RGATCY	5 cut(s)	466, 523, 622, 766, 1171
PvuI	CGATCG	1 cut(s)	953
PvuII	CAGCTG	1 cut(s)	64
RsaI	GTAC	4 cut(s)	880, 1105, 1251, 1393
RsaNI	GTAC	4 cut(s)	879, 1104, 1250, 1392
RseI	CAYNNNNRTG	1 cut(s)	611
SacI	GAGCTC	1 cut(s)	1320
SaqAI	TTAA	6 cut(s)	297, 435, 656, 822, 903, 924
SatI	GCNGC	4 cut(s)	82, 431, 753, 1224
Sau3AI	GATC	9 cut(s)	143, 466, 523, 543, 622, 669, 766, 950, 1171
Sau96I	GGNCC	1 cut(s)	1275
SchI	GAGTC	1 cut(s)	1427
ScrFI	CCNGG	4 cut(s)	234, 627, 987, 1101
SduI	GDGCHC	2 cut(s)	922, 1320
SfaNI	GCATC	2 cut(s)	195, 781
SfcI	CTRYAG	1 cut(s)	1349
SfoI	GGCGCC	1 cut(s)	238
SmiMI	CAYNNNNRTG	1 cut(s)	611
SmlI	CTYRAG	3 cut(s)	483, 770, 1313
SmoI	CTYRAG	3 cut(s)	483, 770, 1313
SphI	GCATGC	1 cut(s)	260
Sse9I	AATT	6 cut(s)	576, 652, 678, 1202, 1218, 1401
SsiI	CCGC	6 cut(s)	288, 325, 521, 729, 1198, 1283
SspDI	GGCGCC	1 cut(s)	236
SspMI	CTAG	2 cut(s)	180, 1434
SstI	GAGCTC	1 cut(s)	1320
StyD4I	CCNGG	4 cut(s)	232, 625, 985, 1099
StyI	CCWWGG	3 cut(s)	6, 240, 1278
TaaI	ACNGT	5 cut(s)	781, 787, 883, 889, 1093
TaiI	ACGT	3 cut(s)	47, 496, 1167
TaqI	TCGA	8 cut(s)	140, 146, 339, 949, 1021, 1115, 1152, 1168
TasI	AATT	6 cut(s)	576, 652, 678, 1202, 1218, 1401
TatI	WGTACW	1 cut(s)	1249
TfiI	GAWTC	4 cut(s)	341, 830, 1112, 1430
Tru1I	TTAA	6 cut(s)	297, 435, 656, 822, 903, 924
Tru9I	TTAA	6 cut(s)	297, 435, 656, 822, 903, 924
TscAI	CASTG	3 cut(s)	273, 786, 1353
TseFI	GTSAC	4 cut(s)	152, 601, 613, 781
TseI	GCWGC	4 cut(s)	81, 430, 752, 1223
Tsp45I	GTSAC	4 cut(s)	152, 601, 613, 781
TspDTI	ATGAA	3 cut(s)	111, 747, 1125
TspGWI	ACGGA	1 cut(s)	1433
TspRI	CASTG	3 cut(s)	273, 786, 1353
VspI	ATTAAT	1 cut(s)	297
XapI	RAATTY	1 cut(s)	652
XbaI	TCTAGA	1 cut(s)	1433
XceI	RCATGY	1 cut(s)	260
XspI	CTAG	2 cut(s)	180, 1434

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.