RchiOBHm_Chr2g0092401

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016233
Physical Location & Seq
Forward (+)
5921297 .. 5922157
861 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
ATGAGACCAGGATCGGGTGATGCTACCCAACCGGCCAAGCCCCGGTGGGCAGATATCGATGAGGAGGACGGACAGGAGGAGTTGGACCTCCTCCTTTTGCCGCCAAAGCAGGTGATTGGCCCCGACGAGAAGGGGATGGAGAAGGTGATTGAATACAAATTCAATGAAAAGGGTCAGAAGGTCAAGATCACCACCACCACCACCACCACCACCAGGACCAAATCTCACCTCATGAGTAAGGCGGCCGTGGAGAGGCGCTCCTGGCCAAAATTTGGTGGTGCCGTCAATGAAGACGCCGGTGCCAATCTGACAGCGGTTTCCAAAGAGGAGATCATGCTTGAACGCCCCAAGGCTCTTGGCGCCAACAAGGCACAAGAACTACCAAAGAGTACTAGTGGTAATGGAGGAGGCAATACTACTCTCAAGGTTTGTAGGACTTGTGGTAAGAAAGGTGACCACTGGACTGCACAGTGCCCATACAAAGATCTTGCCCCGCCGAGTTCCGAAGATATTAGCTCATCCGAGGCAGCCACAACTAGCAAGATTGGTACATACGTTCCTCCGGGTTTGAGAGAAGGAGTGAAGAGGGCTCTGAGGAATGATGAAAATTCAGTTCGGGTGAGCAACCTTTCGGAGGATACCCGGGACCCCGATTTGCATGAACTTTTCAGTCCTTTTGGTGCCATAAGCCGTGCTCATGTTGTTCTTGAGAGAGACACCCAAGTTAGCAGAGGATTTGGTTTTGTTGACTTTCTCAACAAGGAACACGGCCAAAGAGCCATAGACAAACTCAATGGGTATGGTTATGACAATCTCATCCTTCGGGTAGAATGGGCCACGCCCCGGCCCAGGCCCAATTGA

Protein Analysis

286

Amino Acids

31.44

Weight (kDa)

7.69

Isoelectric Point (pI)

36.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3g PF12353 37 - 163 1.5e-29 Eukaryotic translation initiation factor 3 subunit G
RRM_1 PF00076 205 - 271 1.4e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06650
rosa_chinensis RchiOBHm_Chr2g0092401 RchiOBHm_Chr6g0294311
rosa_laevigata RLG00000016307
rosa_multiflora Rmu_co8481273.1_g000001 Rmu_sc0001195.1_g000020
rosa_roxburghii Rroxscaffold_2G00149150
rosa_rugosa Rorug02G0025800
rosa_samantha Rh2AG071800 Rh2BG071800 Rh2CG073200 Rh2DG070300 Rh6CG369900
rosa_wichuraiana Rw2G006080 Rw6G031010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 100
AccB1I GGYRCC 4 cut(s) 278, 299, 359, 680
AccB7I CCANNNNNTGG 1 cut(s) 272
AciI CCGC 4 cut(s) 101, 242, 314, 494
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 4 cut(s) 33, 243, 263, 769
AcsI RAATTY 3 cut(s) 158, 269, 607
AcyI GRCGYC 2 cut(s) 294, 360
AfaI GTAC 2 cut(s) 391, 550
AfiI CCNNNNNNNGG 8 cut(s) 14, 42, 213, 252, 272, 634, 843, 849
AgsI TTSAA 3 cut(s) 152, 163, 341
AhlI ACTAGT 1 cut(s) 392
AjnI CCWGG 4 cut(s) 7, 212, 260, 848
AluBI AGCT 1 cut(s) 516
AluI AGCT 1 cut(s) 516
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 1 cut(s) 708
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 642
AoxI GGCC 8 cut(s) 33, 118, 243, 263, 769, 834, 845, 851
ApeKI GCWGC 1 cut(s) 527
ApoI RAATTY 3 cut(s) 158, 269, 607
AspLEI GCGC 2 cut(s) 258, 362
AspS9I GGNCC 7 cut(s) 85, 119, 216, 646, 834, 846, 852
AsuC2I CCSGG 5 cut(s) 43, 564, 643, 644, 844
AsuHPI GGTGA 7 cut(s) 29, 124, 157, 181, 218, 464, 631
AvaI CYCGRG 1 cut(s) 642
AvaII GGWCC 3 cut(s) 85, 216, 646
BaeGI GKGCMC 1 cut(s) 476
BaeI ACNNNNGTAYC 2 cut(s) 540, 573
BalI TGGCCA 1 cut(s) 265
BanI GGYRCC 4 cut(s) 278, 299, 359, 680
BanII GRGCYC 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 697
BbvI GCAGC 1 cut(s) 539
BccI CCATC 1 cut(s) 130
BceAI ACGGC 4 cut(s) 230, 266, 675, 784
BciT130I CCWGG 4 cut(s) 9, 214, 262, 850
BciVI GTATCC 1 cut(s) 631
BcnI CCSGG 5 cut(s) 43, 564, 643, 644, 844
BcoDI GTCTC 1 cut(s) 708
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 2 cut(s) 393, 537
BfoI RGCGCY 2 cut(s) 259, 363
BfuAI ACCTGC 1 cut(s) 100
BfuI GTATCC 1 cut(s) 631
BglI GCCNNNNNGGC 1 cut(s) 368
BglII AGATCT 1 cut(s) 484
BisI GCNGC 3 cut(s) 101, 243, 528
BlsI GCNGC 3 cut(s) 102, 244, 529
BmcAI AGTACT 1 cut(s) 391
Bme1390I CCNGG 9 cut(s) 9, 43, 214, 262, 564, 643, 644, 844, 850
Bme18I GGWCC 3 cut(s) 85, 216, 646
BmeT110I CYCGRG 1 cut(s) 642
BmgT120I GGNCC 7 cut(s) 85, 119, 216, 646, 834, 846, 852
BmiI GGNNCC 7 cut(s) 121, 280, 301, 361, 647, 648, 682
BmrFI CCNGG 9 cut(s) 9, 43, 214, 262, 564, 643, 644, 844, 850
BmsI GCATC 1 cut(s) 10
BpiI GAAGAC 1 cut(s) 297
BplI GAGNNNNNCTC 2 cut(s) 242, 274
BpuEI CTTGAG 2 cut(s) 407, 728
BpuMI CCSGG 5 cut(s) 43, 564, 643, 644, 844
Bsa29I ATCGAT 1 cut(s) 57
BsaHI GRCGYC 2 cut(s) 294, 360
BsaJI CCNNGG 7 cut(s) 41, 246, 348, 522, 642, 842, 848
Bsc4I CCNNNNNNNGG 8 cut(s) 14, 42, 213, 252, 272, 634, 843, 849
Bse118I RCCGGY 2 cut(s) 31, 296
Bse1I ACTGG 1 cut(s) 464
BseBI CCWGG 4 cut(s) 9, 214, 262, 850
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 7 cut(s) 41, 246, 348, 522, 642, 842, 848
BseGI GGATG 3 cut(s) 141, 518, 816
BseLI CCNNNNNNNGG 8 cut(s) 14, 42, 213, 252, 272, 634, 843, 849
BseMII CTCAG 1 cut(s) 584
BseNI ACTGG 1 cut(s) 464
BseRI GAGGAG 5 cut(s) 77, 80, 92, 341, 420
BseSI GKGCMC 1 cut(s) 476
BseX3I CGGCCG 1 cut(s) 243
BseXI GCAGC 1 cut(s) 539
BsgI GTGCAG 1 cut(s) 450
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 8 cut(s) 35, 120, 245, 265, 771, 836, 847, 853
BshNI GGYRCC 4 cut(s) 278, 299, 359, 680
BshVI ATCGAT 1 cut(s) 57
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 697
BsiHKCI CYCGRG 1 cut(s) 642
BsiSI CCGG 6 cut(s) 32, 43, 297, 563, 643, 844
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 8 cut(s) 14, 42, 213, 252, 272, 634, 843, 849
BsmAI GTCTC 1 cut(s) 708
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 8 cut(s) 35, 120, 245, 265, 771, 836, 847, 853
BsoBI CYCGRG 1 cut(s) 642
Bsp1286I GDGCHC 3 cut(s) 476, 592, 697
Bsp143I GATC 4 cut(s) 11, 186, 330, 484
BspACI CCGC 4 cut(s) 101, 242, 314, 494
BspANI GGCC 8 cut(s) 35, 120, 245, 265, 771, 836, 847, 853
BspCNI CTCAG 1 cut(s) 585
BspDI ATCGAT 1 cut(s) 57
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 7 cut(s) 121, 280, 301, 361, 647, 648, 682
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 4 cut(s) 278, 299, 359, 680
BsrFI RCCGGY 2 cut(s) 31, 296
BsrI ACTGG 1 cut(s) 464
BssAI RCCGGY 2 cut(s) 31, 296
BssECI CCNNGG 7 cut(s) 41, 246, 348, 522, 642, 842, 848
BssMI GATC 4 cut(s) 11, 186, 330, 484
BssNI GRCGYC 2 cut(s) 294, 360
BssT1I CCWWGG 1 cut(s) 348
Bst2UI CCWGG 4 cut(s) 9, 214, 262, 850
Bst4CI ACNGT 1 cut(s) 471
Bst6I CTCTTC 1 cut(s) 578
BstACI GRCGYC 2 cut(s) 294, 360
BstDEI CTNAG 1 cut(s) 593
BstDSI CCRYGG 1 cut(s) 246
BstEII GGTNACC 1 cut(s) 452
BstENI CCTNNNNNAGG 1 cut(s) 632
BstF5I GGATG 3 cut(s) 141, 518, 816
BstH2I RGCGCY 2 cut(s) 259, 363
BstHHI GCGC 2 cut(s) 258, 362
BstKTI GATC 4 cut(s) 14, 189, 333, 487
BstMAI GTCTC 1 cut(s) 708
BstMBI GATC 4 cut(s) 11, 186, 330, 484
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 4 cut(s) 106, 262, 359, 368
BstNI CCWGG 4 cut(s) 9, 214, 262, 850
BstPI GGTNACC 1 cut(s) 452
BstSCI CCNGG 9 cut(s) 7, 41, 212, 260, 562, 641, 642, 842, 848
BstSLI GKGCMC 1 cut(s) 476
BstV1I GCAGC 1 cut(s) 539
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
BstZI CGGCCG 1 cut(s) 243
Bsu15I ATCGAT 1 cut(s) 57
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 8 cut(s) 35, 120, 245, 265, 771, 836, 847, 853
BsuTUI ATCGAT 1 cut(s) 57
BtgI CCRYGG 1 cut(s) 246
BtsCI GGATG 3 cut(s) 141, 518, 816
BtsIMutI CAGTG 2 cut(s) 457, 476
BveI ACCTGC 1 cut(s) 100
CciI TCATGA 1 cut(s) 231
CfoI GCGC 2 cut(s) 258, 362
Cfr10I RCCGGY 2 cut(s) 31, 296
Cfr13I GGNCC 7 cut(s) 85, 119, 216, 646, 834, 846, 852
Cfr9I CCCGGG 1 cut(s) 642
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 1 cut(s) 302
Csp6I GTAC 2 cut(s) 390, 549
CviAII CATG 4 cut(s) 232, 334, 659, 698
CviQI GTAC 2 cut(s) 390, 549
DdeI CTNAG 1 cut(s) 593
DinI GGCGCC 1 cut(s) 361
DpnI GATC 4 cut(s) 13, 188, 332, 486
DpnII GATC 4 cut(s) 11, 186, 330, 484
EaeI YGGCCR 4 cut(s) 33, 243, 263, 769
EagI CGGCCG 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 578
EarI CTCTTC 1 cut(s) 578
EclXI CGGCCG 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 348
Eco24I GRGCYC 1 cut(s) 592
Eco32I GATATC 1 cut(s) 55
Eco47I GGWCC 3 cut(s) 85, 216, 646
Eco52I CGGCCG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 642
Eco91I GGTNACC 1 cut(s) 452
EcoNI CCTNNNNNAGG 1 cut(s) 632
EcoO109I RGGNCCY 1 cut(s) 646
EcoO65I GGTNACC 1 cut(s) 452
EcoRII CCWGG 4 cut(s) 7, 212, 260, 848
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 348
EcoT38I GRGCYC 1 cut(s) 592
EgeI GGCGCC 1 cut(s) 361
EheI GGCGCC 1 cut(s) 361
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 4 cut(s) 235, 337, 662, 701
FaqI GGGAC 1 cut(s) 659
FatI CATG 4 cut(s) 231, 333, 658, 697
FauI CCCGC 1 cut(s) 501
Fnu4HI GCNGC 3 cut(s) 101, 243, 528
FokI GGATG 3 cut(s) 148, 505, 803
FriOI GRGCYC 1 cut(s) 592
Fsp4HI GCNGC 3 cut(s) 101, 243, 528
FspBI CTAG 2 cut(s) 393, 537
GlaI GCGC 2 cut(s) 257, 361
GluI GCNGC 3 cut(s) 101, 243, 528
HaeII RGCGCY 2 cut(s) 259, 363
HaeIII GGCC 8 cut(s) 35, 120, 245, 265, 771, 836, 847, 853
HapII CCGG 6 cut(s) 32, 43, 297, 563, 643, 844
HgaI GACGC 1 cut(s) 302
HhaI GCGC 2 cut(s) 258, 362
Hin1I GRCGYC 2 cut(s) 294, 360
Hin1II CATG 4 cut(s) 235, 337, 662, 701
Hin6I GCGC 2 cut(s) 256, 360
HinP1I GCGC 2 cut(s) 256, 360
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HpaII CCGG 6 cut(s) 32, 43, 297, 563, 643, 844
HphI GGTGA 7 cut(s) 29, 124, 157, 181, 218, 464, 631
Hpy166II GTNNAC 1 cut(s) 748
Hpy188I TCNGA 6 cut(s) 177, 309, 505, 523, 594, 634
Hpy188III TCNNGA 3 cut(s) 184, 232, 707
Hpy8I GTNNAC 1 cut(s) 748
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 5 cut(s) 124, 136, 172, 569, 830
HpyCH4III ACNGT 1 cut(s) 471
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 2 cut(s) 467, 658
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 262, 359, 368
HpyF3I CTNAG 1 cut(s) 593
HpySE526I ACGT 1 cut(s) 555
Hsp92I GRCGYC 2 cut(s) 294, 360
Hsp92II CATG 4 cut(s) 235, 337, 662, 701
HspAI GCGC 2 cut(s) 256, 360
KasI GGCGCC 1 cut(s) 359
KflI GGGWCCC 1 cut(s) 646
Kzo9I GATC 4 cut(s) 11, 186, 330, 484
LmnI GCTCC 1 cut(s) 263
Lsp1109I GCAGC 1 cut(s) 539
LweI GCATC 1 cut(s) 10
MaeI CTAG 2 cut(s) 393, 537
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 1 cut(s) 452
MalI GATC 4 cut(s) 13, 188, 332, 486
MboI GATC 4 cut(s) 11, 186, 330, 484
MboII GAAGA 3 cut(s) 302, 518, 595
MfeI CAATTG 1 cut(s) 856
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 3 cut(s) 476, 592, 697
MlsI TGGCCA 1 cut(s) 265
MluCI AATT 4 cut(s) 158, 269, 607, 856
MluNI TGGCCA 1 cut(s) 265
Mly113I GGCGCC 1 cut(s) 360
MmeI TCCRAC 1 cut(s) 63
Mox20I TGGCCA 1 cut(s) 265
MscI TGGCCA 1 cut(s) 265
Msp20I TGGCCA 1 cut(s) 265
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 6 cut(s) 32, 43, 297, 563, 643, 844
MspR9I CCNGG 9 cut(s) 9, 43, 214, 262, 564, 643, 644, 844, 850
MunI CAATTG 1 cut(s) 856
MvaI CCWGG 4 cut(s) 9, 214, 262, 850
MwoI GCNNNNNNNGC 4 cut(s) 106, 262, 359, 368
NarI GGCGCC 1 cut(s) 360
NciI CCSGG 5 cut(s) 43, 564, 643, 644, 844
NdeII GATC 4 cut(s) 11, 186, 330, 484
NlaIII CATG 4 cut(s) 235, 337, 662, 701
NlaIV GGNNCC 7 cut(s) 121, 280, 301, 361, 647, 648, 682
NmeAIII GCCGAG 1 cut(s) 522
NmuCI GTSAC 1 cut(s) 452
PagI TCATGA 1 cut(s) 231
PaqCI CACCTGC 1 cut(s) 100
PflMI CCANNNNNTGG 1 cut(s) 272
PkrI GCNGC 3 cut(s) 102, 244, 529
PluTI GGCGCC 1 cut(s) 363
PpuMI RGGWCCY 1 cut(s) 646
Psp5II RGGWCCY 1 cut(s) 646
Psp6I CCWGG 4 cut(s) 7, 212, 260, 848
PspEI GGTNACC 1 cut(s) 452
PspGI CCWGG 4 cut(s) 7, 212, 260, 848
PspN4I GGNNCC 7 cut(s) 121, 280, 301, 361, 647, 648, 682
PspPI GGNCC 7 cut(s) 85, 119, 216, 646, 834, 846, 852
PspPPI RGGWCCY 1 cut(s) 646
PsuI RGATCY 1 cut(s) 484
RsaI GTAC 2 cut(s) 391, 550
RsaNI GTAC 2 cut(s) 390, 549
SatI GCNGC 3 cut(s) 101, 243, 528
Sau3AI GATC 4 cut(s) 11, 186, 330, 484
Sau96I GGNCC 7 cut(s) 85, 119, 216, 646, 834, 846, 852
ScaI AGTACT 1 cut(s) 391
ScrFI CCNGG 9 cut(s) 9, 43, 214, 262, 564, 643, 644, 844, 850
SduI GDGCHC 3 cut(s) 476, 592, 697
SfaNI GCATC 1 cut(s) 10
SfoI GGCGCC 1 cut(s) 361
SgrAI CRCCGGYG 1 cut(s) 296
SinI GGWCC 3 cut(s) 85, 216, 646
SmaI CCCGGG 1 cut(s) 644
SmlI CTYRAG 2 cut(s) 422, 707
SmoI CTYRAG 2 cut(s) 422, 707
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 4 cut(s) 158, 269, 607, 856
SsiI CCGC 4 cut(s) 101, 242, 314, 494
SspDI GGCGCC 1 cut(s) 359
SspMI CTAG 2 cut(s) 393, 537
StyD4I CCNGG 9 cut(s) 7, 41, 212, 260, 562, 641, 642, 842, 848
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 471
TaiI ACGT 1 cut(s) 558
TaqI TCGA 1 cut(s) 57
TasI AATT 4 cut(s) 158, 269, 607, 856
TatI WGTACW 1 cut(s) 389
TauI GCSGC 2 cut(s) 103, 245
TscAI CASTG 2 cut(s) 464, 476
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 1 cut(s) 527
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 4 cut(s) 180, 303, 618, 675
TspGWI ACGGA 1 cut(s) 84
TspMI CCCGGG 1 cut(s) 642
TspRI CASTG 2 cut(s) 464, 476
Van91I CCANNNNNTGG 1 cut(s) 272
VpaK11BI GGWCC 3 cut(s) 85, 216, 646
XagI CCTNNNNNAGG 1 cut(s) 632
XapI RAATTY 3 cut(s) 158, 269, 607
XmaI CCCGGG 1 cut(s) 642
XspI CTAG 2 cut(s) 393, 537
ZrmI AGTACT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.