RchiOBHm_Chr2g0144801

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005772
Physical Location & Seq
Forward (+)
62388299 .. 62397706
9408 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2607 bp
ATGGTCTCTGGGTCGCGAATCGAAGGTGGTGGTGGGACTCAGATAATCTCCGCCGGAGTGAGGAAAACCATCCAATCCATCAAAGAAATAGTGGGCAATCACTCTGATATTGATATCTATTGGGCTCTCAAAGAGACCGATATGGATCCTAATGAAACTGCCCAGAAATTACTCAACCAAGACCCATTTCATGAGGTGAAGAGAAAAAGAGACAAAAAGAAGGACGCGTCACCTAAGAGTATCGGGCAAAAGGTTCCTACCGAGCCAAGAAGACATTTTGAGAGTGCTGGTCAAGGACCAAGATCAAATACAATTTCAGATCGTATTGTGGAGCCTAAAAGACATTTTGAGAGTGCAGGTCAAGGACCAAGACATAATTCATTTTCTGATCGTAATGTTAGAAGAGGAGGTTATGTGCGCAGGGGTTTACCTGGAATCAGCAGAGAGTTTCGTGTTGTGAGAGACAACAGAGCTAACCAAAATATGGATGGAGAAATTAAGCCTGCTTCACCTCAATGTACTACATCTACGAATGAGCAAGTGATTTCAAATGTTTCTGAGAAGGGCCAAACAGGAAATTCAAGCAGTCAAAAGCCATCTAATAGACAGCATTCCTCACAAGCATTGAATGGGCAAACTGATTCTCGAATTAGAACGAACGTTGCTAATTCATCTGGTATGATTAGAAAGGAGACATTAGTGGAGAAGCGAGTTGCTCTTCCAAATTCAGTTTCACGGGTACAGGCAGGGAAACCAAACAACTCCCAACCACATTCTGCATCAAGCACTTCTGTTATTGGGGTGTATTCCTCGTCCACAGATCCTGTTCATGTGCCGTCTCCTGATTCTAGACCATCTGCTTCTGTTGGTGCAATTAAGCGCGAAGTTGGGGTGGTTGGAGTTCGGAAACAGTCTTCTGACAACTCCAAGTCAGCTGTACCAAGTAGTTCTTTCTCTAATACACTTTTGGGAAAAGAGGGCTCTGCAGAATCATTTAGATCTTTCACTGGTATCTCTAAACCTGATCAACTCAGCCAAACTTCTGAATCTGTGATGCCGGTCAGCAGATCTTTCGTAAGTAATCAGCACAGTGTCAGGGCGCATCAACAACCTGTGGGTCATCAGAAAGCTTCCCAGCCTAACAAGGAGTGGAAACCTAAATCAAGTCAGAAGCCAAGCCCTAATAGTCCTGGAGTCATTGGAACACCAACAAAATCTGCTTCTCCTCTTTCTGATGATTCAAAGGTCTCAGAGTCAGAAGCGGCTAAGTTGCAAGATAAGCTTGCCCGAGTAAATATATATGAGAACTGTAATGTTGTGATAGCGCAAAATATTAGAGTCCCTGAGAGTGACCGTTTTCGGCTAACCTTTGGAAGCTTGGGAACAGAGTTTGATTCAACAGGAAACACAGTTAATGGATTTCAAGCTGGAGCTGCGGAGGAGTTGAACAGGGAACCTCCAGCAAGTTTGTCAACATCAGCTCCCGAGTCGCGCAGTGATGAGGCTTCCGGCACGAAGCCAGTAGATTTATTAGATGACCAAGTTAGAAATTCTGGATCTGATTTTTCGGCACCGTCTGCAGTGCCTGAGCATTTGCCTGAGAAAAAAGAGACCTCAAGTCCTCAGAGTTTGGACAATTATGCAGATATTGGTTTGGTTCGAGACAACAGTCCATCCTTTGCACCTTCAGATTCGCAGAACCAAGATCCACCAGAGTTACAAGGTTTTACGGCATTTGATCCTCAGACTGGTTATGATATACCTTATTACAGACCCAGTATGGATGAAAGTGTTTGCGGACAGGGTCTACAATCTCCTCAGGAGGCTTTGAGTTCACATAATGCCAACAGTATTCCTGCATCAACAGTTGCCATGGTACAACAGCAACCACCGCATGTGGCGCAGATGTACCCACAAGTTCATGTTTCACATTATGCTAATCTTATGCCATATCGTCAGTTTATCTCACCAGTATACGTTCCACCAATGGCTGTGCCTGGCTATTCCAATAATCCTGCCTATCCTCACATGTCTAATGGCAACAGCTACTTGCTGATGCCTGGAGGTGGTTCCCACCTAAATGCAAACAGCCTTAAGTATGGAGTTCAGCAATTTAAGCCCGTCCCTGCTGGAAGTCCCACTGGGTTTGGAAATTTTACTAACCCAGCAGGTTATGCAATGAATGCCCCTGGTGTAGTTGGAGGTGCCACTGGACTCGAAGATTCATCAAGAATGAAGTACAAAGATGGCAATCTTTATGTTCCAAATCCACAGGCGGAGACATCAGAGATTTGGATTCAGAACCCAAGGGAGCATCCAGGAATGCAATCCACACCATACTACAACATGCCTGGGCAAACACCTCATGCTCCTTATATGCCGTCCCACGCCAGCCACGCTTCCTTTAATGCAGCTGCAGCACAATCTTCGCACATGCAATTCCCGGGAATGTACCATCCTCCTCAGCCTGCTGCAATGGCCAGTCCGCACCATATGGGCCCTGCTATGCCCGGCAATGTCGGAGTGGGGGTGGCCGCAGCTGCTCCTGGGGCACAGGCGTATCAGCAGCCCCAGTTGAATCATATGAACTGGACAACAAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

868

Amino Acids

93.41

Weight (kDa)

8.81

Isoelectric Point (pI)

57.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 16 - 74 4e-30 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 419
Acc36I ACCTGC 2 cut(s) 347, 2159
AccB1I GGYRCC 2 cut(s) 1570, 2204
AccB7I CCANNNNNTGG 1 cut(s) 484
AccI GTMKAC 2 cut(s) 1807, 1974
AccII CGCG 4 cut(s) 16, 227, 882, 1492
AciI CCGC 8 cut(s) 51, 1262, 1436, 1797, 1892, 2276, 2486, 2535
AclI AACGTT 1 cut(s) 660
AclWI GGATC 6 cut(s) 140, 153, 815, 1564, 1700, 1733
AcoI YGGCCR 2 cut(s) 2478, 2532
AcsI RAATTY 4 cut(s) 577, 724, 1549, 2152
AcuI CTGAAG 1 cut(s) 1671
AfaI GTAC 7 cut(s) 520, 741, 939, 1878, 1910, 2240, 2453
AfiI CCNNNNNNNGG 4 cut(s) 60, 484, 1748, 2066
AflII CTTAAG 1 cut(s) 2093
AflIII ACRYGT 2 cut(s) 225, 2028
AgsI TTSAA 8 cut(s) 549, 582, 628, 1242, 1398, 1424, 1447, 2578
AhdI GACNNNNNGTC 1 cut(s) 1617
AjnI CCWGG 8 cut(s) 430, 1189, 1996, 2059, 2188, 2317, 2350, 2545
AjuI GAANNNNNNNTTGG 6 cut(s) 171, 203, 361, 393, 715, 747
AlwI GGATC 6 cut(s) 140, 153, 815, 1564, 1700, 1733
AlwNI CAGNNNCTG 2 cut(s) 824, 1586
Ama87I CYCGRG 3 cut(s) 1287, 1484, 2443
AoxI GGCC 4 cut(s) 565, 2478, 2497, 2532
ApaI GGGCCC 1 cut(s) 2501
ApeKI GCWGC 8 cut(s) 1433, 2411, 2414, 2417, 2471, 2537, 2540, 2566
ApoI RAATTY 4 cut(s) 577, 724, 1549, 2152
AspLEI GCGC 6 cut(s) 420, 882, 1102, 1327, 1494, 1903
AspS9I GGNCC 5 cut(s) 296, 365, 565, 2497, 2498
AsuC2I CCSGG 3 cut(s) 2444, 2445, 2511
AsuHPI GGTGA 4 cut(s) 208, 222, 501, 1959
AvaI CYCGRG 3 cut(s) 1287, 1484, 2443
AvaII GGWCC 2 cut(s) 296, 365
AxyI CCTNAGG 1 cut(s) 1818
BaeGI GKGCMC 2 cut(s) 2501, 2554
BaeI ACNNNNGTAYC 2 cut(s) 2543, 2576
BalI TGGCCA 1 cut(s) 2480
BamHI GGATCC 1 cut(s) 145
BanI GGYRCC 2 cut(s) 1570, 2204
BanII GRGCYC 3 cut(s) 127, 983, 2501
BbsI GAAGAC 2 cut(s) 277, 906
BbvCI CCTCAGC 1 cut(s) 2463
BbvI GCAGC 8 cut(s) 1420, 2401, 2423, 2429, 2458, 2527, 2549, 2578
BccI CCATC 8 cut(s) 77, 86, 482, 604, 862, 1681, 2240, 2463
BceAI ACGGC 3 cut(s) 820, 1746, 2365
BcgI CGANNNNNNTGC 4 cut(s) 1054, 1088, 2409, 2443
BciT130I CCWGG 8 cut(s) 432, 1191, 1998, 2061, 2190, 2319, 2352, 2547
BclI TGATCA 1 cut(s) 1024
BcnI CCSGG 3 cut(s) 2444, 2445, 2511
BfaI CTAG 1 cut(s) 849
BfmI CTRYAG 3 cut(s) 984, 1578, 2415
BfrI CTTAAG 1 cut(s) 2093
BfuAI ACCTGC 2 cut(s) 347, 2159
BglII AGATCT 2 cut(s) 998, 1067
Bme18I GGWCC 2 cut(s) 296, 365
BmeRI GACNNNNNGTC 1 cut(s) 1617
BmeT110I CYCGRG 3 cut(s) 1287, 1484, 2443
BmgT120I GGNCC 5 cut(s) 296, 365, 565, 2497, 2498
BmiI GGNNCC 8 cut(s) 147, 255, 333, 1455, 1572, 2071, 2206, 2499
BmrI ACTGGG 3 cut(s) 1770, 2151, 2566
BmsI GCATC 6 cut(s) 788, 1044, 1111, 1868, 2046, 2323
BmuI ACTGGG 3 cut(s) 1770, 2151, 2566
BoxI GACNNNNGTC 1 cut(s) 1668
BpiI GAAGAC 2 cut(s) 277, 906
BpmI CTGGAG 4 cut(s) 1212, 1443, 1449, 2082
Bpu10I CCTNAGC 2 cut(s) 1587, 2463
BpuEI CTTGAG 1 cut(s) 1600
BpuMI CCSGG 3 cut(s) 2444, 2445, 2511
BsaBI GATNNNNATC 2 cut(s) 144, 2250
BsaI GGTCTC 4 cut(s) 10, 128, 1252, 1604
BsaJI CCNNGG 6 cut(s) 1872, 2188, 2306, 2351, 2443, 2546
BsaXI ACNNNNNCTCC 6 cut(s) 399, 429, 1465, 1495, 2514, 2544
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 484, 1748, 2066
Bse118I RCCGGY 1 cut(s) 1057
Bse21I CCTNAGG 1 cut(s) 1818
Bse3DI GCAATG 3 cut(s) 2184, 2481, 2521
Bse8I GATNNNNATC 2 cut(s) 144, 2250
BseBI CCWGG 8 cut(s) 432, 1191, 1998, 2061, 2190, 2319, 2352, 2547
BseDI CCNNGG 6 cut(s) 1872, 2188, 2306, 2351, 2443, 2546
BseGI GGATG 6 cut(s) 69, 493, 1673, 1789, 2314, 2455
BseJI GATNNNNATC 2 cut(s) 144, 2250
BseLI CCNNNNNNNGG 4 cut(s) 60, 484, 1748, 2066
BseMI GCAATG 3 cut(s) 2184, 2481, 2521
BseRI GAGGAG 5 cut(s) 420, 1215, 1454, 1806, 2451
BseSI GKGCMC 2 cut(s) 2501, 2554
BseXI GCAGC 8 cut(s) 1420, 2401, 2423, 2429, 2458, 2527, 2549, 2578
BseYI CCCAGC 2 cut(s) 1134, 2164
BsgI GTGCAG 1 cut(s) 375
Bsh1236I CGCG 4 cut(s) 16, 227, 882, 1492
BshFI GGCC 4 cut(s) 567, 2480, 2499, 2534
BshNI GGYRCC 2 cut(s) 1570, 2204
BsiHKCI CYCGRG 3 cut(s) 1287, 1484, 2443
BsiSI CCGG 5 cut(s) 54, 1058, 1509, 2444, 2511
BslFI GGGAC 5 cut(s) 49, 1325, 2108, 2121, 2368
BslI CCNNNNNNNGG 4 cut(s) 60, 484, 1748, 2066
BsmBI CGTCTC 1 cut(s) 843
BsmFI GGGAC 5 cut(s) 49, 1325, 2108, 2121, 2368
BsmI GAATGC 3 cut(s) 610, 2188, 2328
BsnI GGCC 4 cut(s) 567, 2480, 2499, 2534
Bso31I GGTCTC 4 cut(s) 10, 128, 1252, 1604
BsoBI CYCGRG 3 cut(s) 1287, 1484, 2443
Bsp120I GGGCCC 1 cut(s) 2497
Bsp1286I GDGCHC 4 cut(s) 127, 983, 2501, 2554
Bsp19I CCATGG 1 cut(s) 1872
Bsp68I TCGCGA 1 cut(s) 16
BspACI CCGC 8 cut(s) 51, 1262, 1436, 1797, 1892, 2276, 2486, 2535
BspANI GGCC 4 cut(s) 567, 2480, 2499, 2534
BspFNI CGCG 4 cut(s) 16, 227, 882, 1492
BspHI TCATGA 1 cut(s) 190
BspLI GGNNCC 8 cut(s) 147, 255, 333, 1455, 1572, 2071, 2206, 2499
BspMAI CTGCAG 3 cut(s) 988, 1582, 2419
BspMI ACCTGC 2 cut(s) 347, 2159
BspPI GGATC 6 cut(s) 140, 153, 815, 1564, 1700, 1733
BspQI GCTCTTC 1 cut(s) 723
BspT107I GGYRCC 2 cut(s) 1570, 2204
BspTI CTTAAG 1 cut(s) 2093
BspTNI GGTCTC 4 cut(s) 10, 128, 1252, 1604
BsrDI GCAATG 3 cut(s) 2184, 2481, 2521
BsrFI RCCGGY 1 cut(s) 1057
BssAI RCCGGY 1 cut(s) 1057
BssECI CCNNGG 6 cut(s) 1872, 2188, 2306, 2351, 2443, 2546
BssNAI GTATAC 1 cut(s) 1975
BssT1I CCWWGG 2 cut(s) 1872, 2306
Bst1107I GTATAC 1 cut(s) 1975
Bst2UI CCWGG 8 cut(s) 432, 1191, 1998, 2061, 2190, 2319, 2352, 2547
Bst4CI ACNGT 9 cut(s) 912, 1091, 1310, 1355, 1411, 1575, 1670, 1850, 1867
Bst6I CTCTTC 3 cut(s) 194, 397, 723
BstAFI CTTAAG 1 cut(s) 2093
BstAPI GCANNNNNTGC 3 cut(s) 1577, 2174, 2183
BstC8I GCNNGC 4 cut(s) 504, 1284, 2392, 2469
BstDSI CCRYGG 1 cut(s) 1872
BstF5I GGATG 6 cut(s) 69, 493, 1673, 1789, 2314, 2455
BstFNI CGCG 4 cut(s) 16, 227, 882, 1492
BstHHI GCGC 6 cut(s) 420, 882, 1102, 1327, 1494, 1903
BstNI CCWGG 8 cut(s) 432, 1191, 1998, 2061, 2190, 2319, 2352, 2547
BstNSI RCATGY 4 cut(s) 1898, 2032, 2350, 2437
BstPAI GACNNNNGTC 1 cut(s) 1668
BstSFI CTRYAG 3 cut(s) 984, 1578, 2415
BstSLI GKGCMC 2 cut(s) 2501, 2554
BstUI CGCG 4 cut(s) 16, 227, 882, 1492
BstV1I GCAGC 8 cut(s) 1420, 2401, 2423, 2429, 2458, 2527, 2549, 2578
BstV2I GAAGAC 2 cut(s) 277, 906
BstX2I RGATCY 6 cut(s) 145, 820, 998, 1067, 1556, 1705
BstYI RGATCY 6 cut(s) 145, 820, 998, 1067, 1556, 1705
BstZ17I GTATAC 1 cut(s) 1975
Bsu36I CCTNAGG 1 cut(s) 1818
BsuRI GGCC 4 cut(s) 567, 2480, 2499, 2534
BtgI CCRYGG 1 cut(s) 1872
BtsCI GGATG 6 cut(s) 69, 493, 1673, 1789, 2314, 2455
BtsI GCAGTG 2 cut(s) 1501, 1587
BtsIMutI CAGTG 6 cut(s) 1005, 1096, 1501, 1587, 2139, 2208
BtuMI TCGCGA 1 cut(s) 16
BveI ACCTGC 2 cut(s) 347, 2159
Cac8I GCNNGC 4 cut(s) 504, 1284, 2392, 2469
CaiI CAGNNNCTG 2 cut(s) 824, 1586
CciI TCATGA 1 cut(s) 190
CfoI GCGC 6 cut(s) 420, 882, 1102, 1327, 1494, 1903
Cfr10I RCCGGY 1 cut(s) 1057
Cfr13I GGNCC 5 cut(s) 296, 365, 565, 2497, 2498
Cfr9I CCCGGG 1 cut(s) 2443
CseI GACGC 2 cut(s) 216, 233
Csp6I GTAC 7 cut(s) 519, 740, 938, 1877, 1909, 2239, 2452
CspCI CAANNNNNGTGG 2 cut(s) 2128, 2163
CviAII CATG 9 cut(s) 191, 830, 1873, 1895, 1922, 2029, 2347, 2366, 2434
CviQI GTAC 7 cut(s) 519, 740, 938, 1877, 1909, 2239, 2452
DriI GACNNNNNGTC 1 cut(s) 1617
EaeI YGGCCR 2 cut(s) 2478, 2532
Eam1104I CTCTTC 3 cut(s) 194, 397, 723
Eam1105I GACNNNNNGTC 1 cut(s) 1617
EarI CTCTTC 3 cut(s) 194, 397, 723
EciI GGCGGA 2 cut(s) 40, 2291
Eco130I CCWWGG 2 cut(s) 1872, 2306
Eco24I GRGCYC 3 cut(s) 127, 983, 2501
Eco31I GGTCTC 4 cut(s) 10, 128, 1252, 1604
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 2 cut(s) 296, 365
Eco57I CTGAAG 1 cut(s) 1671
Eco81I CCTNAGG 1 cut(s) 1818
Eco88I CYCGRG 3 cut(s) 1287, 1484, 2443
EcoO109I RGGNCCY 1 cut(s) 2498
EcoRII CCWGG 8 cut(s) 430, 1189, 1996, 2059, 2188, 2317, 2350, 2545
EcoRV GATATC 1 cut(s) 115
EcoT14I CCWWGG 2 cut(s) 1872, 2306
EcoT38I GRGCYC 3 cut(s) 127, 983, 2501
ErhI CCWWGG 2 cut(s) 1872, 2306
Esp3I CGTCTC 1 cut(s) 843
FaeI CATG 9 cut(s) 194, 833, 1876, 1898, 1925, 2032, 2350, 2369, 2437
FalI AAGNNNNNCTT 4 cut(s) 934, 966, 1266, 1298
FaqI GGGAC 5 cut(s) 49, 1325, 2108, 2121, 2368
FatI CATG 9 cut(s) 190, 829, 1872, 1894, 1921, 2028, 2346, 2365, 2433
FauNDI CATATG 2 cut(s) 2493, 2583
FbaI TGATCA 1 cut(s) 1024
FblI GTMKAC 2 cut(s) 1807, 1974
FokI GGATG 6 cut(s) 56, 500, 1660, 1796, 2301, 2442
FriOI GRGCYC 3 cut(s) 127, 983, 2501
FspBI CTAG 1 cut(s) 849
FspI TGCGCA 1 cut(s) 419
GlaI GCGC 6 cut(s) 419, 881, 1101, 1326, 1493, 1902
GsaI CCCAGC 2 cut(s) 1138, 2168
GsuI CTGGAG 4 cut(s) 1212, 1443, 1449, 2082
HaeIII GGCC 4 cut(s) 567, 2480, 2499, 2534
HapII CCGG 5 cut(s) 54, 1058, 1509, 2444, 2511
HgaI GACGC 2 cut(s) 216, 233
HhaI GCGC 6 cut(s) 420, 882, 1102, 1327, 1494, 1903
Hin1II CATG 9 cut(s) 194, 833, 1876, 1898, 1925, 2032, 2350, 2369, 2437
Hin6I GCGC 6 cut(s) 418, 880, 1100, 1325, 1492, 1901
HinP1I GCGC 6 cut(s) 418, 880, 1100, 1325, 1492, 1901
HincII GTYRAC 1 cut(s) 1473
HindII GTYRAC 1 cut(s) 1473
HindIII AAGCTT 3 cut(s) 1128, 1280, 1375
HpaII CCGG 5 cut(s) 54, 1058, 1509, 2444, 2511
HphI GGTGA 4 cut(s) 208, 222, 501, 1959
Hpy166II GTNNAC 6 cut(s) 428, 816, 1473, 1808, 1835, 1975
Hpy8I GTNNAC 6 cut(s) 428, 816, 1473, 1808, 1835, 1975
HpyAV CCTTC 4 cut(s) 17, 214, 556, 1695
HpyCH4III ACNGT 9 cut(s) 912, 1091, 1310, 1355, 1411, 1575, 1670, 1850, 1867
HpyCH4IV ACGT 2 cut(s) 660, 1977
HpySE526I ACGT 2 cut(s) 660, 1977
Hsp92II CATG 9 cut(s) 194, 833, 1876, 1898, 1925, 2032, 2350, 2369, 2437
HspAI GCGC 6 cut(s) 418, 880, 1100, 1325, 1492, 1901
Ksp22I TGATCA 1 cut(s) 1024
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 6 cut(s) 331, 1430, 1486, 2311, 2374, 2548
Lsp1109I GCAGC 8 cut(s) 1420, 2401, 2423, 2429, 2458, 2527, 2549, 2578
LweI GCATC 6 cut(s) 788, 1044, 1111, 1868, 2046, 2323
MaeI CTAG 1 cut(s) 849
MaeII ACGT 2 cut(s) 660, 1977
MaeIII GTNAC 3 cut(s) 228, 1349, 1716
MboII GAAGA 7 cut(s) 211, 282, 414, 710, 906, 2231, 2418
MflI RGATCY 6 cut(s) 145, 820, 998, 1067, 1556, 1705
MhlI GDGCHC 4 cut(s) 127, 983, 2501, 2554
MlsI TGGCCA 1 cut(s) 2480
MluI ACGCGT 1 cut(s) 225
MluNI TGGCCA 1 cut(s) 2480
MlyI GAGTC 6 cut(s) 31, 1203, 1262, 1347, 1496, 2208
MmeI TCCRAC 3 cut(s) 877, 2179, 2500
Mox20I TGGCCA 1 cut(s) 2480
MscI TGGCCA 1 cut(s) 2480
MseI TTAA 6 cut(s) 498, 876, 1413, 2094, 2115, 2406
MslI CAYNNNNRTG 2 cut(s) 514, 2079
Msp20I TGGCCA 1 cut(s) 2480
MspA1I CMGCKG 3 cut(s) 935, 2414, 2540
MspCI CTTAAG 1 cut(s) 2093
MspI CCGG 5 cut(s) 54, 1058, 1509, 2444, 2511
Mva1269I GAATGC 3 cut(s) 610, 2188, 2328
MvaI CCWGG 8 cut(s) 432, 1191, 1998, 2061, 2190, 2319, 2352, 2547
MvnI CGCG 4 cut(s) 16, 227, 882, 1492
NciI CCSGG 3 cut(s) 2444, 2445, 2511
NcoI CCATGG 1 cut(s) 1872
NdeI CATATG 2 cut(s) 2493, 2583
NlaIII CATG 9 cut(s) 194, 833, 1876, 1898, 1925, 2032, 2350, 2369, 2437
NlaIV GGNNCC 8 cut(s) 147, 255, 333, 1455, 1572, 2071, 2206, 2499
NmuCI GTSAC 2 cut(s) 228, 1349
NruI TCGCGA 1 cut(s) 16
NsbI TGCGCA 1 cut(s) 419
NspI RCATGY 4 cut(s) 1898, 2032, 2350, 2437
PagI TCATGA 1 cut(s) 190
PciI ACATGT 1 cut(s) 2028
PciSI GCTCTTC 1 cut(s) 723
PctI GAATGC 3 cut(s) 610, 2188, 2328
PflFI GACNNNGTC 1 cut(s) 1803
PflMI CCANNNNNTGG 1 cut(s) 484
PfoI TCCNGGA 2 cut(s) 1189, 2317
PleI GAGTC 6 cut(s) 31, 1202, 1261, 1346, 1495, 2208
PpsI GAGTC 6 cut(s) 31, 1202, 1261, 1346, 1495, 2208
PscI ACATGT 1 cut(s) 2028
PshAI GACNNNNGTC 1 cut(s) 1668
Psp1406I AACGTT 1 cut(s) 660
Psp6I CCWGG 8 cut(s) 430, 1189, 1996, 2059, 2188, 2317, 2350, 2545
PspFI CCCAGC 2 cut(s) 1134, 2164
PspGI CCWGG 8 cut(s) 430, 1189, 1996, 2059, 2188, 2317, 2350, 2545
PspN4I GGNNCC 8 cut(s) 147, 255, 333, 1455, 1572, 2071, 2206, 2499
PspOMI GGGCCC 1 cut(s) 2497
PspPI GGNCC 5 cut(s) 296, 365, 565, 2497, 2498
PstI CTGCAG 3 cut(s) 988, 1582, 2419
PstNI CAGNNNCTG 2 cut(s) 824, 1586
PsuI RGATCY 6 cut(s) 145, 820, 998, 1067, 1556, 1705
PsyI GACNNNGTC 1 cut(s) 1803
PvuII CAGCTG 3 cut(s) 935, 2414, 2540
RruI TCGCGA 1 cut(s) 16
RsaI GTAC 7 cut(s) 520, 741, 939, 1878, 1910, 2240, 2453
RsaNI GTAC 7 cut(s) 519, 740, 938, 1877, 1909, 2239, 2452
RseI CAYNNNNRTG 2 cut(s) 514, 2079
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 6 cut(s) 498, 876, 1413, 2094, 2115, 2406
Sau96I GGNCC 5 cut(s) 296, 365, 565, 2497, 2498
SchI GAGTC 6 cut(s) 31, 1203, 1262, 1347, 1496, 2208
SduI GDGCHC 4 cut(s) 127, 983, 2501, 2554
SfaNI GCATC 6 cut(s) 788, 1044, 1111, 1868, 2046, 2323
SfcI CTRYAG 3 cut(s) 984, 1578, 2415
SinI GGWCC 2 cut(s) 296, 365
SmaI CCCGGG 1 cut(s) 2445
SmiMI CAYNNNNRTG 2 cut(s) 514, 2079
SmlI CTYRAG 2 cut(s) 1615, 2093
SmoI CTYRAG 2 cut(s) 1615, 2093
SsiI CCGC 8 cut(s) 51, 1262, 1436, 1797, 1892, 2276, 2486, 2535
SspI AATATT 1 cut(s) 1333
SspMI CTAG 1 cut(s) 849
StyI CCWWGG 2 cut(s) 1872, 2306
TaaI ACNGT 9 cut(s) 912, 1091, 1310, 1355, 1411, 1575, 1670, 1850, 1867
TaiI ACGT 2 cut(s) 663, 1980
TaqI TCGA 4 cut(s) 21, 646, 1660, 2217
TaqII GACCGA 1 cut(s) 152
TatI WGTACW 2 cut(s) 518, 2238
TauI GCSGC 2 cut(s) 1265, 2537
Tru1I TTAA 6 cut(s) 498, 876, 1413, 2094, 2115, 2406
Tru9I TTAA 6 cut(s) 498, 876, 1413, 2094, 2115, 2406
TscAI CASTG 6 cut(s) 1012, 1096, 1501, 1587, 2146, 2215
TseFI GTSAC 2 cut(s) 228, 1349
TseI GCWGC 8 cut(s) 1433, 2411, 2414, 2417, 2471, 2537, 2540, 2566
Tsp45I GTSAC 2 cut(s) 228, 1349
TspMI CCCGGG 1 cut(s) 2443
TspRI CASTG 6 cut(s) 1012, 1096, 1501, 1587, 2146, 2215
Tth111I GACNNNGTC 1 cut(s) 1803
Van91I CCANNNNNTGG 1 cut(s) 484
Vha464I CTTAAG 1 cut(s) 2093
VpaK11BI GGWCC 2 cut(s) 296, 365
XapI RAATTY 4 cut(s) 577, 724, 1549, 2152
XbaI TCTAGA 1 cut(s) 848
XceI RCATGY 4 cut(s) 1898, 2032, 2350, 2437
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XmaI CCCGGG 1 cut(s) 2443
XmiI GTMKAC 2 cut(s) 1807, 1974
XspI CTAG 1 cut(s) 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.