RchiOBHm_Chr2g0157821

Belongs to the protein disulfide isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014581
Physical Location & Seq
Reverse (-)
74170996 .. 74174858
3863 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1314 bp
ATGAGAATGAGAGAGAATCAATTTCGAGCTGTACCAATCTTCTTCTTCTTGTTCTTCTTCGTCTTCAATGGCTGTGATGCGCTTTACGGACCGTCATCACCAGTGCTTCAGCTGACTCCCACCAACTTCAAGTCCAAGGTTCTGGACTCGAACCGAGTTGTTCTGGTTGAATTCTTTGCGCCGTGGTGTGGACATTGTCAAGCTCTGACTCCCATATGGGAGAAAGCCGCTACTGTGTTGAAGGGTGTGGCAACCGTAGCTGCCCTTGATGCTGATGCGCACAAGTCCCTTGCTCAGGAATATGGGATTAGAGGATTCCCGACAATAAAGGTGTTCGTACCTGGACAGCCTCCCGTTGATTATCAGGGAGCGAGAGAAGTGAAACCCATTGCAGAATTTGCAGTCAAACAGATTAAGGCACTATTGAAGGACCGCTTAAGTGGAAAAGCAACTGGAGGATCAAGTGAAAAACCTGAACCTAATGCTTCAGTAGAACTAAATTCCCAGAATTTTGATGAATTAGTTGTTAAAAGCAAAGATCTCTGGGTTGTGGAGTTTTTTGCGCCTTGGTGCGGGCACTGTAAAAAATTGGCACCTGAGTGGAAGAAGGCTGCCAAAAACTTGAAGGGAAAGGTGAAGTTGGGACACGTTGACTGTGATGCAGAGAAGTCTCTTATGAGCAGGTTCAATGTTCAAGGATTCCCAACAATATTGGTATTTGGAGCTGACAAAGATAGCCCAGTACCTTATGAGGGTGCGAGGACTGCTTCAGCAATTGAATCATTTTCTCTCGAACAACTAGAAACAAATGTTGCACCTCCTGAAGTGACTGAGCTAACTGGCCCTGATGTATTGGAAGAGAAATGTGGTTCGGCCGCCATCTGTTTTGTGGCTTTTCTACCTGATATCTTGGATTCCAAGGCAGAAGGGAGGAACAAGTACCTTCAGCAGTTATTATCTGTTGCAGAGAAGTTCAAGAAGAGTCCGTATAGCTATGTCTGGGCAGCAGCTGGTAAGCAGCAAGACCTCGAGAATCGCGTAGGTGTTGGTGGTTATGGATATCCAGCATTGGTTGCCTTAAATGTGAAAAAAGGTGCTTATGCCCCACTTAAGAGTGCCTTCGAGCTTGATCAGATCACCGAATTTGTGAGAGAAGCTGGGCGTGGTGGAAAGGGAAACTTGCCTCTGAACGGCACCCCTAACATTGTGAAGAGCGAACCATGGGATGGTAAAGACGGAGAGATCATTGAAGAGGATGAGTTCTCCCTTGACGAACTGATGGGGGAAGATACTAAGAGCAAGGATGAATTGTGA

Protein Analysis

437

Amino Acids

47.97

Weight (kDa)

5.57

Isoelectric Point (pI)

30.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 35 - 133 1.1e-29 Thioredoxin
Thioredoxin PF00085 164 - 262 1.2e-30 Thioredoxin
Thioredoxin_2 PF13098 180 - 253 2.7e-06 Thioredoxin-like domain
Thioredoxin_6 PF13848 197 - 384 1.4e-10 Thioredoxin-like domain
Thioredox_PDIA6_C PF24541 270 - 384 3.2e-09 PDIA6-like, C-terminal thioredoxin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014581)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04980 AT2G32920
fragaria_vesca FvH4_6g41940
malus_domestica MD09G1116100.v1.1 MD17G1107800.v1.1
prunus_persica Prupe.3G208600_v2.0.a1
pyrus_communis pycom09g04050
rosa_chinensis RchiOBHm_Chr2g0157821
rosa_laevigata RLG00000021023
rosa_multiflora Rmu_sc0002441.1_g000014
rosa_roxburghii Rroxscaffold_2G00091920
rosa_rugosa Rorug02G0464000
rosa_samantha Rh2AG529300 Rh2BG543400 Rh2CG513600 Rh2DG552200
rosa_wichuraiana Rw2G043760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 672
AccB1I GGYRCC 2 cut(s) 592, 1193
AccB7I CCANNNNNTGG 1 cut(s) 1226
AccII CGCG 1 cut(s) 1038
AciI CCGC 4 cut(s) 228, 433, 573, 876
AclWI GGATC 1 cut(s) 466
AcoI YGGCCR 1 cut(s) 873
AcsI RAATTY 5 cut(s) 170, 395, 499, 508, 1142
AcuI CTGAAG 5 cut(s) 92, 471, 753, 843, 929
AfaI GTAC 4 cut(s) 33, 339, 744, 941
AfiI CCNNNNNNNGG 6 cut(s) 188, 295, 572, 752, 1190, 1226
AflII CTTAAG 2 cut(s) 436, 1109
AflIII ACRYGT 1 cut(s) 646
AjnI CCWGG 1 cut(s) 340
AleI CACNNNNGTG 1 cut(s) 598
Alw26I GTCTC 1 cut(s) 675
AlwI GGATC 1 cut(s) 466
AlwNI CAGNNNCTG 1 cut(s) 1010
Ama87I CYCGRG 1 cut(s) 1028
AoxI GGCC 2 cut(s) 841, 873
ApeKI GCWGC 5 cut(s) 260, 611, 1004, 1007, 1018
ApoI RAATTY 5 cut(s) 170, 395, 499, 508, 1142
AspLEI GCGC 4 cut(s) 82, 181, 280, 565
AspS9I GGNCC 3 cut(s) 89, 430, 842
AsuHPI GGTGA 3 cut(s) 90, 646, 1129
AvaI CYCGRG 1 cut(s) 1028
AvaII GGWCC 2 cut(s) 89, 430
BaeGI GKGCMC 1 cut(s) 579
BanI GGYRCC 2 cut(s) 592, 1193
BbsI GAAGAC 1 cut(s) 55
BbvI GCAGC 5 cut(s) 247, 598, 1016, 1019, 1030
BccI CCATC 3 cut(s) 887, 1220, 1273
BceAI ACGGC 2 cut(s) 166, 1207
BciT130I CCWGG 1 cut(s) 342
BclI TGATCA 1 cut(s) 1129
BcoDI GTCTC 1 cut(s) 675
BfaI CTAG 1 cut(s) 800
BfrI CTTAAG 2 cut(s) 436, 1109
BfuAI ACCTGC 1 cut(s) 672
BglII AGATCT 1 cut(s) 538
BisI GCNGC 7 cut(s) 228, 261, 612, 876, 1005, 1008, 1019
BlsI GCNGC 7 cut(s) 229, 262, 613, 877, 1006, 1009, 1020
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 2 cut(s) 89, 430
BmeT110I CYCGRG 1 cut(s) 1028
BmgT120I GGNCC 3 cut(s) 89, 430, 842
BmiI GGNNCC 2 cut(s) 594, 1195
BmrFI CCNGG 1 cut(s) 342
BmrI ACTGGG 1 cut(s) 734
BmsI GCATC 4 cut(s) 67, 259, 265, 649
BmuI ACTGGG 1 cut(s) 734
BpiI GAAGAC 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 474
Bpu10I CCTNAGC 1 cut(s) 294
BsaJI CCNNGG 5 cut(s) 135, 182, 566, 918, 1220
Bsc4I CCNNNNNNNGG 6 cut(s) 188, 295, 572, 752, 1190, 1226
Bse1I ACTGG 4 cut(s) 101, 457, 740, 844
Bse3DI GCAATG 1 cut(s) 387
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 5 cut(s) 135, 182, 566, 918, 1220
BseGI GGATG 3 cut(s) 1231, 1261, 1309
BseLI CCNNNNNNNGG 6 cut(s) 188, 295, 572, 752, 1190, 1226
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 3 cut(s) 308, 588, 822
BseNI ACTGG 4 cut(s) 101, 457, 740, 844
BseSI GKGCMC 1 cut(s) 579
BseX3I CGGCCG 1 cut(s) 873
BseXI GCAGC 5 cut(s) 247, 598, 1016, 1019, 1030
BseYI CCCAGC 1 cut(s) 1157
Bsh1236I CGCG 1 cut(s) 1038
Bsh1285I CGRYCG 1 cut(s) 876
BshFI GGCC 2 cut(s) 843, 875
BshNI GGYRCC 2 cut(s) 592, 1193
BsiEI CGRYCG 1 cut(s) 876
BsiHKCI CYCGRG 1 cut(s) 1028
BslFI GGGAC 2 cut(s) 271, 657
BslI CCNNNNNNNGG 6 cut(s) 188, 295, 572, 752, 1190, 1226
BsmAI GTCTC 1 cut(s) 675
BsmFI GGGAC 2 cut(s) 271, 657
BsnI GGCC 2 cut(s) 843, 875
BsoBI CYCGRG 1 cut(s) 1028
Bsp1286I GDGCHC 1 cut(s) 579
Bsp143I GATC 5 cut(s) 458, 538, 1129, 1134, 1242
Bsp19I CCATGG 1 cut(s) 1220
BspACI CCGC 4 cut(s) 228, 433, 573, 876
BspANI GGCC 2 cut(s) 843, 875
BspCNI CTCAG 3 cut(s) 307, 589, 823
BspFNI CGCG 1 cut(s) 1038
BspLI GGNNCC 2 cut(s) 594, 1195
BspMI ACCTGC 1 cut(s) 672
BspPI GGATC 1 cut(s) 466
BspQI GCTCTTC 1 cut(s) 1205
BspT107I GGYRCC 2 cut(s) 592, 1193
BspTI CTTAAG 2 cut(s) 436, 1109
BsrDI GCAATG 1 cut(s) 387
BsrI ACTGG 4 cut(s) 101, 457, 740, 844
BssECI CCNNGG 5 cut(s) 135, 182, 566, 918, 1220
BssMI GATC 5 cut(s) 458, 538, 1129, 1134, 1242
BssT1I CCWWGG 4 cut(s) 135, 566, 918, 1220
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 5 cut(s) 93, 235, 256, 581, 656
Bst6I CTCTTC 4 cut(s) 852, 974, 1205, 1245
BstAFI CTTAAG 2 cut(s) 436, 1109
BstAPI GCANNNNNTGC 2 cut(s) 398, 1073
BstC8I GCNNGC 1 cut(s) 575
BstDEI CTNAG 4 cut(s) 294, 597, 831, 1293
BstDSI CCRYGG 2 cut(s) 182, 1220
BstENI CCTNNNNNAGG 1 cut(s) 293
BstF5I GGATG 3 cut(s) 1231, 1261, 1309
BstFNI CGCG 1 cut(s) 1038
BstHHI GCGC 4 cut(s) 82, 181, 280, 565
BstKTI GATC 5 cut(s) 461, 541, 1132, 1137, 1245
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 5 cut(s) 458, 538, 1129, 1134, 1242
BstMCI CGRYCG 1 cut(s) 876
BstMWI GCNNNNNNNGC 5 cut(s) 257, 269, 398, 764, 1073
BstNI CCWGG 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 340
BstSLI GKGCMC 1 cut(s) 579
BstUI CGCG 1 cut(s) 1038
BstV1I GCAGC 5 cut(s) 247, 598, 1016, 1019, 1030
BstV2I GAAGAC 1 cut(s) 55
BstX2I RGATCY 1 cut(s) 538
BstXI CCANNNNNNTGG 1 cut(s) 142
BstYI RGATCY 1 cut(s) 538
BstZI CGGCCG 1 cut(s) 873
BsuRI GGCC 2 cut(s) 843, 875
BtgI CCRYGG 2 cut(s) 182, 1220
BtsCI GGATG 3 cut(s) 1231, 1261, 1309
BtsIMutI CAGTG 2 cut(s) 108, 577
BveI ACCTGC 1 cut(s) 672
Cac8I GCNNGC 1 cut(s) 575
CaiI CAGNNNCTG 1 cut(s) 1010
CfoI GCGC 4 cut(s) 82, 181, 280, 565
Cfr13I GGNCC 3 cut(s) 89, 430, 842
CpoI CGGWCCG 1 cut(s) 89
Csp6I GTAC 4 cut(s) 32, 338, 743, 940
CspI CGGWCCG 1 cut(s) 89
CviAII CATG 1 cut(s) 1221
CviQI GTAC 4 cut(s) 32, 338, 743, 940
DdeI CTNAG 4 cut(s) 294, 597, 831, 1293
DpnI GATC 5 cut(s) 460, 540, 1131, 1136, 1244
DpnII GATC 5 cut(s) 458, 538, 1129, 1134, 1242
EaeI YGGCCR 1 cut(s) 873
EagI CGGCCG 1 cut(s) 873
Eam1104I CTCTTC 4 cut(s) 852, 974, 1205, 1245
EarI CTCTTC 4 cut(s) 852, 974, 1205, 1245
EclXI CGGCCG 1 cut(s) 873
Eco130I CCWWGG 4 cut(s) 135, 566, 918, 1220
Eco32I GATATC 2 cut(s) 907, 1061
Eco47I GGWCC 2 cut(s) 89, 430
Eco52I CGGCCG 1 cut(s) 873
Eco57I CTGAAG 5 cut(s) 92, 471, 753, 843, 929
Eco88I CYCGRG 1 cut(s) 1028
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 340
EcoRV GATATC 2 cut(s) 907, 1061
EcoT14I CCWWGG 4 cut(s) 135, 566, 918, 1220
ErhI CCWWGG 4 cut(s) 135, 566, 918, 1220
FaeI CATG 1 cut(s) 1224
FalI AAGNNNNNCTT 6 cut(s) 419, 451, 1103, 1135, 1163, 1195
FaqI GGGAC 2 cut(s) 271, 657
FatI CATG 1 cut(s) 1220
FauI CCCGC 1 cut(s) 566
FauNDI CATATG 1 cut(s) 215
FbaI TGATCA 1 cut(s) 1129
Fnu4HI GCNGC 7 cut(s) 228, 261, 612, 876, 1005, 1008, 1019
FokI GGATG 2 cut(s) 1238, 1268
Fsp4HI GCNGC 7 cut(s) 228, 261, 612, 876, 1005, 1008, 1019
FspAI RTGCGCAY 1 cut(s) 279
FspBI CTAG 1 cut(s) 800
FspI TGCGCA 1 cut(s) 279
GlaI GCGC 4 cut(s) 81, 180, 279, 564
GluI GCNGC 7 cut(s) 228, 261, 612, 876, 1005, 1008, 1019
GsaI CCCAGC 1 cut(s) 1161
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 2 cut(s) 843, 875
HhaI GCGC 4 cut(s) 82, 181, 280, 565
Hin1II CATG 1 cut(s) 1224
Hin6I GCGC 4 cut(s) 80, 179, 278, 563
HinP1I GCGC 4 cut(s) 80, 179, 278, 563
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HphI GGTGA 3 cut(s) 90, 646, 1129
Hpy166II GTNNAC 2 cut(s) 191, 652
Hpy188I TCNGA 3 cut(s) 207, 1134, 1188
Hpy188III TCNNGA 7 cut(s) 143, 296, 319, 791, 821, 976, 1030
Hpy8I GTNNAC 2 cut(s) 191, 652
HpyAV CCTTC 7 cut(s) 235, 421, 601, 619, 920, 953, 1129
HpyCH4III ACNGT 5 cut(s) 93, 235, 256, 581, 656
HpyCH4IV ACGT 1 cut(s) 648
HpyCH4V TGCA 5 cut(s) 392, 401, 662, 815, 965
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 269, 398, 764, 1073
HpyF3I CTNAG 4 cut(s) 294, 597, 831, 1293
HpySE526I ACGT 1 cut(s) 648
Hsp92II CATG 1 cut(s) 1224
HspAI GCGC 4 cut(s) 80, 179, 278, 563
Ksp22I TGATCA 1 cut(s) 1129
Kzo9I GATC 5 cut(s) 458, 538, 1129, 1134, 1242
LguI GCTCTTC 1 cut(s) 1205
LmnI GCTCC 2 cut(s) 368, 722
Lsp1109I GCAGC 5 cut(s) 247, 598, 1016, 1019, 1030
LweI GCATC 4 cut(s) 67, 259, 265, 649
MaeI CTAG 1 cut(s) 800
MaeII ACGT 1 cut(s) 648
MaeIII GTNAC 1 cut(s) 826
MalI GATC 5 cut(s) 460, 540, 1131, 1136, 1244
MboI GATC 5 cut(s) 458, 538, 1129, 1134, 1242
MfeI CAATTG 1 cut(s) 774
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 1 cut(s) 579
MlyI GAGTC 4 cut(s) 109, 140, 202, 991
MseI TTAA 5 cut(s) 414, 437, 528, 1079, 1110
MslI CAYNNNNRTG 1 cut(s) 598
MspA1I CMGCKG 2 cut(s) 112, 1010
MspCI CTTAAG 2 cut(s) 436, 1109
MspR9I CCNGG 1 cut(s) 342
MunI CAATTG 1 cut(s) 774
MvaI CCWGG 1 cut(s) 342
MvnI CGCG 1 cut(s) 1038
MwoI GCNNNNNNNGC 5 cut(s) 257, 269, 398, 764, 1073
NcoI CCATGG 1 cut(s) 1220
NdeI CATATG 1 cut(s) 215
NdeII GATC 5 cut(s) 458, 538, 1129, 1134, 1242
NlaIII CATG 1 cut(s) 1224
NlaIV GGNNCC 2 cut(s) 594, 1195
NmuCI GTSAC 1 cut(s) 826
NsbI TGCGCA 1 cut(s) 279
OliI CACNNNNGTG 1 cut(s) 598
PaeR7I CTCGAG 1 cut(s) 1028
PciSI GCTCTTC 1 cut(s) 1205
PcsI WCGNNNNNNNCGW 1 cut(s) 1035
PfeI GAWTC 6 cut(s) 16, 315, 699, 779, 914, 1033
PflFI GACNNNGTC 1 cut(s) 195
PflMI CCANNNNNTGG 1 cut(s) 1226
PkrI GCNGC 7 cut(s) 229, 262, 613, 877, 1006, 1009, 1020
PleI GAGTC 4 cut(s) 109, 140, 202, 990
PpsI GAGTC 4 cut(s) 109, 140, 202, 990
Psp6I CCWGG 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 1157
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 594, 1195
PspPI GGNCC 3 cut(s) 89, 430, 842
PstNI CAGNNNCTG 1 cut(s) 1010
PsuI RGATCY 1 cut(s) 538
PsyI GACNNNGTC 1 cut(s) 195
PvuII CAGCTG 2 cut(s) 112, 1010
RsaI GTAC 4 cut(s) 33, 339, 744, 941
RsaNI GTAC 4 cut(s) 32, 338, 743, 940
RseI CAYNNNNRTG 1 cut(s) 598
Rsr2I CGGWCCG 1 cut(s) 89
RsrII CGGWCCG 1 cut(s) 89
SapI GCTCTTC 1 cut(s) 1205
SaqAI TTAA 5 cut(s) 414, 437, 528, 1079, 1110
SatI GCNGC 7 cut(s) 228, 261, 612, 876, 1005, 1008, 1019
Sau3AI GATC 5 cut(s) 458, 538, 1129, 1134, 1242
Sau96I GGNCC 3 cut(s) 89, 430, 842
SchI GAGTC 4 cut(s) 109, 140, 202, 991
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 1 cut(s) 579
SfaNI GCATC 4 cut(s) 67, 259, 265, 649
Sfr274I CTCGAG 1 cut(s) 1028
SinI GGWCC 2 cut(s) 89, 430
SlaI CTCGAG 1 cut(s) 1028
SmiMI CAYNNNNRTG 1 cut(s) 598
SmlI CTYRAG 3 cut(s) 436, 1028, 1109
SmoI CTYRAG 3 cut(s) 436, 1028, 1109
SsiI CCGC 4 cut(s) 228, 433, 573, 876
SspI AATATT 1 cut(s) 711
SspMI CTAG 1 cut(s) 800
StyD4I CCNGG 1 cut(s) 340
StyI CCWWGG 4 cut(s) 135, 566, 918, 1220
TaaI ACNGT 5 cut(s) 93, 235, 256, 581, 656
TaiI ACGT 1 cut(s) 651
TaqI TCGA 5 cut(s) 25, 149, 792, 1029, 1122
TauI GCSGC 2 cut(s) 230, 878
TfiI GAWTC 6 cut(s) 16, 315, 699, 779, 914, 1033
Tru1I TTAA 5 cut(s) 414, 437, 528, 1079, 1110
Tru9I TTAA 5 cut(s) 414, 437, 528, 1079, 1110
TscAI CASTG 2 cut(s) 108, 584
TseFI GTSAC 1 cut(s) 826
TseI GCWGC 5 cut(s) 260, 611, 1004, 1007, 1018
Tsp45I GTSAC 1 cut(s) 826
TspDTI ATGAA 1 cut(s) 531
TspGWI ACGGA 3 cut(s) 102, 975, 1251
TspRI CASTG 2 cut(s) 108, 584
Tth111I GACNNNGTC 1 cut(s) 195
Van91I CCANNNNNTGG 1 cut(s) 1226
Vha464I CTTAAG 2 cut(s) 436, 1109
VpaK11BI GGWCC 2 cut(s) 89, 430
XagI CCTNNNNNAGG 1 cut(s) 293
XapI RAATTY 5 cut(s) 170, 395, 499, 508, 1142
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XhoI CTCGAG 1 cut(s) 1028
XspI CTAG 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.