RchiOBHm_Chr2g0163031

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017350
Physical Location & Seq
Forward (+)
78695720 .. 78698191
2472 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 333 bp
ATGGATATTGATTATCAGGTACTTTATGATGCCTTTTTCAAGTACCAGACTAAGCCAAAGCTCACAGCACTTGGTGATCTGTACTATGAAGGAAAAGAGTTTAATCAGGTAAGCTTGAGGGATCAGATGAAAGACTGCGGGCTCCGAGAGCCAGTTGATCATGAGACAAAGCATTGGGGTGATTTGGAGGAAGAAGAGAGAGAAGATTTAGATGATGACATGGTTGCAGATAGTGAGAAGAAGAGGAAGCGTAAGATGAAGGAAAAGGAAGGCAAATACAACAAGAAGAAGGATTTCATTTGGAAGAGCTGTTGGGCAGCTTATCTCAAATAA

Protein Analysis

110

Amino Acids

13.34

Weight (kDa)

5.49

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF382 PF04037 1 - 33 3.4e-12 Domain of unknown function (DUF382)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017350)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45270 FvH4_6g45280
rosa_chinensis RchiOBHm_Chr2g0163031
rosa_laevigata RLG00000021366
rosa_multiflora Rmu_sc0022169.1_g000001
rosa_roxburghii Rroxscaffold_2G00087610
rosa_rugosa Rorug02G0499900
rosa_samantha Rh2AG566200 Rh2BG578500 Rh2CG548400 Rh2DG588800 Rh2DG588900
rosa_wichuraiana Rw2G046860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 138
AclWI GGATC 1 cut(s) 129
AdeI CACNNNGTG 1 cut(s) 74
AfaI GTAC 3 cut(s) 21, 44, 83
AgsI TTSAA 1 cut(s) 40
AluBI AGCT 4 cut(s) 61, 114, 309, 320
AluI AGCT 4 cut(s) 61, 114, 309, 320
Alw26I GTCTC 1 cut(s) 158
AlwI GGATC 1 cut(s) 129
ApeKI GCWGC 1 cut(s) 317
Asp700I GAANNNNTTC 1 cut(s) 293
AsuHPI GGTGA 2 cut(s) 86, 191
BanII GRGCYC 1 cut(s) 144
BbvI GCAGC 1 cut(s) 329
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 158
BisI GCNGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 319
BmiI GGNNCC 1 cut(s) 143
BmsI GCATC 1 cut(s) 19
BpuEI CTTGAG 1 cut(s) 136
Bse1I ACTGG 1 cut(s) 152
BseNI ACTGG 1 cut(s) 152
BseXI GCAGC 1 cut(s) 329
BsmAI GTCTC 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 3 cut(s) 76, 121, 157
BspACI CCGC 1 cut(s) 138
BspHI TCATGA 1 cut(s) 160
BspLI GGNNCC 1 cut(s) 143
BspPI GGATC 1 cut(s) 129
BspQI GCTCTTC 1 cut(s) 299
BsrI ACTGG 1 cut(s) 152
BssMI GATC 3 cut(s) 76, 121, 157
Bst6I CTCTTC 3 cut(s) 189, 236, 299
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 1 cut(s) 51
BstKTI GATC 3 cut(s) 79, 124, 160
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 3 cut(s) 76, 121, 157
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstV1I GCAGC 1 cut(s) 329
Cac8I GCNNGC 1 cut(s) 140
CciI TCATGA 1 cut(s) 160
Csp6I GTAC 3 cut(s) 20, 43, 82
CviAII CATG 2 cut(s) 161, 220
CviJI RGCY 7 cut(s) 55, 61, 114, 142, 151, 309, 320
CviKI_1 RGCY 7 cut(s) 55, 61, 114, 142, 151, 309, 320
CviQI GTAC 3 cut(s) 20, 43, 82
DdeI CTNAG 1 cut(s) 51
DpnI GATC 3 cut(s) 78, 123, 159
DpnII GATC 3 cut(s) 76, 121, 157
DraIII CACNNNGTG 1 cut(s) 74
Eam1104I CTCTTC 3 cut(s) 189, 236, 299
EarI CTCTTC 3 cut(s) 189, 236, 299
Eco24I GRGCYC 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 144
FaeI CATG 2 cut(s) 164, 223
FaiI YATR 4 cut(s) 27, 87, 162, 221
FatI CATG 2 cut(s) 160, 219
FauI CCCGC 1 cut(s) 131
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 318
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 1 cut(s) 318
GluI GCNGC 1 cut(s) 318
Hin1II CATG 2 cut(s) 164, 223
HindIII AAGCTT 1 cut(s) 112
HphI GGTGA 2 cut(s) 86, 191
Hpy188I TCNGA 2 cut(s) 126, 146
Hpy188III TCNNGA 1 cut(s) 161
HpyAV CCTTC 4 cut(s) 83, 253, 263, 283
HpyCH4V TGCA 1 cut(s) 227
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpyF3I CTNAG 1 cut(s) 51
Hsp92II CATG 2 cut(s) 164, 223
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 3 cut(s) 76, 121, 157
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 1 cut(s) 147
LpnPI CCDG 4 cut(s) 2, 59, 92, 165
Lsp1109I GCAGC 1 cut(s) 329
LweI GCATC 1 cut(s) 19
MalI GATC 3 cut(s) 78, 123, 159
MboI GATC 3 cut(s) 76, 121, 157
MboII GAAGA 7 cut(s) 203, 206, 215, 250, 253, 298, 316
MhlI GDGCHC 1 cut(s) 144
MnlI CCTC 3 cut(s) 111, 181, 237
MroXI GAANNNNTTC 1 cut(s) 293
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 148
NdeII GATC 3 cut(s) 76, 121, 157
NlaIII CATG 2 cut(s) 164, 223
NlaIV GGNNCC 1 cut(s) 143
PagI TCATGA 1 cut(s) 160
PciSI GCTCTTC 1 cut(s) 299
PdmI GAANNNNTTC 1 cut(s) 293
PkrI GCNGC 1 cut(s) 319
PspN4I GGNNCC 1 cut(s) 143
RsaI GTAC 3 cut(s) 21, 44, 83
RsaNI GTAC 3 cut(s) 20, 43, 82
RseI CAYNNNNRTG 1 cut(s) 177
SapI GCTCTTC 1 cut(s) 299
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 1 cut(s) 318
Sau3AI GATC 3 cut(s) 76, 121, 157
SduI GDGCHC 1 cut(s) 144
SetI ASST 6 cut(s) 21, 63, 111, 116, 311, 322
SfaNI GCATC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 177
SmlI CTYRAG 1 cut(s) 115
SmoI CTYRAG 1 cut(s) 115
SsiI CCGC 1 cut(s) 138
TatI WGTACW 1 cut(s) 81
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TseI GCWGC 1 cut(s) 317
TspDTI ATGAA 4 cut(s) 102, 143, 272, 286
XmnI GAANNNNTTC 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.