Rh2DG588800

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0017350
Physical Location & Seq
Forward (+)
82176764 .. 82177641
878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG588800.1

Sequence Viewer

Length: 741 bp
ATGAGTGACGAGACAATCTTGCACCAGCCAGCAGCGAGCCACGGTGAAGATGATGTTGAGAAACTGAGAAATTTTTTCGAAAAGTTCATGTTGGAGGAAGGGGTTGGTGTTGAGGAGTTGAGAATTCTTTTCGAAAAACTCATGTTCAGTAATGAAGCGGGTGTTGACGAGTTGAGAAAAAGTTTAGAAGAATTCTTGTTCAAGGAAGCAGCGGGTGTTGAGGAGAAAAAGGAAGATGATGAGTCCCGAGAAGAAGCTGAGGAGAATCAACCTAAACAAGATAAAACAATTGCAGCTAGATTGAAACAGAGGTGTGCATTAAGGCCTAAACTGCCGGTGCTTAATTTGAAATCGGATCGGAATACTGTACCGGTGCCAAAGCAATGGTGCGGGAAAGGGGTAGATTTGGAGGGTGAAAGTGGAATCGAGAAGCCACCATATCGGCTTCCTAGTTACATTGCTGCAACTGGAATCGAGAAAATTAGACAGGCGTGGATTGAGAAAGAAGATAGTAACAAGTTGGGGGCAATGGATATTGATTATCAGGTACTTTATGATGCCTTTTTCAAGTACCAGACTAAGCCAAAGCTCACAGCACTTGGTGATCTGTACTATGAAGGAAAAGAGTTTAATCAGGTAAGCTTGAGGGATCAGATGAAAGACTGCAGGCTCCGAGAGCCAGTTGATCATGAGACAAAGCATTGGGGTGATTTGGAGGAAGAAGAGAGAGAGAAGATTTAG

Protein Analysis

246

Amino Acids

28.57

Weight (kDa)

4.88

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF382 PF04037 109 - 209 1.2e-30 Domain of unknown function (DUF382)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017350)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45270 FvH4_6g45280
rosa_chinensis RchiOBHm_Chr2g0163031
rosa_laevigata RLG00000021366
rosa_multiflora Rmu_sc0022169.1_g000001
rosa_roxburghii Rroxscaffold_2G00087610
rosa_rugosa Rorug02G0499900
rosa_samantha Rh2AG566200 Rh2BG578500 Rh2CG548400 Rh2DG588800 Rh2DG588900
rosa_wichuraiana Rw2G046860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 373
AciI CCGC 3 cut(s) 158, 212, 390
AclWI GGATC 2 cut(s) 363, 657
AcsI RAATTY 3 cut(s) 70, 123, 191
AdeI CACNNNGTG 1 cut(s) 602
AfaI GTAC 4 cut(s) 369, 549, 572, 611
AgeI ACCGGT 1 cut(s) 370
AgsI TTSAA 4 cut(s) 202, 304, 349, 568
AluBI AGCT 4 cut(s) 257, 296, 589, 642
AluI AGCT 4 cut(s) 257, 296, 589, 642
Alw26I GTCTC 2 cut(s) 5, 686
AlwI GGATC 2 cut(s) 363, 657
Ama87I CYCGRG 1 cut(s) 246
AoxI GGCC 1 cut(s) 323
ApeKI GCWGC 4 cut(s) 32, 209, 293, 461
ApoI RAATTY 3 cut(s) 70, 123, 191
AsiGI ACCGGT 1 cut(s) 370
AsuHPI GGTGA 4 cut(s) 56, 425, 614, 719
AsuII TTCGAA 2 cut(s) 78, 132
AvaI CYCGRG 1 cut(s) 246
BanI GGYRCC 1 cut(s) 373
BbvCI CCTCAGC 1 cut(s) 258
BbvI GCAGC 4 cut(s) 44, 221, 305, 448
BclI TGATCA 1 cut(s) 685
BcoDI GTCTC 2 cut(s) 5, 686
BfaI CTAG 2 cut(s) 297, 450
BfmI CTRYAG 1 cut(s) 664
BisI GCNGC 4 cut(s) 33, 210, 294, 462
BlsI GCNGC 4 cut(s) 34, 211, 295, 463
BmeT110I CYCGRG 1 cut(s) 246
BmiI GGNNCC 2 cut(s) 375, 671
BmsI GCATC 1 cut(s) 547
Bpu10I CCTNAGC 1 cut(s) 258
Bpu14I TTCGAA 2 cut(s) 78, 132
BpuEI CTTGAG 1 cut(s) 664
BsaJI CCNNGG 1 cut(s) 40
BsaWI WCCGGW 1 cut(s) 370
Bse118I RCCGGY 2 cut(s) 334, 370
Bse1I ACTGG 2 cut(s) 472, 680
Bse3DI GCAATG 3 cut(s) 389, 456, 534
BseDI CCNNGG 1 cut(s) 40
BseMI GCAATG 3 cut(s) 389, 456, 534
BseMII CTCAG 2 cut(s) 56, 249
BseNI ACTGG 2 cut(s) 472, 680
BseRI GAGGAG 3 cut(s) 128, 236, 275
BseXI GCAGC 4 cut(s) 44, 221, 305, 448
BshFI GGCC 1 cut(s) 325
BshNI GGYRCC 1 cut(s) 373
BshTI ACCGGT 1 cut(s) 370
BsiHKCI CYCGRG 1 cut(s) 246
BsiSI CCGG 2 cut(s) 335, 371
BslFI GGGAC 1 cut(s) 229
BsmAI GTCTC 2 cut(s) 5, 686
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 1 cut(s) 325
BsoBI CYCGRG 1 cut(s) 246
Bsp119I TTCGAA 2 cut(s) 78, 132
Bsp143I GATC 4 cut(s) 355, 604, 649, 685
BspACI CCGC 3 cut(s) 158, 212, 390
BspANI GGCC 1 cut(s) 325
BspCNI CTCAG 2 cut(s) 57, 250
BspHI TCATGA 1 cut(s) 688
BspLI GGNNCC 2 cut(s) 375, 671
BspMAI CTGCAG 1 cut(s) 668
BspPI GGATC 2 cut(s) 363, 657
BspT104I TTCGAA 2 cut(s) 78, 132
BspT107I GGYRCC 1 cut(s) 373
BsrDI GCAATG 3 cut(s) 389, 456, 534
BsrFI RCCGGY 2 cut(s) 334, 370
BsrI ACTGG 2 cut(s) 472, 680
BssAI RCCGGY 2 cut(s) 334, 370
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 4 cut(s) 355, 604, 649, 685
Bst4CI ACNGT 2 cut(s) 44, 367
Bst6I CTCTTC 1 cut(s) 717
BstBI TTCGAA 2 cut(s) 78, 132
BstC8I GCNNGC 3 cut(s) 30, 37, 668
BstDEI CTNAG 3 cut(s) 65, 258, 579
BstDSI CCRYGG 1 cut(s) 40
BstKTI GATC 4 cut(s) 358, 607, 652, 688
BstMAI GTCTC 2 cut(s) 5, 686
BstMBI GATC 4 cut(s) 355, 604, 649, 685
BstMWI GCNNNNNNNGC 2 cut(s) 331, 676
BstSFI CTRYAG 1 cut(s) 664
BstV1I GCAGC 4 cut(s) 44, 221, 305, 448
BstXI CCANNNNNNTGG 1 cut(s) 384
BsuRI GGCC 1 cut(s) 325
BtgI CCRYGG 1 cut(s) 40
Cac8I GCNNGC 3 cut(s) 30, 37, 668
CciI TCATGA 1 cut(s) 688
Cfr10I RCCGGY 2 cut(s) 334, 370
Csp6I GTAC 4 cut(s) 368, 548, 571, 610
CspAI ACCGGT 1 cut(s) 370
CviAII CATG 3 cut(s) 88, 142, 689
CviQI GTAC 4 cut(s) 368, 548, 571, 610
DdeI CTNAG 3 cut(s) 65, 258, 579
DpnI GATC 4 cut(s) 357, 606, 651, 687
DpnII GATC 4 cut(s) 355, 604, 649, 685
DraIII CACNNNGTG 1 cut(s) 602
Eam1104I CTCTTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 717
Eco147I AGGCCT 1 cut(s) 325
Eco88I CYCGRG 1 cut(s) 246
EcoRI GAATTC 2 cut(s) 123, 191
FaeI CATG 3 cut(s) 91, 145, 692
FaiI YATR 6 cut(s) 89, 143, 439, 555, 615, 690
FaqI GGGAC 1 cut(s) 229
FatI CATG 3 cut(s) 87, 141, 688
FauI CCCGC 3 cut(s) 151, 205, 383
FbaI TGATCA 1 cut(s) 685
Fnu4HI GCNGC 4 cut(s) 33, 210, 294, 462
Fsp4HI GCNGC 4 cut(s) 33, 210, 294, 462
FspBI CTAG 2 cut(s) 297, 450
GluI GCNGC 4 cut(s) 33, 210, 294, 462
HaeIII GGCC 1 cut(s) 325
HapII CCGG 2 cut(s) 335, 371
Hin1II CATG 3 cut(s) 91, 145, 692
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 640
HinfI GANTC 4 cut(s) 242, 265, 423, 471
HpaII CCGG 2 cut(s) 335, 371
HphI GGTGA 4 cut(s) 56, 425, 614, 719
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 4 cut(s) 355, 360, 654, 674
Hpy188III TCNNGA 4 cut(s) 246, 427, 475, 689
Hpy8I GTNNAC 1 cut(s) 166
HpyAV CCTTC 2 cut(s) 92, 611
HpyCH4III ACNGT 2 cut(s) 44, 367
HpyCH4V TGCA 5 cut(s) 22, 293, 317, 464, 666
HpyF10VI GCNNNNNNNGC 2 cut(s) 331, 676
HpyF3I CTNAG 3 cut(s) 65, 258, 579
Hsp92II CATG 3 cut(s) 91, 145, 692
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 4 cut(s) 355, 604, 649, 685
LmnI GCTCC 1 cut(s) 675
Lsp1109I GCAGC 4 cut(s) 44, 221, 305, 448
LweI GCATC 1 cut(s) 547
MaeI CTAG 2 cut(s) 297, 450
MaeIII GTNAC 3 cut(s) 5, 452, 512
MalI GATC 4 cut(s) 357, 606, 651, 687
MboI GATC 4 cut(s) 355, 604, 649, 685
MboII GAAGA 7 cut(s) 59, 200, 245, 263, 518, 731, 734
MfeI CAATTG 1 cut(s) 288
MluCI AATT 6 cut(s) 70, 123, 191, 288, 343, 480
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 8 cut(s) 88, 106, 214, 253, 303, 403, 639, 709
MseI TTAA 3 cut(s) 320, 342, 630
MslI CAYNNNNRTG 1 cut(s) 705
MspA1I CMGCKG 1 cut(s) 212
MspI CCGG 2 cut(s) 335, 371
MunI CAATTG 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 331, 676
NdeII GATC 4 cut(s) 355, 604, 649, 685
NlaIII CATG 3 cut(s) 91, 145, 692
NlaIV GGNNCC 2 cut(s) 375, 671
NmuCI GTSAC 1 cut(s) 5
NspV TTCGAA 2 cut(s) 78, 132
PagI TCATGA 1 cut(s) 688
PceI AGGCCT 1 cut(s) 325
PfeI GAWTC 3 cut(s) 265, 423, 471
PinAI ACCGGT 1 cut(s) 370
PkrI GCNGC 4 cut(s) 34, 211, 295, 463
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
PspN4I GGNNCC 2 cut(s) 375, 671
PstI CTGCAG 1 cut(s) 668
RsaI GTAC 4 cut(s) 369, 549, 572, 611
RsaNI GTAC 4 cut(s) 368, 548, 571, 610
RseI CAYNNNNRTG 1 cut(s) 705
SaqAI TTAA 3 cut(s) 320, 342, 630
SatI GCNGC 4 cut(s) 33, 210, 294, 462
Sau3AI GATC 4 cut(s) 355, 604, 649, 685
SchI GAGTC 1 cut(s) 251
SetI ASST 8 cut(s) 259, 274, 298, 314, 549, 591, 639, 644
SfaNI GCATC 1 cut(s) 547
SfcI CTRYAG 1 cut(s) 664
SfuI TTCGAA 2 cut(s) 78, 132
SmiMI CAYNNNNRTG 1 cut(s) 705
SmlI CTYRAG 1 cut(s) 643
SmoI CTYRAG 1 cut(s) 643
Sse9I AATT 6 cut(s) 70, 123, 191, 288, 343, 480
SseBI AGGCCT 1 cut(s) 325
SsiI CCGC 3 cut(s) 158, 212, 390
SspMI CTAG 2 cut(s) 297, 450
StuI AGGCCT 1 cut(s) 325
TaaI ACNGT 2 cut(s) 44, 367
TaqI TCGA 4 cut(s) 78, 132, 426, 474
TasI AATT 6 cut(s) 70, 123, 191, 288, 343, 480
TatI WGTACW 1 cut(s) 609
TfiI GAWTC 3 cut(s) 265, 423, 471
Tru1I TTAA 3 cut(s) 320, 342, 630
Tru9I TTAA 3 cut(s) 320, 342, 630
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 4 cut(s) 32, 209, 293, 461
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 4 cut(s) 76, 168, 630, 671
XapI RAATTY 3 cut(s) 70, 123, 191
XspI CTAG 2 cut(s) 297, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.