RLG00000021366

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017350
Physical Location & Seq
Forward (+)
74578631 .. 74579467
837 bp
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UTR
Exon/CDS
Intron
RLM00000021366

Sequence Viewer

Length: 837 bp
ATGGGTGACGAGACAATCTTGCACCAGCCAGCAGTGAGCCACGGTGATGATGATGTTGAGAAACTGAGAAATTTTTTCGAAAAGTTCATGTTGGAGGAAGGGGTTGGTGTTGAGGAGTTGAGAATTCTTTTCGAAAAACTCATGTTCAGCAATGAAGGAGCGGGTGTTGACGAGTTGAGAAAAAGTTTAGAAGAATTCTTGTTCAAGGAAGCAGCGGGTGTTGAGGAGAAACAGCAAGATGATGAGTCCCGAGAAGAAGCCGAGGAGAATCAACAAAAACAAGAGAAAACAATTGCAGCTAGATTGAAACAGAGGTGTGCAATAAGGCCTAAACTGCCCGTGCTTAATTTGAAATCGGATCGGAATACTGTACCGGTGCCAAAGCAATGGTGCGAGAAAGGGGTAGATTTGGAGGGTGAAAGTGGAATCGAGAAGCCACCTTATCGGCTTCCTAGTTACATTGCTGCAACTGGAATCGAGAAAATTAGACAGGCGTGGATTGAGAAAGAAGATAGTAACAAGTTGGGGGCAATGGATATTGATTATCAGGTACTTTATGATGCCTTTTTCAAGTACCAGACTAAGCCAAAGCTCACAGCACTTGGCGATCTGTACTACGAAGGAAAAGAGTTTAATAAGGTAAGCTTGAGGGATCAGATGAAAGACTGCGGGCTCCGAGAGCCAGTTGATCATGAGACAAAGCATTGGGGTGATTTGGAGGAAGAAGAGGGAGAAGATTTAGATGATGACGTGGTTGCAGATAGTGAGAAGAAGAGGAAGCGTAAGATGAAGGAAAAGGAAGGCAAATACAACAAGAAGAAGGATTTCATGTTCTAA

Protein Analysis

279

Amino Acids

32.32

Weight (kDa)

4.95

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF382 PF04037 110 - 210 8.3e-31 Domain of unknown function (DUF382)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017350)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45270 FvH4_6g45280
rosa_chinensis RchiOBHm_Chr2g0163031
rosa_laevigata RLG00000021366
rosa_multiflora Rmu_sc0022169.1_g000001
rosa_roxburghii Rroxscaffold_2G00087610
rosa_rugosa Rorug02G0499900
rosa_samantha Rh2AG566200 Rh2BG578500 Rh2CG548400 Rh2DG588800 Rh2DG588900
rosa_wichuraiana Rw2G046860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 376
AccBSI CCGCTC 1 cut(s) 161
AciI CCGC 3 cut(s) 161, 215, 669
AclWI GGATC 2 cut(s) 366, 660
AcsI RAATTY 3 cut(s) 70, 123, 194
AfaI GTAC 4 cut(s) 372, 552, 575, 614
AgeI ACCGGT 1 cut(s) 373
AgsI TTSAA 4 cut(s) 205, 307, 352, 571
AjiI CACGTC 1 cut(s) 751
AloI GAACNNNNNNTCC 1 cut(s) 815
AluBI AGCT 3 cut(s) 299, 592, 645
AluI AGCT 3 cut(s) 299, 592, 645
Alw26I GTCTC 2 cut(s) 5, 689
AlwI GGATC 2 cut(s) 366, 660
Ama87I CYCGRG 1 cut(s) 249
AoxI GGCC 1 cut(s) 326
ApeKI GCWGC 3 cut(s) 212, 296, 464
ApoI RAATTY 3 cut(s) 70, 123, 194
AsiGI ACCGGT 1 cut(s) 373
Asp700I GAANNNNTTC 1 cut(s) 824
AsuHPI GGTGA 4 cut(s) 17, 56, 428, 722
AsuII TTCGAA 2 cut(s) 78, 132
AvaI CYCGRG 1 cut(s) 249
BanI GGYRCC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 675
BbvI GCAGC 3 cut(s) 224, 308, 451
BclI TGATCA 1 cut(s) 688
BcoDI GTCTC 2 cut(s) 5, 689
BfaI CTAG 2 cut(s) 300, 453
BisI GCNGC 3 cut(s) 213, 297, 465
BlsI GCNGC 3 cut(s) 214, 298, 466
BmeT110I CYCGRG 1 cut(s) 249
BmgBI CACGTC 1 cut(s) 751
BmiI GGNNCC 2 cut(s) 378, 674
BmsI GCATC 1 cut(s) 550
Bpu14I TTCGAA 2 cut(s) 78, 132
BpuEI CTTGAG 1 cut(s) 667
BsaJI CCNNGG 2 cut(s) 40, 261
BsaWI WCCGGW 1 cut(s) 373
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bse118I RCCGGY 1 cut(s) 373
Bse1I ACTGG 2 cut(s) 475, 683
Bse3DI GCAATG 4 cut(s) 157, 392, 459, 537
BseDI CCNNGG 2 cut(s) 40, 261
BseMI GCAATG 4 cut(s) 157, 392, 459, 537
BseMII CTCAG 1 cut(s) 56
BseNI ACTGG 2 cut(s) 475, 683
BseRI GAGGAG 3 cut(s) 128, 239, 278
BseXI GCAGC 3 cut(s) 224, 308, 451
BshFI GGCC 1 cut(s) 328
BshNI GGYRCC 1 cut(s) 376
BshTI ACCGGT 1 cut(s) 373
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 1 cut(s) 374
BslFI GGGAC 1 cut(s) 232
BsmAI GTCTC 2 cut(s) 5, 689
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 1 cut(s) 328
BsoBI CYCGRG 1 cut(s) 249
Bsp119I TTCGAA 2 cut(s) 78, 132
Bsp1286I GDGCHC 1 cut(s) 675
Bsp143I GATC 4 cut(s) 358, 607, 652, 688
BspACI CCGC 3 cut(s) 161, 215, 669
BspANI GGCC 1 cut(s) 328
BspCNI CTCAG 1 cut(s) 57
BspHI TCATGA 1 cut(s) 691
BspLI GGNNCC 2 cut(s) 378, 674
BspPI GGATC 2 cut(s) 366, 660
BspT104I TTCGAA 2 cut(s) 78, 132
BspT107I GGYRCC 1 cut(s) 376
BsrBI CCGCTC 1 cut(s) 161
BsrDI GCAATG 4 cut(s) 157, 392, 459, 537
BsrFI RCCGGY 1 cut(s) 373
BsrI ACTGG 2 cut(s) 475, 683
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 2 cut(s) 40, 261
BssMI GATC 4 cut(s) 358, 607, 652, 688
Bst4CI ACNGT 2 cut(s) 44, 370
Bst6I CTCTTC 2 cut(s) 720, 767
BstBI TTCGAA 2 cut(s) 78, 132
BstC8I GCNNGC 2 cut(s) 30, 671
BstDEI CTNAG 2 cut(s) 65, 582
BstDSI CCRYGG 1 cut(s) 40
BstKTI GATC 4 cut(s) 361, 610, 655, 691
BstMAI GTCTC 2 cut(s) 5, 689
BstMBI GATC 4 cut(s) 358, 607, 652, 688
BstMWI GCNNNNNNNGC 2 cut(s) 334, 679
BstV1I GCAGC 3 cut(s) 224, 308, 451
BstXI CCANNNNNNTGG 1 cut(s) 387
BsuRI GGCC 1 cut(s) 328
BtgI CCRYGG 1 cut(s) 40
BtrI CACGTC 1 cut(s) 751
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 2 cut(s) 30, 671
CciI TCATGA 1 cut(s) 691
Cfr10I RCCGGY 1 cut(s) 373
Csp6I GTAC 4 cut(s) 371, 551, 574, 613
CspAI ACCGGT 1 cut(s) 373
CviAII CATG 4 cut(s) 88, 142, 692, 829
CviQI GTAC 4 cut(s) 371, 551, 574, 613
DdeI CTNAG 2 cut(s) 65, 582
DpnI GATC 4 cut(s) 360, 609, 654, 690
DpnII GATC 4 cut(s) 358, 607, 652, 688
Eam1104I CTCTTC 2 cut(s) 720, 767
EarI CTCTTC 2 cut(s) 720, 767
Eco147I AGGCCT 1 cut(s) 328
Eco24I GRGCYC 1 cut(s) 675
Eco88I CYCGRG 1 cut(s) 249
EcoRI GAATTC 2 cut(s) 123, 194
EcoT38I GRGCYC 1 cut(s) 675
FaeI CATG 4 cut(s) 91, 145, 695, 832
FaiI YATR 5 cut(s) 89, 143, 558, 693, 830
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FaqI GGGAC 1 cut(s) 232
FatI CATG 4 cut(s) 87, 141, 691, 828
FauI CCCGC 3 cut(s) 154, 208, 662
FbaI TGATCA 1 cut(s) 688
Fnu4HI GCNGC 3 cut(s) 213, 297, 465
FriOI GRGCYC 1 cut(s) 675
Fsp4HI GCNGC 3 cut(s) 213, 297, 465
FspBI CTAG 2 cut(s) 300, 453
GluI GCNGC 3 cut(s) 213, 297, 465
HaeIII GGCC 1 cut(s) 328
HapII CCGG 1 cut(s) 374
Hin1II CATG 4 cut(s) 91, 145, 695, 832
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 643
HinfI GANTC 4 cut(s) 245, 268, 426, 474
HpaII CCGG 1 cut(s) 374
HphI GGTGA 4 cut(s) 17, 56, 428, 722
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 4 cut(s) 358, 363, 657, 677
Hpy188III TCNNGA 4 cut(s) 249, 430, 478, 692
Hpy8I GTNNAC 1 cut(s) 169
HpyAV CCTTC 6 cut(s) 92, 149, 614, 784, 794, 814
HpyCH4III ACNGT 2 cut(s) 44, 370
HpyCH4IV ACGT 1 cut(s) 750
HpyCH4V TGCA 5 cut(s) 22, 296, 320, 467, 758
HpyF10VI GCNNNNNNNGC 2 cut(s) 334, 679
HpyF3I CTNAG 2 cut(s) 65, 582
HpySE526I ACGT 1 cut(s) 750
Hsp92II CATG 4 cut(s) 91, 145, 695, 832
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 4 cut(s) 358, 607, 652, 688
LmnI GCTCC 2 cut(s) 158, 678
LpnPI CCDG 8 cut(s) 38, 42, 387, 456, 476, 533, 590, 696
Lsp1109I GCAGC 3 cut(s) 224, 308, 451
LweI GCATC 1 cut(s) 550
MaeI CTAG 2 cut(s) 300, 453
MaeII ACGT 1 cut(s) 750
MaeIII GTNAC 3 cut(s) 5, 455, 515
MalI GATC 4 cut(s) 360, 609, 654, 690
MbiI CCGCTC 1 cut(s) 161
MboI GATC 4 cut(s) 358, 607, 652, 688
MboII GAAGA 9 cut(s) 203, 266, 521, 734, 737, 746, 781, 784, 829
MfeI CAATTG 1 cut(s) 291
MhlI GDGCHC 1 cut(s) 675
MluCI AATT 6 cut(s) 70, 123, 194, 291, 346, 483
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 72
MroXI GAANNNNTTC 1 cut(s) 824
MseI TTAA 2 cut(s) 345, 633
MslI CAYNNNNRTG 2 cut(s) 45, 708
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 1 cut(s) 374
MunI CAATTG 1 cut(s) 291
MwoI GCNNNNNNNGC 2 cut(s) 334, 679
NdeII GATC 4 cut(s) 358, 607, 652, 688
NlaIII CATG 4 cut(s) 91, 145, 695, 832
NlaIV GGNNCC 2 cut(s) 378, 674
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 1 cut(s) 5
NspV TTCGAA 2 cut(s) 78, 132
PagI TCATGA 1 cut(s) 691
PceI AGGCCT 1 cut(s) 328
PdmI GAANNNNTTC 1 cut(s) 824
PfeI GAWTC 3 cut(s) 268, 426, 474
PinAI ACCGGT 1 cut(s) 373
PkrI GCNGC 3 cut(s) 214, 298, 466
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 378, 674
RsaI GTAC 4 cut(s) 372, 552, 575, 614
RsaNI GTAC 4 cut(s) 371, 551, 574, 613
RseI CAYNNNNRTG 2 cut(s) 45, 708
SaqAI TTAA 2 cut(s) 345, 633
SatI GCNGC 3 cut(s) 213, 297, 465
Sau3AI GATC 4 cut(s) 358, 607, 652, 688
SchI GAGTC 1 cut(s) 254
SduI GDGCHC 1 cut(s) 675
SetI ASST 8 cut(s) 301, 317, 442, 552, 594, 642, 647, 753
SfaNI GCATC 1 cut(s) 550
SfuI TTCGAA 2 cut(s) 78, 132
SmiMI CAYNNNNRTG 2 cut(s) 45, 708
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
Sse9I AATT 6 cut(s) 70, 123, 194, 291, 346, 483
SseBI AGGCCT 1 cut(s) 328
SsiI CCGC 3 cut(s) 161, 215, 669
SspMI CTAG 2 cut(s) 300, 453
StuI AGGCCT 1 cut(s) 328
TaaI ACNGT 2 cut(s) 44, 370
TaiI ACGT 1 cut(s) 753
TaqI TCGA 4 cut(s) 78, 132, 429, 477
TasI AATT 6 cut(s) 70, 123, 194, 291, 346, 483
TatI WGTACW 1 cut(s) 612
TfiI GAWTC 3 cut(s) 268, 426, 474
Tru1I TTAA 2 cut(s) 345, 633
Tru9I TTAA 2 cut(s) 345, 633
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 3 cut(s) 212, 296, 464
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 5 cut(s) 76, 168, 674, 803, 817
TspRI CASTG 1 cut(s) 39
XapI RAATTY 3 cut(s) 70, 123, 194
XmnI GAANNNNTTC 1 cut(s) 824
XspI CTAG 2 cut(s) 300, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.