Rw2G046860

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017350
Physical Location & Seq
Forward (+)
74274137 .. 74274973
837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G046860.1

Sequence Viewer

Length: 807 bp
ATGAGTGACGAGACAATCTTGCACCAGCCTGCAGCGAGCCACGGTGATGATGATGTTGAGAAACTGAGAAAATTTTTCGAAAAGTTCATGTTGGAGGAAGGGGTTGGTGTTGAGGAGTTGAGAATTCTTTTCGAAAAACTCATGTTCAGCAATGAAGGAGCGGAATTCATGTTCAAGGAAGCAGCGGGTGTTGAGGAGAAAAAGGAAGATGATGAGTCCCGAGAAGAAGCCGAGGAGAATCAACAAAAACAAGAGAAAACAATTGCAGCTAGATTGAAACAGAGGTCTGCATTAAGGCCTAAACTGCCGGTGCTTAATTTGAAATTGGATCGGAATACTGTACCGGTGCCAAAGCAATGGTGCGAGAAAGGGGTAGATTTGGAGGGTGAAAGTGGAATCGAGAAGCCACCTTATCAGCTTCCTAGTTACATTGCTGCAACTGGAATCGAGAAAATTAGACAGGCGTGGATTGAGAAAGAAGATAGTAACAAGTTGGGGGCAATGGATATTGATTATCAGGTACTTTATGATGCCTTTTTCAAGTACCAGACTAAGCCAAAGCTCACAGCACTTGGTGATCTGTACTATGAAGGAAAAGAGTTTAATCAGGTAAGCTTGAGGGATCAGATGAAAGACTGCGGGCTCCGAGAGCCAGTTGATCATGAGACAAAGCATTGGGGTGATTTGGAGGAAGAAGAGAGAGAAGATTTAGATGATGACATGGTTGCAGATAGTGAGAAGAAGAGGAAGCGTAAGATGAAGGAAAAGGAAGGCAAATACAACAAGAAGAAGGATTTCATGTTCTAA

Protein Analysis

268

Amino Acids

31.34

Weight (kDa)

4.98

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF382 PF04037 99 - 200 1.5e-30 Domain of unknown function (DUF382)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017350)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45270 FvH4_6g45280
rosa_chinensis RchiOBHm_Chr2g0163031
rosa_laevigata RLG00000021366
rosa_multiflora Rmu_sc0022169.1_g000001
rosa_roxburghii Rroxscaffold_2G00087610
rosa_rugosa Rorug02G0499900
rosa_samantha Rh2AG566200 Rh2BG578500 Rh2CG548400 Rh2DG588800 Rh2DG588900
rosa_wichuraiana Rw2G046860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AccBSI CCGCTC 1 cut(s) 161
AciI CCGC 3 cut(s) 161, 185, 639
AclWI GGATC 2 cut(s) 336, 630
AcsI RAATTY 3 cut(s) 71, 123, 164
AdeI CACNNNGTG 1 cut(s) 575
AfaI GTAC 4 cut(s) 342, 522, 545, 584
AgeI ACCGGT 1 cut(s) 343
AgsI TTSAA 4 cut(s) 175, 277, 322, 541
AloI GAACNNNNNNTCC 3 cut(s) 155, 187, 785
AluBI AGCT 4 cut(s) 269, 418, 562, 615
AluI AGCT 4 cut(s) 269, 418, 562, 615
Alw26I GTCTC 2 cut(s) 5, 659
AlwI GGATC 2 cut(s) 336, 630
Ama87I CYCGRG 1 cut(s) 219
AoxI GGCC 1 cut(s) 296
ApeKI GCWGC 4 cut(s) 32, 182, 266, 434
ApoI RAATTY 3 cut(s) 71, 123, 164
AsiGI ACCGGT 1 cut(s) 343
Asp700I GAANNNNTTC 1 cut(s) 794
AsuHPI GGTGA 4 cut(s) 56, 398, 587, 692
AsuII TTCGAA 2 cut(s) 78, 132
AvaI CYCGRG 1 cut(s) 219
BanI GGYRCC 1 cut(s) 346
BanII GRGCYC 1 cut(s) 645
BbvI GCAGC 4 cut(s) 44, 194, 278, 421
BclI TGATCA 1 cut(s) 658
BcoDI GTCTC 2 cut(s) 5, 659
BfaI CTAG 2 cut(s) 270, 423
BfmI CTRYAG 1 cut(s) 30
BisI GCNGC 4 cut(s) 33, 183, 267, 435
BlsI GCNGC 4 cut(s) 34, 184, 268, 436
BmeT110I CYCGRG 1 cut(s) 219
BmiI GGNNCC 2 cut(s) 348, 644
BmsI GCATC 1 cut(s) 520
Bpu14I TTCGAA 2 cut(s) 78, 132
BpuEI CTTGAG 1 cut(s) 637
BsaJI CCNNGG 2 cut(s) 40, 231
BsaWI WCCGGW 1 cut(s) 343
Bse118I RCCGGY 2 cut(s) 307, 343
Bse1I ACTGG 2 cut(s) 445, 653
Bse3DI GCAATG 4 cut(s) 157, 362, 429, 507
BseDI CCNNGG 2 cut(s) 40, 231
BseMI GCAATG 4 cut(s) 157, 362, 429, 507
BseMII CTCAG 1 cut(s) 56
BseNI ACTGG 2 cut(s) 445, 653
BseRI GAGGAG 3 cut(s) 128, 209, 248
BseXI GCAGC 4 cut(s) 44, 194, 278, 421
BshFI GGCC 1 cut(s) 298
BshNI GGYRCC 1 cut(s) 346
BshTI ACCGGT 1 cut(s) 343
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 2 cut(s) 308, 344
BslFI GGGAC 1 cut(s) 202
BsmAI GTCTC 2 cut(s) 5, 659
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 1 cut(s) 298
BsoBI CYCGRG 1 cut(s) 219
Bsp119I TTCGAA 2 cut(s) 78, 132
Bsp1286I GDGCHC 1 cut(s) 645
Bsp143I GATC 4 cut(s) 328, 577, 622, 658
BspACI CCGC 3 cut(s) 161, 185, 639
BspANI GGCC 1 cut(s) 298
BspCNI CTCAG 1 cut(s) 57
BspHI TCATGA 1 cut(s) 661
BspLI GGNNCC 2 cut(s) 348, 644
BspMAI CTGCAG 1 cut(s) 34
BspPI GGATC 2 cut(s) 336, 630
BspT104I TTCGAA 2 cut(s) 78, 132
BspT107I GGYRCC 1 cut(s) 346
BsrBI CCGCTC 1 cut(s) 161
BsrDI GCAATG 4 cut(s) 157, 362, 429, 507
BsrFI RCCGGY 2 cut(s) 307, 343
BsrI ACTGG 2 cut(s) 445, 653
BssAI RCCGGY 2 cut(s) 307, 343
BssECI CCNNGG 2 cut(s) 40, 231
BssMI GATC 4 cut(s) 328, 577, 622, 658
Bst4CI ACNGT 2 cut(s) 44, 340
Bst6I CTCTTC 2 cut(s) 690, 737
BstBI TTCGAA 2 cut(s) 78, 132
BstC8I GCNNGC 3 cut(s) 30, 37, 641
BstDEI CTNAG 2 cut(s) 65, 552
BstDSI CCRYGG 1 cut(s) 40
BstKTI GATC 4 cut(s) 331, 580, 625, 661
BstMAI GTCTC 2 cut(s) 5, 659
BstMBI GATC 4 cut(s) 328, 577, 622, 658
BstMWI GCNNNNNNNGC 2 cut(s) 304, 649
BstSFI CTRYAG 1 cut(s) 30
BstV1I GCAGC 4 cut(s) 44, 194, 278, 421
BstXI CCANNNNNNTGG 1 cut(s) 357
BsuRI GGCC 1 cut(s) 298
BtgI CCRYGG 1 cut(s) 40
Cac8I GCNNGC 3 cut(s) 30, 37, 641
CciI TCATGA 1 cut(s) 661
Cfr10I RCCGGY 2 cut(s) 307, 343
Csp6I GTAC 4 cut(s) 341, 521, 544, 583
CspAI ACCGGT 1 cut(s) 343
CviAII CATG 6 cut(s) 88, 142, 169, 662, 721, 799
CviQI GTAC 4 cut(s) 341, 521, 544, 583
DdeI CTNAG 2 cut(s) 65, 552
DpnI GATC 4 cut(s) 330, 579, 624, 660
DpnII GATC 4 cut(s) 328, 577, 622, 658
DraIII CACNNNGTG 1 cut(s) 575
Eam1104I CTCTTC 2 cut(s) 690, 737
EarI CTCTTC 2 cut(s) 690, 737
Eco147I AGGCCT 1 cut(s) 298
Eco24I GRGCYC 1 cut(s) 645
Eco88I CYCGRG 1 cut(s) 219
EcoRI GAATTC 2 cut(s) 123, 164
EcoT38I GRGCYC 1 cut(s) 645
FaeI CATG 6 cut(s) 91, 145, 172, 665, 724, 802
FaiI YATR 8 cut(s) 89, 143, 170, 528, 588, 663, 722, 800
FaqI GGGAC 1 cut(s) 202
FatI CATG 6 cut(s) 87, 141, 168, 661, 720, 798
FauI CCCGC 2 cut(s) 178, 632
FbaI TGATCA 1 cut(s) 658
Fnu4HI GCNGC 4 cut(s) 33, 183, 267, 435
FriOI GRGCYC 1 cut(s) 645
Fsp4HI GCNGC 4 cut(s) 33, 183, 267, 435
FspBI CTAG 2 cut(s) 270, 423
GluI GCNGC 4 cut(s) 33, 183, 267, 435
HaeIII GGCC 1 cut(s) 298
HapII CCGG 2 cut(s) 308, 344
Hin1II CATG 6 cut(s) 91, 145, 172, 665, 724, 802
HindIII AAGCTT 1 cut(s) 613
HinfI GANTC 4 cut(s) 215, 238, 396, 444
HpaII CCGG 2 cut(s) 308, 344
HphI GGTGA 4 cut(s) 56, 398, 587, 692
Hpy188I TCNGA 3 cut(s) 333, 627, 647
Hpy188III TCNNGA 4 cut(s) 219, 400, 448, 662
HpyAV CCTTC 6 cut(s) 92, 149, 584, 754, 764, 784
HpyCH4III ACNGT 2 cut(s) 44, 340
HpyCH4V TGCA 6 cut(s) 22, 32, 266, 290, 437, 728
HpyF10VI GCNNNNNNNGC 2 cut(s) 304, 649
HpyF3I CTNAG 2 cut(s) 65, 552
Hsp92II CATG 6 cut(s) 91, 145, 172, 665, 724, 802
Ksp22I TGATCA 1 cut(s) 658
Kzo9I GATC 4 cut(s) 328, 577, 622, 658
LmnI GCTCC 2 cut(s) 158, 648
Lsp1109I GCAGC 4 cut(s) 44, 194, 278, 421
LweI GCATC 1 cut(s) 520
MaeI CTAG 2 cut(s) 270, 423
MaeIII GTNAC 3 cut(s) 5, 425, 485
MalI GATC 4 cut(s) 330, 579, 624, 660
MbiI CCGCTC 1 cut(s) 161
MboI GATC 4 cut(s) 328, 577, 622, 658
MboII GAAGA 9 cut(s) 218, 236, 491, 704, 707, 716, 751, 754, 799
MfeI CAATTG 1 cut(s) 261
MhlI GDGCHC 1 cut(s) 645
MluCI AATT 7 cut(s) 71, 123, 164, 261, 316, 323, 453
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 9 cut(s) 88, 106, 187, 226, 276, 376, 612, 682, 738
MroXI GAANNNNTTC 1 cut(s) 794
MseI TTAA 3 cut(s) 293, 315, 603
MslI CAYNNNNRTG 2 cut(s) 45, 678
MspA1I CMGCKG 1 cut(s) 185
MspI CCGG 2 cut(s) 308, 344
MunI CAATTG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 304, 649
NdeII GATC 4 cut(s) 328, 577, 622, 658
NlaIII CATG 6 cut(s) 91, 145, 172, 665, 724, 802
NlaIV GGNNCC 2 cut(s) 348, 644
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 5
NspV TTCGAA 2 cut(s) 78, 132
PagI TCATGA 1 cut(s) 661
PceI AGGCCT 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 794
PfeI GAWTC 3 cut(s) 238, 396, 444
PinAI ACCGGT 1 cut(s) 343
PkrI GCNGC 4 cut(s) 34, 184, 268, 436
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
PspN4I GGNNCC 2 cut(s) 348, 644
PstI CTGCAG 1 cut(s) 34
RsaI GTAC 4 cut(s) 342, 522, 545, 584
RsaNI GTAC 4 cut(s) 341, 521, 544, 583
RseI CAYNNNNRTG 2 cut(s) 45, 678
SaqAI TTAA 3 cut(s) 293, 315, 603
SatI GCNGC 4 cut(s) 33, 183, 267, 435
Sau3AI GATC 4 cut(s) 328, 577, 622, 658
SchI GAGTC 1 cut(s) 224
SduI GDGCHC 1 cut(s) 645
SetI ASST 8 cut(s) 271, 287, 412, 420, 522, 564, 612, 617
SfaNI GCATC 1 cut(s) 520
SfcI CTRYAG 1 cut(s) 30
SfuI TTCGAA 2 cut(s) 78, 132
SmiMI CAYNNNNRTG 2 cut(s) 45, 678
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
Sse9I AATT 7 cut(s) 71, 123, 164, 261, 316, 323, 453
SseBI AGGCCT 1 cut(s) 298
SsiI CCGC 3 cut(s) 161, 185, 639
SspMI CTAG 2 cut(s) 270, 423
StuI AGGCCT 1 cut(s) 298
TaaI ACNGT 2 cut(s) 44, 340
TaqI TCGA 4 cut(s) 78, 132, 399, 447
TasI AATT 7 cut(s) 71, 123, 164, 261, 316, 323, 453
TatI WGTACW 1 cut(s) 582
TfiI GAWTC 3 cut(s) 238, 396, 444
Tru1I TTAA 3 cut(s) 293, 315, 603
Tru9I TTAA 3 cut(s) 293, 315, 603
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 4 cut(s) 32, 182, 266, 434
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 7 cut(s) 76, 157, 168, 603, 644, 773, 787
XapI RAATTY 3 cut(s) 71, 123, 164
XmnI GAANNNNTTC 1 cut(s) 794
XspI CTAG 2 cut(s) 270, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.