RchiOBHm_Chr1g0323441

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0017412
Physical Location & Seq
Reverse (-)
10918913 .. 10920108
1196 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
ATGCTTAATATGAGCTTGTTAGTTTGTAGAGCCATTGATGAAAACAGAAAGGTCCATATAGTATACATGGGCTCACTTCCCGCTAACCATTCGTATTCACCATTGTCTCACCACCTTAATATGTTAGAAAGAGTTGTCAAGAATGGTTCAAATCTCCTGACGAGAAGTTACAAAAGGAGTTTCAATGGATTTGCTGCCAAGCTCACTGACAATGAAAGACATGTGCTTGCTAACATGAAGGAAGTAGAATTAGACAGCTTCAAAGATGACGGATTTGGTCCGGCTCCAAAGAAATGGAAAGGTGCGTGTGCAGGTGGCAGAAACCTCACATGCAACAACAAGATCATTGGAGCTCGGTTTTACCAACCAATACAATCTGCAAGGGATGAAGAAGGCCATGGAACCCACACTGCCTCAACGGCAGCAGGAAATGCCGTAAAGGATGTGAGCTTTTATAGACTAGCACAAGGATCAGAACAGCAAGAAGAAACAGTGGTCCTAGAGCTGGTTCTGTTACAAGCGTGGCACCATGGTTGCTCTCAGTGGCAGGCGGCAGCTAGTAGCACTGATCGTCGAATCATTGACACTGTCGTTCTTGGAAATGGAACCACAATTGTTGGGTTATCAGTGAATTCTTTCACATTAAATGGAACAAGTTTCCCATTGATATATGGAAAAGCTGCGTCAATGAAATGCTCGGAGTTGGGTGCGGGGCAATGTCACTCAGGCTGCCTGGATAGTGATTTTGTGAAGGGAAAGATTGTTTTGTGTGATGACTACTTCGAAGGTGATGTAGCTTACCAAGCTAAAGCAGTAGGTTCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

275

Amino Acids

29.94

Weight (kDa)

7.63

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 18 - 83 2.2e-10 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 302
AccB1I GGYRCC 1 cut(s) 525
AccI GTMKAC 1 cut(s) 63
AciI CCGC 3 cut(s) 81, 551, 710
AclWI GGATC 1 cut(s) 478
AcsI RAATTY 1 cut(s) 631
AfiI CCNNNNNNNGG 1 cut(s) 505
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 3 cut(s) 150, 184, 262
AjnI CCWGG 1 cut(s) 732
Alw21I GWGCWC 1 cut(s) 355
Alw26I GTCTC 1 cut(s) 111
AlwI GGATC 1 cut(s) 478
AoxI GGCC 1 cut(s) 394
ApeKI GCWGC 5 cut(s) 194, 422, 554, 680, 729
ApoI RAATTY 1 cut(s) 631
Asp700I GAANNNNTTC 1 cut(s) 635
AspS9I GGNCC 3 cut(s) 52, 278, 496
AsuHPI GGTGA 3 cut(s) 90, 101, 800
AsuII TTCGAA 1 cut(s) 783
AvaII GGWCC 3 cut(s) 52, 278, 496
BanI GGYRCC 1 cut(s) 525
BanII GRGCYC 2 cut(s) 74, 355
Bbv12I GWGCWC 1 cut(s) 355
BbvI GCAGC 5 cut(s) 181, 434, 566, 667, 716
BceAI ACGGC 2 cut(s) 419, 435
BciT130I CCWGG 1 cut(s) 734
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 3 cut(s) 461, 500, 558
BfuAI ACCTGC 1 cut(s) 302
BglI GCCNNNNNGGC 1 cut(s) 419
BisI GCNGC 6 cut(s) 195, 423, 552, 555, 681, 730
BlsI GCNGC 6 cut(s) 196, 424, 553, 556, 682, 731
Bme1390I CCNGG 1 cut(s) 734
Bme18I GGWCC 3 cut(s) 52, 278, 496
BmgT120I GGNCC 3 cut(s) 52, 278, 496
BmiI GGNNCC 4 cut(s) 285, 403, 527, 607
BmrFI CCNGG 1 cut(s) 734
Bpu14I TTCGAA 1 cut(s) 783
BsaJI CCNNGG 2 cut(s) 397, 529
Bsc4I CCNNNNNNNGG 1 cut(s) 505
Bse3DI GCAATG 1 cut(s) 722
BseBI CCWGG 1 cut(s) 734
BseDI CCNNGG 2 cut(s) 397, 529
BseGI GGATG 2 cut(s) 391, 448
BseLI CCNNNNNNNGG 1 cut(s) 505
BseMI GCAATG 1 cut(s) 722
BseMII CTCAG 2 cut(s) 554, 738
BseXI GCAGC 5 cut(s) 181, 434, 566, 667, 716
BsgI GTGCAG 1 cut(s) 330
BshFI GGCC 1 cut(s) 396
BshNI GGYRCC 1 cut(s) 525
BsiHKAI GWGCWC 1 cut(s) 355
BsiSI CCGG 1 cut(s) 281
BslI CCNNNNNNNGG 1 cut(s) 505
BsmAI GTCTC 1 cut(s) 111
BsnI GGCC 1 cut(s) 396
Bsp119I TTCGAA 1 cut(s) 783
Bsp1286I GDGCHC 2 cut(s) 74, 355
Bsp143I GATC 3 cut(s) 342, 470, 568
Bsp19I CCATGG 2 cut(s) 397, 529
BspACI CCGC 3 cut(s) 81, 551, 710
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 2 cut(s) 553, 737
BspLI GGNNCC 4 cut(s) 285, 403, 527, 607
BspMI ACCTGC 1 cut(s) 302
BspPI GGATC 1 cut(s) 478
BspT104I TTCGAA 1 cut(s) 783
BspT107I GGYRCC 1 cut(s) 525
BsrDI GCAATG 1 cut(s) 722
BssECI CCNNGG 2 cut(s) 397, 529
BssMI GATC 3 cut(s) 342, 470, 568
BssNAI GTATAC 1 cut(s) 64
BssT1I CCWWGG 2 cut(s) 397, 529
Bst1107I GTATAC 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 734
Bst4CI ACNGT 2 cut(s) 493, 589
BstAPI GCANNNNNTGC 1 cut(s) 431
BstBI TTCGAA 1 cut(s) 783
BstC8I GCNNGC 2 cut(s) 228, 549
BstDEI CTNAG 2 cut(s) 540, 724
BstDSI CCRYGG 2 cut(s) 397, 529
BstF5I GGATG 2 cut(s) 391, 448
BstKTI GATC 3 cut(s) 345, 473, 571
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 3 cut(s) 342, 470, 568
BstMWI GCNNNNNNNGC 3 cut(s) 419, 431, 803
BstNI CCWGG 1 cut(s) 734
BstNSI RCATGY 2 cut(s) 224, 333
BstSCI CCNGG 1 cut(s) 732
BstV1I GCAGC 5 cut(s) 181, 434, 566, 667, 716
BstXI CCANNNNNNTGG 1 cut(s) 294
BstZ17I GTATAC 1 cut(s) 64
BsuRI GGCC 1 cut(s) 396
BtgI CCRYGG 2 cut(s) 397, 529
BtsCI GGATG 2 cut(s) 391, 448
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 7 cut(s) 204, 408, 498, 548, 564, 585, 633
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 2 cut(s) 228, 549
Cfr13I GGNCC 3 cut(s) 52, 278, 496
CseI GACGC 1 cut(s) 672
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviAII CATG 6 cut(s) 67, 221, 235, 330, 398, 530
DdeI CTNAG 2 cut(s) 540, 724
DpnI GATC 3 cut(s) 344, 472, 570
DpnII GATC 3 cut(s) 342, 470, 568
Ecl136II GAGCTC 1 cut(s) 353
Eco130I CCWWGG 2 cut(s) 397, 529
Eco24I GRGCYC 2 cut(s) 74, 355
Eco47I GGWCC 3 cut(s) 52, 278, 496
Eco53kI GAGCTC 1 cut(s) 353
EcoICRI GAGCTC 1 cut(s) 353
EcoRI GAATTC 1 cut(s) 631
EcoRII CCWGG 1 cut(s) 732
EcoT14I CCWWGG 2 cut(s) 397, 529
EcoT38I GRGCYC 2 cut(s) 74, 355
ErhI CCWWGG 2 cut(s) 397, 529
FaeI CATG 6 cut(s) 70, 224, 238, 333, 401, 533
FatI CATG 6 cut(s) 66, 220, 234, 329, 397, 529
FauI CCCGC 2 cut(s) 88, 703
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 6 cut(s) 195, 423, 552, 555, 681, 730
FokI GGATG 2 cut(s) 398, 455
FriOI GRGCYC 2 cut(s) 74, 355
Fsp4HI GCNGC 6 cut(s) 195, 423, 552, 555, 681, 730
FspBI CTAG 3 cut(s) 461, 500, 558
GluI GCNGC 6 cut(s) 195, 423, 552, 555, 681, 730
HaeIII GGCC 1 cut(s) 396
HapII CCGG 1 cut(s) 281
HgaI GACGC 1 cut(s) 672
Hin1II CATG 6 cut(s) 70, 224, 238, 333, 401, 533
HinfI GANTC 1 cut(s) 576
HpaII CCGG 1 cut(s) 281
HphI GGTGA 3 cut(s) 90, 101, 800
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 2 cut(s) 475, 700
Hpy188III TCNNGA 2 cut(s) 139, 157
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 1 cut(s) 576
HpyAV CCTTC 4 cut(s) 232, 386, 745, 779
HpyCH4III ACNGT 2 cut(s) 493, 589
HpyCH4V TGCA 3 cut(s) 311, 333, 380
HpyF10VI GCNNNNNNNGC 3 cut(s) 419, 431, 803
HpyF3I CTNAG 2 cut(s) 540, 724
Hsp92II CATG 6 cut(s) 70, 224, 238, 333, 401, 533
Kzo9I GATC 3 cut(s) 342, 470, 568
LmnI GCTCC 2 cut(s) 289, 350
LpnPI CCDG 9 cut(s) 170, 294, 297, 411, 491, 533, 711, 719, 746
Lsp1109I GCAGC 5 cut(s) 181, 434, 566, 667, 716
MaeI CTAG 3 cut(s) 461, 500, 558
MaeIII GTNAC 3 cut(s) 167, 513, 719
MalI GATC 3 cut(s) 344, 472, 570
MboI GATC 3 cut(s) 342, 470, 568
MboII GAAGA 2 cut(s) 401, 497
MfeI CAATTG 1 cut(s) 612
MhlI GDGCHC 2 cut(s) 74, 355
MluCI AATT 3 cut(s) 248, 612, 631
MnlI CCTC 2 cut(s) 335, 424
MroXI GAANNNNTTC 1 cut(s) 635
MseI TTAA 4 cut(s) 6, 117, 644, 826
MspI CCGG 1 cut(s) 281
MspR9I CCNGG 1 cut(s) 734
MunI CAATTG 1 cut(s) 612
MvaI CCWGG 1 cut(s) 734
MwoI GCNNNNNNNGC 3 cut(s) 419, 431, 803
NcoI CCATGG 2 cut(s) 397, 529
NdeII GATC 3 cut(s) 342, 470, 568
NlaIII CATG 6 cut(s) 70, 224, 238, 333, 401, 533
NlaIV GGNNCC 4 cut(s) 285, 403, 527, 607
NmuCI GTSAC 1 cut(s) 719
NspI RCATGY 2 cut(s) 224, 333
NspV TTCGAA 1 cut(s) 783
PaqCI CACCTGC 1 cut(s) 302
PciI ACATGT 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 635
PfeI GAWTC 1 cut(s) 576
PflFI GACNNNGTC 1 cut(s) 587
PkrI GCNGC 6 cut(s) 196, 424, 553, 556, 682, 731
PscI ACATGT 1 cut(s) 220
Psp124BI GAGCTC 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 732
PspGI CCWGG 1 cut(s) 732
PspN4I GGNNCC 4 cut(s) 285, 403, 527, 607
PspPI GGNCC 3 cut(s) 52, 278, 496
PsyI GACNNNGTC 1 cut(s) 587
SacI GAGCTC 1 cut(s) 355
SaqAI TTAA 4 cut(s) 6, 117, 644, 826
SatI GCNGC 6 cut(s) 195, 423, 552, 555, 681, 730
Sau3AI GATC 3 cut(s) 342, 470, 568
Sau96I GGNCC 3 cut(s) 52, 278, 496
ScrFI CCNGG 1 cut(s) 734
SduI GDGCHC 2 cut(s) 74, 355
SfuI TTCGAA 1 cut(s) 783
SinI GGWCC 3 cut(s) 52, 278, 496
Sse9I AATT 3 cut(s) 248, 612, 631
SsiI CCGC 3 cut(s) 81, 551, 710
SspMI CTAG 3 cut(s) 461, 500, 558
SstI GAGCTC 1 cut(s) 355
StyD4I CCNGG 1 cut(s) 732
StyI CCWWGG 2 cut(s) 397, 529
TaaI ACNGT 2 cut(s) 493, 589
TaqI TCGA 2 cut(s) 574, 783
TasI AATT 3 cut(s) 248, 612, 631
TauI GCSGC 1 cut(s) 554
TfiI GAWTC 1 cut(s) 576
Tru1I TTAA 4 cut(s) 6, 117, 644, 826
Tru9I TTAA 4 cut(s) 6, 117, 644, 826
TscAI CASTG 7 cut(s) 211, 415, 498, 548, 571, 592, 633
TseFI GTSAC 1 cut(s) 719
TseI GCWGC 5 cut(s) 194, 422, 554, 680, 729
Tsp45I GTSAC 1 cut(s) 719
TspDTI ATGAA 6 cut(s) 54, 228, 251, 402, 704, 810
TspGWI ACGGA 1 cut(s) 285
TspRI CASTG 7 cut(s) 211, 415, 498, 548, 571, 592, 633
Tth111I GACNNNGTC 1 cut(s) 587
VpaK11BI GGWCC 3 cut(s) 52, 278, 496
XapI RAATTY 1 cut(s) 631
XceI RCATGY 2 cut(s) 224, 333
XmiI GTMKAC 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 3 cut(s) 461, 500, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.