RLG00000014858

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017412
Physical Location & Seq
Reverse (-)
58624691 .. 58626350
1660 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014858

Sequence Viewer

Length: 1110 bp
ATGAAAACAGAAAGGTCCATAGTTTACCTCGTCTCACTTCCAGATAATAATTTGTACTTACCATTGTCTCACCACCTTTGCATACTAGAAAGAGTTGTCAAGAATGGCTCGGGTGCAAATCCGTTGTCAAGAAGTTACAAAAGGAGTTTCAATGGATTTGCTGCGAAGCTCACTGACCTTGAAAGACAAAAGATTGATAACAGGAACGAAGTGGTCTCTGTCTTTCTAAGCACAACTTTCCGACTTCAAACAACAAGATTGTGGGACTTCAACGGTCAAAATGAGAAAATCCATCGAAATGCCACAGTAGAGTTTCAGTCCGGCTCCAAGCAGTGGAAAGGTGCTTGTGCAGGTGGAAAAAACTTCACTTGCAACAACAAGATCAGCAGAGCTCGGCCATGGAACCCACACTGCCTCAACGGCAGCAGGGAATGCCGTAAAGGACTAGTACAAGGTACTACAAGAGGTGGAGTTCCCTTTGCGAGGTTTGCTACATATAAAGTCTGCAATGGCGAATGGTGCCGTTCAGAGGGTATCCTGAGTGCTTTTGACGATGCTATTGCTGATGGAGTTGACATCATTACTGTTTCACTTACTCACATACTTACAGTACGTTTGAATAAAGATCCGATAGCAATAGTACTAACAACTAAAACTGCAGGCAACGATGGTTATAGAGCTGGTTCTGTAACAAGTGTGGCACCATGTTCTCCATTAGTATATGTGAAATCTGCTTCAATGAAGGGAAAGGTCGTCTTATCTGATGGTTACATTGGAGGTGATGTGGCTTATGAAGCTGGAGCAAATGTTACTGATGATGCCCCAGGTGAATTTGCTTATGGATCTGGACATATCAGTCCTGTCCAAGCAATAGATCCAGGGCTGGTTTATGAAGCTTCTAAGGAAGGTTACATTAAGTTGCTCTGCTTGGTCTTGGGAGAGGCCGAGAATTTCACAAACTCCACTCGAGTGGACATCAAGGTTGTGCCTGAAGCTTTTTCTTTCAAGTCTTTGAATGAGGAGGAGAAGCCTTTTGATGTGACCGTTGTTGGAGGAGGATTGTCAGATGGAACACAACCGACATGTGTCGGCATTGCTGGTTTGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

370

Amino Acids

39.91

Weight (kDa)

8.44

Isoelectric Point (pI)

28.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 23 - 82 1.5e-09 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 341
AasI GACNNNNNNGTC 1 cut(s) 855
Acc36I ACCTGC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 519, 700
AccB7I CCANNNNNTGG 1 cut(s) 333
AclWI GGATC 3 cut(s) 620, 850, 869
AcoI YGGCCR 1 cut(s) 395
AcsI RAATTY 2 cut(s) 830, 949
AcuI CTGAAG 1 cut(s) 1011
AfaI GTAC 5 cut(s) 56, 450, 457, 612, 642
AfiI CCNNNNNNNGG 3 cut(s) 333, 483, 529
AflIII ACRYGT 1 cut(s) 1082
AgsI TTSAA 8 cut(s) 151, 182, 248, 271, 619, 738, 1006, 1015
AhlI ACTAGT 1 cut(s) 445
AjnI CCWGG 2 cut(s) 823, 877
AleI CACNNNNGTG 1 cut(s) 968
AluBI AGCT 6 cut(s) 169, 392, 680, 797, 896, 995
AluI AGCT 6 cut(s) 169, 392, 680, 797, 896, 995
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 3 cut(s) 37, 72, 220
AlwI GGATC 3 cut(s) 620, 850, 869
Ama87I CYCGRG 2 cut(s) 109, 966
AoxI GGCC 2 cut(s) 395, 942
ApeKI GCWGC 2 cut(s) 161, 423
ApoI RAATTY 2 cut(s) 830, 949
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
AspS9I GGNCC 1 cut(s) 15
AsuHPI GGTGA 3 cut(s) 62, 791, 839
AvaI CYCGRG 2 cut(s) 109, 966
AvaII GGWCC 1 cut(s) 15
BanI GGYRCC 2 cut(s) 519, 700
BanII GRGCYC 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 394
BbvI GCAGC 2 cut(s) 148, 435
BccI CCATC 5 cut(s) 300, 560, 662, 758, 1061
BceAI ACGGC 3 cut(s) 420, 436, 507
BcgI CGANNNNNNTGC 2 cut(s) 542, 576
BciT130I CCWGG 2 cut(s) 825, 879
BciVI GTATCC 1 cut(s) 545
BcoDI GTCTC 3 cut(s) 37, 72, 220
BcuI ACTAGT 1 cut(s) 445
BfaI CTAG 2 cut(s) 86, 446
BfmI CTRYAG 1 cut(s) 657
BfuAI ACCTGC 1 cut(s) 341
BfuI GTATCC 1 cut(s) 545
BglI GCCNNNNNGGC 1 cut(s) 420
BisI GCNGC 2 cut(s) 162, 424
BlsI GCNGC 2 cut(s) 163, 425
BmcAI AGTACT 1 cut(s) 642
Bme1390I CCNGG 2 cut(s) 825, 879
Bme18I GGWCC 1 cut(s) 15
BmeT110I CYCGRG 2 cut(s) 109, 966
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 4 cut(s) 325, 404, 521, 702
BmrFI CCNGG 2 cut(s) 825, 879
BmsI GCATC 2 cut(s) 544, 808
BoxI GACNNNNGTC 1 cut(s) 1085
BpmI CTGGAG 1 cut(s) 819
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 3 cut(s) 398, 823, 878
BsaXI ACNNNNNCTCC 4 cut(s) 768, 792, 798, 822
Bsc4I CCNNNNNNNGG 3 cut(s) 333, 483, 529
Bse3DI GCAATG 2 cut(s) 514, 1092
BseBI CCWGG 2 cut(s) 825, 879
BseDI CCNNGG 3 cut(s) 398, 823, 878
BseLI CCNNNNNNNGG 3 cut(s) 333, 483, 529
BseMI GCAATG 2 cut(s) 514, 1092
BseMII CTCAG 1 cut(s) 530
BseRI GAGGAG 3 cut(s) 1034, 1037, 1068
BseXI GCAGC 2 cut(s) 148, 435
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 2 cut(s) 397, 944
BshNI GGYRCC 2 cut(s) 519, 700
BsiHKAI GWGCWC 1 cut(s) 394
BsiHKCI CYCGRG 2 cut(s) 109, 966
BsiSI CCGG 1 cut(s) 321
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 3 cut(s) 333, 483, 529
BsmAI GTCTC 3 cut(s) 37, 72, 220
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 2 cut(s) 397, 944
Bso31I GGTCTC 1 cut(s) 220
BsoBI CYCGRG 2 cut(s) 109, 966
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 4 cut(s) 381, 625, 842, 874
Bsp19I CCATGG 1 cut(s) 398
BspANI GGCC 2 cut(s) 397, 944
BspCNI CTCAG 1 cut(s) 531
BspLI GGNNCC 4 cut(s) 325, 404, 521, 702
BspMAI CTGCAG 1 cut(s) 661
BspMI ACCTGC 1 cut(s) 341
BspPI GGATC 3 cut(s) 620, 850, 869
BspT107I GGYRCC 2 cut(s) 519, 700
BspTNI GGTCTC 1 cut(s) 220
BsrDI GCAATG 2 cut(s) 514, 1092
BssECI CCNNGG 3 cut(s) 398, 823, 878
BssMI GATC 4 cut(s) 381, 625, 842, 874
BssT1I CCWWGG 1 cut(s) 398
Bst2UI CCWGG 2 cut(s) 825, 879
Bst4CI ACNGT 5 cut(s) 275, 307, 586, 610, 1045
BstAPI GCANNNNNTGC 1 cut(s) 432
BstC8I GCNNGC 1 cut(s) 661
BstDEI CTNAG 3 cut(s) 227, 539, 900
BstDSI CCRYGG 1 cut(s) 398
BstENI CCTNNNNNAGG 1 cut(s) 481
BstKTI GATC 4 cut(s) 384, 628, 845, 877
BstMAI GTCTC 3 cut(s) 37, 72, 220
BstMBI GATC 4 cut(s) 381, 625, 842, 874
BstMWI GCNNNNNNNGC 5 cut(s) 420, 432, 488, 519, 794
BstNI CCWGG 2 cut(s) 825, 879
BstNSI RCATGY 1 cut(s) 1086
BstPAI GACNNNNGTC 1 cut(s) 1085
BstSCI CCNGG 2 cut(s) 823, 877
BstSFI CTRYAG 1 cut(s) 657
BstV1I GCAGC 2 cut(s) 148, 435
BstX2I RGATCY 3 cut(s) 625, 842, 874
BstXI CCANNNNNNTGG 1 cut(s) 970
BstYI RGATCY 3 cut(s) 625, 842, 874
BsuI GTATCC 1 cut(s) 545
BsuRI GGCC 2 cut(s) 397, 944
BtgI CCRYGG 1 cut(s) 398
BtsI GCAGTG 2 cut(s) 338, 409
BtsIMutI CAGTG 3 cut(s) 171, 338, 409
BveI ACCTGC 1 cut(s) 341
Cac8I GCNNGC 1 cut(s) 661
Cfr13I GGNCC 1 cut(s) 15
Csp6I GTAC 5 cut(s) 55, 449, 456, 611, 641
CviAII CATG 3 cut(s) 399, 705, 1083
CviQI GTAC 5 cut(s) 55, 449, 456, 611, 641
DdeI CTNAG 3 cut(s) 227, 539, 900
DpnI GATC 4 cut(s) 383, 627, 844, 876
DpnII GATC 4 cut(s) 381, 625, 842, 874
DrdI GACNNNNNNGTC 1 cut(s) 855
DseDI GACNNNNNNGTC 1 cut(s) 855
EaeI YGGCCR 1 cut(s) 395
Ecl136II GAGCTC 1 cut(s) 392
Eco130I CCWWGG 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 394
Eco31I GGTCTC 1 cut(s) 220
Eco47I GGWCC 1 cut(s) 15
Eco53kI GAGCTC 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 1011
Eco88I CYCGRG 2 cut(s) 109, 966
EcoICRI GAGCTC 1 cut(s) 392
EcoNI CCTNNNNNAGG 1 cut(s) 481
EcoRII CCWGG 2 cut(s) 823, 877
EcoT14I CCWWGG 1 cut(s) 398
EcoT38I GRGCYC 1 cut(s) 394
ErhI CCWWGG 1 cut(s) 398
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 3 cut(s) 402, 708, 1086
FalI AAGNNNNNCTT 4 cut(s) 220, 252, 740, 772
FaqI GGGAC 1 cut(s) 278
FatI CATG 3 cut(s) 398, 704, 1082
Fnu4HI GCNGC 2 cut(s) 162, 424
FriOI GRGCYC 1 cut(s) 394
Fsp4HI GCNGC 2 cut(s) 162, 424
FspBI CTAG 2 cut(s) 86, 446
GluI GCNGC 2 cut(s) 162, 424
GsuI CTGGAG 1 cut(s) 819
HaeIII GGCC 2 cut(s) 397, 944
HapII CCGG 1 cut(s) 321
Hin1II CATG 3 cut(s) 402, 708, 1086
HincII GTYRAC 1 cut(s) 574
HindII GTYRAC 1 cut(s) 574
HindIII AAGCTT 2 cut(s) 894, 993
HpaII CCGG 1 cut(s) 321
HphI GGTGA 3 cut(s) 62, 791, 839
Hpy166II GTNNAC 3 cut(s) 25, 574, 973
Hpy188I TCNGA 6 cut(s) 242, 529, 630, 763, 1066, 1109
Hpy188III TCNNGA 5 cut(s) 41, 100, 129, 538, 846
Hpy8I GTNNAC 3 cut(s) 25, 574, 973
HpyAV CCTTC 2 cut(s) 736, 899
HpyCH4III ACNGT 5 cut(s) 275, 307, 586, 610, 1045
HpyCH4IV ACGT 1 cut(s) 613
HpyCH4V TGCA 6 cut(s) 81, 116, 350, 372, 507, 659
HpyF10VI GCNNNNNNNGC 5 cut(s) 420, 432, 488, 519, 794
HpyF3I CTNAG 3 cut(s) 227, 539, 900
HpySE526I ACGT 1 cut(s) 613
Hsp92II CATG 3 cut(s) 402, 708, 1086
Kzo9I GATC 4 cut(s) 381, 625, 842, 874
LmnI GCTCC 2 cut(s) 329, 800
Lsp1109I GCAGC 2 cut(s) 148, 435
LweI GCATC 2 cut(s) 544, 808
MaeI CTAG 2 cut(s) 86, 446
MaeII ACGT 1 cut(s) 613
MaeIII GTNAC 6 cut(s) 134, 688, 767, 808, 908, 1039
MalI GATC 4 cut(s) 383, 627, 844, 876
MboI GATC 4 cut(s) 381, 625, 842, 874
MflI RGATCY 3 cut(s) 625, 842, 874
MhlI GDGCHC 1 cut(s) 394
MluCI AATT 3 cut(s) 49, 830, 949
MmeI TCCRAC 2 cut(s) 265, 1030
MseI TTAA 1 cut(s) 915
MslI CAYNNNNRTG 2 cut(s) 297, 968
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 2 cut(s) 825, 879
Mva1269I GAATGC 1 cut(s) 437
MvaI CCWGG 2 cut(s) 825, 879
MwoI GCNNNNNNNGC 5 cut(s) 420, 432, 488, 519, 794
NcoI CCATGG 1 cut(s) 398
NdeII GATC 4 cut(s) 381, 625, 842, 874
NlaIII CATG 3 cut(s) 402, 708, 1086
NlaIV GGNNCC 4 cut(s) 325, 404, 521, 702
NmeAIII GCCGAG 2 cut(s) 373, 970
NmuCI GTSAC 1 cut(s) 1039
NspI RCATGY 1 cut(s) 1086
OliI CACNNNNGTG 1 cut(s) 968
PaeR7I CTCGAG 1 cut(s) 966
PaqCI CACCTGC 1 cut(s) 341
PciI ACATGT 1 cut(s) 1082
PctI GAATGC 1 cut(s) 437
PflMI CCANNNNNTGG 1 cut(s) 333
PkrI GCNGC 2 cut(s) 163, 425
PscI ACATGT 1 cut(s) 1082
PshAI GACNNNNGTC 1 cut(s) 1085
Psp124BI GAGCTC 1 cut(s) 394
Psp6I CCWGG 2 cut(s) 823, 877
PspGI CCWGG 2 cut(s) 823, 877
PspN4I GGNNCC 4 cut(s) 325, 404, 521, 702
PspPI GGNCC 1 cut(s) 15
PspXI VCTCGAGB 1 cut(s) 966
PstI CTGCAG 1 cut(s) 661
PsuI RGATCY 3 cut(s) 625, 842, 874
RsaI GTAC 5 cut(s) 56, 450, 457, 612, 642
RsaNI GTAC 5 cut(s) 55, 449, 456, 611, 641
RseI CAYNNNNRTG 2 cut(s) 297, 968
SacI GAGCTC 1 cut(s) 394
SaqAI TTAA 1 cut(s) 915
SatI GCNGC 2 cut(s) 162, 424
Sau3AI GATC 4 cut(s) 381, 625, 842, 874
Sau96I GGNCC 1 cut(s) 15
ScaI AGTACT 1 cut(s) 642
ScrFI CCNGG 2 cut(s) 825, 879
SduI GDGCHC 1 cut(s) 394
SfaNI GCATC 2 cut(s) 544, 808
SfcI CTRYAG 1 cut(s) 657
Sfr274I CTCGAG 1 cut(s) 966
SinI GGWCC 1 cut(s) 15
SlaI CTCGAG 1 cut(s) 966
SmiMI CAYNNNNRTG 2 cut(s) 297, 968
SmlI CTYRAG 1 cut(s) 966
SmoI CTYRAG 1 cut(s) 966
SpeI ACTAGT 1 cut(s) 445
Sse9I AATT 3 cut(s) 49, 830, 949
SspMI CTAG 2 cut(s) 86, 446
SstI GAGCTC 1 cut(s) 394
StyD4I CCNGG 2 cut(s) 823, 877
StyI CCWWGG 1 cut(s) 398
TaaI ACNGT 5 cut(s) 275, 307, 586, 610, 1045
TaiI ACGT 1 cut(s) 616
TaqI TCGA 2 cut(s) 295, 967
TasI AATT 3 cut(s) 49, 830, 949
TatI WGTACW 3 cut(s) 54, 448, 640
Tru1I TTAA 1 cut(s) 915
Tru9I TTAA 1 cut(s) 915
TscAI CASTG 3 cut(s) 178, 338, 416
TseFI GTSAC 1 cut(s) 1039
TseI GCWGC 2 cut(s) 161, 423
Tsp45I GTSAC 1 cut(s) 1039
TspDTI ATGAA 4 cut(s) 17, 755, 807, 906
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 3 cut(s) 178, 338, 416
Van91I CCANNNNNTGG 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 15
XagI CCTNNNNNAGG 1 cut(s) 481
XapI RAATTY 2 cut(s) 830, 949
XceI RCATGY 1 cut(s) 1086
XhoI CTCGAG 1 cut(s) 966
XspI CTAG 2 cut(s) 86, 446
ZrmI AGTACT 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.