Rh1DG061400

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0017412
Physical Location & Seq
Reverse (-)
10772385 .. 10802117
29733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG061400.1

Sequence Viewer

Length: 318 bp
ATGAGCTTGTTAGTTTGTAGAGCCATTGATGAAAACAGAAAGGTCCATATAGTATACATGGGCTCACTTCCCGCTAACCATTCGTATTCACCATTGTCTCACCACCTTAGTATGCTAGAAAGAGTTGTCAAGAATGGTTCAAATCTCCTGACGAGAAGTTACAAAAGGAGTTTCAATGGATTTGCTGCCATGCTCACTGACAATGAAAGACAAGCGCTTGCTAACATGAAGGAAGTTGCTGAATTTGAAAAGTCTGATCCTTGTACTGTGATGGACCCTCCTAACTGGACAGAAGCCAACTTTGCAGGCCCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

105

Amino Acids

11.77

Weight (kDa)

6.9

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 15 - 87 2.5e-13 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 54
AciI CCGC 1 cut(s) 72
AclWI GGATC 1 cut(s) 251
AcsI RAATTY 1 cut(s) 242
AfaI GTAC 1 cut(s) 265
AfeI AGCGCT 1 cut(s) 216
AgsI TTSAA 3 cut(s) 141, 175, 248
AluBI AGCT 1 cut(s) 6
AluI AGCT 1 cut(s) 6
Alw26I GTCTC 1 cut(s) 102
AlwI GGATC 1 cut(s) 251
AlwNI CAGNNNCTG 1 cut(s) 311
Aor51HI AGCGCT 1 cut(s) 216
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 1 cut(s) 185
ApoI RAATTY 1 cut(s) 242
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 3 cut(s) 43, 274, 308
AsuHPI GGTGA 2 cut(s) 81, 92
AvaII GGWCC 2 cut(s) 43, 274
BanII GRGCYC 1 cut(s) 65
BbvI GCAGC 1 cut(s) 172
BccI CCATC 1 cut(s) 265
BcoDI GTCTC 1 cut(s) 102
BfaI CTAG 1 cut(s) 116
BfoI RGCGCY 1 cut(s) 218
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
Bme18I GGWCC 2 cut(s) 43, 274
BmgT120I GGNCC 3 cut(s) 43, 274, 308
BmiI GGNNCC 1 cut(s) 276
Bse1I ACTGG 1 cut(s) 290
BseNI ACTGG 1 cut(s) 290
BseXI GCAGC 1 cut(s) 172
BshFI GGCC 1 cut(s) 309
BsmAI GTCTC 1 cut(s) 102
BsnI GGCC 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 1 cut(s) 256
BspACI CCGC 1 cut(s) 72
BspANI GGCC 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 276
BspPI GGATC 1 cut(s) 251
BsrI ACTGG 1 cut(s) 290
BssMI GATC 1 cut(s) 256
BssNAI GTATAC 1 cut(s) 55
Bst1107I GTATAC 1 cut(s) 55
Bst4CI ACNGT 1 cut(s) 268
BstAPI GCANNNNNTGC 1 cut(s) 311
BstC8I GCNNGC 2 cut(s) 219, 307
BstDEI CTNAG 1 cut(s) 107
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 1 cut(s) 259
BstMAI GTCTC 1 cut(s) 102
BstMBI GATC 1 cut(s) 256
BstMWI GCNNNNNNNGC 2 cut(s) 302, 311
BstV1I GCAGC 1 cut(s) 172
BstZ17I GTATAC 1 cut(s) 55
BsuRI GGCC 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 219, 307
CaiI CAGNNNCTG 1 cut(s) 311
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 3 cut(s) 43, 274, 308
Csp6I GTAC 1 cut(s) 264
CviAII CATG 4 cut(s) 58, 190, 226, 315
CviJI RGCY 5 cut(s) 6, 23, 63, 296, 309
CviKI_1 RGCY 5 cut(s) 6, 23, 63, 296, 309
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 1 cut(s) 107
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 65
Eco47I GGWCC 2 cut(s) 43, 274
Eco47III AGCGCT 1 cut(s) 216
EcoO109I RGGNCCY 1 cut(s) 308
EcoT38I GRGCYC 1 cut(s) 65
FaeI CATG 4 cut(s) 61, 193, 229, 318
FaiI YATR 8 cut(s) 48, 50, 55, 59, 113, 191, 227, 316
FatI CATG 4 cut(s) 57, 189, 225, 314
FauI CCCGC 1 cut(s) 79
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 186
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 1 cut(s) 116
GlaI GCGC 1 cut(s) 216
GluI GCNGC 1 cut(s) 186
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 1 cut(s) 309
HhaI GCGC 1 cut(s) 217
Hin1II CATG 4 cut(s) 61, 193, 229, 318
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HphI GGTGA 2 cut(s) 81, 92
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 1 cut(s) 256
Hpy188III TCNNGA 2 cut(s) 130, 148
Hpy8I GTNNAC 1 cut(s) 55
HpyAV CCTTC 1 cut(s) 223
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 2 cut(s) 305, 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 311
HpyF3I CTNAG 1 cut(s) 107
Hsp92II CATG 4 cut(s) 61, 193, 229, 318
HspAI GCGC 1 cut(s) 215
Kzo9I GATC 1 cut(s) 256
LpnPI CCDG 3 cut(s) 161, 271, 291
Lsp1109I GCAGC 1 cut(s) 172
MaeI CTAG 1 cut(s) 116
MaeIII GTNAC 1 cut(s) 158
MalI GATC 1 cut(s) 258
MboI GATC 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 65
MluCI AATT 1 cut(s) 242
MnlI CCTC 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 302, 311
NdeII GATC 1 cut(s) 256
NlaIII CATG 4 cut(s) 61, 193, 229, 318
NlaIV GGNNCC 1 cut(s) 276
PkrI GCNGC 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 276
PspPI GGNCC 3 cut(s) 43, 274, 308
PstNI CAGNNNCTG 1 cut(s) 311
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 1 cut(s) 256
Sau96I GGNCC 3 cut(s) 43, 274, 308
SduI GDGCHC 1 cut(s) 65
SetI ASST 3 cut(s) 8, 45, 108
SinI GGWCC 2 cut(s) 43, 274
Sse9I AATT 1 cut(s) 242
SsiI CCGC 1 cut(s) 72
SspMI CTAG 1 cut(s) 116
TaaI ACNGT 1 cut(s) 268
TasI AATT 1 cut(s) 242
TatI WGTACW 1 cut(s) 263
TscAI CASTG 1 cut(s) 202
TseI GCWGC 1 cut(s) 185
TspDTI ATGAA 3 cut(s) 45, 219, 242
TspRI CASTG 1 cut(s) 202
VpaK11BI GGWCC 2 cut(s) 43, 274
XapI RAATTY 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.