RchiOBHm_Chr1g0324021

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0017412
Physical Location & Seq
Forward (+)
11527584 .. 11527916
333 bp
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UTR
Exon/CDS
Intron
PRQ55387

Sequence Viewer

Length: 333 bp
ATGGCTAAGCATGGAGCTCTCCTATGTTCTTATGCTTTCACCATTCTCATACTCACTTTGATCTTATTGTGCAATGCTGTTGATGAAGATAGACATGCTCATGTTATGTACTTAGGGTCACTTCCAGATGACAAACTGTACTCACCGTTGTCTCACCACCTTAGCATTCTGCAAAGAGTAGTTGGGAGAGAATCTGCTGCAAATTTGTTAATAAAAAATTACAAAAGAAGTTTCAATGGATTTTCTGCCAATCTCACTGAACATGAAAAAGAAGAGCTTGCTAACATGAAGGAAGTAGTCTCTGTCTTTCCAAGCACAACTTTCCAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

110

Amino Acids

12.36

Weight (kDa)

6.57

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 41 - 110 1.2e-14 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 202
AfaI GTAC 2 cut(s) 110, 140
AgsI TTSAA 1 cut(s) 235
AluBI AGCT 2 cut(s) 17, 277
AluI AGCT 2 cut(s) 17, 277
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 2 cut(s) 156, 304
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 1 cut(s) 202
AsuHPI GGTGA 3 cut(s) 31, 135, 146
BanII GRGCYC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 184
BcoDI GTCTC 2 cut(s) 156, 304
BisI GCNGC 1 cut(s) 198
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 199
Bpu10I CCTNAGC 1 cut(s) 161
Bpu1102I GCTNAGC 1 cut(s) 6
Bse3DI GCAATG 1 cut(s) 79
BseMI GCAATG 1 cut(s) 79
BseXI GCAGC 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 19
BsmAI GTCTC 2 cut(s) 156, 304
BsmI GAATGC 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 1 cut(s) 60
Bsp1720I GCTNAGC 1 cut(s) 6
BspQI GCTCTTC 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 79
BssMI GATC 1 cut(s) 60
Bst4CI ACNGT 2 cut(s) 138, 147
Bst6I CTCTTC 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 3 cut(s) 6, 112, 161
BstKTI GATC 1 cut(s) 63
BstMAI GTCTC 2 cut(s) 156, 304
BstMBI GATC 1 cut(s) 60
BstNSI RCATGY 1 cut(s) 98
BstV1I GCAGC 1 cut(s) 184
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 279
Csp6I GTAC 2 cut(s) 109, 139
CviAII CATG 5 cut(s) 11, 95, 101, 263, 286
CviJI RGCY 3 cut(s) 5, 17, 277
CviKI_1 RGCY 3 cut(s) 5, 17, 277
CviQI GTAC 2 cut(s) 109, 139
DdeI CTNAG 3 cut(s) 6, 112, 161
DpnI GATC 1 cut(s) 62
DpnII GATC 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Ecl136II GAGCTC 1 cut(s) 17
Eco24I GRGCYC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 17
EcoICRI GAGCTC 1 cut(s) 17
EcoT38I GRGCYC 1 cut(s) 19
FaeI CATG 5 cut(s) 14, 98, 104, 266, 289
FaiI YATR 9 cut(s) 12, 25, 33, 50, 96, 102, 107, 264, 287
FalI AAGNNNNNCTT 3 cut(s) 261, 293, 304
FatI CATG 5 cut(s) 10, 94, 100, 262, 285
Fnu4HI GCNGC 1 cut(s) 198
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 1 cut(s) 198
GluI GCNGC 1 cut(s) 198
Hin1II CATG 5 cut(s) 14, 98, 104, 266, 289
HinfI GANTC 1 cut(s) 191
HphI GGTGA 3 cut(s) 31, 135, 146
Hpy188III TCNNGA 1 cut(s) 125
HpyAV CCTTC 1 cut(s) 283
HpyCH4III ACNGT 2 cut(s) 138, 147
HpyCH4V TGCA 3 cut(s) 72, 172, 200
HpyF3I CTNAG 3 cut(s) 6, 112, 161
Hsp92II CATG 5 cut(s) 14, 98, 104, 266, 289
Kzo9I GATC 1 cut(s) 60
LguI GCTCTTC 1 cut(s) 267
LmnI GCTCC 1 cut(s) 14
LpnPI CCDG 1 cut(s) 138
Lsp1109I GCAGC 1 cut(s) 184
MaeIII GTNAC 1 cut(s) 117
MalI GATC 1 cut(s) 62
MboI GATC 1 cut(s) 60
MboII GAAGA 2 cut(s) 98, 284
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 2 cut(s) 202, 217
MseI TTAA 2 cut(s) 209, 331
MslI CAYNNNNRTG 1 cut(s) 99
Mva1269I GAATGC 1 cut(s) 165
NdeII GATC 1 cut(s) 60
NlaIII CATG 5 cut(s) 14, 98, 104, 266, 289
NmuCI GTSAC 1 cut(s) 117
NspI RCATGY 1 cut(s) 98
PciSI GCTCTTC 1 cut(s) 267
PctI GAATGC 1 cut(s) 165
PfeI GAWTC 1 cut(s) 191
PkrI GCNGC 1 cut(s) 199
Psp124BI GAGCTC 1 cut(s) 19
RsaI GTAC 2 cut(s) 110, 140
RsaNI GTAC 2 cut(s) 109, 139
RseI CAYNNNNRTG 1 cut(s) 99
SacI GAGCTC 1 cut(s) 19
SapI GCTCTTC 1 cut(s) 267
SaqAI TTAA 2 cut(s) 209, 331
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 1 cut(s) 60
SduI GDGCHC 1 cut(s) 19
SetI ASST 3 cut(s) 19, 162, 279
SgeI CNNG 8 cut(s) 23, 107, 113, 137, 275, 290, 298, 324
SmiMI CAYNNNNRTG 1 cut(s) 99
Sse9I AATT 2 cut(s) 202, 217
SstI GAGCTC 1 cut(s) 19
TaaI ACNGT 2 cut(s) 138, 147
TasI AATT 2 cut(s) 202, 217
TatI WGTACW 2 cut(s) 108, 138
TfiI GAWTC 1 cut(s) 191
Tru1I TTAA 2 cut(s) 209, 331
Tru9I TTAA 2 cut(s) 209, 331
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 117
TseI GCWGC 1 cut(s) 197
Tsp45I GTSAC 1 cut(s) 117
TspDTI ATGAA 3 cut(s) 99, 279, 302
TspRI CASTG 1 cut(s) 262
XapI RAATTY 1 cut(s) 202
XceI RCATGY 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.