RchiOBHm_Chr1g0333821

GDSL esterase lipase At1g71250-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0014790
Physical Location & Seq
Reverse (-)
25970980 .. 25972726
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1302 bp
ATGAACCATCAATCATACTTGTTACGCCTATCGCCTAACCGATACACACAAACACCATGCACTGAAACTCAAATGCTGAACGCAACATTAGTATCAAGCAGTGAAATGCGAGCCACACAACAGAACCAAAGTAAGATGACAACAATGAAAGAAACCCTCTCTTCCAAAACAACGGGTTTGCTTCTCGTTCATCATCTAGCTCTATTGGTCATGGGGTTTATGGTTTTGGCTCAAGGGAAACTGAAACAGGCTTCGAGTGTCCAAAATTGTAGAAATGACTCTGCAGCATTCTACATATTGGGGGATTCCTCTGTTGATTGCGGAGACAACACGCTCTTTTACCCTCTCATTCACAGTTATCTGTCCTTGTATTCGTGTAATGGTTCCGACGGCTCAACTCTTATTCCCCATCTTCTTGCTGAGAAGATGGGCATGCCAAACATTTTACCATTCTACAGTCAAAATGGATCCATTAATGGAATACAAAGAGGCCTTAACTTCGGTTCAGCACATGCAACGATCTTGAATCACGGCAGCCAGAGTTATCAGTCTGTGAACCAGCAGCTCCGCCAAGTTTTTGATTCTATGCAACTCTTACAATTGCAGCTTAGCGAGGACAGAGCTCTCAGTTTCATCAAATCTTCCATCTTCTACCTCTCCTTTGGCAAAGATGACTTCATAGAGCTCTTCCTACGCAATGCCTCAGGTACTGCAAAACAGAATAATACTGATCAAGAATTTTCACACATTTTAGCAGATCAGATGGTACATGTTATACGGAACCTTTATGACATGAATGTGAGGAGGATCATATGCATGGGAATATTGCCGTTGGGATGCACCCCGCGGGTGTTATTGAACCAGTACAATTCCACAGCCCATCCAAATGAAGATGATGATGATGATGTTCCAAGGGGATGTGTAGAGGAGATCAATGCACAGGTCTTAGAGTACAACAGAGAGCTGAATGAGCAAATCTCTGAGCTTCATGCAGAGTTGCCTGATGCACAGCTTCTGTTTTGTGATGTTTACCAAGGACTTGCAACGATCATAAACAACCCAGAACAATATGGATTTGAGGATGTAAAGAATGCTTGCTGCGGGCTTGGTATGTATGGAGCAGAAATTGGATGCCTCTCTAAAGATATGGCATGTGACAAAGAAGCAAGTCATGTGTGGTGGGATTTGTACAATCCTACAAAAGCAGTGAACTCATTGTTGGCTGATTCAGCCTGGTCGAGCTATCCGCTTCGGATTTGTCGTCCTATGAGCATCCAGGAATTTTTTAGTGCTTCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

433

Amino Acids

48.42

Weight (kDa)

5.19

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 98 - 409 2e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06121 FvH4_7g06190
malus_domestica MD00G1178200.v1.1
prunus_persica Prupe.6G152900_v2.0.a1
pyrus_communis pycom11g15560
rosa_chinensis RchiOBHm_Chr1g0333821
rosa_laevigata RLG00000029528
rosa_multiflora Rmu_sc0000026.1_g000017 Rmu_sc0007177.1_g000005
rosa_roxburghii Rroxscaffold_4G00317200
rosa_rugosa Rorug01G0112100
rosa_samantha Rh1AG134500 Rh1AG134600 Rh1BG102800 Rh1CG127900 Rh1DG140000
rosa_wichuraiana Rw1G011220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 847
AciI CCGC 6 cut(s) 321, 568, 845, 847, 1101, 1247
AclWI GGATC 3 cut(s) 462, 475, 815
AcsI RAATTY 2 cut(s) 737, 1280
AcuI CTGAAG 1 cut(s) 1278
AfaI GTAC 5 cut(s) 709, 768, 866, 953, 1190
AflIII ACRYGT 1 cut(s) 769
AgsI TTSAA 2 cut(s) 526, 859
AjnI CCWGG 2 cut(s) 1232, 1275
AjuI GAANNNNNNNTTGG 2 cut(s) 1202, 1234
AluBI AGCT 9 cut(s) 200, 565, 607, 623, 685, 964, 985, 1012, 1242
AluI AGCT 9 cut(s) 200, 565, 607, 623, 685, 964, 985, 1012, 1242
Alw21I GWGCWC 2 cut(s) 625, 687
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 3 cut(s) 462, 475, 815
AlwNI CAGNNNCTG 2 cut(s) 710, 1015
AoxI GGCC 1 cut(s) 490
ApeKI GCWGC 5 cut(s) 284, 534, 562, 604, 1098
ApoI RAATTY 2 cut(s) 737, 1280
AseI ATTAAT 1 cut(s) 474
AxyI CCTNAGG 1 cut(s) 703
BaeI ACNNNNGTAYC 2 cut(s) 75, 108
BamHI GGATCC 1 cut(s) 467
BanII GRGCYC 2 cut(s) 625, 687
Bbv12I GWGCWC 2 cut(s) 625, 687
BbvI GCAGC 5 cut(s) 296, 546, 574, 616, 1085
BccI CCATC 6 cut(s) 15, 417, 421, 653, 757, 888
BceAI ACGGC 3 cut(s) 406, 547, 814
BciT130I CCWGG 2 cut(s) 1234, 1277
BclI TGATCA 1 cut(s) 730
BcoDI GTCTC 1 cut(s) 318
BfaI CTAG 1 cut(s) 197
BfmI CTRYAG 2 cut(s) 282, 454
BisI GCNGC 5 cut(s) 285, 535, 563, 605, 1099
BlpI GCTNAGC 1 cut(s) 608
BlsI GCNGC 5 cut(s) 286, 536, 564, 606, 1100
Bme1390I CCNGG 2 cut(s) 1234, 1277
BmiI GGNNCC 3 cut(s) 385, 469, 782
BmrFI CCNGG 2 cut(s) 1234, 1277
BmsI GCATC 4 cut(s) 827, 994, 1121, 1281
BplI GAGNNNNNCTC 2 cut(s) 962, 994
Bpu1102I GCTNAGC 1 cut(s) 608
BpuEI CTTGAG 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 845, 911, 1033
Bse1I ACTGG 1 cut(s) 862
Bse21I CCTNAGG 1 cut(s) 703
Bse3DI GCAATG 1 cut(s) 703
BseBI CCWGG 2 cut(s) 1234, 1277
BseDI CCNNGG 3 cut(s) 845, 911, 1033
BseGI GGATG 6 cut(s) 842, 880, 923, 1087, 1136, 1272
BseMI GCAATG 1 cut(s) 703
BseMII CTCAG 4 cut(s) 411, 640, 717, 972
BseNI ACTGG 1 cut(s) 862
BseRI GAGGAG 2 cut(s) 817, 941
BseXI GCAGC 5 cut(s) 296, 546, 574, 616, 1085
Bsh1236I CGCG 1 cut(s) 847
BshFI GGCC 1 cut(s) 492
BsiHKAI GWGCWC 2 cut(s) 625, 687
BsmAI GTCTC 1 cut(s) 318
BsmI GAATGC 2 cut(s) 287, 1096
BsnI GGCC 1 cut(s) 492
Bsp1286I GDGCHC 2 cut(s) 625, 687
Bsp1407I TGTACA 1 cut(s) 1188
Bsp143I GATC 7 cut(s) 467, 519, 730, 757, 807, 930, 1047
Bsp1720I GCTNAGC 1 cut(s) 608
BspACI CCGC 6 cut(s) 321, 568, 845, 847, 1101, 1247
BspANI GGCC 1 cut(s) 492
BspCNI CTCAG 4 cut(s) 412, 639, 716, 973
BspFNI CGCG 1 cut(s) 847
BspLI GGNNCC 3 cut(s) 385, 469, 782
BspMAI CTGCAG 1 cut(s) 286
BspPI GGATC 3 cut(s) 462, 475, 815
BspQI GCTCTTC 1 cut(s) 692
BsrDI GCAATG 1 cut(s) 703
BsrGI TGTACA 1 cut(s) 1188
BsrI ACTGG 1 cut(s) 862
BssECI CCNNGG 3 cut(s) 845, 911, 1033
BssMI GATC 7 cut(s) 467, 519, 730, 757, 807, 930, 1047
BssT1I CCWWGG 2 cut(s) 911, 1033
Bst2UI CCWGG 2 cut(s) 1234, 1277
Bst4CI ACNGT 2 cut(s) 356, 458
Bst6I CTCTTC 2 cut(s) 166, 692
BstAUI TGTACA 1 cut(s) 1188
BstC8I GCNNGC 4 cut(s) 111, 434, 1096, 1103
BstDEI CTNAG 6 cut(s) 420, 608, 626, 703, 946, 981
BstDSI CCRYGG 1 cut(s) 845
BstF5I GGATG 6 cut(s) 842, 880, 923, 1087, 1136, 1272
BstFNI CGCG 1 cut(s) 847
BstKTI GATC 7 cut(s) 470, 522, 733, 760, 810, 933, 1050
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 7 cut(s) 467, 519, 730, 757, 807, 930, 1047
BstMWI GCNNNNNNNGC 2 cut(s) 970, 1229
BstNI CCWGG 2 cut(s) 1234, 1277
BstNSI RCATGY 4 cut(s) 436, 515, 773, 1155
BstSCI CCNGG 2 cut(s) 1232, 1275
BstSFI CTRYAG 2 cut(s) 282, 454
BstUI CGCG 1 cut(s) 847
BstV1I GCAGC 5 cut(s) 296, 546, 574, 616, 1085
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
Bsu36I CCTNAGG 1 cut(s) 703
BsuRI GGCC 1 cut(s) 492
BtgI CCRYGG 1 cut(s) 845
BtsCI GGATG 6 cut(s) 842, 880, 923, 1087, 1136, 1272
BtsI GCAGTG 2 cut(s) 106, 1212
BtsIMutI CAGTG 3 cut(s) 60, 106, 1212
Cac8I GCNNGC 4 cut(s) 111, 434, 1096, 1103
CaiI CAGNNNCTG 2 cut(s) 710, 1015
Cfr42I CCGCGG 1 cut(s) 848
Csp6I GTAC 5 cut(s) 708, 767, 865, 952, 1189
CviQI GTAC 5 cut(s) 708, 767, 865, 952, 1189
DdeI CTNAG 6 cut(s) 420, 608, 626, 703, 946, 981
DpnI GATC 7 cut(s) 469, 521, 732, 759, 809, 932, 1049
DpnII GATC 7 cut(s) 467, 519, 730, 757, 807, 930, 1047
Eam1104I CTCTTC 2 cut(s) 166, 692
EarI CTCTTC 2 cut(s) 166, 692
EciI GGCGGA 1 cut(s) 557
Ecl136II GAGCTC 2 cut(s) 623, 685
Eco130I CCWWGG 2 cut(s) 911, 1033
Eco147I AGGCCT 1 cut(s) 492
Eco24I GRGCYC 2 cut(s) 625, 687
Eco53kI GAGCTC 2 cut(s) 623, 685
Eco57I CTGAAG 1 cut(s) 1278
Eco81I CCTNAGG 1 cut(s) 703
EcoICRI GAGCTC 2 cut(s) 623, 685
EcoRII CCWGG 2 cut(s) 1232, 1275
EcoT14I CCWWGG 2 cut(s) 911, 1033
EcoT22I ATGCAT 1 cut(s) 818
EcoT38I GRGCYC 2 cut(s) 625, 687
ErhI CCWWGG 2 cut(s) 911, 1033
FauI CCCGC 3 cut(s) 840, 852, 1094
FauNDI CATATG 1 cut(s) 812
FbaI TGATCA 1 cut(s) 730
Fnu4HI GCNGC 5 cut(s) 285, 535, 563, 605, 1099
FokI GGATG 6 cut(s) 849, 867, 930, 1094, 1143, 1259
FriOI GRGCYC 2 cut(s) 625, 687
Fsp4HI GCNGC 5 cut(s) 285, 535, 563, 605, 1099
FspBI CTAG 1 cut(s) 197
GluI GCNGC 5 cut(s) 285, 535, 563, 605, 1099
HaeIII GGCC 1 cut(s) 492
HinfI GANTC 5 cut(s) 278, 305, 526, 581, 1226
Hpy166II GTNNAC 3 cut(s) 556, 1030, 1210
Hpy188I TCNGA 4 cut(s) 388, 762, 982, 1254
Hpy188III TCNNGA 2 cut(s) 523, 734
Hpy8I GTNNAC 3 cut(s) 556, 1030, 1210
Hpy99I CGWCG 1 cut(s) 392
HpyCH4III ACNGT 2 cut(s) 356, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 970, 1229
HpyF3I CTNAG 6 cut(s) 420, 608, 626, 703, 946, 981
Ksp22I TGATCA 1 cut(s) 730
KspI CCGCGG 1 cut(s) 848
Kzo9I GATC 7 cut(s) 467, 519, 730, 757, 807, 930, 1047
LguI GCTCTTC 1 cut(s) 692
LmnI GCTCC 2 cut(s) 570, 1118
Lsp1109I GCAGC 5 cut(s) 296, 546, 574, 616, 1085
LweI GCATC 4 cut(s) 827, 994, 1121, 1281
MaeI CTAG 1 cut(s) 197
MaeIII GTNAC 2 cut(s) 21, 1154
MalI GATC 7 cut(s) 469, 521, 732, 759, 809, 932, 1049
MboI GATC 7 cut(s) 467, 519, 730, 757, 807, 930, 1047
MboII GAAGA 7 cut(s) 153, 404, 436, 633, 640, 679, 902
MfeI CAATTG 1 cut(s) 599
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 2 cut(s) 625, 687
MluCI AATT 6 cut(s) 265, 599, 737, 868, 1125, 1280
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 411
Mph1103I ATGCAT 1 cut(s) 818
MseI TTAA 3 cut(s) 474, 495, 1300
MslI CAYNNNNRTG 3 cut(s) 797, 815, 885
MspA1I CMGCKG 1 cut(s) 847
MspR9I CCNGG 2 cut(s) 1234, 1277
MunI CAATTG 1 cut(s) 599
Mva1269I GAATGC 2 cut(s) 287, 1096
MvaI CCWGG 2 cut(s) 1234, 1277
MvnI CGCG 1 cut(s) 847
MwoI GCNNNNNNNGC 2 cut(s) 970, 1229
NdeI CATATG 1 cut(s) 812
NdeII GATC 7 cut(s) 467, 519, 730, 757, 807, 930, 1047
NlaIV GGNNCC 3 cut(s) 385, 469, 782
NmuCI GTSAC 1 cut(s) 1154
NsiI ATGCAT 1 cut(s) 818
NspI RCATGY 4 cut(s) 436, 515, 773, 1155
PaeI GCATGC 1 cut(s) 436
PceI AGGCCT 1 cut(s) 492
PciI ACATGT 1 cut(s) 769
PciSI GCTCTTC 1 cut(s) 692
PcsI WCGNNNNNNNCGW 1 cut(s) 1258
PctI GAATGC 2 cut(s) 287, 1096
PfeI GAWTC 4 cut(s) 305, 526, 581, 1226
PfoI TCCNGGA 1 cut(s) 1275
PkrI GCNGC 5 cut(s) 286, 536, 564, 606, 1100
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PscI ACATGT 1 cut(s) 769
PshBI ATTAAT 1 cut(s) 474
Psp124BI GAGCTC 2 cut(s) 625, 687
Psp6I CCWGG 2 cut(s) 1232, 1275
PspGI CCWGG 2 cut(s) 1232, 1275
PspN4I GGNNCC 3 cut(s) 385, 469, 782
PstI CTGCAG 1 cut(s) 286
PstNI CAGNNNCTG 2 cut(s) 710, 1015
PsuI RGATCY 1 cut(s) 467
RsaI GTAC 5 cut(s) 709, 768, 866, 953, 1190
RsaNI GTAC 5 cut(s) 708, 767, 865, 952, 1189
RseI CAYNNNNRTG 3 cut(s) 797, 815, 885
SacI GAGCTC 2 cut(s) 625, 687
SacII CCGCGG 1 cut(s) 848
SapI GCTCTTC 1 cut(s) 692
SaqAI TTAA 3 cut(s) 474, 495, 1300
SatI GCNGC 5 cut(s) 285, 535, 563, 605, 1099
Sau3AI GATC 7 cut(s) 467, 519, 730, 757, 807, 930, 1047
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 2 cut(s) 1234, 1277
SduI GDGCHC 2 cut(s) 625, 687
SfaNI GCATC 4 cut(s) 827, 994, 1121, 1281
SfcI CTRYAG 2 cut(s) 282, 454
Sfr303I CCGCGG 1 cut(s) 848
SgrBI CCGCGG 1 cut(s) 848
SmiMI CAYNNNNRTG 3 cut(s) 797, 815, 885
SmlI CTYRAG 1 cut(s) 231
SmoI CTYRAG 1 cut(s) 231
SphI GCATGC 1 cut(s) 436
Sse9I AATT 6 cut(s) 265, 599, 737, 868, 1125, 1280
SseBI AGGCCT 1 cut(s) 492
SsiI CCGC 6 cut(s) 321, 568, 845, 847, 1101, 1247
SspI AATATT 1 cut(s) 825
SspMI CTAG 1 cut(s) 197
SstI GAGCTC 2 cut(s) 625, 687
StuI AGGCCT 1 cut(s) 492
StyD4I CCNGG 2 cut(s) 1232, 1275
StyI CCWWGG 2 cut(s) 911, 1033
TaaI ACNGT 2 cut(s) 356, 458
TaqI TCGA 2 cut(s) 254, 1238
TasI AATT 6 cut(s) 265, 599, 737, 868, 1125, 1280
TatI WGTACW 3 cut(s) 864, 951, 1188
TfiI GAWTC 4 cut(s) 305, 526, 581, 1226
Tru1I TTAA 3 cut(s) 474, 495, 1300
Tru9I TTAA 3 cut(s) 474, 495, 1300
TscAI CASTG 3 cut(s) 67, 106, 1212
TseFI GTSAC 1 cut(s) 1154
TseI GCWGC 5 cut(s) 284, 534, 562, 604, 1098
Tsp45I GTSAC 1 cut(s) 1154
TspDTI ATGAA 8 cut(s) 17, 161, 179, 622, 667, 809, 903, 977
TspGWI ACGGA 1 cut(s) 793
TspRI CASTG 3 cut(s) 67, 106, 1212
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 2 cut(s) 737, 1280
XceI RCATGY 4 cut(s) 436, 515, 773, 1155
XspI CTAG 1 cut(s) 197
Zsp2I ATGCAT 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.