RchiOBHm_Chr1g0349971

3-phosphoshikimate 1-carboxyvinyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004684
Physical Location & Seq
Forward (+)
43114717 .. 43119409
4693 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1566 bp
ATGGCCCAAGTGAGCAAAATCTGTGGCAATGGAGCTCAAAGCATCAATCTTTTGCCCAATGTCTCCAAACCCCAGATACCCAAATCGTTAAATTCAATCTCTTTGAAGTCACAGTTTTTGGGTTCTTCGAATTCTGTGAGTTTGAAGATTAAAAATGGGTTTGTGGGTGGTAAAGCCAGGGCTGGTGGGCTTAGAGTTTCGGCTTCAGTTGTTACAGCAGAGAAGCCGTCGACGGTGCCGGAGATTGTGTTGCAACCCATCAAAGAAATTTCGGGTACCATAACTTTGCCGGGTTCTAAGTCCTTGTCCAATCGGATTCTGCTTATTGCTGCTCTTTCTGAGGGAACAACTGTTGTAGACAACTTACTGGATAGTGATGATATCAATTATATGCTTGGTGCGTTGAAAACGCTTGGGCTAAATGTGGAAGATGACAAGGCAAACAAAAGAGCTGTTGTGGAGGGTTGTGGTGGTCAGTTTCCGATGGGGAATAAATCCGGAGAGGAAGTTCAACTTTTCCTTGGAAATGCTGGAACAGCAATGAGGCCTTTGACTGCGGCAGTAACTGCTGCTGGTGGATATTCTAGGTATGTACTTGATGGGGTGCCCCGAATGAGGGAGAGACCAATTGGAGATTTGGTCACTGGTCTTAAGCAGCTTGGTGCAGATGTTGACTGTTTTCTTGGAACAGATTGTCCTCCTGTTCGTGTAATTGGAAAGGGAGGCCTTCCAGGAGGCAAGGTGAAACTCTCTGGATCAATCAGTAGTCAATATTTGACTTCTTTGCTTATGGCGGCTCCTTTGGCTCTGGGAGATGTTGAAGTTGAGATTATTGATAAGCTGATTTCCATTCCATATGTTGAAATGACTTTGAAATTGATGGAACGCTTTGGGGTCACAGTGGAACATAGTGATAGCTGGGATCGATTTTTGGTCCGAGGAGGTCAAAAATACAAGTCTCCTGGAAAAGCCTTTGTCGAAGGTGATGCTTCAAGTGCTAGTTACTTTCTAGCTGGTGCTGCAGTAACGGGTGGGACTGTAACTGTTGAAGGCTGTGGCACAAGCAGTTTACAGGGAGATGTAAAATTCGCTGAAGTTCTTGAAAAGATGGGCGCTGAAGTTACCTGGACAGAGAACAGTGTCACCGTCACAGGACCTCCACGACATTCTTCTGGAAGAAAGCACTTGAAAGCTGTTGATGTCAACATGAACAAGATGCCAGATGTTGCCATGACTCTTGCTGTAGTTGCTCTTTTTGCTGATGGTCCAACTGCCATAAGAGATGTGGCAAGCTGGAGAGTGAAGGAGACAGAAAGGATGATTGCCATTTGCACCGAACTCAGGAAGTTGGGAGCAACTGTTGAAGAAGGGCCTGATTACTGTGTAATCACACCACCGGAAAAACTAAATGTAACAGCCATAGACACATACGATGACCACAGAATGGCCATGGCATTCTCCCTTGCTGCCTGTGGAGATGTTCCCGTTACCATCAAGGATCCCGGTTGCACCAGAAAAACATTCCCCAACTACTTCGAAGTCCTGGAGAAGTTTACAAAACATTAA

Protein Analysis

521

Amino Acids

55.19

Weight (kDa)

7.5

Isoelectric Point (pI)

26.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 85 - 515 7.9e-141 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 3 cut(s) 235, 275, 604
AccB7I CCANNNNNTGG 1 cut(s) 1444
AccI GTMKAC 2 cut(s) 230, 357
AccIII TCCGGA 1 cut(s) 497
AciI CCGC 2 cut(s) 557, 794
AclWI GGATC 4 cut(s) 763, 930, 1493, 1506
AcoI YGGCCR 1 cut(s) 1446
AcsI RAATTY 4 cut(s) 91, 130, 267, 1085
AcuI CTGAAG 3 cut(s) 189, 1113, 1137
AfaI GTAC 2 cut(s) 277, 594
AfiI CCNNNNNNNGG 4 cut(s) 615, 616, 1341, 1444
AflII CTTAAG 1 cut(s) 650
AjnI CCWGG 5 cut(s) 176, 730, 961, 1124, 1542
AloI GAACNNNNNNTCC 4 cut(s) 492, 524, 679, 711
AluBI AGCT 8 cut(s) 35, 452, 658, 841, 918, 1013, 1193, 1293
AluI AGCT 8 cut(s) 35, 452, 658, 841, 918, 1013, 1193, 1293
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 4 cut(s) 67, 616, 963, 1301
AlwI GGATC 4 cut(s) 763, 930, 1493, 1506
AlwNI CAGNNNCTG 1 cut(s) 566
Aor13HI TCCGGA 1 cut(s) 497
AoxI GGCC 5 cut(s) 3, 545, 724, 1370, 1446
ApeKI GCWGC 5 cut(s) 329, 569, 655, 1019, 1466
ApoI RAATTY 4 cut(s) 91, 130, 267, 1085
Asp718I GGTACC 1 cut(s) 275
AspLEI GCGC 1 cut(s) 1115
AspS9I GGNCC 5 cut(s) 4, 934, 1154, 1265, 1370
AsuC2I CCSGG 2 cut(s) 291, 1503
AsuHPI GGTGA 3 cut(s) 754, 995, 1135
AsuII TTCGAA 2 cut(s) 128, 1536
AvaII GGWCC 3 cut(s) 934, 1154, 1265
BaeGI GKGCMC 1 cut(s) 609
BalI TGGCCA 1 cut(s) 1448
BamHI GGATCC 1 cut(s) 1498
BanI GGYRCC 3 cut(s) 235, 275, 604
BanII GRGCYC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 37
BbvI GCAGC 5 cut(s) 316, 556, 667, 1006, 1453
BccI CCATC 7 cut(s) 266, 478, 593, 874, 1102, 1256, 1499
BceAI ACGGC 1 cut(s) 211
BcgI CGANNNNNNTGC 2 cut(s) 968, 1002
BciT130I CCWGG 5 cut(s) 178, 732, 963, 1126, 1544
BcnI CCSGG 2 cut(s) 291, 1503
BcoDI GTCTC 4 cut(s) 67, 616, 963, 1301
BfaI CTAG 3 cut(s) 585, 999, 1010
BfmI CTRYAG 2 cut(s) 1020, 1242
BfoI RGCGCY 1 cut(s) 1116
BfrI CTTAAG 1 cut(s) 650
BisI GCNGC 7 cut(s) 330, 558, 570, 656, 795, 1020, 1467
BlsI GCNGC 7 cut(s) 331, 559, 571, 657, 796, 1021, 1468
Bme1390I CCNGG 7 cut(s) 178, 291, 732, 963, 1126, 1503, 1544
Bme18I GGWCC 3 cut(s) 934, 1154, 1265
BmgT120I GGNCC 5 cut(s) 4, 934, 1154, 1265, 1370
BmiI GGNNCC 5 cut(s) 237, 277, 606, 798, 1500
BmrFI CCNGG 7 cut(s) 178, 291, 732, 963, 1126, 1503, 1544
BmsI GCATC 3 cut(s) 51, 976, 1206
BpmI CTGGAG 2 cut(s) 1315, 1565
Bpu14I TTCGAA 2 cut(s) 128, 1536
BpuMI CCSGG 2 cut(s) 291, 1503
Bsa29I ATCGAT 1 cut(s) 925
BsaI GGTCTC 1 cut(s) 616
BsaJI CCNNGG 4 cut(s) 177, 520, 937, 1449
BsaWI WCCGGW 2 cut(s) 497, 1396
Bsc4I CCNNNNNNNGG 4 cut(s) 615, 616, 1341, 1444
Bse1I ACTGG 2 cut(s) 372, 649
Bse3DI GCAATG 2 cut(s) 34, 546
BseAI TCCGGA 1 cut(s) 497
BseBI CCWGG 5 cut(s) 178, 732, 963, 1126, 1544
BseCI ATCGAT 1 cut(s) 925
BseDI CCNNGG 4 cut(s) 177, 520, 937, 1449
BseGI GGATG 1 cut(s) 1323
BseLI CCNNNNNNNGG 4 cut(s) 615, 616, 1341, 1444
BseMI GCAATG 2 cut(s) 34, 546
BseMII CTCAG 2 cut(s) 330, 1354
BseNI ACTGG 2 cut(s) 372, 649
BseRI GAGGAG 1 cut(s) 954
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 5 cut(s) 316, 556, 667, 1006, 1453
BseYI CCCAGC 1 cut(s) 918
BsgI GTGCAG 1 cut(s) 684
BshFI GGCC 5 cut(s) 5, 547, 726, 1372, 1448
BshNI GGYRCC 3 cut(s) 235, 275, 604
BshVI ATCGAT 1 cut(s) 925
BsiHKAI GWGCWC 1 cut(s) 37
BsiSI CCGG 5 cut(s) 239, 290, 498, 1397, 1503
BslFI GGGAC 1 cut(s) 1048
BslI CCNNNNNNNGG 4 cut(s) 615, 616, 1341, 1444
BsmAI GTCTC 4 cut(s) 67, 616, 963, 1301
BsmFI GGGAC 1 cut(s) 1048
BsmI GAATGC 1 cut(s) 1454
BsnI GGCC 5 cut(s) 5, 547, 726, 1372, 1448
Bso31I GGTCTC 1 cut(s) 616
Bsp119I TTCGAA 2 cut(s) 128, 1536
Bsp1286I GDGCHC 2 cut(s) 37, 609
Bsp13I TCCGGA 1 cut(s) 497
Bsp143I GATC 3 cut(s) 755, 922, 1498
Bsp19I CCATGG 1 cut(s) 1449
BspACI CCGC 2 cut(s) 557, 794
BspANI GGCC 5 cut(s) 5, 547, 726, 1372, 1448
BspCNI CTCAG 2 cut(s) 331, 1353
BspDI ATCGAT 1 cut(s) 925
BspEI TCCGGA 1 cut(s) 497
BspLI GGNNCC 5 cut(s) 237, 277, 606, 798, 1500
BspMAI CTGCAG 1 cut(s) 1024
BspPI GGATC 4 cut(s) 763, 930, 1493, 1506
BspT104I TTCGAA 2 cut(s) 128, 1536
BspT107I GGYRCC 3 cut(s) 235, 275, 604
BspTI CTTAAG 1 cut(s) 650
BspTNI GGTCTC 1 cut(s) 616
BsrDI GCAATG 2 cut(s) 34, 546
BsrI ACTGG 2 cut(s) 372, 649
BssECI CCNNGG 4 cut(s) 177, 520, 937, 1449
BssMI GATC 3 cut(s) 755, 922, 1498
BssT1I CCWWGG 2 cut(s) 520, 1449
Bst2UI CCWGG 5 cut(s) 178, 732, 963, 1126, 1544
BstAFI CTTAAG 1 cut(s) 650
BstAPI GCANNNNNTGC 1 cut(s) 566
BstBI TTCGAA 2 cut(s) 128, 1536
BstC8I GCNNGC 1 cut(s) 1291
BstDEI CTNAG 4 cut(s) 191, 297, 339, 1340
BstDSI CCRYGG 1 cut(s) 1449
BstF5I GGATG 1 cut(s) 1323
BstH2I RGCGCY 1 cut(s) 1116
BstHHI GCGC 1 cut(s) 1115
BstKTI GATC 3 cut(s) 758, 925, 1501
BstMAI GTCTC 4 cut(s) 67, 616, 963, 1301
BstMBI GATC 3 cut(s) 755, 922, 1498
BstMWI GCNNNNNNNGC 7 cut(s) 536, 566, 803, 995, 1019, 1247, 1256
BstNI CCWGG 5 cut(s) 178, 732, 963, 1126, 1544
BstSCI CCNGG 7 cut(s) 176, 289, 730, 961, 1124, 1501, 1542
BstSFI CTRYAG 2 cut(s) 1020, 1242
BstSLI GKGCMC 1 cut(s) 609
BstV1I GCAGC 5 cut(s) 316, 556, 667, 1006, 1453
BstX2I RGATCY 1 cut(s) 1498
BstYI RGATCY 1 cut(s) 1498
Bsu15I ATCGAT 1 cut(s) 925
BsuRI GGCC 5 cut(s) 5, 547, 726, 1372, 1448
BsuTUI ATCGAT 1 cut(s) 925
BtgI CCRYGG 1 cut(s) 1449
BtsCI GGATG 1 cut(s) 1323
BtsIMutI CAGTG 3 cut(s) 642, 906, 1144
Cac8I GCNNGC 1 cut(s) 1291
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 1 cut(s) 1115
Cfr13I GGNCC 5 cut(s) 4, 934, 1154, 1265, 1370
ClaI ATCGAT 1 cut(s) 925
Csp6I GTAC 2 cut(s) 276, 593
CspCI CAANNNNNGTGG 2 cut(s) 4, 39
CviAII CATG 3 cut(s) 1207, 1231, 1450
CviQI GTAC 2 cut(s) 276, 593
DdeI CTNAG 4 cut(s) 191, 297, 339, 1340
DpnI GATC 3 cut(s) 757, 924, 1500
DpnII GATC 3 cut(s) 755, 922, 1498
EaeI YGGCCR 1 cut(s) 1446
Ecl136II GAGCTC 1 cut(s) 35
Eco130I CCWWGG 2 cut(s) 520, 1449
Eco147I AGGCCT 2 cut(s) 547, 726
Eco24I GRGCYC 1 cut(s) 37
Eco31I GGTCTC 1 cut(s) 616
Eco32I GATATC 1 cut(s) 382
Eco47I GGWCC 3 cut(s) 934, 1154, 1265
Eco53kI GAGCTC 1 cut(s) 35
Eco57I CTGAAG 3 cut(s) 189, 1113, 1137
EcoICRI GAGCTC 1 cut(s) 35
EcoO109I RGGNCCY 2 cut(s) 1154, 1370
EcoRI GAATTC 1 cut(s) 130
EcoRII CCWGG 5 cut(s) 176, 730, 961, 1124, 1542
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 2 cut(s) 520, 1449
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 2 cut(s) 520, 1449
FaeI CATG 3 cut(s) 1210, 1234, 1453
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 1 cut(s) 1048
FatI CATG 3 cut(s) 1206, 1230, 1449
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 2 cut(s) 230, 357
Fnu4HI GCNGC 7 cut(s) 330, 558, 570, 656, 795, 1020, 1467
FokI GGATG 1 cut(s) 1330
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 7 cut(s) 330, 558, 570, 656, 795, 1020, 1467
FspBI CTAG 3 cut(s) 585, 999, 1010
GlaI GCGC 1 cut(s) 1114
GluI GCNGC 7 cut(s) 330, 558, 570, 656, 795, 1020, 1467
GsaI CCCAGC 1 cut(s) 922
GsuI CTGGAG 2 cut(s) 1315, 1565
HaeII RGCGCY 1 cut(s) 1116
HaeIII GGCC 5 cut(s) 5, 547, 726, 1372, 1448
HapII CCGG 5 cut(s) 239, 290, 498, 1397, 1503
HhaI GCGC 1 cut(s) 1115
Hin1II CATG 3 cut(s) 1210, 1234, 1453
Hin6I GCGC 1 cut(s) 1113
HinP1I GCGC 1 cut(s) 1113
HincII GTYRAC 3 cut(s) 231, 673, 1204
HindII GTYRAC 3 cut(s) 231, 673, 1204
HinfI GANTC 2 cut(s) 316, 1234
HpaII CCGG 5 cut(s) 239, 290, 498, 1397, 1503
HphI GGTGA 3 cut(s) 754, 995, 1135
Hpy166II GTNNAC 6 cut(s) 231, 358, 673, 1070, 1204, 1554
Hpy188I TCNGA 4 cut(s) 315, 340, 483, 938
Hpy188III TCNNGA 5 cut(s) 498, 753, 1100, 1173, 1342
Hpy8I GTNNAC 6 cut(s) 231, 358, 673, 1070, 1204, 1554
Hpy99I CGWCG 2 cut(s) 232, 235
HpyAV CCTTC 5 cut(s) 737, 974, 1043, 1297, 1361
HpyCH4V TGCA 5 cut(s) 253, 665, 1022, 1332, 1509
HpyF10VI GCNNNNNNNGC 7 cut(s) 536, 566, 803, 995, 1019, 1247, 1256
HpyF3I CTNAG 4 cut(s) 191, 297, 339, 1340
Hsp92II CATG 3 cut(s) 1210, 1234, 1453
HspAI GCGC 1 cut(s) 1113
Kpn2I TCCGGA 1 cut(s) 497
KpnI GGTACC 1 cut(s) 279
Kzo9I GATC 3 cut(s) 755, 922, 1498
LmnI GCTCC 3 cut(s) 32, 802, 1352
Lsp1109I GCAGC 5 cut(s) 316, 556, 667, 1006, 1453
LweI GCATC 3 cut(s) 51, 976, 1206
MaeI CTAG 3 cut(s) 585, 999, 1010
MalI GATC 3 cut(s) 757, 924, 1500
MboI GATC 3 cut(s) 755, 922, 1498
MboII GAAGA 6 cut(s) 117, 157, 440, 1161, 1188, 1376
MfeI CAATTG 1 cut(s) 627
MflI RGATCY 1 cut(s) 1498
MhlI GDGCHC 2 cut(s) 37, 609
MlsI TGGCCA 1 cut(s) 1448
MluCI AATT 8 cut(s) 91, 130, 267, 385, 627, 711, 875, 1085
MluNI TGGCCA 1 cut(s) 1448
MlyI GAGTC 1 cut(s) 1228
MmeI TCCRAC 1 cut(s) 1292
Mox20I TGGCCA 1 cut(s) 1448
MroI TCCGGA 1 cut(s) 497
MscI TGGCCA 1 cut(s) 1448
MseI TTAA 4 cut(s) 89, 150, 651, 1564
Msp20I TGGCCA 1 cut(s) 1448
MspCI CTTAAG 1 cut(s) 650
MspI CCGG 5 cut(s) 239, 290, 498, 1397, 1503
MspR9I CCNGG 7 cut(s) 178, 291, 732, 963, 1126, 1503, 1544
MunI CAATTG 1 cut(s) 627
Mva1269I GAATGC 1 cut(s) 1454
MvaI CCWGG 5 cut(s) 178, 732, 963, 1126, 1544
MwoI GCNNNNNNNGC 7 cut(s) 536, 566, 803, 995, 1019, 1247, 1256
NciI CCSGG 2 cut(s) 291, 1503
NcoI CCATGG 1 cut(s) 1449
NdeI CATATG 1 cut(s) 856
NdeII GATC 3 cut(s) 755, 922, 1498
NlaIII CATG 3 cut(s) 1210, 1234, 1453
NlaIV GGNNCC 5 cut(s) 237, 277, 606, 798, 1500
NmuCI GTSAC 5 cut(s) 108, 640, 895, 1141, 1147
NspV TTCGAA 2 cut(s) 128, 1536
PceI AGGCCT 2 cut(s) 547, 726
PctI GAATGC 1 cut(s) 1454
PfeI GAWTC 1 cut(s) 316
PflMI CCANNNNNTGG 1 cut(s) 1444
PfoI TCCNGGA 3 cut(s) 730, 961, 1542
PkrI GCNGC 7 cut(s) 331, 559, 571, 657, 796, 1021, 1468
PleI GAGTC 1 cut(s) 1228
PpsI GAGTC 1 cut(s) 1228
PpuMI RGGWCCY 1 cut(s) 1154
Psp124BI GAGCTC 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 1154
Psp6I CCWGG 5 cut(s) 176, 730, 961, 1124, 1542
PspFI CCCAGC 1 cut(s) 918
PspGI CCWGG 5 cut(s) 176, 730, 961, 1124, 1542
PspN4I GGNNCC 5 cut(s) 237, 277, 606, 798, 1500
PspPI GGNCC 5 cut(s) 4, 934, 1154, 1265, 1370
PspPPI RGGWCCY 1 cut(s) 1154
PstI CTGCAG 1 cut(s) 1024
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 1498
RsaI GTAC 2 cut(s) 277, 594
RsaNI GTAC 2 cut(s) 276, 593
SacI GAGCTC 1 cut(s) 37
SalI GTCGAC 1 cut(s) 229
SaqAI TTAA 4 cut(s) 89, 150, 651, 1564
SatI GCNGC 7 cut(s) 330, 558, 570, 656, 795, 1020, 1467
Sau3AI GATC 3 cut(s) 755, 922, 1498
Sau96I GGNCC 5 cut(s) 4, 934, 1154, 1265, 1370
SchI GAGTC 1 cut(s) 1228
ScrFI CCNGG 7 cut(s) 178, 291, 732, 963, 1126, 1503, 1544
SduI GDGCHC 2 cut(s) 37, 609
SfaNI GCATC 3 cut(s) 51, 976, 1206
SfcI CTRYAG 2 cut(s) 1020, 1242
SfuI TTCGAA 2 cut(s) 128, 1536
SgrDI CGTCGACG 1 cut(s) 229
SinI GGWCC 3 cut(s) 934, 1154, 1265
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
Sse9I AATT 8 cut(s) 91, 130, 267, 385, 627, 711, 875, 1085
SseBI AGGCCT 2 cut(s) 547, 726
SsiI CCGC 2 cut(s) 557, 794
SspI AATATT 1 cut(s) 773
SspMI CTAG 3 cut(s) 585, 999, 1010
SstI GAGCTC 1 cut(s) 37
StuI AGGCCT 2 cut(s) 547, 726
StyD4I CCNGG 7 cut(s) 176, 289, 730, 961, 1124, 1501, 1542
StyI CCWWGG 2 cut(s) 520, 1449
TaqI TCGA 5 cut(s) 128, 230, 925, 978, 1536
TasI AATT 8 cut(s) 91, 130, 267, 385, 627, 711, 875, 1085
TatI WGTACW 1 cut(s) 592
TauI GCSGC 2 cut(s) 560, 797
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 4 cut(s) 89, 150, 651, 1564
Tru9I TTAA 4 cut(s) 89, 150, 651, 1564
TscAI CASTG 3 cut(s) 649, 906, 1144
TseFI GTSAC 5 cut(s) 108, 640, 895, 1141, 1147
TseI GCWGC 5 cut(s) 329, 569, 655, 1019, 1466
Tsp45I GTSAC 5 cut(s) 108, 640, 895, 1141, 1147
TspDTI ATGAA 1 cut(s) 1223
TspRI CASTG 3 cut(s) 649, 906, 1144
Van91I CCANNNNNTGG 1 cut(s) 1444
Vha464I CTTAAG 1 cut(s) 650
VpaK11BI GGWCC 3 cut(s) 934, 1154, 1265
XapI RAATTY 4 cut(s) 91, 130, 267, 1085
XcmI CCANNNNNNNNNTGG 1 cut(s) 1282
XmiI GTMKAC 2 cut(s) 230, 357
XspI CTAG 3 cut(s) 585, 999, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.