RchiOBHm_Chr1g0371041

Autophagy-related protein 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0010981
Physical Location & Seq
Forward (+)
60404431 .. 60408872
4442 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 858 bp
ATGGTGTTCCGAGGCGATTCTCTAAAATGGGTTGTAGTCGCCTTGACGATTCTGCACCCGGTTGACCCGCTCTCTGCCAACTGCCATTTCAGTTTTGTTGGCGATTACAAGCTCTACGACTTCAGTTTGGATACTCCCATACCCTTGTATCCTCACGGAGTTCAAAGCGAAGATGGGTTCTACAAGGTCGTAGGAAATAAGACTGTGATCTGGTTTCAGCTTTGCGATGGAATGATTTTCAATCACGACCCACCTAGATGTGTTGATTGCTCGGATTGTGGTGGCCCATCACACTGTGGCATGGGTTGTAGTGCTCTTGTAGCAAAAAACACAGGAGGTTACGATGTTTGCACTAACATTGGGCGTGCCTCGAGCATGGACATTAACATAATTGATAAGAACAATCCTCACATTGGTGTCATTGTTAAGATGCAAGACTTTGTACCCAATAATATCTGTAGGTTGAAAAAATTTCTTACATGCCTGGTTCATGGTCTAGTTTCTAATCTTTTTATTTTGCAGGGACCATATTCGTTGGAGAAAACAGGGACATGTAACTATGGTACAGTGCTGAAGCATCCTTCTGGCTGTGCCAAGATTGTACATGTTAGTGGCAAAGGATGGGGCTGGTTTGCCACCTTTCTAATAATAGTCTTGTGCATTTTTGGAGTGTATCTGTTGGCTGGTGCAGTTTATCGATATTGTAGTCTTGGAGTTAGTGGAATAGATATTATACCAAATCTGGACTTTTGGACTGGCTTACCTCAGCAAGCACAAAGTGTGTCTGCTTCTTTATTGCACAAATTTAGGGGATCTTCTCAAGGTTATCGAAGCTCCTATTCTCCTGTCAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
ATG27

Protein Analysis

285

Amino Acids

31.21

Weight (kDa)

8.16

Isoelectric Point (pI)

30.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG27 PF09451 16 - 274 3.3e-16 Autophagy-related protein 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40316 AT2G40316 AT2G40316
fragaria_vesca FvH4_7g25140 FvH4_7g25140
malus_domestica MD01G1158200.v1.1 MD07G1226900.v1.1
prunus_persica Prupe.2G256500_v2.0.a1
pyrus_communis pycom01g17490 pycom07g20550
rosa_chinensis RchiOBHm_Chr1g0371041
rosa_laevigata RLG00000026990
rosa_multiflora Rmu_sc0001018.1_g000028
rosa_roxburghii Rroxscaffold_4G00286040
rosa_rugosa Rorug01G0361000 Rorug01G0361100
rosa_samantha Rh1AG371300 Rh1CG348100 Rh1DG366000
rosa_wichuraiana Rw1G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 70
AciI CCGC 1 cut(s) 68
AclWI GGATC 1 cut(s) 820
AcsI RAATTY 2 cut(s) 470, 803
AcuI CTGAAG 2 cut(s) 106, 593
AdeI CACNNNGTG 2 cut(s) 296, 779
AfaI GTAC 3 cut(s) 444, 565, 603
AfiI CCNNNNNNNGG 1 cut(s) 413
AflIII ACRYGT 2 cut(s) 551, 604
AgsI TTSAA 3 cut(s) 164, 241, 466
AjnI CCWGG 1 cut(s) 483
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AleI CACNNNNGTG 1 cut(s) 414
AluBI AGCT 3 cut(s) 112, 220, 834
AluI AGCT 3 cut(s) 112, 220, 834
Alw21I GWGCWC 1 cut(s) 316
AlwI GGATC 1 cut(s) 820
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 1 cut(s) 283
ApoI RAATTY 2 cut(s) 470, 803
AspS9I GGNCC 2 cut(s) 284, 524
AsuC2I CCSGG 1 cut(s) 59
AvaI CYCGRG 1 cut(s) 370
AvaII GGWCC 1 cut(s) 524
BaeI ACNNNNGTAYC 2 cut(s) 131, 164
Bbv12I GWGCWC 1 cut(s) 316
BbvCI CCTCAGC 1 cut(s) 765
BccI CCATC 4 cut(s) 167, 221, 295, 615
BciT130I CCWGG 1 cut(s) 485
BciVI GTATCC 2 cut(s) 124, 159
BcnI CCSGG 1 cut(s) 59
BfaI CTAG 2 cut(s) 255, 497
BfmI CTRYAG 1 cut(s) 457
BfuI GTATCC 2 cut(s) 124, 159
Bme1390I CCNGG 2 cut(s) 59, 485
Bme18I GGWCC 1 cut(s) 524
BmeT110I CYCGRG 1 cut(s) 370
BmgT120I GGNCC 2 cut(s) 284, 524
BmiI GGNNCC 1 cut(s) 525
BmrFI CCNGG 2 cut(s) 59, 485
BmsI GCATC 2 cut(s) 420, 586
Bpu10I CCTNAGC 1 cut(s) 765
BpuEI CTTGAG 1 cut(s) 804
BpuMI CCSGG 1 cut(s) 59
Bsa29I ATCGAT 1 cut(s) 697
BsaJI CCNNGG 1 cut(s) 10
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse1I ACTGG 1 cut(s) 760
BseBI CCWGG 1 cut(s) 485
BseCI ATCGAT 1 cut(s) 697
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 2 cut(s) 577, 626
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMII CTCAG 1 cut(s) 779
BseNI ACTGG 1 cut(s) 760
BsgI GTGCAG 2 cut(s) 38, 708
BshFI GGCC 1 cut(s) 285
BshVI ATCGAT 1 cut(s) 697
BsiHKAI GWGCWC 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 370
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 2 cut(s) 537, 562
BslI CCNNNNNNNGG 1 cut(s) 413
BsmFI GGGAC 2 cut(s) 537, 562
BsnI GGCC 1 cut(s) 285
BsoBI CYCGRG 1 cut(s) 370
Bsp1286I GDGCHC 1 cut(s) 316
Bsp1407I TGTACA 1 cut(s) 601
Bsp143I GATC 2 cut(s) 207, 812
BspACI CCGC 1 cut(s) 68
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 1 cut(s) 778
BspDI ATCGAT 1 cut(s) 697
BspLI GGNNCC 1 cut(s) 525
BspPI GGATC 1 cut(s) 820
BsrBI CCGCTC 1 cut(s) 70
BsrGI TGTACA 1 cut(s) 601
BsrI ACTGG 1 cut(s) 760
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 2 cut(s) 207, 812
Bst2UI CCWGG 1 cut(s) 485
Bst4CI ACNGT 3 cut(s) 205, 296, 568
BstAUI TGTACA 1 cut(s) 601
BstC8I GCNNGC 2 cut(s) 366, 771
BstDEI CTNAG 1 cut(s) 765
BstF5I GGATG 2 cut(s) 577, 626
BstKTI GATC 2 cut(s) 210, 815
BstMBI GATC 2 cut(s) 207, 812
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstNI CCWGG 1 cut(s) 485
BstNSI RCATGY 3 cut(s) 483, 555, 608
BstSCI CCNGG 2 cut(s) 57, 483
BstSFI CTRYAG 1 cut(s) 457
BstX2I RGATCY 1 cut(s) 812
BstYI RGATCY 1 cut(s) 812
Bsu15I ATCGAT 1 cut(s) 697
BsuI GTATCC 2 cut(s) 124, 159
BsuRI GGCC 1 cut(s) 285
BsuTUI ATCGAT 1 cut(s) 697
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 2 cut(s) 577, 626
BtsIMutI CAGTG 2 cut(s) 292, 573
Cac8I GCNNGC 2 cut(s) 366, 771
Cfr13I GGNCC 2 cut(s) 284, 524
ClaI ATCGAT 1 cut(s) 697
Csp6I GTAC 3 cut(s) 443, 564, 602
CviAII CATG 6 cut(s) 301, 376, 480, 491, 552, 605
CviJI RGCY 8 cut(s) 112, 220, 285, 588, 627, 683, 759, 834
CviKI_1 RGCY 8 cut(s) 112, 220, 285, 588, 627, 683, 759, 834
CviQI GTAC 3 cut(s) 443, 564, 602
DdeI CTNAG 1 cut(s) 765
DpnI GATC 2 cut(s) 209, 814
DpnII GATC 2 cut(s) 207, 812
DraIII CACNNNGTG 2 cut(s) 296, 779
Eco47I GGWCC 1 cut(s) 524
Eco57I CTGAAG 2 cut(s) 106, 593
Eco88I CYCGRG 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 483
FaeI CATG 6 cut(s) 304, 379, 483, 494, 555, 608
FaqI GGGAC 2 cut(s) 537, 562
FatI CATG 6 cut(s) 300, 375, 479, 490, 551, 604
FauI CCCGC 1 cut(s) 75
FokI GGATG 2 cut(s) 564, 633
FspBI CTAG 2 cut(s) 255, 497
HaeIII GGCC 1 cut(s) 285
HapII CCGG 1 cut(s) 59
Hin1II CATG 6 cut(s) 304, 379, 483, 494, 555, 608
HincII GTYRAC 2 cut(s) 64, 850
HindII GTYRAC 2 cut(s) 64, 850
HinfI GANTC 2 cut(s) 17, 49
HpaII CCGG 1 cut(s) 59
Hpy166II GTNNAC 2 cut(s) 64, 850
Hpy188I TCNGA 3 cut(s) 11, 274, 857
Hpy188III TCNNGA 2 cut(s) 245, 743
Hpy8I GTNNAC 2 cut(s) 64, 850
HpyAV CCTTC 1 cut(s) 591
HpyCH4III ACNGT 3 cut(s) 205, 296, 568
HpyCH4V TGCA 7 cut(s) 55, 351, 433, 520, 660, 689, 799
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpyF3I CTNAG 1 cut(s) 765
Hsp92II CATG 6 cut(s) 304, 379, 483, 494, 555, 608
Kzo9I GATC 2 cut(s) 207, 812
LmnI GCTCC 1 cut(s) 839
LweI GCATC 2 cut(s) 420, 586
MaeI CTAG 2 cut(s) 255, 497
MaeIII GTNAC 2 cut(s) 338, 554
MalI GATC 2 cut(s) 209, 814
MbiI CCGCTC 1 cut(s) 70
MboI GATC 2 cut(s) 207, 812
MboII GAAGA 2 cut(s) 182, 807
MflI RGATCY 1 cut(s) 812
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 3 cut(s) 390, 470, 803
MmeI TCCRAC 1 cut(s) 516
MnlI CCTC 6 cut(s) 5, 162, 329, 379, 417, 774
MseI TTAA 2 cut(s) 384, 426
MslI CAYNNNNRTG 3 cut(s) 256, 414, 609
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 2 cut(s) 59, 485
MvaI CCWGG 1 cut(s) 485
MwoI GCNNNNNNNGC 1 cut(s) 320
NciI CCSGG 1 cut(s) 59
NdeII GATC 2 cut(s) 207, 812
NlaIII CATG 6 cut(s) 304, 379, 483, 494, 555, 608
NlaIV GGNNCC 1 cut(s) 525
NspI RCATGY 3 cut(s) 483, 555, 608
OliI CACNNNNGTG 1 cut(s) 414
PaeR7I CTCGAG 1 cut(s) 370
PciI ACATGT 2 cut(s) 551, 604
PfeI GAWTC 2 cut(s) 17, 49
PscI ACATGT 2 cut(s) 551, 604
Psp6I CCWGG 1 cut(s) 483
PspGI CCWGG 1 cut(s) 483
PspN4I GGNNCC 1 cut(s) 525
PspPI GGNCC 2 cut(s) 284, 524
PspXI VCTCGAGB 1 cut(s) 370
PsuI RGATCY 1 cut(s) 812
RsaI GTAC 3 cut(s) 444, 565, 603
RsaNI GTAC 3 cut(s) 443, 564, 602
RseI CAYNNNNRTG 3 cut(s) 256, 414, 609
SaqAI TTAA 2 cut(s) 384, 426
Sau3AI GATC 2 cut(s) 207, 812
Sau96I GGNCC 2 cut(s) 284, 524
ScrFI CCNGG 2 cut(s) 59, 485
SduI GDGCHC 1 cut(s) 316
SfaNI GCATC 2 cut(s) 420, 586
SfcI CTRYAG 1 cut(s) 457
Sfr274I CTCGAG 1 cut(s) 370
SinI GGWCC 1 cut(s) 524
SlaI CTCGAG 1 cut(s) 370
SmiMI CAYNNNNRTG 3 cut(s) 256, 414, 609
SmlI CTYRAG 2 cut(s) 370, 819
SmoI CTYRAG 2 cut(s) 370, 819
Sse9I AATT 3 cut(s) 390, 470, 803
SsiI CCGC 1 cut(s) 68
SspMI CTAG 2 cut(s) 255, 497
StyD4I CCNGG 2 cut(s) 57, 483
TaaI ACNGT 3 cut(s) 205, 296, 568
TaqI TCGA 3 cut(s) 371, 697, 829
TasI AATT 3 cut(s) 390, 470, 803
TatI WGTACW 1 cut(s) 601
TfiI GAWTC 2 cut(s) 17, 49
Tru1I TTAA 2 cut(s) 384, 426
Tru9I TTAA 2 cut(s) 384, 426
TscAI CASTG 2 cut(s) 299, 573
TspDTI ATGAA 1 cut(s) 479
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 2 cut(s) 299, 573
VpaK11BI GGWCC 1 cut(s) 524
XapI RAATTY 2 cut(s) 470, 803
XceI RCATGY 3 cut(s) 483, 555, 608
XhoI CTCGAG 1 cut(s) 370
XspI CTAG 2 cut(s) 255, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.